动物营养学报 >

2015 , Vol. 27 >Issue 9: 2742 - 2748

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.09.012

反刍动物营养 Ruminant Nutrition

低饲粮阴阳离子差协同维生素D对黔北麻羊妊娠母羊血浆钙及其调控因子浓度的影响

  • 朱伦琴 ,
  • 田兴舟 ,
  • 袁鑫 ,
  • 杨春红 ,
  • 莘海亮 ,
  • 赵文金 ,
  • 吴文旋
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  • 1. 贵州大学动物科学学院, 贵阳 550025;
    2. 黔东南民族职业技术学院, 凯里 556000;
    3. 习水富兴牧业有限公司, 习水 564600;
    4. 贵州大学新农村发展研究院·中国西部发展能力 研究中心, 贵阳 550025

收稿日期: 2015-03-26

  网络出版日期: 2015-09-12

基金资助

国家自然科学基金(31360563,31160468)

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Synergy Effects of Low Dietary Cation-Anion Difference with Vitamin D on Plasma Concentrations of Calcium and Its Modulators of Pregnant Goats

  • ZHU Lunqin ,
  • TIAN Xingzhou ,
  • YUAN Xin ,
  • YANG Chunhong ,
  • XIN Hailiang ,
  • ZHAO Wenjin ,
  • WU Wenxuan
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  • 1. College of Animal Science, Guizhou University, Guiyang 550025, China;
    2. Qiandongnan National Polytechnic, Kaili 556000, China;
    3. Fuxing Husbandry Co., Ltd., Xishui County, Xishui 564600, China;
    4. Institute of New Rural Development·China Center for Western Development Capacity Research, Guizhou University, Guiyang 550025, China

Received date: 2015-03-26

  Online published: 2015-09-12

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摘要

本文旨在研究低饲粮阴阳离子差(DCAD)条件下添加维生素D对黔北麻羊妊娠母羊血浆钙及其调控因子浓度的影响。将18头健康妊娠母羊随机分为3组,每组6个重复,每个重复1只羊,各组分别饲喂DCAD为212.4(对照)、-145.5(Ⅰ)、-145.5 mmol/kg(Ⅱ)(干物质基础,实测值)的饲粮。其中,对照组饲喂基础饲粮,Ⅰ组在对照组基础上添加45 g/d六水合氯化镁,Ⅱ组在Ⅰ组基础上添加2 000 IU/d维生素D。预试期10 d,正试期30 d。结果表明:1)Ⅰ、Ⅱ组尿液pH显著低于对照组(P<0.05)。3组山羊生长性能不受饲粮处理的影响(P>0.05)。2)与对照组相比,Ⅰ(P>0.05)、Ⅱ组(P<0.05)血浆钙浓度均升高;对照组、Ⅰ、Ⅱ组血浆磷、甲状旁腺激素、降钙素浓度没有显著差异(P>0.05),Ⅱ组较Ⅰ组和对照组显著提高了血浆1,25二羟基维生素D3浓度(P<0.05)。3)Ⅱ组血浆维生素D受体、钙结合蛋白D9k浓度显著高于对照组(P<0.05)。在本试验条件下,在低DCAD条件下添加维生素D能更有助于维持动物血液钙恒稳状态。

关键词： 饲粮阴阳离子差; 维生素D受体; 钙结合蛋白D9k; 血浆钙浓度

本文引用格式

朱伦琴 , 田兴舟 , 袁鑫 , 杨春红 , 莘海亮 , 赵文金 , 吴文旋 . 低饲粮阴阳离子差协同维生素D对黔北麻羊妊娠母羊血浆钙及其调控因子浓度的影响[J]. 动物营养学报, 2015 , 27(9) : 2742 -2748 . DOI: 10.3969/j.issn.1006-267x.2015.09.012

Abstract

This study was conducted to evaluate the synergy effects of low dietary cation-anion difference (DCAD) with vitamin D on plasma concentrations of calcium (Ca) and its modulators of pregnant goats. Eighteen healthy pregnant goats were randomly allocated to 3 blocks with 6 replicates per group and 1 goat per replicate. Goats were fed 3 DCAD at 212.4 (control), -145.5 (Ⅰ), -145.5 mmol/kg (Ⅱ) [dry matter (DM) basis, measured values], respectively. Control group was fed a basal diet, 45 g/d MgCl2·6H2O was added in group Ⅰ on the basis of the basal diet, and 2 000 IU/d vitamin D was added in group Ⅱ on the basis of group Ⅰ. The pre-test lasted for 10 d, and the test lasted for 30 d. The results showed as follow: 1) urine pH was significantly reduced in groups Ⅰ and Ⅱ over control (P<0.05). For the growth performance measurements DM intake, average daily gain, and feed to gain ratio were not affected by dietary treatments (P>0.05). 2) Compared with control group, plasma Ca concentration was increased in groups Ⅰ (P>0.05) and Ⅱ (P<0.05); there were no significant differences in plasma phosphorus, parathormone and calcitonin concentrations for control group and groups Ⅰ and Ⅱ (P>0.05), but group Ⅱ significantly increased plasma 1,25-(OH)2VD3 concentration compared with control group and group Ⅰ (P<0.05). 3) Plasma vitamin D receptor and Ca-binding protein-D9k concentrations in group Ⅱ were significantly increased compared with control group (P<0.05). These results suggest that blood Ca homeostasis can be more available to maintain by low DCAD synergized with vitamin D under the conditions in the present study.

Key words： dietary cation-anion difference; vitamin D receptor; Ca-binding protein-D9k; plasma Ca concentration

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