动物营养学报 >

2015 , Vol. 27 >Issue 11: 3567 - 3576

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.11.030

研究简报 Short Communications

膨化豆粕替代不同比例鱼粉对黄鳝生长性能、体成分、肠道消化酶活力及血清生化指标的影响

  • 张俊智 ,
  • 吕富 ,
  • 郇志利 ,
  • 刘庄鹏 ,
  • 胡毅 ,
  • 王萍 ,
  • 谢骏
展开
  • 1. 湖南农业大学动物科学技术学院, 长沙 410128;
    2. 水产高效健康生产湖南省协同创新中心, 常德 415000;
    3. 盐城工学院海洋与生物工程学院, 盐城 224051;
    4. 淮安新希望饲料有限公司, 淮安 223005;
    5. 农业部淡水渔业和种质资源利用重点实验室, 中国水产科学研究院淡水 渔业研究中心, 无锡 214081

收稿日期: 2015-05-12

  网络出版日期: 2015-11-21

基金资助

国家自然科学基金(31572626);苏北科技发展专项资金(BC2013429);湖南省教育厅优秀青年项目(14B089);农业部淡水渔业与种质资源利用重点实验室开放基金(FK201504)

收起

Effects of Fish Meal Replacement by Different Proportions of Extruded Soybean Meal on Growth Performance, Body Composition, Intestinal Digestive Enzyme Activities and Serum Biochemical Indices of Rice Filed Eel (Monopterus albus)

  • ZHANG Junzhi ,
  • LYU Fu ,
  • HUAN Zhili ,
  • LIU Zhuangpeng ,
  • HU Yi ,
  • WANG Ping ,
  • XIE Jun
Expand
  • 1. College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China;
    2. Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde 415000, China;
    3. Marine and Biological Engineering College, Yancheng Institute of Technology, Yancheng 224051, China;
    4. Huaian New Hope Feed Co., Ltd., Huaian 223005, China;
    5. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

Received date: 2015-05-12

  Online published: 2015-11-21

Fold

摘要

以初始体重(19.55±0.15) g的黄鳝(Monopterus albus)为研究对象,用膨化豆粕分别替代基础饲料(含55%鱼粉)中0、12%、24%、36%和48%的鱼粉,配制膨化豆粕含量分别为0(对照)、9.3%、18.6%、28.0%和37.2%的5种等氮等脂的试验饲料,研究其对黄鳝生长性能、体成分、肠道消化酶活力及血清生化指标的影响,养殖试验持续90 d。每种饲料设3个重复,每个重复饲养黄鳝100尾。结果表明:与对照组相比,当膨化豆粕含量大于18.6%时,黄鳝的增重率,机体粗脂肪含量,血清葡萄糖含量,肠道脂肪酶、淀粉酶活力及肌肉中必需氨基酸含量、必需氨基酸/总氨基酸显著下降(P<0.05),饲料系数显著升高(P<0.05);当膨化豆粕含量达到37.2%时,血清尿素氮含量显著升高(P<0.05)。随着饲料中膨化豆粕含量的升高, 肠道胰蛋白酶活力呈先上升后下降的趋势,血清谷草转氨酶活力呈下降趋势,而血清谷丙转氨酶活力呈上升趋势。综上所述,以膨化豆粕替代鱼粉,黄鳝配合饲料中膨化豆粕的含量以不超过18.6%为宜。

关键词： 黄鳝; 膨化豆粕; 生长性能; 消化酶; 体成分; 血清生化指标

本文引用格式

张俊智 , 吕富 , 郇志利 , 刘庄鹏 , 胡毅 , 王萍 , 谢骏 . 膨化豆粕替代不同比例鱼粉对黄鳝生长性能、体成分、肠道消化酶活力及血清生化指标的影响[J]. 动物营养学报, 2015 , 27(11) : 3567 -3576 . DOI: 10.3969/j.issn.1006-267x.2015.11.030

Abstract

A 90-day feeding trial was conducted to investigate the effects of fish meal (FM) replacement by different proportions of extruded soybean meal on growth performance, body composition, intestinal digestive enzyme activities and serum biochemical indices of rice filed eels (Monopterus albus)[initial average body weight of (19.55±0.15) g]. Five isonitrogenous and isolipidic diets were formulated to contain 0 (control), 9.3%, 18.6%, 28.0%, 37.2% extruded soybean meal which replaced 0, 12%, 24%, 36% and 48% FM in the basal diet (contained 55% FM), respectively. Each diet had three replicates, and each replicate had 100 rice filed eels. Results showed as follows:compared with control group, the weight gain rate, body crude lipid content, serum glucose content, intestinal lipase and amylase activities, and essential amino acid content and essential amino acid/total amino acid in muscle were significantly reduced (P<0.05), but feed conversion rate was significantly increased (P<0.05); when dietary extruded soybean meal content was more than 18.6%, the content of serum urea nitrogen was significantly reduced when dietary extruded soybean meal content arrived at 37.2% (P<0.05). With the dietary extruded soybean meal content increasing, the intestinal trypsin activity was firstly increased and then decreased, but the serum glutamic oxaloacetic transaminase activity showed an decrease trend and the glutamic-pyruvic transaminase activity showed an increase trend. In conclusion, extruded soybean meal in the diet of rice field eels is not more than 18.6% when FM replacement by extruded soybean meal.

