水产营养 Aquaculture Nutrition

饲料中不同维生素D含量对鲈鱼幼鱼生长性能和钙磷代谢的影响

  • 张璐 ,
  • 李静 ,
  • 麦康森 ,
  • 艾庆辉 ,
  • 张春晓 ,
  • 李会涛 ,
  • 袁禹惠
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  • 1. 中国海洋大学水产学院, 教育部海水养殖重点实验室, 青岛 266003;
    2. 通威股份有限公司技术中心, 成都 610041;
    3. 中国石油大学(华东)化工学院, 青岛 266580

收稿日期: 2015-11-13

  网络出版日期: 2016-05-24

基金资助

国家"十五"科技攻关计划项目(2001BA505B-06,2004BA526B06);国家自然科学基金项目(30901108);山东省自然科学基金项目(ZR2009EQ007)

Effects of Different Dietary Vitamin D Contents on Growth Performance, Calcium and Phosphorus Metabolism of Juvenile Japanese Seabass (Lateolabrax japonicas)

  • ZHANG Lu ,
  • LI Jing ,
  • MAI Kangsen ,
  • AI Qinghui ,
  • ZHANG Chunxiao ,
  • LI Huitao ,
  • YUAN Yuhui
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  • 1. The Key Laboratory of Mariculture, Education Ministry of China, Fishery College, Ocean University of China, Qingdao 266003, China;
    2. Technology Center of Tongwei Co., Ltd., Chengdu 610041, China;
    3. Center for Bioengineering and Biotechnology, China University of Petroleum(East China), Qingdao 266580, China

Received date: 2015-11-13

  Online published: 2016-05-24

摘要

本研究旨在探讨饲料中不同维生素D含量对鲈鱼幼鱼生长性能和钙磷代谢的影响。以初始体重为(2.26±0.03) g的鲈鱼幼鱼为试验对象,随机分为6组(每组3个重复,每个重复15尾鱼),饲喂维生素D含量实测值分别为34.2、219.4、393.8、775.9、1534.1和3091.2 IU/kg的等氮等能饲料,进行9周的养殖试验。结果表明:1)当饲料中维生素D含量在34.2~393.8 IU/kg时,鲈鱼的增重率随着饲料中维生素D含量的升高显著上升(P<0.05),但是当饲料中维生素D含量高于393.8 IU/kg时,鲈鱼的增重率变化不显著并且出现平台期(P>0.05)。同样,鲈鱼的特定生长率、饲料效率和蛋白质效率均表现出与增重率相似的变化趋势。2)饲料中维生素D含量显著影响了鱼体粗灰分、钙和磷含量,脊椎骨、鳃盖骨和鳞片粗灰分含量以及脊椎骨钙和磷含量(P<0.05),但对鱼体粗蛋白质、粗脂肪和水分含量没有显著影响(P>0.05)。3)饲料中维生素D含量对鲈鱼血清碱性磷酸酶活性及羟脯氨酸、钙离子和无机磷含量均有显著影响(P<0.05)。4)饲料中维生素D含量显著影响了鲈鱼肝体指数及肝脏脂肪和维生素D含量(P<0.05)。随着饲料中维生素D含量的升高,肝脏维生素D含量呈现升高的趋势,并在饲料中维生素D含量达到1534.1 IU/kg时变化趋于平稳。以增重率为评价指标,采用折线模型分析得出鲈鱼幼鱼获得最大生长时对饲料中维生素D的需求量为431.0 IU/kg饲料。以肝脏维生素D含量为评价指标,采用二次曲线模型分析得出鲈鱼幼鱼获得最大肝脏维生素D沉积量时对饲料中维生素D的需求量为2444.4 IU/kg饲料。

本文引用格式

张璐 , 李静 , 麦康森 , 艾庆辉 , 张春晓 , 李会涛 , 袁禹惠 . 饲料中不同维生素D含量对鲈鱼幼鱼生长性能和钙磷代谢的影响[J]. 动物营养学报, 2016 , 28(5) : 1402 -1411 . DOI: 10.3969/j.issn.1006-267x.2016.05.016

Abstract

A 9-week feeding experiment was conducted to evaluate the effects of different dietary vitamin D contents on growth performance, calcium and phosphorus metabolism of juvenile Japanese seabass (Lateolabrax japonicas). Juvenile Japanese seabass with the initial body weight of (2.26±0.03) g as the experimental animal, and were randomly divided into 6 groups with 3 replicates each group and 15 fish per replicate. Fish in the 6 groups were fed six isonitrogenous and isoenergentic diets with the measured contents of vitamin D were 34.2, 219.4, 393.8, 775.9, 1534.1 and 3091.2 IU/kg, respectively. The results showed as follows:1) weight gain rare (WGR) was significantly increased with increasing dietary vitamin D content from 34.2 to 393.8 IU/kg (P<0.05), and when the dietary vitamin D content more than 393.8 IU/kg, the WGR had no significant change and presented a trend of forgetting (P>0.05). Special growth rate (SGR), feed efficiency ratio (FER) and protein efficiency ratio (PER) showed a similar tendency to WGR. 2) Dietary vitamin D content significantly influenced the contents of ash, calcium and phosphorus in carcass, ash in vertebra, operculum and scale, and calcium and phosphorus in vertebra of juvenile Japanese seabass (P<0.05), but had no significant influences on the contents of crude protein, crude lipid and moisture in carcass (P>0.05). 3) Serum alkaline phosphatase (AKP) activity, and hydroxyproline (HPro), calcium ion (Ca2+) and inorganic phosphorus (Pi) contents were all significantly affected by dietary vitamin D content (P<0.05). 4) Dietary vitamin D content had significant influences on hepatosomatic index, liver lipid and vitamin D contents (P<0.05). Liver vitamin D content showed an increased trend with increasing dietary vitamin D content, and it changed to be steady when dietary vitamin D content up to 1534.1 IU/kg. Broken-line model analysis with the WGR as the appraising index shows that juvenile Japanese seabass require 431.0 IU/kg diet vitamin D for maximal growth. Base on the quadratic curve model analysis with the liver vitamin D content as the appraising index, juvenile Japanese seabass require 2444.4 IU/kg diet vitamin D for maximal liver vitamin D deposition.

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