水产营养 Aquaculture Nutrition

饲料蛋白质水平对黄姑鱼幼鱼生长性能、体组成和消化酶活性的影响

  • 鲁琼 ,
  • 王立改 ,
  • 楼宝 ,
  • 詹炜 ,
  • 陈睿毅 ,
  • 罗胜玉 ,
  • 刘佳节 ,
  • 王肇霖
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  • 1. 浙江海洋学院, 舟山 316022;
    2. 浙江省海洋水产研究所, 舟山 316021;
    3. 浙江省海水增养殖重点实验室, 舟山 316021

收稿日期: 2015-06-08

  网络出版日期: 2015-12-14

基金资助

浙江省科技计划项目(2012C12907-8,2013F20001);浙江海洋学院科研启动经费资助

Effects of Dietary Protein Level on Growth Performance, Body Composition and Digestive Enzyme Activities of Juvenile Nibea albiflora

  • LU Qiong ,
  • WANG Ligai ,
  • LOU Bao ,
  • ZHAN Wei ,
  • CHEN Ruiyi ,
  • LUO Shengyu ,
  • LIU Jiajie ,
  • WANG Zhaolin
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  • 1. Zhejiang Ocean University, Zhoushan 316022, China;
    2. Marine Fisheries Research Institute of Zhejiang Province, Zhoushan 316021, China;
    3. Key Lab of Mariculture and Enhancement of Zhejiang Province, Zhoushan 316021, China

Received date: 2015-06-08

  Online published: 2015-12-14

摘要

本试验旨在研究饲料蛋白质水平对黄姑鱼幼鱼生长性能、体组成和消化酶活性的影响。试验以鱼粉、豆粕和小麦蛋白粉为蛋白质源,配制蛋白质水平实测值分别为37.52%、41.80%、46.52%、49.84%、56.80%和61.48%的6种等能等脂饲料。试验选取平均体重为(17.84±0.34) g的黄姑鱼幼鱼360尾,随机分成6组,每组3个重复,每个重复20尾鱼,饲养于500 L流水钢化玻璃水桶中,在水温(27±2)℃、盐度28~29的条件下进行8周的生长试验。结果表明:饲料蛋白质水平为56.80%时,黄姑鱼幼鱼的增重率、特定生长率和饲料转化率显著高于除61.48%组以外的其余各组(P<0.05)。随着饲料蛋白质水平的升高,黄姑鱼幼鱼的肝体比、脏体比和肥满度的变化没有显著差异(P>0.05)。黄姑鱼幼鱼全鱼粗蛋白质、粗脂肪和粗灰分含量不受饲料蛋白质水平的显著影响(P>0.05)。黄姑鱼幼鱼肠道蛋白酶活性随饲料蛋白质水平的升高先升高后稳定,饲料蛋白质水平对黄姑鱼幼鱼肠道淀粉酶、脂肪酶和肝脏蛋白酶、淀粉酶、脂肪酶的活性均无显著影响(P>0.05)。以特定生长率为评价指标,经折线模型分析表明,黄姑鱼幼鱼对饲料中蛋白质的最适需求量为55.39%。

本文引用格式

鲁琼 , 王立改 , 楼宝 , 詹炜 , 陈睿毅 , 罗胜玉 , 刘佳节 , 王肇霖 . 饲料蛋白质水平对黄姑鱼幼鱼生长性能、体组成和消化酶活性的影响[J]. 动物营养学报, 2015 , 27(12) : 3763 -3771 . DOI: 10.3969/j.issn.1006-267x.2015.12.015

Abstract

This experiment was conducted to investigate the effects of dietary protein level on growth performance, body composition and digestive enzyme activities of juvenile Nibea albiflora. Fish meal, soybean meal and wheat gluten meal were used as main dietary protein sources to formulate isoenergetic and isolipidic diets at six protein levels, which were 37.52%, 41.80%, 46.52%, 49.84%, 56.80% and 61.48%, respectively. A total of 360 juvenile Nibea albiflora with the average body weight of(17.84±0.34) g were randomly allocated into 6 groups with 3 replicates per group and 20 juvenile Nibea albiflora per replicate, and they were reared in 500 L water-flowing glass fiber reinforced plastic buckets, separately. An 8-week growth experiment was conducted under the condition of water temperature(27±2)℃ and salinity 28 to 29. The results showed that the weight gain rate, specific growth rate and feed conversion rate of the juvenile Nibea albiflora in the 56.80% group were significantly higher than those in the other groups except 61.48% group(P<0.05). With the dietary protein level increasing, there were no significant changes in hepatosomatic index, viscerasomatic index and condition factor of juvenile Nibea albiflora. Dietary protein level had no significant influences on the contents of crude protein, crude lipid and ash in the body of juvenile Nibea albiflora(P>0.05). The intestinal protease activity of juvenile Nibea albiflora increased at first and then decreased with the dietary protein level increasing. Dietary protein level had no significant influences on the activities of intestinal amylase, lipase as well as protease, amylase and lipase in the liver(P>0.05). With specific growth rate as an evaluating index, the optimal dietary protein requirement estimated by broken-line model is 55.39% for juvenile Nibea albiflora.

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