Key words： rice field eel (Monopterus albus); extruded soybean meal; growth performance; digestive enzymes; body composition; serum biochemical indices

参考文献

[1] FAO.The state of world fisheries and aquaculture[R].Rome:Food and Agriculture Organization of the United Nations, 2009.
[2] TANTIKITTI C, SANGPONG W, CHIAVAREESAJJA S.Effects of defatted soybean protein levels on growth performance and nitrogen and phosphorus excretion in Asian seabass (Lates calcarifer)[J].Aquaculture, 2005, 248(1/2/3/4):41-50.
[3] REFSTIE S, STOREBAKKEN T, ROEM A J.Feed consumption and conversion in Atlantic salmon (Salmo salar) fed diets with fish meal, extracted soybean meal or soybean meal with reduced content of oligosaccharides, trypsin inhibitors, lectins and soya antigens[J].Aquaculture, 1998, 162(3/4):301-312.
[4] BAKKE-MCKELLEP A M, PRESS C M, BAEVERFJORD G, et al.Changes in immune and enzyme histochemical phenotypes of cells in the intestinal mucosa of Atlantic salmon, Salmo salar L., with soybean meal-induced enteritis[J].Journal of Fish Diseases, 2000, 23(2):115-127.  
[5] LIN S M, MAI K S, TAN B P.Effect of soybean meal replacement by rapeseed-cottonseed compound on growth, body composition and immunity of tilapia Oreochromis niloticus×O. aureus[J].Oceanologia Et Limnologia Sinica, 2007, 38(2):168-173.
[6] OPAPEJU F O, GOLIAN A, NYACHOTI C M, et al.Animo acid digestibility in dry extruded-expelled soybean meal fed to pigs and poultry[J].Journal of Animal Science, 2006, 84(5):1130-1137.
[7] 杨代勤, 陈芳, 李道霞, 等.黄鳝的营养素需要量及饲料最适能量蛋白比[J].水产学报, 2000, 24(3):259-262.
[8] 杨代勤, 陈芳, 阮国良.饲料中添加胆碱对黄鳝生长、组织脂肪含量及消化酶活性的影响[J].水产学报, 2006, 30(5):676-682.
[9] 张国辉, 何瑞国, 张世萍, 等.维生素E对黄鳝繁殖性能的影响[J].水生生物学报, 2007, 31(2):196-200.
[10] 曹志华, 罗静波, 文华, 等.肉骨粉代替不同水平的鱼粉对黄鳝生长的影响[J].湖北农业科学, 2009, 48(8):1971-1972.
[11] AOAC.Official methods of analysis of AOAC international[M].16th ed.Arlington, VA:AOAC International, 1995.
[12] 陈爱莲, 苏娅.利用微软EXCEL计算必需氨基酸指数[J].粮食与饲料工业, 1998(10):29-30.
[13] 向枭, 周兴华, 陈建, 等.饲料中豆粕蛋白替代鱼粉蛋白对齐口裂腹鱼幼鱼生长性能、体成分及血液生化指标的影响[J].水产学报, 2012, 36(5):723-731.
[14] KOKOU F, RIGOS G, HENRY M, et al.Growth performance, feed utilization and non-specific immune response of gilthead sea bream (Sparus aurata L.) fed graded levels of a bioprocessed soybean meal[J].Aquaculture, 2012, 364-365:74-81.
[15] 陆阳, 杨雨虹, 王裕玉, 等.不同比例膨化豆粕替代鱼粉对虹鳟生长、体成分及血液学指标的影响[J].动物营养学报, 2010, 22(1):221-227.
[16] 高荣兵, 庄平, 章龙珍, 等.豆粕替代鱼粉对点篮子鱼生长性能的影响[J].水产学报, 2010, 34(10):1534-1540.
[17] 张帆, 张文兵, 麦康森, 等.饲料中豆粕替代鱼粉对大黄鱼生长、消化酶活性和消化道组织学的影响[J].中国海洋大学学报, 2012, 42(增刊):75-82.
[18] BISWAS A, BISWAS B K, ITO J, et al.Soybean meal can partially replace enzyme-treated fish meal in the diet of juvenile pacific bluefin tuna Thunnus orientalis[J].Fisheries Science, 2011, 77(4):615-621.  
[19] 杨代勤, 严安生, 陈芳.几种氨基酸及香味物质对黄鳝诱食活性的初步研究[J].水生生物学报, 2002, 26(2):205-208.
[20] 艾庆辉, 谢小军.南方鲇的营养学研究:饲料中大豆蛋白水平对生长的影响[J].水生生物学报, 2002, 26(1):57-65.
[21] 苗雪霞, 陈红歌, 李秀兰, 等.植酸酶对肉鸡生长及磷利用率的影响[J].河南农业大学学报, 1999, 33(1):68-71.
[22] CHENG Z J, HARDY R W, USRY J L.Effects of lysine supplementation in plant protein-based diets on the performance of rainbow trout (Oncorhynchus mykiss) and apparent digestibility coefficients of nutrients[J].Aquaculture, 2003, 215(1/2/3/4):255-265.
[23] EL-SAIDY D M S D, GABER M M A.Complete replacement of fish meal by soy bean meal with dietary L-lysine supplementation for Nile tilapia Oreochromis niloticus (L.) fingerlings[J].Journal of the World Aquaculture Social, 2002, 33(3):297-306.  
