硫酸钠对蛋鸡生产性能、抗氧化能力、肠道形态及盲肠微生物菌群的影响

熊平文; 刘兵; 陈娜娜; 何俊娜; 余东游

doi:10.3969/j.issn.1006-267x.2015.11.007

动物营养学报 >

2015 , Vol. 27 >Issue 11: 3358 - 3365

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.11.007

禽营养 Poultry Nutrition

硫酸钠对蛋鸡生产性能、抗氧化能力、肠道形态及盲肠微生物菌群的影响

熊平文 ,
刘兵 ,
陈娜娜 ,
何俊娜 ,
余东游

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浙江大学动物科学学院, 农业部华东动物营养与饲料重点实验室, 杭州 310058

收稿日期: 2015-06-09

网络出版日期: 2015-11-21

基金资助

东南沿海地区大学农业科技服务技术集成与示范(2013BAD20B02)

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Effects of Sodium Sulfate on Performance, Antioxidant Capacity, Intestinal Morphology and Cecal Microflora Population of Laying Hens

XIONG Pingwen ,
LIU Bing ,
CHEN Na ,
HE Junna ,
YU Dongyou

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Key Laboratory of Animal Nutrition and Feed in East China of Ministry of Agriculture, College of Animal Science, Zhejiang University, Hangzhou 310058, China

Received date: 2015-06-09

Online published: 2015-11-21

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摘要

本试验旨在研究饲粮中添加硫酸钠对蛋鸡生产性能、抗氧化能力、肠道形态及盲肠微生物菌群的影响。选取健康且体重和产蛋率相近的20周龄海兰白蛋鸡450只,随机分为5 组,每组6个重复,每个重复15只鸡。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加0.3%、0.6%、1.5%、3.0%硫酸钠的试验饲粮。试验期8周。结果显示:1)饲粮中添加硫酸钠可促进蛋鸡产蛋,其中以低剂量(0.3%和0.6%)组效果较佳,但差异不显著(P>0.05)。2)与对照组相比,饲粮中添加硫酸钠对血浆和肝脏的抗氧化指标均无显著影响(P>0.05)。与0.6%组相比,高剂量(1.5%和3.0%)组血浆的谷胱甘肽过氧化物酶(GSH-Px)活性和肝脏的总超氧化物歧化酶(T-SOD)活性显著或极显著降低(P<0.05或P<0.01),3.0%组的血浆总抗氧化能力(T-AOC)和肝脏GSH-Px活性显著降低(P<0.05)。3)与对照组相比,0.6%组十二指肠和回肠的绒毛高度分别提高了30.51%和42.63%(P<0.01),0.6%组十二指肠的隐窝深度显著降低(P<0.05),绒隐比显著提高(P<0.05)。与0.6%组相比,高剂量(1.5%和3.0%)组十二指肠和回肠的绒毛高度及十二指肠的绒隐比极显著降低(P<0.01),3.0%组十二指肠和回肠的隐窝深度极显著增加(P<0.01)。4)与对照组相比,低剂量(0.3%和0.6%)组极显著降低了大肠杆菌数量(P<0.01),且以0.6%组效果最佳。与0.6%组相比,高剂量(1.5%和3.0%)组肠杆菌和大肠杆菌数量显著或极显著增加(P<0.05或P<0.01)。本试验结果提示,饲粮中添加0.3%~0.6%的硫酸钠能提高蛋鸡的抗氧化能力,改善肠道形态和调节微生物菌群,且蛋鸡饲粮中硫酸钠适宜添加水平为0.6%。

关键词： 硫酸钠; 蛋鸡; 生产性能; 抗氧化能力; 肠道形态; 微生物菌群

本文引用格式

熊平文 , 刘兵 , 陈娜娜 , 何俊娜 , 余东游 . 硫酸钠对蛋鸡生产性能、抗氧化能力、肠道形态及盲肠微生物菌群的影响[J]. 动物营养学报, 2015 , 27(11) : 3358 -3365 . DOI: 10.3969/j.issn.1006-267x.2015.11.007

Abstract

This experiment was conducted to evaluate the effects of sodium sulfate supplementation in laying hens diet by determining performance, antioxidant capacity, intestinal morphology and cecal microflora population. Four hundred and fifty 20-week-old Hyline White laying hens with similar body weight and laying rate were randomly allocated to 5 groups with 6 replicates per group and 15 hens per replicate. Hens in the control group was fed a basal diet, and hens in experimental groups were fed the basal diets supplemented with 0.3%, 0.6%, 1.5%, 3.0% sodium sulfate, respectively. The trial lasted for 8 weeks. The results showed as follows:1) diets supplemented with sodium sulfate tended to improve the laying performance of laying hens with no significant effect (P>0.05), and the effect of low dose groups (0.3% and 0.6%) was better. 2) Compared with control group, diets supplemented with sodium sulfate had no significant influences on antioxidant parameters (P>0.05). Compared with 0.6% group, the activities of glutathione peroxidase (GSH-Px) in plasma and total superoxide dismutase (T-SOD) in liver of high dose (1.5% and 3.0%) groups were significantly decreased (P<0.05 or P<0.01), group added by 3.0% had significantly reduced the total antioxidant capacity (T-AOC) in plasma and the activity of GSH-Px in liver (P<0.05). 3) Compared with control group, the villus height in duodenum and ileum of 0.6% group had significantly raised by 30.51% and 42.63% (P<0.01); the crypt depth in duodenum was significantly reduced (P<0.05) and the ratio of villus height to crypt depth (V/C) in duodenum was significantly raised (P<0.05). Compared with 0.6% group, the villus height in duodenum and ileum and V/C in duodenum of high dose (1.5% and 3.0%) groups were significantly decreased (P<0.01), and the crypt depth in duodenum and ileum of 3.0% group was significantly increased (P<0.01). 4) Compared with control group, 0.3% and 0.6% groups had remarkably reduced the number of Escherichia coli in cecum (P<0.01), and the group supplemented with 0.6% was the best; compared with 0.6% group, high dose (1.5% and 3.0%) groups had significantly increased the number of Enterobacteria and Escherichia coli in cecum (P<0.05 or P<0.01). It is concluded that dietary sodium sulfate supplemental level at 0.3% to 0.6% tend to improve the antioxidant capacity and intestinal morphology, and regulate the cecal microflora population in laying hens. The optimum supplemental level of sodium sulfate is 0.6% in laying hens diet.

Key words： sodium sulfate; laying hens; performance; antioxidant capacity; intestinal morphology; microflora population

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