综述 Review

牛链球菌在瘤胃中产酸的代谢机制及调控

展开
  • 扬州大学动物科学与技术学院, 扬州 225009
陈连民(1991-),男,江苏阜宁人,硕士研究生,研究方向为反刍动物瘤胃微生物代谢调控。E-mail:LianminChen@yeah.net

收稿日期: 2015-09-29

  网络出版日期: 2016-03-14

基金资助

江苏省研究生实践创新计划(SJLX15_0674);国家自然科学基金(31572429);公益性行业(农业)科研专项(201303144);江苏省优势学科建设项目(PADA)

Metabolic Mechanism of Acid Production by Streptococcus bovis in Rumen and Its Regulation

Expand
  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2015-09-29

  Online published: 2016-03-14

摘要

牛链球菌(S. bovis)是瘤胃主要的乳酸产生菌,在饲喂高精料饲粮导致瘤胃乳酸中毒进程中扮演重要角色。已有研究证实S. bovis利用碳水化合物代谢产酸主要受葡萄糖转运方式、酵解产酸途径中酶和中间代谢物调控。另外,研究也发现环境pH、增殖生长阶段及分解代谢控制蛋白(CcpA)等对其产酸速率和模式也有显著影响。本文对近年来有关S. bovis利用饲料中碳水化合物发酵产酸代谢途径及影响因素研究加以综述,为从微生物代谢角度解析瘤胃乳酸中毒机制提供参考。

本文引用格式

陈连民, 沈宜钊, 王洪荣 . 牛链球菌在瘤胃中产酸的代谢机制及调控[J]. 动物营养学报, 2016 , 28(3) : 665 -673 . DOI: 10.3969/j.issn.1006-267x.2016.03.005

Abstract

Streptococcus bovis (S. bovis) is usually a major lactate producing bacterium in the rumen, and is recognized its' contribution to development of rumen acidosis when ruminants are fed high concentrate diets. Previous work indicates that carbohydrate metabolism in S. bovis is mainly affected by the way of glucose trans membrane transport, and enzymes and intermediate metabolites in glycolytic pathway. In addition, the factors, environmental pH, growth stage, and control protein catabolism (CcpA), etc., also have significant impacts. In this paper, metabolic mechanism and influence factors of carbohydrate fermentation and acid production by S. bovis were reviewed in purpose to provide references for further insight into the mechanism of rumen acidosis caused by lactic acids.

参考文献

[1] MAROUNE M,BARTOS S.Interactions between rumen amylolytic and lactate-utilizing bacteria in growth on starch[J].Journal of Applied Bacteriology,1987,63(3):233-238.  

[2] WANG H R,PAN X H,WANG C,et al.Effects of different dietary concentrate to forage ratio and thiamine supplementation on the rumen fermentation and ruminal bacterial community in dairy cows[J].Animal Production Science,2014,55(2):189-193.

[3] 王洪荣.反刍动物瘤胃酸中毒机制解析及其营养调控措施[J].动物营养学报,2014,26(10):3140-3148.

[4] LETTAT A,NOZIÈRE P,SILBERBERG M,et al.Rumen microbial and fermentation characteristics are affected differently by bacterial probiotic supplementation during induced lactic and subacute acidosis in sheep[J].BMC Microbiology,2012,12:142.

[5] ASANUMA N,HINO T.Understanding metabolic regulation in the ruminal bacteria,Streptococcus bovis,Selenomonas ruminantium,and Megasphaera elsdenii[M]//MARTIN S A.Gastrointestinal microbiology in animals.Kerala,India:Research Signpost,2002:61-87.

[6] RUSSELL J B.Low-affinity,high-capacity system of glucose transport in the ruminal bacterium Streptococcus bovis:evidence for a mechanism of facilitated diffusion[J].Applied and Environmental Microbiology,1990,56(11):3304-3307.

[7] VADEBONCOEUR C,PELLETIER M.The phosphoenolpyruvate:sugar phosphotransferase system of oral Streptococci and its role in the control of sugar metabolism[J].FEMS Microbiology Reviews,1997,19(3):187-207.

[8] POSTMA P W,LENGELER J W,JACOBSON G R.Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria[J].Microbiological Reviews,1993,57(3):543-594.

[9] DEUTSCHER J,SAIER M H,Jr.ATP-dependent protein kinase-catalyzed phosphorylation of a seryl residue in HPr,a phosphate carrier protein of the phosphotransferase system in Streptococcus pyogenes[J].Proceedings of the National Academy of Sciences of the United States of America,1983,80(22):6790-6794.  

[10] FUJITA Y,MIWA Y,GALINIER A,et al.Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr[J].Molecular Microbiology,1995,17(5):953-960.  

[11] MARTIN-VERSTRAETE I,STVLKE J,KLIER A,et al.Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon[J].Journal of Bacteriology,1995,177(23):6919-6927.

