动物营养学报 >

2016 , Vol. 28 >Issue 5: 1384 - 1393

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.05.014

反刍动物营养 Ruminant Nutrition

断奶时间对不同日龄湖羊羔羊瘤胃形态及表皮生长相关基因表达的影响

  • 刘婷 ,
  • 李发弟 ,
  • 李冲 ,
  • 王维民 ,
  • 汪晓娟 ,
  • 李飞 ,
  • 郑琛 ,
  • 莫负涛 ,
  • 王芳彬 ,
  • 喇永富 ,
  • 李宝胜
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  • 1. 甘肃农业大学动物科学技术学院, 兰州 730070;
    2. 甘肃省肉羊繁育生物技术工程实验室, 民勤 733300;
    3. 兰州大学草地农业科技学院, 兰州 730020;
    4. 甘肃金昌中天羊业有限公司, 金昌 737100

收稿日期: 2016-01-12

  网络出版日期: 2016-05-24

基金资助

国家自然科学基金(31260564);国家现代肉羊产业技术体系(CARS-39)

收起

Effects of Weaner Time on Rumen Morphology and Gene Expressions Involved in Rumen Epidermis Growth of Hu Lambs at Different Days of Age

  • LIU Ting ,
  • LI Fadi ,
  • LI Chong ,
  • WANG Weimin ,
  • WANG Xiaojuan ,
  • LI Fei ,
  • ZHENG Chen ,
  • MO Futao ,
  • WANG Fangbin ,
  • LA Yongfu ,
  • LI Baosheng
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  • 1. College of Animal Science and Technology, Gansu Agriculture University, Lanzhou 730070, China;
    2. Engineering Laboratory of Sheep Breeding and Reproduction Biotechnology in Gansu Province, Minqin 733300, China;
    3. College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China;
    4. Jinchang Zhongtian Sheep Industry Co. Ltd., Jinchang 737100, China

Received date: 2016-01-12

  Online published: 2016-05-24

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摘要

本试验旨在探讨断奶时间对不同日龄湖羊羔羊瘤胃形态及表皮生长相关基因表达的影响。采用两因子试验设计,设断奶时间和羔羊日龄2个因子。选择初生重[(3.51±0.57) kg]接近的54只湖羊羔羊,28日龄时随机屠宰6只后按照同质性原则将剩余48只湖羊分为28日龄断奶组[(8.21±0.97) kg]和56日龄断奶组[(8.06±0.53) kg],分别在42、56、70和84日龄从2组中各随机挑选6只羔羊进行屠宰。采集瘤胃腹囊组织样品测定瘤胃乳头长度、宽度和肌层厚度,提取瘤胃组织总RNA测定表皮生长相关基因表达量。结果表明:28日龄断奶组羔羊瘤胃乳头长度和宽度显著高于56日龄断奶组羔羊(P<0.05),瘤胃肌层厚度显著低于56日龄断奶组羔羊(P<0.05)。断奶时间和羔羊日龄之间的交互作用对瘤胃乳头长度和肌层厚度有显著影响(P<0.05)。28日龄断奶组羔羊瘤胃上皮胰岛素样生长因子结合蛋白(IGFBP)3、IGFBP5和IGFBP6表达量显著高于56日龄断奶组(P<0.05)。羔羊瘤胃乳头长度与转化生长因子β(TGFβ)1和IGFBP6表达量呈显著负相关(r=-0.318,P=0.001;r=-0.520,P<0.001);瘤胃乳头宽度与TGFβ1表达量呈显著负相关(r=-0.275,P=0.004),与IGFBP3和IGFBP5表达量呈显著正相关(r=0.344,P<0.001;r=0.256,P=0.001)。综上所述,28日龄断奶促进湖羊羔羊瘤胃乳头发育和瘤胃表皮生长相关基因表达,可能参与羔羊瘤胃早期发育的调控。

关键词： 湖羊羔羊; 早期断奶; 瘤胃形态学; 发育; 基因表达

本文引用格式

刘婷 , 李发弟 , 李冲 , 王维民 , 汪晓娟 , 李飞 , 郑琛 , 莫负涛 , 王芳彬 , 喇永富 , 李宝胜 . 断奶时间对不同日龄湖羊羔羊瘤胃形态及表皮生长相关基因表达的影响[J]. 动物营养学报, 2016 , 28(5) : 1384 -1393 . DOI: 10.3969/j.issn.1006-267x.2016.05.014

