动物营养学报 >

2015 , Vol. 27 >Issue 9: 2811 - 2819

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.09.019

饲料营养 Feed Science and Technology

饲粮添加复合抗菌肽与包被氧化锌对断奶仔猪生长性能及血清生化指标的影响

  • 李方方 ,
  • 苏航 ,
  • 张勇 ,
  • 况应谷 ,
  • 高原 ,
  • 孟玲 ,
  • 郭福来 ,
  • 朱宇旌
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  • 1. 沈阳农业大学畜牧兽医学院, 沈阳 110866;
    2. 福建深纳生物工程有限公司, 福建 350700;
    3. 辽宁德宝农牧集团有限公司, 沈阳 110171

收稿日期: 2015-03-23

  网络出版日期: 2015-09-12

基金资助

国家自然科学基金(31440082,31101253)

收起

Effects of Dietary Anti-Microbial Peptides and Coated Zinc Oxide on Growth Performance and Serum Biochemical Indices of Weaning Piglets

  • LI Fangfang ,
  • SU Hang ,
  • ZHANG Yong ,
  • KUANG Yinggu ,
  • GAO Yuan ,
  • MENG Ling ,
  • GUO Fulai ,
  • ZHU Yujing
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  • 1. College of Animal Sciences and Veterinary Sciences, Shenyang Agricultural University, Shenyang 110866, China;
    2. Fujian Shenna Bio Engineering Co., Ltd., Fujian 350700, China;
    3. Liaoning Debao Argi-animal Husbandry Co., Ltd., Shenyang 110171, China

Received date: 2015-03-23

  Online published: 2015-09-12

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摘要

本试验旨在研究饲粮添加复合抗菌肽(C-AMPs)与包被氧化锌(C-ZnO)对断奶仔猪生长性能、血清生化指标、养分表观消化率及粪中微生物菌群的影响。试验选用平均体重为(6.98±0.05) kg的大白仔猪160头,随机分为4组,每组4个重复,每个重复10头猪。各组分别饲喂基础饲粮(对照组)、基础饲粮+0.5 mg/kg C-AMPs(C-AMPs组)、基础饲粮+1 800 mg/kg C-ZnO(C-ZnO组)、基础饲粮+0.5 mg/kg C-AMPs+1 800 mg/kg C-ZnO(C-AMPs+C-ZnO组)。试验期28 d。结果表明:1)与对照组相比,饲粮添加C-AMPs、C-ZnO以及二者共同添加可显著或极显著提高断奶仔猪的平均日增重及平均日采食量(P<0.05或P<0.01);极显著降低断奶仔猪腹泻率(P<0.01)。2)与对照组相比,饲粮添加C-AMPs、C-ZnO血清生长激素含量显著升高(P<0.05),血清碱性磷酸酶活性显著降低(P<0.05);饲粮C-AMPs和C-ZnO共同添加血清免疫球蛋白G、生长激素、白细胞介素2和白细胞介素6含量极显著提高(P<0.01);饲粮添加C-AMPs血清猪瘟抗体含量极显著提高(P<0.01)。3)与对照组相比,饲粮添加C-ZnO显著提高粗蛋白质、粗脂肪和有机物表观消化率(P<0.05)。4)与对照组相比,饲粮添加C-AMPs、C-ZnO以及二者共同添加粪中大肠杆菌数量极显著减少(P<0.01),双歧杆菌数量极显著增加(P<0.01)。综合分析,饲粮中添加C-AMPs与C-ZnO可提高断奶仔猪的生长性能和养分表观消化率,调节相关免疫因子,调节肠道菌群平衡。

关键词： 复合抗菌肽; 包被氧化锌; 断奶仔猪; 生长性能; 血清生化指标; 消化率; 微生物菌群

本文引用格式

李方方 , 苏航 , 张勇 , 况应谷 , 高原 , 孟玲 , 郭福来 , 朱宇旌 . 饲粮添加复合抗菌肽与包被氧化锌对断奶仔猪生长性能及血清生化指标的影响[J]. 动物营养学报, 2015 , 27(9) : 2811 -2819 . DOI: 10.3969/j.issn.1006-267x.2015.09.019

Abstract

This experiment was conducted to investigate the effects of dietary anti-microbial peptides (C-AMPs) and coated zinc oxide (C-ZnO) on growth performances, serum biochemical indices, nutrient apparent digestibility and feacal microflora of weaning piglets. A total of 160 large white piglets with body weight of (6.98±0.05) kg were randomly allotted to 4 groups with 4 replicates per group and 10 piglets per replicate. The four groups were fed the basal diet, the basal diet+0.5 mg/kg C-AMPs (C-AMPs group), the basal diet+1 800 mg/kg C-ZnO (C-ZnO group) and the basal diet+0.5 mg/kg C-AMPs+1 800 mg/kg C-ZnO (C-AMPs+C-ZnO group). The trial lasted for 28 days. The results showed as follows:1) compared with the control group, dietary supplemented with C-AMPs and C-ZnO or two together, the average daily gain and average daily feed intake significantly increased (P<0.05 or P<0.01), the diarrhea rate of weaning piglets significantly reduced (P<0.01). 2) Compared with the control group, dietary supplemented with C-AMPs and C-ZnO significantly increased serum growth hormone content (P<0.05), significantly decreased serum alkaline phosphatase activity (P<0.05); dietary supplemented with both C-AMPs and C-ZnO significantly increased the content of immunoglobulin G, growth hormone, interleukin 2 and interleukin 6 in serum (P<0.01), dietary supplemented with C-AMPs significantly increased serum CSF-Ab content (P<0.01). 3) Compared with the control group, dietary supplemented with C-ZnO significantly increased the apparent digestibility of crude protein, ether extract and organism matter (P<0.05). 4) Compared with the control group, dietary supplemented with C-AMPs and C-ZnO or two together significantly reduced the number of E.coli in excrement (P<0.01), but the number of Bifidobacterium significant increased (P<0.01). The results indicate that supplementation of C-AMPs and C-ZnO in the diet can improve the growth performance and nutrient apparent digestibility, also can regulate the immune related and the balance of intestinal flora.

