早期断奶仔猪血浆氨基酸代谢谱动态变化研究

肖英平; 陈安国; 代兵; 陈镜刚; 李开锋; 杨华

doi:10.3969/j.issn.1006-267x.2015.12.025

动物营养学报 >

2015 , Vol. 27 >Issue 12: 3846 - 3853

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.12.025

研究简报 Short Communications

早期断奶仔猪血浆氨基酸代谢谱动态变化研究

肖英平 ,
陈安国 ,
代兵 ,
陈镜刚 ,
李开锋 ,
杨华

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1. 浙江农业科学院农产品质量标准研究所, 杭州 310021;
2. 浙江大学动物科学学院, 杭州 310058;
3. 浙江农林大学动物科技学院, 杭州 311300

收稿日期: 2015-06-25

网络出版日期: 2015-12-14

基金资助

国家自然科学基金(31402083);杭州市农业科研攻关专项(20140432B77)

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Dynamic Change of Plasma Amino Acid Profiles in Early Weaned Piglets

XIAO Yingping ,
CHEN Anguo ,
DAI Bing ,
CHEN Jinggang ,
LI Kaifeng ,
YANG Hua

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1. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
2. College of Animal Sciences, Zhejiang University, Hangzhou 310058, China;
3. College of Animal Science & Technology, Zhejiang A & F University, Hangzhou 311300, China

Received date: 2015-06-25

Online published: 2015-12-14

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摘要

本试验旨在研究仔猪早期断奶过程中血浆氨基酸代谢谱的动态变化。选取8头21日龄"杜×长×大"断奶仔猪,在断奶后0、1、3、5、7、10、15、30 d采集血液样本用于氨基酸分析。结果表明,仔猪血浆精氨酸、瓜氨酸和鸟氨酸含量在断奶后1~5 d内显著下降(P<0.05),在断奶后7~30 d上升。亮氨酸、脯氨酸、酪氨酸和牛磺酸含量在断奶后1~3 d内显著下降(P<0.05),然后上升。异亮氨酸、赖氨酸、甘氨酸和丝氨酸含量在断奶后5 d显著低于其他时间点的含量(P<0.05)。组氨酸、苯丙氨酸、缬氨酸、天冬氨酸、半胱氨酸含量在断奶后10 d最低。谷氨酸含量在断奶后0、1和10 d显著低于其他时间点含量(P<0.05)。总氨基酸含量在断奶后1~5 d逐渐降低(P<0.05),断奶后7 d起升高并至断奶后30 d无显著变化(P>0.05)。主成分分析显示,随着断奶后时间的延长,仔猪血浆氨基酸代谢谱与断奶后0 d的代谢谱差异逐渐变大,然后逐渐向断奶后0 d代谢谱位置靠拢,并趋于稳定,其中断后3~5 d血浆氨基酸代谢谱变化幅度最大。偏最小二乘法判别分析模型表明,谷氨酸在NRC(1998)饲养标准下区分断奶后各时间点血浆代谢谱最重要的氨基酸。由此可见,仔猪在早期断奶阶段机体的蛋白质或者氨基酸代谢发生明显变化,且在断奶后3~5 d变化幅度最为显著。

关键词： 仔猪; 早期断奶; 血浆; 氨基酸; 代谢谱

本文引用格式

肖英平 , 陈安国 , 代兵 , 陈镜刚 , 李开锋 , 杨华 . 早期断奶仔猪血浆氨基酸代谢谱动态变化研究[J]. 动物营养学报, 2015 , 27(12) : 3846 -3853 . DOI: 10.3969/j.issn.1006-267x.2015.12.025

Abstract

The time-course of plasma amino acid(AA) following response to early weaning was investigated over a 30 d period in weaned piglets using AA analyzer. Eight 21-day-old "Duroc×Landrace×Large Yorkshire" weaned piglets were selected and their blood samples were collected on days 0, 1, 3, 5, 7, 10, 15, and 30 post-weaning. The results showed as follows:plasma contents of arginine and its immediate precursors(citrulline and ornithine) decreased(P<0.05) from days 0 to 5 post-weaning, and increased from days 7 to 30 post-weaning. Plasma contents of leucine, proline, tyrosine and taurine decreased(P<0.05) on days 1 and 3 post-weaning, and increased to the level of day 0 post-weaning on day 30. Plasma contents of isoleucine, lysine, glycine and serine were the lowest on day 5 post-weaning; plasma contents of histidine, phenylalanine, valine, aspartic acid, cysteine were the lowest on day 10 post-weaning, which then increased thereafter. Plasma content of glutamic acid was significantly higher on days 0, 1, 10 post-weaning than that of the others(P<0.05). Total AA content was significantly decreased from day 1 to day 5 post-weaning(P<0.05), then increased on day 7 and keep constant thereafter with no significant difference to day 30 post-weaning(P>0.05). In score plot of principal component analysis, the profiles of AA were gradually away from the profile of AA on day 0 post-weaning, and then were closed to that, and tended to be stable. On days 3 and 5 post-weaning, the profiles of AA were robustly altered. The pattern of partial least-square discriminant analysis demonstrated that glutamic acid played the most important role in differentiating the profiles of AA at different time points post-weaning. It is concluded that early weaning changes the metabolism of protein and amino acid in the body of piglets, especially on days 3 and 5 post-weaning.

Key words： piglets; early weaning; plasma; amino acid; metabolic profiles

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