综述 Review

氨基酸影响奶牛乳腺内乳脂合成的机理

  • 赵艳丽 ,
  • 陈璐 ,
  • 史彬林 ,
  • 闫素梅
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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018

收稿日期: 2016-01-22

  网络出版日期: 2016-05-24

基金资助

国家奶业"973计划"项目(2011CB1008003)

Mechanism of Amino Acids Affect Milk Fat Synthesis in Mammary Gland of Dairy Cows

  • ZHAO Yanli ,
  • CHEN Lu ,
  • SHI Binlin ,
  • YAN Sumei
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  • Collage of Animal Science, Inner Mongolia Agriculture University, Hohhot 010018, China

Received date: 2016-01-22

  Online published: 2016-05-24

摘要

乳蛋白前体物主要有游离氨基酸和小肽等。氨基酸不仅能影响乳腺内乳蛋白的合成,而且对乳脂的合成起一定的调控作用。本文主要阐述了氨基酸在乳脂合成过程中的调节作用,并从乳腺对乳脂前体物的摄取规律、乳脂合成相关基因表达、哺乳动物雷帕霉素靶蛋白和腺苷酸活化蛋白激酶信号通路的角度综述了氨基酸对乳脂合成的可能机理,为进一步研究乳脂合成机理及改进牛奶营养品质提供理论依据。

关键词: 奶牛; 氨基酸; 乳脂; 乳腺; 机理

本文引用格式

赵艳丽 , 陈璐 , 史彬林 , 闫素梅 . 氨基酸影响奶牛乳腺内乳脂合成的机理[J]. 动物营养学报, 2016 , 28(5) : 1317 -1323 . DOI: 10.3969/j.issn.1006-267x.2016.05.005

Abstract

Amino acids (AA) and small peptides are the milk protein precursors (MPP) regulating the synthesis of milk fat and milk protein in mammary gland of dairy cows. This paper mainly reviewed the regulation of AA in milk fat synthesis, as well as the mechanism from uptake of the milk fat precursors by the mammary gland, the expression of milk fat relating genes, and the regulation of mammalian target of rapamycin and adenosine monophosphate activated protein kinase signaling pathways, which may provide theory evidence for the mechanism of milk fat synthesis and improve the nutritional quality of milk.