Key words： compound antimicrobial peptides; coated ZnO; weaning piglets; growth performance; serum biochemical indices; digestbility; microflora

参考文献

[1] BROGDEN K A.Antimicrobial peptides:pore formers or metabolic inhibitors in bacteria?[J].Nature Reviews Microbiology,2005,3(3):238-250.  

[2] 袁威,任志华,邓又天,等.复合抗菌肽对断奶仔猪生长性能及血清细胞因子含量的影响[J].动物营养学报,2015,27(3):885-892.

[3] SINGH D P.Synthesis and growth of ZnO nanowires[J].Science of Advanced Materials,2010,2(3):245-272.  

[4] VALLEE B L,FALCHUK K H.The biochemical basis of zinc physiology[J].Physiological Reviews,1993,73(1):79-118.

[5] DAVIS M E,BROWN D C,MAXWELL C V,et al.Effect of phosphorylated mannans and pharmacological additions of zinc oxide growth and immunocompetence of weanling pigs[J].Journal of Animal Science,2004,82(2):581-587.

[6] SMITH J W,TOKACH M D,GOODBAND R D,et al.Effects of the interrelationship between zinc oxide and copper sulfate on growth performance of early-weaned pigs[J].Journal of Animal Science,1997,75(7):1861-1866.

[7] HILL G M,GROMWELL G L,CRENSHAW T D,et al.Growth promotion effects and plasma changes from feeding high dietary concentrations of zinc and copper to weanling pigs (regional study)[J].Journal of Animal Science,2000,78(4):1010-1016.

[8] XIONG X,YANG H S,LI L,et al.Effects of antimicrobial peptides in nursery diets on growth performance of pigs reared on five different farms[J].Livestock Science,2014,167(9):206-210.

[9] 王建华,伍淳操.抗菌肽和蛋氨酸锌对断奶仔猪部分血液常规指标的影响[J].贵州农业科学,2010,38(3):127-130.

[10] 赵燕飞,汪以真,林文学.锌对断奶后不同阶段仔猪抗菌肽PR-39 mRNA表达的影响[J].中国兽医杂志,2005,41(4):10-13.

[11] SHAY N F,COUSINS R J.Cloning of rat intestinal mRNAs affected by zinc deficiency[J].The Journal of Nutrition,1993,123(1):35-41.

[12] YOON J H,INGALE S L,KIM J S,et al.Effects of dietary supplementation with antimicrobial peptide-P5 on growth performance,apparent total tract digestibility,faecal and intestinal microflora and intestinal morphology of weanling pigs[J].Journal of the Science of Food and Agriculture,2013,93(3):587-592.  

[13] 张晓杰,李玉侠,卢志勇.日粮中添加不同种类抗菌肽对仔猪生产性能的影响[J].饲料博览,2013(2):25-27.

[14] YOUSEF J M,DANIAL E N.In vitro antibacterial activity and minimum inhibitory concentration of zinc oxide and nano-particle zinc oxide against pathogenic strains[J].Journal of Health Sciences,2012,2(4):38-42.

[15] AGERBERTH B,LEE J Y,BERGMAN T,et al.Amino acid sequence of PR-39[J].European Journal of Biochemistry,1991,202(3):849-854.  

[16] AGERBERTH B,BOMAN A,ANDERSSON M,et al.Isolation of three antibacterial peptides from pig intestine:gastric inhibitory polypeptide (7-42),diazepam-binding inhibitor (32-86) and a novel factor,peptide 3910[J].European Journal of Biochemistry,1993,216(2):623-629.  

[17] ANDERSSON M,GUNNE H,AGERBERTH B,et al.NK-lysin,a novel effector peptide of cytotoxic T and NK cells.Structure and cDNA cloning of the porcine form,induction by interleukin 2,antibacterial and antitumour activity[J].The EMBO Journal,1995,14(8):1615-1625.

[18] LEE J Y,BOMAN A,SUN C X,et al.Antibacterial peptides from pig intestine:isolation of a mammalian cecropin[J].Proceedings of the National Academy of Sciences of the United States of America,1989,86(23):9159-9162.  

[19] 邵斌,张彩霞,赵茹茜,等.运输对仔猪抗氧化功能和促炎细胞因子产生的影响[J].畜牧兽医学报,2009,40(10):1545-1549.

[20] COMA J,ZIMMERMAN D R,CARRION D.Relationship of rate of lean tissue growth and other factors to concentration of urea in plasma of pigs[J].Journal of Animal Science,1995,73(12):3649-3656.

[21] 马文锋,焦喜兰,李振田,等.抗菌肽替代氧化锌对仔猪生产性能及血清猪瘟抗体水平的影响[J].中国饲料,2011(19):22-24.
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