黄颡鱼促甲状腺激素受体基因的克隆及其对饲料中添加碘化钾的表达反应

王春玲, 章宏塔, 高有领, 钱国英

doi:10.3969/j.issn.1006-267x.2016.03.037

动物营养学报 >

2016 , Vol. 28 >Issue 3: 940 - 952

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.03.037

研究简报 Short Communications

黄颡鱼促甲状腺激素受体基因的克隆及其对饲料中添加碘化钾的表达反应

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1. 浙江万里学院生物与环境学院, 宁波 315100;
2. 上海海洋大学水产与生命学院, 上海 201306

王春玲(1989-),女,河南周口人,硕士研究生,从事水产动物营养与饲料科学研究,E-mail:chunling209@163.com

收稿日期: 2015-09-15

网络出版日期: 2016-03-14

基金资助

浙江省自然科学基金(LY14C190009);浙江省重大科技专项(2012C12907);宁波市科技局项目(2014A610193,2012B82016)

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Thyrotropin Receptor Gene Cloning and Expression Response to Dietary Potassium Iodide Addition of Yellow Catfish (Pelteobagrus fulvidraco)

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1. College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, China;
2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China

Received date: 2015-09-15

Online published: 2016-03-14

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摘要

本试验旨在得到黄颡鱼促甲状腺激素受体(TSHR)基因的cDNA全长序列,同时掌握其组织表达差异,并揭示饲料中添加碘化钾对黄颡鱼甲状腺TSHR基因表达及生长性能的影响。采用cDNA末端快速扩增(RACE)技术克隆TSHR基因cDNA全长序列,再应用荧光定量PCR技术检测TSHR基因的相对表达量,并制备了碘化钾添加量分别为0(对照)、10、50和100 mg/kg的4种试验饲料进行为期27 d的黄颡鱼养殖试验。结果显示:本试验成功克隆得到长度为2 786 bp的TSHR基因cDNA的全长序列,其中开放阅读框2 238 bp,编码745个氨基酸,Blast程序分析表明黄颡鱼TSHR氨基酸序列与其他已知鱼类的相似性为60%~87%。组织表达分析结果表明TSHR基因在甲状腺组织中的相对表达量较高,其次是肝脏、肌肉和肠道。养殖试验结果表明,100 mg/kg组甲状腺TSHR基因相对表达量显著高于其他各组(P<0.05),50和100 mg/kg组的增重、特定生长率和饲料系数显著优于对照组(P<0.05)。由此得出,饲料中添加适量的碘化钾不仅影响TSHR基因的表达,还能有效促进黄颡鱼的生长。

关键词： 黄颡鱼; 碘化钾; 促甲状腺激素受体; 基因克隆; 基因表达

本文引用格式

王春玲, 章宏塔, 高有领, 钱国英 . 黄颡鱼促甲状腺激素受体基因的克隆及其对饲料中添加碘化钾的表达反应[J]. 动物营养学报, 2016 , 28(3) : 940 -952 . DOI: 10.3969/j.issn.1006-267x.2016.03.037

Abstract

The aims of the present study were to characterize the cDNA full-long sequence of thyrotropin receptor (TSHR) gene of yellow catfish (Pelteobagrus fulvidraco), and to determine the differential expression of TSHR gene in different tissues. In addition, the effects of dietary potassium iodide (KI) addition on TSHR gene expression and growth performance of yellow catfish were revealed. The rapid amplification of cDNA ends (RACE) technique was used to clone the cDNA full-long sequence of TSHR gene. Moreover, RT-qPCR technique was used to determine the relative expression level of TSHR gene. The KI adding levels were set to be 0 (control), 10, 50 and 100 mg/kg in four experimental diets, which were fed to yellow catfish for 27 days. The results showed as follows: the cDNA full-length sequence of TSHR gene was obtained, and it was 2 786 bp, including a complete open reading frame of 2 238 bp encoded a 745 amino acid residues. Blast online analysis indicated that the amino acid sequence of yellow catfish displayed 60% to 87% identity as compared with other known fish species. The analysis results of tissue differential expression of TSHR gene indicated that the highest relative expression level was in thyroid, followed by liver, muscle and intestine. The results of feeding trial were showed that the relative expression level of TSHR gene in thyroid in 100 mg/kg group was significantly higher than that in other groups (P<0.05), and the weight gain, specific growth rate and feed conversion ratio in 50 and 100 mg/kg groups were significantly higher than those in control group (P<0.05). The results in this study imply that the dietary KI in the appropriate amount not only affects the expression of TSHR gene, but also promote the growth of yellow catfish effectively.

Key words： yellow catfish (Pelteobagrus fulvidraco); potasium iodide; thyrotropin receptor; gene clone; gene expression

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