研究简报 Short Communications

饲粮蛋白质水平对家兔食欲肽基因表达的影响

  • 隋啸一 ,
  • 高琴 ,
  • 刘磊 ,
  • 李福昌
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  • 山东农业大学动物科技学院, 泰安 271018

收稿日期: 2015-03-26

  网络出版日期: 2015-09-12

基金资助

现代农业产业技术体系建设专项(CARS-44-B-1);山东省农业重大应用技术创新课题——肉、獭兔高效高产技术研究、集成与推广(2015—2016);山东农业大学青年科技创新基金(2015—2016)

Effects of Dietary Protein Level on Appetitive Peptide Gene Expression of Rabbits

  • SUI Xiaoyi ,
  • GAO Qin ,
  • LIU Lei ,
  • LI Fuchang
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  • College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2015-03-26

  Online published: 2015-09-12

摘要

本试验旨在探讨饲粮蛋白质水平对家兔食欲肽基因表达的影响。选择体重相近的30日龄断奶伊拉肉兔30只,随机分为3组,分别饲喂蛋白质水平为12.8%、16.0%和19.2%的试验饲粮,每组10个重复,每个重复1只兔。预试期7 d,正试期35 d。试验结束后,采集弓形核、十二指肠、空肠和回肠,测定弓形核中阿片促黑色素原(POMC)、可卡因-苯丙胺调节转录物(CART)、神经肽Y(NPY)、刺鼠基因相关蛋白(AgRP)、胆囊收缩素(CCK)和肠道中CCK基因的相对表达量。结果显示:十二指肠CCK基因的相对表达量显著高于空肠、回肠和弓形核(P0.05);与16.0%和12.8%蛋白质饲粮组相比,19.2%蛋白质饲粮组弓形核中POMCCART基因的相对表达量显著增加(P0.05)。由此得出,饲喂低蛋白质水平(12.8%~16.0%)饲粮家兔的食欲增加与肠道和中枢中脑肠肽有关。与低蛋白质水平饲粮相比,高蛋白质水平(19.2%)饲粮显著提高十二指肠中CCK和弓形核中CARTPOMC基因的相对表达量,表明这些基因形成食欲网络共同参与饲粮蛋白质调控家兔食欲的过程。此外,回肠和弓形核中CCK、弓形核中NPYAgRP基因对饲粮中蛋白质水平变化不敏感。

本文引用格式

隋啸一 , 高琴 , 刘磊 , 李福昌 . 饲粮蛋白质水平对家兔食欲肽基因表达的影响[J]. 动物营养学报, 2015 , 27(9) : 2947 -2954 . DOI: 10.3969/j.issn.1006-267x.2015.09.035

Abstract

This experiment was conducted to investigate the effects of dietary protein level on appetitive peptide gene expression of rabbits. Thirty 30-day-old weaning Ira rabbits with the similar weight were randomly divided into 3 groups (10 repelicates per group and 1 rabbit per repelicate), which were fed the experimental diets with protein levels were 12.8%, 16.0% and 19.2%, respectively. The trial lasted for 7 days for adaptation, and 35 days for trial. After trial, arcuate nucleus (ARC), duodenum, jejunum and ileum were sampled to measure the relative expression levels of proopiomelanocortin (POMC), cocaine-amphetamine (CART), neuropeptide Y (NPY), agouti gene-related protein (AgRP), cholecystokinin (CCK) genes in ARC and CKK gene in intestine. The results showed as follows: the relative expression level of CCK gene in duodenum was significantly higher than that in jejunum, ileum and ARC (P0.05). Compared with 12.8% and 16.0% protein diet groups, the relative expression levels of POMC and CART genes in ARC of 19.2% protein diet group were significantly increased (P0.05). The results suggest that the rabbits with low protein level (12.8% to 16.0%) diets have higher appetite that is relative to the brain-gut neuropeptides. Besides, relative expression levels of CCK gene in duodenum, CART and POMC genes in ARC of high protein level (19.2%) diet are significantly higher than those of low protein level diets, which indicates that the appetite network formed by CCK gens in duodenum, CART and POMC genes in ARC are involved in the regulation process of dietary protein on appetite of rabbits. But CCK gene in ileum and ARC, NPY and AgRP genes in ARC are not sensitive to the dietary protein level variation.

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