动物营养学报 >

2016 , Vol. 28 >Issue 3: 891 - 898

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.03.031

研究简报 Short Communications

亚麻籽油对脂多糖刺激仔猪肝脏Toll样受体4和核苷酸结合寡聚化结构域信号通路关键基因表达的影响

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  • 武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023
陈少魁(1989-),男,湖北荆州人,硕士研究生,从事猪营养生理机能调控的研究。E-mail:loveskchen@163.com

收稿日期: 2015-08-18

  网络出版日期: 2016-03-14

基金资助

湖北省自然科学基金杰出青年人才项目(2013CFA029)

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Effects of Flaxseed Oil on mRNA Expression of Key Genes in TLR4 and NOD Signaling Pathways in Liver of Piglets after LPS Challenge

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  • Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2015-08-18

  Online published: 2016-03-14

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摘要

本试验旨在研究亚麻籽油对脂多糖(LPS)刺激仔猪肝脏Toll样受体4(TLR4)和核苷酸结合寡聚化结构域(NOD)信号通路关键基因表达的影响。选取24头断奶仔猪,按体重相近原则随机分为4个组,分别为对照组、LPS组、2.5%亚麻籽油组(2.5%亚麻籽油+LPS)、5.0%亚麻籽油组(5.0%亚麻籽油+LPS),每组6个重复,每个重复1头猪,试验期21 d。试验组注射100 μg/kg体重的LPS,对照组注射等量的生理盐水。注射LPS或生理盐水4 h后屠宰仔猪,取肝脏,测定TLR4和NOD信号通路关键基因及相关炎性介质的mRNA表达水平。结果表明:1)LPS刺激显著提高了肝脏肿瘤坏死因子-α(TNF-α)、环氧酶2(COX2)、热休克蛋白70(HSP70)的mRNA相对表达量(P<0.05),2.5%亚麻籽油可显著降低COX2、TNF-α的mRNA相对表达量(P<0.05),5.0%亚麻籽油可显著降低TNF-α的mRNA相对表达量(P<0.05)。2)LPS刺激显著提高了肝脏TLR4、髓样分化因子88(MyD88)、白细胞介素-1受体相关激酶1(IRAK1)、NOD1、NOD2、受体互作蛋白2(RIPK2)、核因子-κB(NF-κB)的mRNA相对表达量(P<0.05);2.5%亚麻籽油可显著降低NOD1、NOD2的mRNA相对表达量(P<0.05),有降低RIPK2 mRNA相对表达量的趋势(0.05≤P<0.10);5.0%亚麻籽油可显著降低NOD2的mRNA相对表达量(P<0.05)。这表明LPS刺激导致仔猪发生炎症反应,亚麻籽油可能通过抑制NOD信号通路进而缓解肝脏炎症反应。

关键词： 仔猪; 亚麻籽油; 脂多糖; 肝脏; TLR4; NOD

本文引用格式

陈少魁, 刘玉兰, 王海波, 王秀英, 朱惠玲, 张晶, 王树辉, 涂治骁 . 亚麻籽油对脂多糖刺激仔猪肝脏Toll样受体4和核苷酸结合寡聚化结构域信号通路关键基因表达的影响[J]. 动物营养学报, 2016 , 28(3) : 891 -898 . DOI: 10.3969/j.issn.1006-267x.2016.03.031

Abstract

This experiment was conducted to investigate the effects of flaxseed oil on mRNA expression of key genes in Toll-like receptor 4 (TLR4) and nucleotide binding oligomerization domain protein (NOD) signaling pathways in liver of piglets after lipopolysaccharide (LPS) challenge. Twenty four pigs with similar body weight were randomly divided into 4 groups with 6 replicates per group and 1 pig per replicate, and the 4 groups were control group, LPS group, 2.5% flaxseed oil group (2.5% flaxseed oil + LPS), and 5.0% flaxseed oil group (5.0% flaxseed oil + LPS), respectively. The experiment lasted for 21 days. The pigs in the experimental groups were injected intraperitoneally with 100 μg/kg body weight LPS, whereas the pigs in the control group were injected with an equivalent amount of sterile saline. At 4 h post-challenge, the pigs were slaughtered and liver samples were collected. The mRNA expression levels of inflammatory cytokines and the key genes in TLR4 and NOD signaling pathways were detected by real-time PCR. The results showed as follows: 1) after LPS challenge, the mRNA relative expression levels of cyclo-oxygenase 2 (COX2), heat shock protein 70 (HSP70) and tumor necrosis factor-α (TNF-α) in liver were significantly increased (P<0.05). The mRNA relative expression levels of COX2 and TNF-α in 2.5% flaxseed oil group were significantly lower than those in LPS group (P<0.05), and the TNF-α mRNA relative expression level in 5.0% flaxseed oil group was significantly lower than that in LPS group (P<0.05). 2) After LPS challenge, the mRNA relative expression levels of TLR4, myeloid differentiation factor 88 (MyD88), interleukin-1 receptor-associated kinase 1 (IRAK1), NOD1, NOD2, receptor-interacting serine/threonine-protein kinase 2 (RIPK2) and nuclear factor-κB (NF-κB) in liver were significantly increased (P<0.05). The mRNA relative expression levels of NOD1 and NOD2 in 2.5% flaxseed oil group were significantly lower than those in LPS group (P<0.05), and the mRNA relative expression level of RIPK2 had a tendency to decrease control to the LPS group (0.05≤P<0.10).The mRNA relative expression level of NOD2 in 5.0%flaxseed oil group was significantly lower than that in LPS group (P<0.05). These results indicate that LPS challenge induces inflammatory response in piglets, flaxseed oil can alleviate liver inflammatory response via NOD signaling pathway inhibition.

Key words： piglets; flaxseed oil; LPS; liver; TLR4; NOD

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