Abstract

The objective of this study was to investigate the effects of weaner time on rumen morphology and gene expressions involved in rumen epidermis growth of Hu lambs at different days of age. Two-factor design was used, and the factors were weaner time and days of age of lambs. A total of 54Hu lambs with similar birth weight[(3.51±0.57) kg] were selected, and 6 of them were randomly selected and slaughtered on 28 days of age, while the remaining animals were randomly assigned to a 28 days of age weaner group[(8.21±0.97) kg] or a 56 days of age weaner group[(8.06±0.53) kg]. Six lambs from each group were randomly selected and slaughtered at 42, 56, 70 and 84 days of age, respectively. The ventral sac sample of rumen was collected to measure rumen papilla height, width and muscular thickness, and the tissue sample was extracted total RNA to measure gene expressions involved in rumen epidermis growth. The results showed as follows:rumen papilla height and thickness in 28 days of age weaner group was significantly higher than that in 56 days of age weaner group (P<0.05), however, rumen muscular thickness in 28 days of age weaner group was significantly lower than that in 56 days of age weaner group (P<0.05). There were significant interactions of weaner time and days of age for rumen papilla height and muscular thickness (P<0.05). The expressions of insulin-like growth factor binding protein (IGFBP)3, IGFBP5 and IGFBP6 in 28 days of age weaner group were significantly higher compared with 56 days of age weaner group (P<0.05). Rumen papilla length was negatively correlated with expressions of IGFBP6 and transforming growth factor β (TGFβ)1(r=-0.318, P=0.001; r=-0.520, P<0.001); rumen papilla width was negatively correlated with the expression of TGFβ1 (r=-0.275, P=0.004), while were positively correlated with expressions of IGFBP3 and IGFBP5 (r=0.344, P<0.001; r=0.256, P=0.001). In conclusion, the results indicate that weaning at 28 days of age can promote rumen papillae development, affect expressions of genes involved in rumen epidermis growth, and may modulate early development of rumen of Hu lambs.

Key words： Hu lamb; early weaning; rumen morphology; development; gene expression

