本试验旨在研究能量限制对三黄鸡补偿生长及肠道结构的影响,选用72只15日龄三黄母鸡,随机分为3个组:自由采食组(对照组)、15%能量限制组(试验1组)和30%能量限制组(试验2组),限饲15 d(30日龄)、补偿生长14 d(44日龄)和35 d(65日龄)后分别屠宰,取其十二指肠、空肠、回肠,测量其肠壁厚度、绒毛高度和隐窝深度。结果表明:1)限饲降低三黄鸡平均日增重(P<0.05),但料重比与对照组相比差异不显著(P>0.05)。补偿生长后,试验组与对照组组间的末重、平均日增重、料重比均差异不显著(P>0.05)。2)限饲增加了十二指肠、空肠、回肠绒毛高度及回肠绒毛高度/隐窝深度(P>0.05)。补偿生长后,十二指肠肠壁厚度变薄(P>0.05),空肠和回肠绒毛高度增加(P>0.05)。本试验表明,能量限制显著降低三黄鸡限饲期生长性能,补偿生长35 d后表现出完全补偿生长效应;能量限制在一定程度上改善了三黄鸡的小肠肠道形态结构,增加了十二指肠、空肠、回肠绒毛高度。
This experiment was conducted to study the effects of feed restriction on compensatory growth and intestinal structure in Sanhuang chicken. The 72 female Sanhuang chickens at 15 days of age were fed in this experiment. The chickens were randomly assigned to 3 groups: ad libitum (control group), 15% feed restriction (trial group 1) and 30% feed restriction (trial group 2), chickens were slaughtered to measure intestine wall thickness, villus height, crypt depth of duodenum, jejunum, ileum in feed restriction for 15 days (30 day-old), compensatory growth for 14 days (44-day-old), compensatory growth for 35 days (65-day-old), respectively. The results were showed as follows: 1) the average daily gain (ADG) of two experiment groups after feed restriction was significantly lower than that of control group (P<0.05), while there was no significant difference in feed to gain ratio (F/G) (P>0.05). After compensatory growth, there were no significant differences among experiment groups and control group of final weight, ADG and F/G (P>0.05). 2) The villus height of duodenum, jejunum, ileum, and villus height/crypt depth of ileum of chickens were increased by feed restriction (P>0.05). And the intestine wall thickness of duodenum was decreased after compensatory growth, the villus height of jejunum, ileum was increased after compensatory growth (P>0.05). This experiment shows that feed restriction can significantly reduce the growth performance of Sanhuang chickens in feed restriction period, and show a total compensatory growth effect after compensatory growth for 35 d; feed restriction improve the intestinal structure of chickens at a certain extent, enhance the villus height of duodenum, jejunum and ileum.
[1] INGRAM D K,CUTLER R G,WEINDRUCH R,et al.Dietary restriction and aging:the initiation of a primate study[J].Journal of Gerontology,1990,45(5):B148-B163.
[2] VELTHUIS-TE WIERIK E J,VAN DEN BERG H,SCHAAFSMA G,et al.Energy restriction,a useful intervention to retard human ageing?Results of a feasibility study[J].EuropeanJournal of Clinical Nutrition,1994,48(2):138-148.
[3] HANSEN B C,ORTMEYER H K,BODKIN N L.Prevention of obesity in middle-aged monkeys:food intake during body weight clamp[J].Obesity Research,1995,3(Suppl.2):199s-204s.
[4] SANTOSO U.Effects of early feed restriction On growth,fat accumulation and meat composition in unsexed broiler chickens[J].Asian-Australasian Journal of Animal Sciences,2001,14(11):1585-1591.
[5] 潘家强.早期限饲、肉鸡肺小动脉重构和肺动脉高压综合征关系的研究[D].博士学位论文.南京:南京农业大学,2005.
[6] URDANETA-RINCON M,LEESON S.Quantitative and qualitative feed restriction on growth characteristics of male broiler chickens[J].Poultry Science,2002,81(5):679-688.
[7] SANTOSO U.Effects of early feed restriction on the occurrence of compensatory growth,feed conversion efficiency,leg abnormality and mortality in unsexed broiler chickens reared in cages[J].Asian-Australasian Journal of Animal Sciences,2002,15(9):1319-1325.
[8] GOVAERTS T,ROOM G,BUYSE J,et al.Early and temporary quantitative food restriction of broiler chickens.2.Effects on allometric growth and growth hormone secretion[J].Britain Poultry Science,2000,41(3):355-362.