[24] 马晓.肉骨粉替代鱼粉对黄鳝(Monopterus albus)生长性能及营养成分的影响[D].硕士学位论文.长沙:湖南农业大学, 2011:45-50.
[25] 吴莉芳, 安丽影, 秦贵信, 等.不同大豆蛋白源对埃及胡子鲇生长、饲料利用及体成分的影响[J].水生生物学报, 2009, 33(6), 1214-1218.
[26] 王桂芹, 周洪旗, 陈建明, 等.翘嘴红鲌对饲料蛋白的营养需求及豆粕对鱼粉的适宜替代量[J].中国水产科学, 2006, 13(2):277-285.
[27] DAY O J, PLASCENCIA GONZÁLEZ H G.Soybean protein concentrate as a protein source for turbot Scophthalmus maximus L.[J].Aquaculture Nutrition, 2000, 6(4):221-228.  
[28] DAVIES S J, MORRIS P C.Influence of multiple amino acid supplementation on the performance of rainbow trout, Oncorhynchus mykiss (Walbaum), fed soya based diets[J].Aquaculture Research, 1997, 28(1):65-74.  
[29] REFSTIE S, STOREBAKKEN T, BAEVERFJORD G, et al.Long-term protein and lipid growth of Atlantic salmon (Salmo salar) fed diets with partial replacement of fish meal by soy protein products at medium or high lipid level[J].Aquaculture, 2001, 193(1/2):91-106.
[30] 贾艳菊, 杨振才.膨化饲料动植物蛋白比对中华鳖稚鳖生长特性的影响[J].水生生物学报, 2007, 31(4):570-575.
[31] ELANGOVAN A, SHIM K F.The influence of replacing fish meal partially in the diet with soybean meal on growth and body com position of juvenile tin foil barb (Barbodes altus)[J].Aquaculture, 2000, 189(1/2):133-144.
[32] LEENHOUWERS J I, ADJEI-BOATENG D, VERRETH J A J, et al.Digesta viscosity, nutrient digestibility and organ weights in African catfish (Clarias gariepinus) fed diets supplemented with different levels of a soluble non-starch polysaccharide[J].Aquaculture Nutrition, 2006, 12(2):111-116.  
[33] 李正忠.花粉、灵芝与珍珠中必需氨基酸的定量测定与分析比较[J].氨基酸杂志, 1988(4):41-43.
[34] LIN S M, LUO L.Effects of different levels of soybean meal inclusion in replacement for fish meal on growth, digestive enzymes and transaminase activities in practical diets for juvenile tilapia, Oreochromis niloticus×O. aureus[J].Animal Feed Science and Technology, 2011, 168(1/2):80-87.
[35] 张璐, 艾庆辉, 麦康森, 等.植酸酶和非淀粉多糖酶对鲈鱼生长和消化酶活性的影响[J].水生生物学报, 2009, 33(1):82-88.
[36] YU D H, GONG S Y, YUAN Y C, et al.Effects of replacing fish meal with soybean meal on growth, body composition and digestive enzyme activities of juvenile Chinese sucker, Myxocyprinus asiaticus[J].Aquaculture Nutrition, 2013, 19(1):84-90.  
[37] DENSTADLI V, SKREDE A, KROGDAHL A, et al.Feed intake, growth, feed conversion, digestibility, enzyme activities and intestinal structure in Atlantic salmon (Salmo salar L.) fed graded levels of phytic acid[J].Aquaculture, 2006, 256(1/2/3/4):365-376.
[38] 沈同, 王镜岩.生物化学[M].2版.北京:高等教育出版社, 1996:193-201.
[39] KAUSHIK S J, CRAVEDI J P, LALLES J P, et al.Partial or total replacement of fish meal by soybean protein on growth, protein utilization, potential estrogenic or antigenic effects, cholesterolemia and flesh quality in rainbow trout (Oncorhynchus mykiss)[J].Aquaculture, 1995, 133(3/4):257-274.
[40] SETCHELL K D R, CASSIDY A.Dietary isoflavones:biological effects and relevance to human health[J].The Journal of Nutrition, 1999, 129(3):758S-767S.
[41] DENG J M, MAI K S, AI Q H, et al.Effects of replacing fish meal with soy protein concentrate on feed intake and growth of juvenile Japanese ounder, Paralichthys olivaceus[J].Aquaculture, 2006, 258(1/2/3/4):503-513.
[42] 刘襄河, 叶继丹, 王子甲, 等.饲料中豆粕替代鱼粉比例对牙鲆生长性能及生化指标的影响[J].水产学报, 2010, 34(3):450-458.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2