[12] DEUTSCHER J,KVSTER E,BERGSTEDT U,et al.Protein kinase-dependent HPr/CcpA interaction links glycolytic activity to carbon catabolite repression in gram-positive bacteria[J].Molecular Microbiology,1995,15(6):1049-1053.  

[13] HUECK C J,HILLEN W,SAIER M H,Jr.Analysis of a cis-active sequence mediating catabolite repression in gram-positive bacteria[J].Research in Microbiology,1994,145(7):503-518.  

[14] YE J J,REIZER J,CUI X,et al.Inhibition of the phosphoenolpyruvate:lactose phosphotransferase system and activation of a cytoplasmic sugar-phosphate phosphatase in Lactococcus lactis by ATP-dependent metabolite-activated phosphorylation of serine 46 in the phosphocarrier protein HPr[J].Journal of Biological Chemistry,1994,269(16):11837-11844.

[15] YE J J,SAIER M H,Jr.Purification and characterization of a small membrane-associated sugar phosphate phosphatase that is allosterically activated by HPr (Ser (P)) of the phosphotransferase system in Lactococcus lactis[J].Journal of Biological Chemistry,1995,270(28):16740-16744.  

[16] COOK G M,KEARNS D B,RUSSELL J B,et al.Regulation of the lactose phosphotransferase system of Streptococcus bovis by glucose:independence of inducer exclusion and expulsion mechanisms[J].Microbiology,1995,141(9):2261-2269.  

[17] ASANUMA N,HINO T.Molecular characterization of HPr and related enzymes,and regulation of HPr phosphorylation in the ruminal bacterium Streptococcus bovis[J].Archives of Microbiology,2003,179(3):205-213.

[18] 王镜岩,朱圣庚,徐长法.生物化学:下册[M].3版.北京:高等教育出版社,2002.

[19] ZHOU S D,CAUSEY T B,HASONA A,et al.Production of optically pure D-lactic acid in mineral salts medium by metabolically engineered Escherichia coli W3110[J].Applied and Environmental Microbiology,2003,69(1):399-407.  

[20] ZHOU S D,SHANMUGAM K T,INGRAM L O.Functional replacement of the Escherichia coliD-(-)-lactate dehydrogenase gene (ldhA) with the L-(+)-lactate dehydrogenase gene (ldhL) from Pediococcus acidilactici[J].Applied and Environmental Microbiology,2003,69(4):2237-2244.

[21] OKANO K,TANAKA T,OGINO C,et al.Biotechnological production of enantiomeric pure lactic acid from renewable resources:recent achievements,perspectives,and limits[J].Applied Microbiology and Biotechnology,2010,85(3):413-423.  

[22] AXELSSON L.Lactic acid bacteria:classification and physiology[M].New York:Marcel Dekker,2004:1-66.

[23] LEVERING J,MUSTERS M W J M,BEKKER M,et al.Role of phosphate in the central metabolism of two lactic acid bacteria-a comparative systems biology approach[J].FEBS Journal,2012,279(7):1274-1290.  

[24] 包尔德文.动态生物化学[M].石声汉,译.北京:人民卫生出版社,1956.

[25] 李建武.生物化学[M].北京:北京大学出版社,1990.

[26] HORTON R H,MORAN L A,OCHS R S,et al.Principles of biochemistry[M].3rd ed.Upper Saddle River:Prentice Hall,1996.

[27] NELSON D L,LEHNINGER A L,COX M M.Lehninger principles of biochemistry[M].New York:W.H.Freeman and Company,2008.

[28] RUSSELL J B,HINO T.Regulation of lactate production in Streptococcus bovis:a spiraling effect that contributes to rumen acidosis[J].Journal of Dairy Science,1985,68(7):1712-1721.  

[29] WOLIN M J.Fructose-1,6-diphosphate requirement of streptococcal lactic dehydrogenases[J].Science,1964,146(3645):775-777.  

[30] ASANUMA N,HINO T.Effects of pH and energy supply on activity and amount of pyruvate formate-lyase in Streptococcus bovis[J].Applied and Environmental Microbiology,2000,66(9):3773-3777.  

[31] FORDYCE A M,CROW V L,THOMAS T D.Regulation of product formation during glucose or lactose limitation in nongrowing cells of Streptococcus lactis[J].Applied and Environmental Microbiology,1984,48(2):332-337.

[32] TAKAHASHI S,ABBE K,YAMADA T.Purification of pyruvate formate-lyase from Streptococcus mutans and its regulatory properties[J].Journal of Bacteriology,1982,149(3):1034-1040.

[33] THOMAS T D,TURNER K W,CROW V L.Galactose fermentation by Streptococcus lactis and Streptococcus cremoris:pathways,products,and regulation[J].Journal of Bacteriology,1980,144(2):672-682.

[34] RAMOS A,NEVES A R,VENTURA R,et al.Effect of pyruvate kinase overproduction on glucose metabolism of Lactococcus lactis[J].Microbiology,2004,150(4):1103-1111.  