参考文献

[1] LESMEISTER K E,HEINRICHS A J, GABLER M T.Effects of supplemental yeast (Saccharomyces cerevisiae) culture on rumen development,growth characteristics,and blood parameters in neonatal dairy calves[J].Journal of Dairy Science,2004,87(6):1832-1839.  
[2] RUSSELL J B,O'CONNOR J D,FOX D G,et al.A net carbohydrate and protein system for evaluating cattle diets:Ⅰ.Ruminal fermentation[J].Journal of Animal Science,1992,70(11):3551-3561.
[3] LESMEISTER K E,HEINRICHS A J.Effects of adding extra molasses to a texturized calf starter on rumen development,growth characteristics,and blood parameters in neonatal dairy calves[J].Journal of Dairy Science,2005,88(1):411-418.  
[4] ŽITÑAN R,VOIGT J,WEGNER J,et al.Morphological and functional development of the rumen in the calf:influence of the time of weaning.1.Morphological development of rumen mucosa[J].Archiv für Tierernaehrung,1999,52(4):351-362.
[5] KLEIN R D,KINCAID R L,HODGSON A S,et al.Dietary fiber and early weaning on growth and rumen development of calves[J].Journal of Dairy Science,1987,70(10):2095-2104.  
[6] 李述信,孟广政,于建国,等.AOAC分析方法手册[M].14版.北京:中国光学学会光谱专业委员会,1986.
[7] 张丽英.饲料分析及饲料质量检测技术[M].3版.北京:中国农业大学出版社,2007.
[8] 中国饲料数据库.中国饲料成分及营养价值表(2013年第24版)(续)[J].中国饲料,2013(22):38-42.
[9] 马志远,李飞,李发弟,等.早期断奶对湖羊羔羊生长性能及胃肠道发育的影响[J].动物营养学报,2015,27(5):1385-1393.
[10] 施恩青.改良与常规石蜡切片方法在切片质量方面的比较[J].齐齐哈尔医学院学报,2014,35(16):2442-2443.
[11] SCHMITTGEN T D,LIVAK K J.Analyzing real-time PCR data by the comparative CT method[J].Nature Protocols,2008,3(6):1101-1108.  
[12] SHI Y G,MASSAGUÉ J.Mechanisms of TGF-β signaling from cell membrane to the nucleus[J].Cell,2003,113(6):685-700.  
[13] KRISHNAN K,ARNONE B,BUCHMAN A.Intestinal growth factors:potential use in the treatment of inflammatory bowel disease and their role in mucosal healing[J].Inflammatory Bowel Diseases,2011,17(1):410-422.  
[14] CONNOR E E,BALDWIN Ⅵ R L,WALKER M P,et al.Transcriptional regulators transforming growth factor-β1 and estrogen-related receptor-α identified as putative mediators of calf rumen epithelial tissue development and function during weaning[J].Journal of Dairy Science,2014,97(7):4193-4207.  
[15] NAEEM A,DRACKLEY J K,LANIER J S,et al.Ruminal epithelium transcriptome dynamics in response to plane of nutrition and age in young Holstein calves[J].Functional & Integrative Genomics,2014,14(1):261-273.  
[16] FIRTH S M,BAXTER R C.Cellular actions of the insulin-like growth factor binding proteins[J].Endocrine Reviews,2002,23(6):824-854.  
[17] SANDERSON I R.Short chain fatty acid regulation of signaling genes expressed by the intestinal epithelium[J].The Journal of Nutrition,2004,134(9):2450S-2454S.
[18] MALHI M,GUI H B,YAO L,et al.Increased papillae growth and enhanced short-chain fatty acid absorption in the rumen of goats are associated with transient increases in cyclin D1 expression after ruminal butyrate infusion[J].Journal of Dairy Science,2013,96(12):7603-7616.  
[19] STEELE M A,CROOM J,KAHLER M,et al.Bovine rumen epithelium undergoes rapid structural adaptations during grain-induced subacute ruminal acidosis[J].American Journal of Physiology Regulatory,Integrative and Comparative Physiology,2011,300(6):R1515-R1523.
[20] BACH L A.IGFBP-6 five years on; not so ‘forgotten’?[J].Growth Hormone & IGF Research,2005,15(3):185-192.  
[21] STEELE M A,SCHIESTEL C,ALZAHAL O,et al.The periparturient period is associated with structural and transcriptomic adaptations of rumen papillae in dairy cattle[J].Journal of Dairy Science,2015,98(4):2583-2595
[22] LESMEISTER K E,TOZER P R,HEINRICHS A J.Development and analysis of a rumen tissue sampling procedure[J].Journal of Dairy Science,2004,87(5):1336-1344.  
[23] GORKA P,KOWALSKI Z M,PIETRZAK P,et al.Effect of sodium butyrate supplementation in milk replacer and starter diet on rumen development in calves[J].Journal of Physiology and Pharmacology,2009,60(Suppl.3):47-53.
[24] REY M,ENJALBERT F, MONTEILS V.Establishment of ruminal enzyme activities and fermentation capacity in dairy calves from birth through weaning[J].Journal of Dairy Science,2012,95(3):1500-1512.  
[25] ANDERSON K L,NAGARAJA T G,MORRILL J L.Ruminal metabolic development in calves weaned conventionally or early[J].Journal of Dairy Science,1987,70(5):1000-1005.  
[26] SUÁREZ B J,VAN REENEN C G,STOCKHOFE N,et al.Effect of roughage source and roughage to concentrate ratio on animal performance and rumen development in veal calves[J].Journal of Dairy Science,2007,90(5):2390-2403.  
[27] BEIRANVAND H,GHORBANI G R,KHORVASH M,et al.Forage and sugar in dairy calves' starter diet and their interaction on performance,weaning age and rumen fermentation[J].Journal of Animal Physiology and Animal Nutrition,2014,98(3):439-445.  
[28] NEMATI M,AMANLOU H,KHORVASH M,et al.Rumen fermentation,blood metabolites,and growth performance of calves during transition from liquid to solid feed:effects of dietary level and particle size of alfalfa hay[J].Journal of Dairy Science,2015,98(10):7131-7141.  
[29] STOBO I J,ROY J H,GASTON H J.Rumen development in the calf.1.The effect of diets containing different proportions of concentrates to hay on rumen development[J].The British Journal of Nutrition,1966,20(2):171-188.  
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