参考文献

[1] HARVATINE K J,ALLEN M S.The effect of production level on feed intake,milk yield,and endocrine responses to two fatty acid supplements in lactating cows[J].Journal of Dairy Science,2005,88(11):4018-4027.  
[2] PURDIE N G,TROUT D R,CIESLAR S R,et al.The effect of short-term hyperammonaemia on milk synthesis in dairy cows[J].Journal of Dairy Research,2009,76(1):49-58.  
[3] WANG M Z,XU B L,WANG H R,et al.Effects of arginine concentration on the in vitro expression of casein and mTOR pathway related genes in mammary epithelial cell from dairy cattle[J].PLoS One,2014,9(5):e95985.
[4] BAUMAN D E,MATHER I H,WALL R J,et al.Major advances associated with the biosynthesis of milk[J].Journal of Dairy Science,2006,89(4):1235-1243.  
[5] PURDIE N G,TROUT D R,POPPI D P,et al.Milk synthetic response of the bovine mammary gland to an increase in the local concentration of amino acids and acetate[J].Journal of Dairy Science,2008,91(1):218-228.  
[6] BIONAZ M,LOOR J J.Gene networks driving bovine mammary protein synthesis during the lactation cycle[J].Bioinformatics and Biology Insights,2011,5:83-98.
[7] BURGOS S A,DAI M,CANT J P.Nutrient availability and lactogenic hormones regulate mammary protein synthesis through the mammalian target of rapamycin signaling pathway[J].Journal of Dairy Science,2010,93(1):153-161.  
[8] 王立娜.氨基酸与STAT5A基因互作对奶牛乳腺上皮细胞泌乳的调节作用及机理[D].博士学位论文.哈尔滨:东北农业大学,2014.
[9] RYZHENKOV V E,SHANYGINA K I,CHISTIAKOVA A M,et al.Effect of arginine on the lipid and lipoprotein content of animal blood[J].Voprosy MeditsinskoǐKhimii,1984,30(6):76-79.
[10] ARAKAWA M,MASAKI T,NISHIMURA J,et al.The effects of branched-chain amino acid granules on the accumulation of tissue triglycerides and uncoupling proteins in diet-induced obese mice[J].Endocrine Journal,2011,58(3):161-170.  
[11] WITTWER F G,GALLARDO P,REYES J,et al.Bulk milk urea concentrations and their relationship with cow fertility in grazing dairy herds in southern Chile[J].Preventive Veterinary Medicine,1999,38(2/3):159-166.
[12] CHAMBERLAIN D G,THOMAS P C.Effect of intravenous supplements of L-methionine on milk yield and composition in cows given silage-cereal diets[J].Journal of Dairy Research,1982,49(1):25-28.  
[13] THOMAS P C,MARTIN P A.The influence of nutrient balance on milk yield and composition[M]//Nutrition and lactation in the dairy cow.Amsterdam:Elsevier,1988:97-118.
[14] 韩慧娜.秸秆日粮条件下阴外动脉灌注乳脂和乳蛋白前体物对奶牛乳腺内短链脂肪酸摄取规律的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2015.
[15] 段斌.阴外动脉氨基酸平衡对奶山羊乳腺摄取乳成分前体物的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2010.
[16] VANHATALO A,VARVIKKO T,HUHTANEN P.Effects of casein and glucose on responses of cows fed diets based on restrictively fermented grass silage[J].Journal of Dairy Science,2003,86(10):3260-3270.  
[17] LEMOSQUET S,RAGGIO G,LOBLEY G E,et al.Whole-body glucose metabolism and mammary Energetic nutrient metabolism in lactating dairy cows receiving digestive infusions of casein and propionic acid[J].Journal of Dairy Science,2009,92(12):6068-6082.  
[18] DOEPEL L,LAPIERRE H.Changes in production and mammary metabolism of dairy cows in response to essential and nonessential amino acid infusions[J].Journal of Dairy Science,2010,93(7):3264-3274.  
[19] GRUMMER R R.Effect of feed on the composition of milk fat[J].Journal of Dairy Science,1991,74(9):3244-3257.  
[20] 孙满吉,卢德勋,王丽芳,等.基础日粮下关中奶山羊乳腺对营养物质摄取和利用的研究[J].动物营养学报,2009,21(6):859-864.
[21] WEEKES T L,LUIMES P H,CANT J P.Responses to amino acid imbalances and deficiencies in lactating dairy cows[J].Journal of Dairy Science,2006,89(6):2177-2187.  
[22] 王强.奶山羊阴外动脉内乳成分前体物理想平衡模式的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2010.
[23] MAXIN G,RULQUIN H,GLASSER F.Response of milk fat concentration and yield to nutrient supply in dairy cows[J].Animal,2011,5(8):1299-1310.  
[24] JACOBS A A A,DIJKSTRA J,LIESMAN J S,et al.Effects of short- and long-chain fatty acids on the expression of stearoyl-CoA desaturase and other lipogenic genes in bovine mammary epithelial cells[J].Animal,2013,7(9):1508-1516.  
[25] SHENG R,YAN S M,QI L Z,et al.Effect of the ratios of acetate and β-hydroxybutyrate on the expression of milk fat- and protein-related genes in bovine mammary epithelial cells[J].Czech Journal of Animal Science,2015,60(12):531-541.  
[26] SHENG R,YAN S M,QI L Z,et al.Effect of the ratios of unsaturated fatty acids on the expressions of genes related to fat and protein in the bovine mammary epithelial cells[J].In Vitro Cellular & Developmental Biology-Animal,2015,51(4):381-389.  