[35] ASANUMA N,KANADA K,HINO T.Molecular properties and transcriptional control of the phosphofructokinase and pyruvate kinase genes in a ruminal bacterium,Streptococcus bovis[J].Anaerobe,2008,14(4):237-241.  

[36] ASANUMA N,HINO T.Fructose bisphosphate aldolase activity and glycolytic intermediate concentrations in relation to lactate production in Streptococcus bovis[J].Anaerobe,2002,8(1):1-8.  

[37] ASANUMA N,YOSHII T,KIKUCHI M,et al.Effects of the overexpression of fructose-1,6-bisphosphate aldolase on fermentation pattern and transcription of the genes encoding lactate dehydrogenase and pyruvate formate-lyase in a ruminal bacterium,Streptococcus bovis[J].The Journal of General and Applied Microbiology,2004,50(2):71-78.  

[38] ASANUMA N,YOSHII T,HINO T.Molecular characteristics and transcription of the gene encoding a multifunctional alcohol dehydrogenase in relation to the deactivation of pyruvate formate-lyase in the ruminal bacterium Streptococcus bovis[J].Archives of Microbiology,2004,181(2):122-128.  

[39] ASANUMA N,HINO T.Molecular characterization and expression of pyruvate formate-lyase-activating enzyme in a ruminal bacterium,Streptococcus bovis[J].Applied and Environmental Microbiology,2002,68(7):3352-3357.  

[40] ASANUMA N,YOSHIZAWA K,HINO T.Properties and role of glyceraldehyde-3-phosphate dehydrogenase in the control of fermentation pattern and growth in a ruminal bacterium,Streptococcus bovis[J].Current Microbiology,2009,58(4):283-287.  

[41] GARRIGUES C,LOUBIERE P,LINDLEY N D,et al.Control of the shift from homolactic acid to mixed-acid fermentation in Lactococcus lactis:predominant role of the NADH/NAD+ ratio[J].Journal of Bacteriology,1997,179(17):5282-5287.

[42] KANDLER O.Carbohydrate metabolism in lactic acid bacteria[J].Antonie van Leeuwenhoek,1983,49(3):209-224.  

[43] ASANUMA N,IWAMOTO M,HINO T.Regulation of lactate dehydrogenase synthesis in a ruminal bacterium,Streptococcus bovis[J].The Journal of General and Applied Microbiology,1997,43(6):325-331.  

[44] RUSSELL J B,COTTA M A,DOMBROWSKI D B.Rumen bacterial competition in continuous culture:Streptococcus bovis versus Megasphaera elsdenii[J].Applied and Environmental Microbiology,1981,41(6):1394-1399.

[45] WELLS J E,KRAUSE D O,CALLAWAY T R,et al.A bacteriocin-mediated antagonism by ruminal lactobacilli against Streptococcus bovis[J].FEMS Microbiology Ecology,1997,22(3):237-243.  

[46] MAO S Y,ZHANG R Y,WANG D S,et al.Impact of subacute ruminal acidosis (SARA) adaptation on rumen microbiota in dairy cattle using pyrosequencing[J].Anaerobe,2013,24:12-19.

[47] SUN Y Z,MAO S Y,ZHU W Y.Rumen chemical and bacterial changes during stepwise adaptation to a high-concentrate diet in goats[J].Animal,2010,4(2):210-217.  

[48] LIU J H,XU T T,ZHU W Y,et al.A high-grain diet alters the omasal epithelial structure and expression of tight junction proteins in a goat model[J].The Veterinary Journal,2014,201(1):95-100.  

[49] ASANUMA N,IWAMOTO M,HINO T.Structure and transcriptional regulation of the gene encoding pyruvate formate-lyase of a ruminal bacterium,Streptococcus bovis[J].Microbiology,1999,145(1):151-157.  

[50] ASANUMA N,YOSHII T,HINO T.Molecular characterization and transcription of the luxS gene that encodes LuxS autoinducer 2 synthase in Streptococcus bovis[J].Current Microbiology,2004,49(5):366-371.

[51] HENKIN T M,GRUNDY F J,NICHOLSON W L,et al.Catabolite repression of α amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacl and galR repressors[J].Molecular Microbiology,1991,5(3):575-584.  

[52] ASANUMA N,YOSHII T,HINO T.Molecular characterization of CcpA and involvement of this protein in transcriptional regulation of lactate dehydrogenase and pyruvate formate-lyase in the ruminal bacterium Streptococcus bovis[J].Applied and Environmental Microbiology,2004,70(9):5244-5251.  

[53] ASANUMA N,HINO T.Presence of NADP+-specific glyceraldehyde-3-phosphate dehydrogenase and CcpA-dependent transcription of its gene in the ruminal bacterium Streptococcus bovis[J].FEMS Microbiology Letters,2006,257(1):17-23.  

[54] ASANUMA N,KANADA K,ARAI Y,et al.Molecular characterization and significance of phosphoenolpyruvate carboxykinase in a ruminal bacterium,Streptococcus bovis[J].The Journal of General and Applied Microbiology,2010,56(2):121-127.
文章导航

/