本试验旨在研究饲粮中添加不同种类的酸化剂对肉鸡肠道发育、消化酶活性以及微生物数量的影响。试验选用1日龄的爱拔益加肉公鸡840只,随机分为7个处理,每个处理6个重复,每个重复20只鸡。对照组饲喂基础饲粮,其余6组饲喂在基础饲粮中分别添加吸附磷酸(PA)、微囊化磷酸(CPA)、吸附磷酸乳酸(PLA)、富马酸(FUA)、甲酸(FA)和柠檬酸(CA)的试验饲粮,氢离子(H⁺)浓度均为9.18 mmol/kg。试验期为42 d。结果表明:1)饲粮中添加PA显著提高了42日龄肉鸡小肠胰蛋白酶和糜蛋白酶活性(P<0.05)。2)饲粮中添加CPA显著增加了21和42日龄肉鸡小肠糜蛋白酶活性(P<0.05)。3)饲粮中添加PLA显著提高了42日龄肉鸡小肠胰蛋白酶活性(P<0.05)。4)饲粮中添加FUA显著增加了42日龄肉鸡小肠胰蛋白酶和糜蛋白酶活性(P<0.05),显著降低了42日龄肉鸡盲肠大肠杆菌数量(P<0.05)。5)饲粮中添加FA显著增加了21和42日龄肉鸡小肠胰蛋白酶活性(P<0.05),显著降低了42日龄肉鸡盲肠大肠杆菌数量(P<0.05)。6)饲粮中添加CA显著增加了21和42日龄肉鸡小肠胰蛋白酶和糜蛋白酶活性(P<0.05),显著降低了42日龄肉鸡盲肠大肠杆菌数量(P<0.05)。由此可见,PA、CPA和PLA均可不同程度增强肉鸡小肠消化酶活性,而FUA、FA和CA则均具有改善肉鸡肠道消化酶活性和降低有害微生物数量的功能。

To investigate the effects of different kinds of acidifier on intestinal development, digestive enzyme activities and microbiota number of broiler chickens, a total of 840 one-day-old male Arbor Acres (AA) broiler chicks were randomly allocated into 7 treatments with 6 replicates per group and 20 broilers per replicate. The control group was fed a basal diet, and test groups were fed the basal diet supplemented with phosphoric acid (PA), coated phosphoric acid (CPA), phosphoric acid+lactic acid (PLA), fumaric acid (FUA), formic acid (FA) and citric acid (CA), respectively (the H⁺ concentration was 9.18 mmol/kg). The experiment lasted for 42 days. The results showed as follows:1) dietary supplemented with PA significantly increased the activities of trypsin and chymotrypsin in small intestine at 21 and 42 days of age (P<0.05). 2) Dietary supplemented with CPA significantly increased the chymotrypsin activity in small intestine (P<0.05). 3) Dietary supplemented with PLA significantly increased trypsin activity in small intestine at 42 days of age (P<0.05). 4) Dietary supplemented with FUA significantly increased the activities of trypsin and chymotrypsin in the small intestine at 42 days of age (P<0.05), but significantly reduced the number of Escherichia coli in cecal at 42 days of age (P<0.05). 5) Dietary supplemented with FA significantly increased the trypsin activity in the small intestine at 21 and 42 days of age (P<0.05), but significantly reduced the number of Escherichia coli in cecal at 42 days of age (P<0.05). 6) Dietary supplemented with CA significantly increased the activities of trypsin and chymotrypsin in the small intestine at 21 and 42 days of age (P<0.05), but significantly reduced the number of Escherichia coli in cecal at 42 days of age (P<0.05). In conclusion, PA, CPA and PLA can increase small intestinal digestive enzyme activities; FUA, FA and CA can improve small intestinal digestive enzyme activities, and decrease harmful cecal microbiota number of broilers.