[27] QI L Z,YAN S M,SHENG R,et al.Effects of saturated long-chain fatty acid on mRNA expression of genes associated with milk fat and protein biosynthesis in bovine mammary epithelial cells[J].Asian-Australasian Journal of Animal Sciences,2014,27(3):414-421.  
[28] JACOBS A A A,LIESMAN J S,VAN HAAREN M J,et al.Effects of short- and long-chain fatty acids on expression of lipgenic genes in bovine mammary epithelial cells[C]//2011 Annual Meeting Abstracts.San Francisco,California,USA:ASBMR,2011.
[29] 齐利枝.乳脂前体物及其配比对奶牛乳腺上皮细胞内乳脂肪及乳蛋白合成的影响机理研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2013.
[30] BIONAZ M,LOOR J J.Gene networks driving bovine milk fat synthesis during the lactation cycle[J].BMC Genomics,2008,9:366.
[31] LEHNER R,KUKSIS A.Biosynthesis of triacylglycerols[J].Progress in Lipid Research,1996,35(2):169-201.  
[32] KAPAHI P.Protein synthesis and the antagonistic pleiotropy hypothesis of aging[M]//Tavernarakis N.Protein Metabolism and Homeostasis in Aging.US:Springer,2010:30-37.
[33] DESVERGNE B,MICHALIK L,WAHLI W.Transcriptional regulation of metabolism[J].Physiological Reviews,2006,86(2):465-514.  
[34] 陈智梅.不同氨基酸模式对奶牛α-酪蛋白合成和激素分泌及脂肪合成影响的研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2009.
[35] 何伯萍.调节奶牛乳腺上皮细胞乳脂乳蛋白合成的细胞因子筛选及作用研究[D].硕士学位论文.长春:吉林大学,2013.
[36] LIU X F,LI M,LI Q Z,et al.STAT5A increases lactation of dairy cow mammary gland epithelial cells cultured in vitro[J].In Vitro Cellular & Developmental Biology.Animal,2012,48(9):554-561.  
[37] LAPLANTE M,SABATINI D M.mTOR signaling at a glance[J].Journal of Cell Science,2009,122(20):3589-3594.  
[38] HAYASHI A A,NONES K,ROY N C,et al.Initiation and elongation steps of mRNA translation are involved in the increase in milk protein yield caused by growth hormone administration during lactation[J].Journal of Dairy Science,2009,92(5):1889-1899.  
[39] PROUD C G.Signalling to translation:how signal transduction pathways control the protein synthetic machinery[J].Biochemical Journal,2007,403(2):217-234.  
[40] HORTON J D,GOLDSTEIN J L,BROWN M S.SREBPs:activators of the complete program of cholesterol and fatty acid synthesis in the liver[J].The Journal of Clinical Investigation,2002,109(9):1125-1131.  
[41] BLANCHARD P G,FESTUCCIA W T,HOUDE V P,et al.Major involvement of mTOR in the PPARγ-induced stimulation of adipose tissue lipid uptake and fat accretion[J].The Journal of Lipid Research,2012,53(6):1117-1125.  
[42] LUYIMBAZI D,AKCAKANAT A,MCAULIFFE P F,et al.Rapamycin regulates stearoyl CoA desaturase 1 expression in breast cancer[J].Molecular Cancer Therapeutics,2010,9(10):2770-2784.  
[43] 生冉.乙酸参与奶牛乳腺上皮细胞内乳脂肪与乳蛋白合成的调控机理研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2015.
[44] HAWLEY S A,SELBERT M A,GOLDSTEIN E G,et al.5'-AMP activates the AMP-activated protein kinase cascade,and Ca2+/calmodulin activates the calmodulin-dependent protein kinase Ⅰ cascade,via three independent mechanisms[J].Journal of Biological Chemistry,1995,270(45):27186-27191.  
[45] HONG S P,LEIPER F C,WOODS A,et al.Activation of yeast Snf1 and mammalian AMP-activated protein kinase by upstream kinases[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(15):8839-8843.  
[46] MOMCILOVIC M,HONG S P,CARLSON M.Mammalian TAK1 activates Snf1 protein kinase in yeast and phosphorylates AMP-activated protein kinase in vitro[J].Journal of Biological Chemistry,2006,281(35):25336-25343.  
[47] ROPELLE E R,PAULI J R,FERNANDES M F,et al.A Central Role for Neuronal AMP-Activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) in high-Protein diet-induced weight loss[J].Diabetes,2008,57(3):594-605.  
[48] LI Y,XU S Q,MIHAYLOVA M M,et al.AMPK phosphorylates and inhibits SREBP activity to attenuate hepatic steatosis and atherosclerosis in diet-induced insulin-resistant mice[J].Cell Metabolism,2011,13(4):376-388.  
[49] APPUHAMY J A D R N,NAYANANJALIE W A,ENGLAND E M,et al.Effects of AMP-activated protein kinase (AMPK) signaling and essential amino acids on mammalian target of rapamycin (mTOR) signaling and protein synthesis rates in mammary cells[J].Journal of Dairy Science,2014,97(1):419-429.  
[50] KUDCHODKAR S B,DEL PRETE G Q D,MAGUIRE T G,et al.AMPK-mediated inhibition of mTOR kinase is circumvented during immediate-early times of human cytomegalovirus infection[J].Journal of Virology,2007,81(7):3649-3651.  
[51] 白洁.肉鸡缬氨酸和异亮氨酸的营养需要及其与脂肪代谢的关系[D].硕士学位论文.杭州:浙江大学,2014.
[52] 黄德强,罗凌玉,王丽丽,等.AMPK在胰岛素信号转导通路中的作用[J].中国细胞生物学学报,2011,33(11):1220-1229.
[53] 杨烨,宋娇,付睿琦,等.北京油鸡AMPK基因表达规律及其对肌肉和脂肪细胞内脂肪沉积的影响[J].畜牧兽医学报,2012,43(11):1703-1709.
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