本试验旨在研究饲粮L-精氨酸水平对育成期水貂氨基酸消化率、血清氨基酸含量及血清生化指标的影响。选取(60±3)日龄健康雄性水貂70只,随机分为7组,每组10个重复,每个重复为1只水貂。各组分别饲喂在基础饲粮中添加0(Ⅰ组)、0.20%(Ⅱ组)、0.40%(Ⅲ组)、0.60%(Ⅳ组)、0.80%(Ⅴ组)、1.00%(Ⅵ组)和1.20%(Ⅶ组)L-精氨酸的试验饲粮。预试期7 d,正试期60 d。结果表明:1)Ⅱ组水貂的丝氨酸、谷氨酸、脯氨酸、甘氨酸、丙氨酸、缬氨酸、异亮氨酸、酪氨酸、赖氨酸消化率显著或极显著高于组Ⅲ、Ⅳ、Ⅴ、Ⅵ和Ⅶ组(P<0.05或P<0.01);Ⅱ组水貂的天冬氨酸、苏氨酸、亮氨酸、苯丙氨酸消化率显著或极显著高于Ⅲ和Ⅳ组(P<0.05或P<0.01)。2)Ⅵ和Ⅶ组水貂的血清精氨酸含量显著高于Ⅰ、Ⅱ和Ⅲ组(P<0.05);水貂血清其他氨基酸含量各组间差异不显著(P>0.05)。3)Ⅳ组水貂血清总蛋白含量极显著高于Ⅴ和Ⅶ组(P<0.01),显著高于Ⅰ和Ⅵ组(P<0.05);Ⅲ和Ⅳ组水貂血清白蛋白含量极显著高于Ⅰ、Ⅴ、Ⅵ和Ⅶ组(P<0.01);Ⅳ组水貂血清球蛋白含量显著高于Ⅰ、Ⅴ和Ⅶ组(P<0.05);Ⅳ组水貂血清碱性磷酸酶活性显著高于Ⅰ、Ⅱ、Ⅴ和Ⅶ组(P<0.05);Ⅳ组水貂血清乳酸脱氢酶活性显著高于Ⅰ、Ⅱ、Ⅴ和Ⅶ组(P<0.05);各组水貂血清丙氨酸氨基转移酶和天门冬氨酸氨基转移酶活性差异不显著(P>0.05)。4)Ⅲ组水貂血清免疫球蛋白M含量显著高于Ⅰ组(P<0.05);Ⅱ、Ⅲ和Ⅳ组水貂血清补体3含量显著高于Ⅰ、Ⅴ、Ⅵ、Ⅶ组(P<0.05);各组水貂血清免疫球蛋白A、免疫球蛋白G和补体4含量差异不显著(P>0.05)。通过本试验的研究和回归分析发现,饲粮L-精氨酸添加水平为0.20%~0.41%(饲粮精氨酸总水平为1.65%~1.86%)时,育成期水貂能获得较好的氨基酸消化率和血清生化指标。

This study was conducted to study the effects of dietary L-arginine(L-Arg) level on amino acid digestibility, serum amino acid content and serum biochemical indices of growing minks. Seventy healthy female mink at the age of 60 days were randomly divided into 7 groups with 10 replicates per group and 1 mink per replicate. The mink in 7 groups were fed experimental diets with L-Arg supplementation levels of 0(group Ⅰ), 0.20%(group Ⅱ), 0.40%(group Ⅲ), 0.60%(group Ⅳ), 0.80%(group Ⅴ), 1.00%(group Ⅵ) and 1.20%(group Ⅶ), respectively. The pre-test period lasted for 7 days and the trial lasted for 60 days. The results showed as follows:1) the digestibility of serine, glutamic acid, proline, glycine, alanine, valine, isoleucine, tyrosine and lysine in group Ⅱ was significantly higher than that in groups Ⅲ, Ⅳ, Ⅴ, Ⅵ and Ⅶ(P<0.05 or P<0.01). The digestibility of aspartic acid, threonine, leucine and phenylalanine digestibility in groups Ⅱ was significantly higher than that in groups Ⅲ and Ⅳ(P<0.05 or P<0.01). 2) The serum arginine content in groups Ⅵ and Ⅶ was significantly higher than that in groups Ⅰ, Ⅱ and Ⅲ(P<0.05). There were no significantly differences in the other serum amino acid contents among all groups(P>0.05). 3) The serum total protein content in groups Ⅳ was greatly significantly higher than that in groups Ⅴ and Ⅶ(P<0.01), and was significantly higher than that in groups Ⅰ and Ⅵ(P<0.05). The serum albumin content in group Ⅲ and Ⅳ was greatly significantly higher than that in groups Ⅰ, Ⅴ, Ⅵ and Ⅶ(P<0.01). The serum globulin content in group Ⅳ was significantly higher than that in groups Ⅰ, Ⅴ and Ⅶ(P<0.05). The serum alkaline phosphatase activity in group Ⅳ was significantly higher than that in groups Ⅰ, Ⅱ, Ⅴ and Ⅶ(P<0.05). The serum lactic dehydrogenase activity in group Ⅳ was significantly higher than that in groups Ⅰ, Ⅱ, Ⅴ and Ⅶ(P<0.05). There were no significantly differences in serum alanine aminotransferase and aspartate aminotransferase activities among all groups(P>0.05). 4) The serum immunoglobulin M content in group Ⅲ was significantly higher than that in group Ⅰ(P<0.05). The serum complement 3 content in groups Ⅱ, Ⅲ and Ⅳ was significantly higher than that in groups Ⅰ, Ⅴ, Ⅵ and Ⅶ(P<0.05). There were no significantly differences in serum immunoglobulin A, immunoglobulin G and complement 4 contents among all groups(P>0.05). Through this trial study and regression analysis, when dietary L-Arg supplement level is 0.20% to 0.41%(dietary L-Arg total level is 1.65% to 1.86%), the growing minks can get better amino acid digestibility and serum biochemical indices.