饥饿银鲳幼鱼的能量利用规律及其生存策略

闫雪梅; 谷江稳; 郭晓鸽; 巩建华; 徐善良

doi:10.3969/j.issn.1006-267x.2015.12.040

动物营养学报 >

2015 , Vol. 27 >Issue 12: 3975 - 3983

DOI: https://doi.org/10.3969/j.issn.1006-267x.2015.12.040

研究简报 Short Communications

饥饿银鲳幼鱼的能量利用规律及其生存策略

闫雪梅 ,
谷江稳 ,
郭晓鸽 ,
巩建华 ,
徐善良

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宁波大学海洋学院, 宁波 315211

收稿日期: 2015-06-04

网络出版日期: 2015-12-14

基金资助

宁波市科技重大专项(2015C110003);国家星火计划项目(2013GA701045)

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Energy Utilization Pattern and Survival Strategy in Starvation Juvenile Pampus argenteus

YAN Xuemei ,
GU Jiangwen ,
GUO Xiaoge ,
GONG Jianhua ,
XU Shanliang

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School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2015-06-04

Online published: 2015-12-14

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摘要

为了探究银鲳(Pampus argenteus)幼鱼在饥饿胁迫下能量的利用规律,分析测定了体重(2.61±1.05) g的银鲳幼鱼分别在饥饿处理0(S0组)、3(S3组)、5(S5组)、7(S7组)、9 d(S9组)后全鱼(去内脏)的粗蛋白质、粗脂肪、粗灰分等生化成分的含量及肝脏和胃肠道中的胃蛋白酶、胰蛋白酶、脂肪酶和淀粉酶的活性。结果表明:1)鱼体粗蛋白质含量在饥饿期间显著下降(P<0.05),饥饿9 d时粗蛋白质累计损失率达到15.23%,粗蛋白质最大降幅出现在饥饿7 d时,达5.51%,此时肝脏及胃肠道中的胃蛋白酶活性也显著上升(P<0.05)。2)鱼体粗脂肪含量在饥饿前期下降较快,饥饿5 d时损失率即高达77.86%,此时肝脏及胃肠道中的脂肪酶活性也达到最高点,分别是S0组的1.5和1.4倍(P<0.05)。此后粗脂肪消耗显著减少(P<0.05),脂肪酶活性也骤然下跌。3)随鱼体粗蛋白质和粗脂肪含量的下降,鱼体粗灰分含量则持续增加,饥饿3 d时较S0组增幅达1.7倍(P<0.05)。4)无氮浸出物在饥饿前期下降不显著(P>0.05),至饥饿9 d时骤降至0.61%,相对损失率达28.15%(P<0.05);此时,肝脏中的淀粉酶活性也由S0组的0.31 U/mg升至0.69 U/mg,达到峰值。5)鱼体比能值变化印证了饥饿银鲳幼鱼对能量的利用规律。由此可见,银鲳幼鱼在饥饿胁迫下的生存策略表现为:初期主要利用脂肪提供能量,随着饥饿时间延长,开始动用蛋白质作为主要能源物质,饥饿后期随着脂肪与蛋白质利用接近极限,只能加大对碳水化合物的利用。

关键词： 银鲳; 饥饿胁迫; 生化成分; 消化酶活性; 生存策略

本文引用格式

闫雪梅 , 谷江稳 , 郭晓鸽 , 巩建华 , 徐善良 . 饥饿银鲳幼鱼的能量利用规律及其生存策略[J]. 动物营养学报, 2015 , 27(12) : 3975 -3983 . DOI: 10.3969/j.issn.1006-267x.2015.12.040

Abstract

To reveal the energy utilization pattern of juvenile Pampus argenteus on starvation stress, the biochemical components such as crude protein, crude fat, ash contents of whole fish with gutted and the activities of pepsin, trypsin, lipase and amylase in liver and gastrointestinal tract were analyzed by using the juvenile P. argenteus[body weight:(2.61±1.05) g] in hunger 0(S0 group), 3(S3 group), 5(S5 group), 7(S7 group) and 9 days(S9 group), respectively. The results showed as follows:1) the crude protein content significantly decreased during starvation(P<0.05), the cumulative loss rate of crude protein was up to 15.23% in hunger 9 days, and the biggest loss rate appeared in the 7th day which was up to 5.51%. At that moment, pepsin activity in liver and gastrointestinal tract significantly increased(P<0.05). 2) The crude fat content decreased rapidly in earlier starvation period, the loss rate of crude fat was up to 77.86% in hunger 5 days. At that moment, the activities of lipase in liver and gastrointestinal tract also reached their highest points which were 1.5 and 1.4 times of S0 group(P<0.05), respectively. Then the consumption of crude fat significantly reduced(P<0.05), and lipase activity also plummeted. 3) With the declining of crude protein and crude fat contents, the content of ash increased continuously which strongly increased 1.7 times in hunger 3 days compared S0 group(P<0.05). 4) The nitrogen-free extract content was a non-significant reduced in earlier starvation period(P>0.05), while it decreased to 0.61% in the 9th day, and the relative loss rate of crude protein was up to 28.15%(P<0.05). At that moment, amylase activity in liver increased from 0.31 U/mg(S0 group) to 0.69 U/mg, which reached the peak. 5) Change of energy content in fish body confirmed the energy utilization pattern of starvation juvenile P. argenteus. Thus survival strategy of juvenile P. argenteus in starvation is that it mainly consumes the fat as energy in earlier starvation period, and uses the protein as the main energy with the prolongation of starvation. With the close to the limit in use of protein and fat, it can only increase the carbohydrate utilization in later starvation period.

