抗菌肽对仔猪血清生化指标、肠黏膜形态结构及空肠上皮紧密连接蛋白基因相对表达量的影响

宦海琳; 白建勇; 周维仁; 闫俊书; 徐小明; 杨瑶; 温超; 周岩民

doi:10.3969/j.issn.1006-267x.2015.12.019

动物营养学报 >

2015 , Vol. 27 >Issue 12: 3797 - 3804

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.12.019

饲料营养 FEED SCIENCE AND TECHNOLOGY

抗菌肽对仔猪血清生化指标、肠黏膜形态结构及空肠上皮紧密连接蛋白基因相对表达量的影响

宦海琳 ,
白建勇 ,
周维仁 ,
闫俊书 ,
徐小明 ,
杨瑶 ,
温超 ,
周岩民

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1. 江苏省农业科学院畜牧研究所, 南京 210014;
2. 南京农业大学动物科技学院, 南京 210095;
3. 南京师范大学金陵女子学院, 南京 210097

收稿日期: 2015-06-25

网络出版日期: 2015-12-14

基金资助

江苏省农业自主创新基金[CX(13)5040];江苏省高校自然科学基金(13KJB550014)

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Effects of Antimicrobial Peptides on Serum Biochemical Indices, Intestinal Mucosa Morphology and the Relative Expression Level of Tight Junction Protein Gene of Jejunum of Piglets

HUAN Hailin ,
BAI Jianyong ,
ZHOU Weiren ,
YAN Junshu ,
Xu Xiaoming ,
YANG Yao ,
WEN Chao ,
ZHOU Yanmin

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1. The Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
3. Jinling College, Nanjing Normal University, Nanjing 210097, China

Received date: 2015-06-25

Online published: 2015-12-14

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摘要

本试验旨在研究饲粮中添加抗菌肽对仔猪血清生化指标、肠黏膜结构及空肠上皮紧密连接蛋白基因相对表达量的影响。选用108头35日龄、体重13 kg左右的苏钟猪随机分为3组,每组3重复,每重复12头,分别饲喂基础饲粮(对照组)、基础饲粮+40 mg/kg杆菌肽锌+20 mg/kg硫酸黏杆菌素(抗生素组)和基础饲粮+300 mg/kg抗菌肽(抗菌肽组)的试验饲粮,预试期7 d,正试期49 d。结果表明:1)与对照组相比,饲粮中添加抗菌肽能够显著提高仔猪血清总蛋白含量和谷丙转氨酶活性(P<0.05);抗菌肽组血清总蛋白含量比抗生素组极显著提高(P<0.01)。2)与对照组相比,饲粮中添加抗菌肽能够在一定程度上提高仔猪十二指肠、空肠和回肠绒毛高度,但均未达到显著水平(P>0.05);抗菌肽组仔猪空肠隐窝深度比对照组显著降低(P<0.05);抗菌肽组和抗生素组仔猪十二指肠绒毛高度与隐窝深度的比值均比对照组显著提高(P<0.05)。3)抗菌肽组仔猪空肠紧密连接蛋白闭合蛋白-1(Claudin-1)基因相对表达量比对照组显著提高(P<0.05),咬合蛋白(Occludin)和闭合小环蛋白-1(ZO-1)基因相对表达量分别比对照组提高了34.83%、8.57%(P>0.05);抗菌肽组Claudin-1基因相对表达量比抗生素组提高了32.52%,ZO-1基因相对表达量比抗生素组降低了5.00%,但均未达到显著水平(P>0.05)。综上所述,本试验条件下,饲粮中添加300 mg/kg的抗菌肽能够显著提高仔猪血清总蛋白含量和谷丙转氨酶活性;能够有效改善仔猪肠黏膜形态结构;提高仔猪空肠上皮紧密连接蛋白的基因相对表达量。

关键词： 抗菌肽; 仔猪; 肠黏膜形态结构; 紧密连接蛋白

本文引用格式

宦海琳 , 白建勇 , 周维仁 , 闫俊书 , 徐小明 , 杨瑶 , 温超 , 周岩民 . 抗菌肽对仔猪血清生化指标、肠黏膜形态结构及空肠上皮紧密连接蛋白基因相对表达量的影响[J]. 动物营养学报, 2015 , 27(12) : 3797 -3804 . DOI: 10.3969/j.issn.1006-267x.2015.12.019

Abstract

This study investigated the effects of dietary antimicrobial peptides(AMPs) on serum biochemical indices, intestinal mucosa morphology and the relative expression level of tight junction protein gene of jejunum in piglets. One hundred and eight 35-day-old piglets with similar average body weight(about 13 kg) were randomly divided into three groups with three replicates per group and twelve piglets per replicate. The piglets in three groups were fed a basal diet(control group), basal diet+40 mg/kg bacitracin zinc+20 mg/kg colistin sulfate(antibiotic group) and basal diet+300 mg/kg antimicrobial peptides(AMPs group), respectively. The pre-test period lasted 7 days, and test period lasted for 49 days. The results showed as follows:1) compared with the control group, dietary AMPs could significantly improve the content of total protein and the activity of alanine transaminase(ALT) in serum of piglets(P<0.05); the content of serum total protein in AMPs group was significantly higher than that in the antibiotic group(P<0.01). 2) Compared with the control group, AMPs could improve the villus height of duodenum, jejunum and ileum to some extent, but there was no significant difference(P>0.05); the crypt depth of jejunum in AMPs group was significantly lower than that in the control group(P<0.05); the villus height/crypt depth of duodenum in AMPs group and antibiotic group was significantly higher than that in the control group(P<0.05). 3) The relative expression level of Claudin-1 gene in AMPs group was significantly higher than that in the control group(P<0.05), and the relative expression level of Occludin and zonula occluden-1(ZO-1) genes were improved by 34.83% and 8.57% in jejunum of piglets(P>0.05), respectively; compared with the antibiotic group, the relative expression level of Claudin-1 gene was improved by 32.52%, but the relative expression level of ZO-1 gene was decreased by 5.00% in AMPs group(P>0.05). In conclusion, diet supplementation of 300 mg/kg antimicrobial peptides can significantly improve the content of total protein and the activity of ALT in serum of piglets, effectively improve the intestinal mucosa morphology, and increase the relative expression level of tight junction protein gene of jejunum in piglets under the condition of this test.

Key words： antimicrobial peptides; piglets; intestinal mucosal morphology; tight junction protein

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