[9] JONES G P,FARRELL D J.Early-life food restriction of broiler chickens.Ⅰ.Methods of application,amino acid supplementation and the age at which restrictions should commence [J].Britain Poultry Science,1992,33:579-587.
[10] 李可洲,李宁,黎介寿,等.短链脂肪酸对大鼠移植小肠形态及功能的作用研究[J].世界华人消化杂志,2002,10(6):720-722.
[11] 杨宁.家禽生产学[M].北京:中国农业出版社,2010.
[12] PLAVNIK I,HURWITZ S.Effect of dietary protein,energy,and feed pelleting on the response of chicks to early feed restriction[J].Poultry Science,1989,68(8):1118-1125.
[13] PLAVNIK I,HURWITZ S.Early feed restriction in chicks:effect of age,duration,and sex[J].Poultry Science,1988,67(3):384-390.
[14] PLAVNIK I,MCMURTRY J P,ROSEBROUGH R W.Effects of early feed restriction in broilers.Ⅰ.Growth performance and carcass composition[J].Growth,1986,50(1):68-76.
[15] PLAVNIK I,HURWITZ S.The performance of broiler chicks during and following a severe feed restriction at an early age[J].Poultry Science,1985,64:348-355.
[16] BALOG J M,ANTHONY N B,COOPER M A,et al.Ascites syndrome and related pathologies in feed restricted broilers raised in a hypobaric chamber[J].Poultry Science,2000,79(3):318-323.
[17] 王俊东.兽医药实验室检验技术[M].北京:中国农业科学技术出版社,2005:160-218.
[18] 马仲华,沈和相.家畜解剖学及组织胚胎学[M].2版.北京:中国农业出版社,2000.
[19] 王佳伟,黄艳群,陈文,等.限饲对肉仔鸡生产性能及部分血清生化指标的影响[J].扬州大学学报:农业与生命科学版,2009,30(4):30-34.
[20] 陈继兰,吕连山,赵玲,等.石岐黄肉鸡前期日粮适宜的能量和蛋白质水平的研究[J].中国畜牧杂志,1998,34(4):10-11.
[21] SUSBILLA J P,FRANKEL T L,PARKINSON G,et al.Weight of internal organs and carcase yield of early food restricted broilers broilers[J].Britain Poultry Science,1994,35(5):677-685.
[22] 莫棣华.对"广黄"鸡的能量和蛋白质需要的研究[J].广东农业科学,1983(3):27-30.
[23] DEATON J W.The effect of early feed restriction on broiler performance[J].Poultry Science,1995,74(8):1280-1286.
[24] 苏瑛,罗东君,刘俊琼.肉仔鸡早期限制饲养的研究--限饲对肉仔鸡生产性能、腹脂垫及消化道的影响[J].中国饲料,1996(14):20-23.
[25] LIPPENS M,ROOM G,DE GROOTE G,et al.Early and temporary quantitative food restriction of broiler chickens.1.Effects on performance characteristics,mortality and meat quality[J].Britain Poultry Science,2000,41(3):343-354.
[26] 于昱,吕林,罗绪刚,等.锌在肉仔鸡小肠不同部位吸收机理的研究[J].中国农业科学,2008,41(9):2789-2797.
[27] 杨秀平,肖向红.动物生理学[M].北京:高等教育出版社,2009.2.
[28] 魏刚才,郑素玲.家禽肠道黏膜的作用及保护[J].中国家禽,2007,29(11):47-49.
[29] 黄奕生.畜禽组织学与胚胎学[M].成都:成都科技大学出版社,1990.
[30] 朱宪章,刘路路,王志祥,等.发酵饲料对朗德鹅生长性能、产肝性能、肠道黏膜结构及组织器官ATP酶活性的影响[J].动物营养学报,2013,25(11):2668-2674.
[31] 张书汁.能量限制对肉鸡肠道结构及血液生化指标的影响[D].硕士学位论文.郑州:河南农业大学,2009.
[32] HAMPSON D J.Alterations in piglet small intestinal structure at weaning[J].Research in Veterinary Science,1986,40(1):32-40.
[33] 王子旭.锌硒互作对肉鸡肠黏膜结构及黏膜免疫相关细胞影响的研究[D].硕士学位论文.北京:中国农业大学,2003.
[34] 马治宇.乳酸菌及其培养液对肉鸡生产性能、肠道菌群及肠道结构的影响[D].硕士学位论文.杨凌:西北农林科技大学,2008.
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