Key words： Pampus argenteus; starvation stress; biochemical components; digestive enzyme activities; survival strategy

参考文献

[1] 龙章强,彭士明,陈立侨,等.饥饿与再投喂对黑鲷幼鱼体质量变化、生化组成及肝脏消化酶活性的影响[J].中国水产科学,2008,15(4):606-614.
[2] 柳敏海,罗海忠,傅荣兵,等.短期饥饿胁迫对鮸鱼生化组成、脂肪酸和氨基酸组成的影响[J].水生生物学报,2009,33(2):230-235.
[3] EINEN O,WAAGAN B,THOMASSEN M S.Starvation prior to slaughter in Atlantic salmon(Salmo salar) Ⅰ.Effects on weight loss,body shape,slaughter- and fillet-yield,proximate and fatty acid composition[J].Aquaculture,1998,166(1/2):85-104.
[4] 王文,王倩倩,张玉蓉,等.饥饿对尼罗罗非鱼幼鱼粗化学成分的影响[J].重庆师范大学学报:自然科学版,2012,29(5):20-25.
[5] TIAN X L,FANG J H,DONG S L.Effects of starvation and recovery on the growth,metabolism and energy budget of juvenile tongue sole(Cynoglossus semilaevis)[J].Aquaculture,2010,310(1/2):122-129.
[6] 邓利,张波,谢小军.南方鲇继饥饿后的恢复生长[J].水生生物学报,1999,23(2):167-173.
[7] 钱云霞.饥饿对养殖鲈蛋白酶活力的影响[J].水产科学,2002,21(3):6-7.
[8] 高露姣,陈立侨,赵晓勤,等.施氏鲟幼鱼的饥饿和补偿生长研究——对消化器官结构和酶活性的影响[J].中国水产科学,2004,11(5):413-419.
[9] 郑曙明,王燕妮,聂迎霞,等.虎鲨饥饿后的补偿生长及淀粉酶活性研究[J].华中农业大学学报,2003,22(5):483-487.
[10] 柴鹏,李吉方,吴蒙蒙,等.饥饿和再投喂对锦鲤幼鱼几种消化酶活性的影响[J].水利渔业,2007,27(4):12-14.
[11] 区又君,刘泽伟.饥饿和再投喂对千年笛鲷幼鱼消化酶活性的影响[J].海洋学报,2007,29(1):86-91.
[12] 关胜军,吴锐全,谢骏,等.饥饿对大口黑鲈消化器官、蛋白酶和淀粉酶活力的影响[J].南方水产,2007,3(2):25-29.
[13] CARUSO G,DENARO M G,CARUSO R,et al.Changes in digestive enzyme activities of red porgy Pagrus pagrus during a fasting-refeeding experiment[J].Fish Physiology and Biochemistry,2014,40(5):1373-1382.

[14] YENGKOKPAM S,DEBNATH D,PAL A K,et al.Short-term periodic feed deprivation in Labeo rohita fingerlings:effect on the activities of digestive,metabolic and anti-oxidative enzymes[J].Aquaculture,2013,412/413:186-192.
[15] 施兆鸿,赵峰,傅荣兵,等.银鲳人工育苗技术研究[J].海洋渔业,2009,31(1):53-57.
[16] 施兆鸿,彭士明,王建钢,等.人工养殖银鲳子代胚胎发育及仔稚幼鱼形态观察[J].中国水产科学,2011,18(2):267-274.
[17] 李明华.科威特开展银鲳人工养殖技术研究现状[J].中国水产,2006,372(11):54-57.
[18] 祝世军.工厂化室内育苗池养殖银鲳试验[J].河北渔业,2008(3):19-20.
[19] ZHAO F,DONG Y H,ZHUANG P,et al.Genetic diversity of silver pomfret(Pampus argenteus) in the southern Yellow and East China Seas[J].Biochemical Systematics and Ecology,2011,39(2):145-150.

