不同水平&alpha;-半乳糖苷酶对1~21日龄爱拔益加肉鸡生长性能的影响及相关机理研究

林谦; 蒋桂韬; 张旭; 王向荣; 戴求仲

doi:10.3969/j.issn.1006-267x.2015.08.019

动物营养学报 >

2015 , Vol. 27 >Issue 8: 2466 - 2475

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.08.019

饲料营养 Feed Science and Technology

不同水平α-半乳糖苷酶对1~21日龄爱拔益加肉鸡生长性能的影响及相关机理研究

林谦 ,
蒋桂韬 ,
张旭 ,
王向荣 ,
戴求仲

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1. 中国农业科学院麻类研究所, 长沙 410205;
2. 湖南农业大学动物科学技术学院, 长沙 410128;
3. 湖南省畜牧兽医研究所, 长沙 410131

收稿日期: 2015-03-24

网络出版日期: 2015-08-13

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Effects of Different α-Galactosidase Levels on Growth Performance of AA Broilers Aged from 1 to 21 Days and Its Mechanism Research

LIN Qian ,
JIANG Guitao ,
ZHANG Xu ,
WANG Xiangrong ,
DAI Qiuzhong

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1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China;
2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
3. Department of Animal Nutrition and Feeding Technology, Hunan Institute of Animal Science and Veterinary Medicine, Changsha 410131, China

Received date: 2015-03-24

Online published: 2015-08-13

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摘要

本试验旨在研究饲粮添加不同水平α-半乳糖苷酶对1~21日龄爱拔益加(AA)肉鸡生长性能、肠道黏膜形态结构和血清生化、激素指标的影响。试验选择1日龄体重相近的健康AA肉鸡360羽,随机分成6个处理,每处理5个重复,每重复12羽,处理A饲喂玉米-豆粕型基础饲粮作为正对照,处理B饲喂降低基础饲粮代谢能0.21 MJ/kg的饲粮作为负对照,处理C、D、E、F分别饲喂在负对照处理饲粮中添加75、150、300、600 g/t的α-半乳糖苷酶制剂(酶活为400 U/g)的饲粮。试验期为21 d。结果表明:1)饲粮中添加不同水平α-半乳糖苷酶对1~21日龄各处理各阶段AA肉鸡平均日增重(ADG)、平均日采食量(ADFI)、料重比(F/G)以及1~21日龄试鸡存活率均无显著影响(P>0.05),但饲粮添加75 g/t的α-半乳糖苷酶(处理C)有改善1~21日龄AA肉鸡F/G的趋势(0.10 >P >0.05);2)酶制剂的添加不同程度地促进了1~21日龄AA肉鸡十二指肠、空肠和回肠黏膜形态结构的发育;3)饲粮中添加不同水平α-半乳糖苷酶较正、负对照处理对21日龄AA肉鸡血清总蛋白、球蛋白含量和白蛋白/球蛋白影响显著(P<0.05),同时处理C、E、F较负对照处理对21日龄AA肉鸡血清胰岛素含量有极显著提高(P<0.01),处理C和E试鸡21日龄血清胰高血糖素含量较正对照处理亦有极显著提高(P<0.01)。综合分析生长性能、肠道黏膜形态结构和血清生化、激素指标结果,在本试验条件下,在降低基础饲粮代谢能0.21 MJ/kg的基础上,添加75 g/t的α-半乳糖苷酶制剂即可使1~21日龄AA肉鸡生长性能达到与饲喂基础饲粮相当的水平。

关键词： α-半乳糖苷酶; 生长性能; 肠道黏膜形态结构; 血清生化指标; 血清激素指标; 爱拔益加肉鸡

本文引用格式

林谦 , 蒋桂韬 , 张旭 , 王向荣 , 戴求仲 . 不同水平α-半乳糖苷酶对1~21日龄爱拔益加肉鸡生长性能的影响及相关机理研究[J]. 动物营养学报, 2015 , 27(8) : 2466 -2475 . DOI: 10.3969/j.issn.1006-267x.2015.08.019

Abstract

This study was conducted to evaluate the effects of different levels of α-galactosidase on growth performance, intestinal mucosa structure, and serum biochemical and hormonal indices of Arbor Acre (AA) broilers aged from 1 to 21 days. A total of 360 healthy 1-day-old AA broilers with the nearly same weight were randomly selected and allocated to 6 treatments, 5 replicates per treatment and 12 broilers per replication. The positive control treatment was provided with the corn-soybean basal diet, the negative control treatment was fed the basal diet which was reduced 0.21 MJ/kg ME, and the test treatments C, D, E and F were fed the negative control diet which were added 75, 150, 300, 600 g/t α-galactosidase (enzyme activities was 400 U/g), respectively. The experiment period was 21 d. The results showed as follows: 1) there was no significant difference on average daily gain (ADG), average daily feed intake (ADFI), the ratio of feed to gain (F/G) and survival rate during the whole experiment period (P>0.05), but there was a trend that the addition of 75 g/t α-galactosidase (treatment C) decreased F/G of AA broilers aged from 1 to 21 days (0.10> P> 0.05). 2) The addition of α-galactosidase could improve the mucosa structure development of duodenum, jejunum and ileum in some extent. 3) Compared with treatments A and B, Added different levels of α-galactosidase in diet significantly affected the contents of serum total protein (TP), globulin (GLO) and the ratio of albumin to globulin (ALB/GLO) on 21-day-old broilers (P<0.05). And at the same time, compared with treatments B, treatments C, E and F significantly improved the content of serum insulin of 21-day-old broilers (P<0.01), and compared with treatment A, treatments C and E also significantly improved the content of glucagon (GLU) of 21-day-old broilers (P<0.01). In general, added 75 g/t α-galactosidase had a good effect when AA broilers aged from 1 to 21 d fed the diet which was reduced 0.21 MJ/kg ME.

Key words： α-galactosidase; growth performance; intestinal mucosa structure; serum biochemical indices; serum hormonal indices; AA broiler

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