姜黄素对早期断奶仔猪回肠黏膜形态、紧密连接蛋白和炎性因子基因表达以及血清免疫球蛋白水平的影响

荀文娟, 周汉林, 侯冠彧, 曹婷, 施力光, 赵春萍

doi:10.3969/j.issn.1006-267x.2016.03.023

动物营养学报 >

2016 , Vol. 28 >Issue 3: 826 - 833

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.03.023

研究简报 Short Communications

姜黄素对早期断奶仔猪回肠黏膜形态、紧密连接蛋白和炎性因子基因表达以及血清免疫球蛋白水平的影响

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1. 中国热带农业科学院热带作物品种资源研究所, 儋州 571737;
2. 海南大学农学院, 海口 570228

荀文娟(1985-),女,山西交城人,副研究员,博士,研究方向为动物繁殖营养调控。E-mail:xunwenjuan991@163.com

收稿日期: 2015-09-25

网络出版日期: 2016-03-14

基金资助

国家自然科学基金(31301990);海南省自然科学基金项目(20153079);中国热带农业科学院中央级公益性科研院所基本科研业务费专项(1630032013001,1630032013020)

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Effects of Curcumin on Ileum Mucosal Morphology, Gene Expression of Tight Junction Proteins and Inflammatory Cytokines, and Serum Immunoglobulin Levels of Early Weaning Piglets

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1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China;
2. College of Agriculture, Hainan University, Haikou 570228, China

Received date: 2015-09-25

Online published: 2016-03-14

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摘要

本试验旨在研究姜黄素对早期断奶仔猪回肠黏膜形态、紧密连接蛋白和炎性因子基因表达以及血清免疫球蛋白水平的影响。选取50头胎次、体重相近的21日龄"杜×长×大"健康断奶仔猪,公母各占1/2,随机分为5组,每组10个重复,每个重复1头猪。对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加50 mg/kg喹烯酮及200、300和400 mg/kg姜黄素的饲粮。预试期7 d,于第4天接种大肠杆菌;正试期21 d。结果表明:1)与对照组相比,300和400 mg/kg姜黄素组回肠绒毛高度和绒毛高度/隐窝深度显著提高(P<0.05),绒毛宽度和隐窝深度显著降低(P<0.05),回肠黏膜紧密连接闭锁蛋白(occludin)、闭锁小带蛋白(ZO-1)mRNA相对表达量显著提高(P<0.05),白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和Toll样受体4(TLR4)mRNA相对表达量显著降低(P<0.05),白细胞介素-10(IL-10)mRNA相对表达量、血清免疫球蛋白G(IgG)和免疫球蛋白M(IgM)水平显著提高(P<0.05);2)喹烯酮组回肠绒毛宽度和TLR4 mRNA相对表达量显著低于对照组(P<0.05),其余各项指标与对照组相比差异不显著(P>0.05)。结果显示:添加300或400 mg/kg姜黄素可改善回肠黏膜上皮形态,增加肠黏膜屏障完整性,提高仔猪免疫力,其作用效果优于喹烯酮。

关键词： 姜黄素; 断奶仔猪; 回肠黏膜形态; 紧密连接蛋白; 炎性因子; 免疫球蛋白

本文引用格式

荀文娟, 周汉林, 侯冠彧, 曹婷, 施力光, 赵春萍 . 姜黄素对早期断奶仔猪回肠黏膜形态、紧密连接蛋白和炎性因子基因表达以及血清免疫球蛋白水平的影响[J]. 动物营养学报, 2016 , 28(3) : 826 -833 . DOI: 10.3969/j.issn.1006-267x.2016.03.023

Abstract

This experiment was conducted to study the effects of curcumin on ileum mucosal morphology, gene expression of tight junction proteins and inflammatory cytokines, and serum immunoglobulin levels of early weaning piglets. Fifty Duroc×Landrace×Large White healthy piglets (male to female ratio was 1 : 1) with similar parity and body weight, weaned at 21 days of age, were randomly allocated into 5 groups with 10 replicates in each group and 1 pig in each replicate. The dietary treatments were the basal diet (control group), and the basal diet supplemented with 50 mg/kg quinocetone, or 200, 300 or 400 mg/kg curcumin (experimental groups). The preliminary trial lasted for 7 days and piglets were orally challenged with enterotoxigenic Escherichia coli on the fourth day. The experiment lasted for 21 days. The results showed as follows: 1) compared with the control group, supplementation with 300 or 400 mg/kg curcumin significantly improved villus height and villus height/crypt depth ratio (V/C) (P<0.05), significantly reduced crypt depth and villus width(P<0.05), significantly increased the relative expression levels of occludin and zonula occluden-1(ZO-1) mRNA (P<0.05), significantly decreased the relative expression levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and toll-like receptor 4 (TLR4) mRNA (P<0.05), and significantly increased interleukin-10 (IL-10) mRNA relative expression level, serum immunoglobulin G (IgG) and immunoglobulin M (IgM) levels (P<0.05). 2) The villus width and TLR4 mRNA relative expression level were significantly decreased by quinocetone supplementation (P<0.05), but the other indicators were not significantly affected compared with the control group (P>0.05). It is suggested that 300 or 400 mg/kg curcumin supplementation was more effective than quinocetone in improving ileum mucosal barrier integrity, morphology, and immune status of weaning piglets.

Key words： curcumin; weaning piglets; ileal mucosal morphology; tight junction protein; inflammatory cytokine; immunoglobulin

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