[20] SUN P,YIN F,GAO Q X,et al.Genetic diversity and population structure of silver pomfret(Pampus argenteus) in the Indo-West Pacific revealed by mitochondrial control region sequences[J].Biochemical Systematics and Ecology,2013,51:28-36.
[21] 崔青曼,袁春营,李小双.渤海银鲳生殖力的研究[J].海洋通报,2009,28(6):66-69.
[22] 赵峰,施兆鸿,庄平.银鲳繁育生物学研究进展[J].海洋科学,2010,34(1):90-96.
[23] JAMES C M,ALMATAR S M.Potential of silver pomfret(Pampus argenteus) as a new candidate species for aquaculture[J].Aquaculture Asia,2008,13(2):49-50.
[24] 黄旭雄,施兆鸿,李伟微,等.银鲳亲鱼不同组织的氨基酸及其随性腺发育的变化[J].水产学报,2009,33(2):278-287.
[25] ZHAO F,ZHUANG P,SONG C,et al.Amino acid and fatty acid compositions and nutritional quality of muscle in the pomfret,Pampus punctatissimus[J].Food Chemistry,2010,118(2):224-227.

[26] 徐善良,王丹丽,徐继林,等.东海银鲳(Pampus argenteus)、灰鲳(P.cinereus)和中国鲳(P.sinensis)肌肉主要营养成分分析与评价[J].海洋与湖沼,2012,43(4):775-782.
[27] 钱周兴,徐永清,方一峰.饥饿对鱼类的影响[J].生物学通报,2006,41(6):9-11.
[28] 谭肖英,罗智,王为民,等.饥饿对小规格斑点叉尾体重及鱼体生化组成的影响[J].水生生物学报,2009,33(1):39-45.
[29] 高露姣,陈立侨,宋兵,等.饥饿和补偿生长对史氏鲟幼鱼摄食、生长和体成分的影响[J].水产学报,2004,28(3):279-284.
[30] 楼宝,史会来,毛国民,等.饥饿及恢复投饵过程中花鲈肌肉组成及非特异免疫水平的变化[J].水产学报,2008,32(6):929-938.
[31] 王志铮,施建军,吕敢堂,等.受短期饥饿胁迫下麦瑞加拉鲮鱼(Cirrhina mrigola)幼鱼的生长、肌体组分及其内脏消化酶活力的变化特征[J].海洋与湖沼,2006,37(3):218-224.
[32] MADDOCK D M,BURTON M P M.Some effects of starvation on the lipid and skeletal muscle layers of the winter flounder,Pleuronectes americanus[J].Canadian Journal of Zoology,1994,72(9):1672-1679.

[33] 张守领,顾若波,徐钢春,等.饥饿对于花肌肉和血清主要生化指标的影响[J].上海交通大学学报:农业科学版,2006,24(6):513-516.
[34] 邓利,张为民,林浩然.饥饿对黑鲷血清生长激素、甲状腺激素以及白肌和肝脏脂肪、蛋白质含量的影响[J].动物学研究,2003,24(2):94-98.
[35] 刘波,何庆国,唐永凯,等.饥饿胁迫对吉富罗非鱼生长及生理生化指标的影响[J].中国水产科学,2009,16(2):230-237.
[36] 周爱国,茅沈丽,王超,等.饥饿胁迫对杂交鳢生长及生化组成的影响[J].水生态学杂志,2012,33(5):78-82.
[37] 黄权,高峰,孟繁伊,等.饥饿和再投喂对花羔红点鲑肌肉组分和血液指标的影响[J].吉林农业大学学报,2009,31(4):460-466,475.
[38] 张波,孙耀,唐启升.饥饿对真鲷生长及生化组成的影响[J].水产学报,2000,24(3):206-210.
[39] WEATHERLEY A H,GILL H S.Recovery growth following periods of restricted rations and starvation in rainbow trout Salmo gairdneri Richardson[J].Journal of Fish Biology,1981,18(2):195-208.

[40] 仇明,封功能,张启焕,等.饥饿胁迫对瓦氏黄颡鱼(Pelteobagrus vachelli)幼鱼肌肉营养成分及血液指标的影响[J].饲料工业,2011,32(14):41-43.
[41] 孙向军,梁拥军,张升利,等.饥饿后再投喂对星斑川鲽生化组成以及能值的影响[J].渔业科学进展,2011,32(1):24-31.
[42] 万丽娟.饥饿和恢复投喂对萍乡肉红鲫补偿生长的影响[D].硕士学位论文.南昌:南昌大学,2007.
[43] 王燕妮,张志蓉,郑曙明.鲤鱼的补偿生长及饥饿对淀粉酶的影响[J].水利渔业,2001,21(5):6-7.

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