泛酸干预脂肪甘油三酯脂肪酶和长链脂酰辅酶A合成酶1基因表达对鹅生长和脂类代谢的反向调控

孔敏; 王宝维; 葛文华; 张名爱; 马传兴; 张肖

doi:10.3969/j.issn.1006-267x.2016.05.019

动物营养学报 >

2016 , Vol. 28 >Issue 5: 1433 - 1441

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.05.019

分子营养 Molecular Nutrition

泛酸干预脂肪甘油三酯脂肪酶和长链脂酰辅酶A合成酶1基因表达对鹅生长和脂类代谢的反向调控

孔敏 ,
王宝维 ,
葛文华 ,
张名爱 ,
马传兴 ,
张肖

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1. 青岛农业大学优质水禽研究所, 国家水禽产业技术体系营养与饲料功能研究室, 青岛 266109;
2. 青岛农业大学动物科技学院, 青岛 266109

收稿日期: 2015-12-10

网络出版日期: 2016-05-24

基金资助

国家水禽产业技术体系专项基金(CARS-43-11);山东省良种工程(2014LZ19)

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Pantothenic Acid Intervention of Adipose Triglyceride Lipase and Long-Chain-Fatty-Acid CoA Ligase 1 Gene Expression and Reverse Regulation of Growth and Lipid Metabolism of Geese

KONG Min ,
WANG Baowei ,
GE Wenhua ,
ZHANG Ming ,
MA Chuanxing ,
ZHANG Xiao

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1. Nutrition and Feed Laboratory of China Agriculture Research System, Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China;
2. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2015-12-10

Online published: 2016-05-24

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摘要

本试验通过研究泛酸对5~16周龄五龙鹅肝脏中脂肪甘油三酯脂肪酶(ATGL)和长链脂酰辅酶A合成酶1(ACSL1)基因表达的影响,并分析其与生长性能、屠宰性能、肉品质的相关性,旨在从分子角度确定鹅饲粮中泛酸的适宜添加水平。选择5周龄五龙鹅360只,随机分为6个组,每个组6个重复,每个重复10只鹅。各组饲粮中泛酸添加水平分别为0(对照)、5、10、20、40、80 mg/kg。试验期12周。结果表明:1)随着饲粮泛酸添加水平的提高,ATGL mRNA的表达量呈现先降低后升高的趋势,ACSL1 mRNA表达量呈现先升高后降低的趋势。由回归方程得出,当饲粮泛酸添加水平为13.86 mg/kg时,ATGL mRNA表达量最低;当添加水平为22.07 mg/kg时,ACSL1 mRNA表达量最高。2)与对照组相比,饲粮泛酸添加水平为10~20 mg/kg时极显著提高了五龙鹅的体重和平均日增重(P<0.01),同时极显著降低料重比(P<0.01)。3)ATGL mRNA表达量与胸肌率、腿肌率、屠宰率、半净膛率和全净膛率呈负相关;ACSL1 mRNA表达量与胸肌率、腿肌率、屠宰率、半净膛率和全净膛率呈正相关;二者mRNA表达量与腹脂率均呈负相关。4)ACSL1 mRNA表达量与红度和滴水损失显著相关(P<0.05)。5)ATGL mRNA表达量与血清脂类代谢各项指标呈正相关;ACSL1 mRNA表达量与血清脂类代谢各项指标呈负相关。由此表明,ATGL和ACSL1 mRNA表达量对鹅机体生长速度、屠宰性能和脂类代谢呈同步反向调控机制;从ATGL和ACSL1 mRNA表达量优势分析,建议5~16周龄鹅饲粮中泛酸适宜添加水平为13.86~22.07 mg/kg。

关键词： 泛酸; ATGL; ACSL1; 基因调控; 生长发育; 脂类代谢

本文引用格式

孔敏 , 王宝维 , 葛文华 , 张名爱 , 马传兴 , 张肖 . 泛酸干预脂肪甘油三酯脂肪酶和长链脂酰辅酶A合成酶1基因表达对鹅生长和脂类代谢的反向调控[J]. 动物营养学报, 2016 , 28(5) : 1433 -1441 . DOI: 10.3969/j.issn.1006-267x.2016.05.019

Abstract

This experiment was conducted to study the impact of pantothenic acid on liver adipose triglyceride lipase (ATGL) and long-chain-fatty-acid CoA ligase 1(ACSL1) gene expression of Wulong geese aged from 5 to 16 weeks, to analyzed the correlation of growth performance, slaughter performance and meat quality with ATGL and ACSL1 genes, and to find the appropriate supplemental level of pantothenic acid of geese from the molecular point of view. A total of 360 five-week-old Wulong geese were randomly divided into 6 groups with 6 replicates per group and 10 geese per replicate. Geese in the 6 groups were fed the basal diet supplemented with 0(control), 5, 10, 20, 40 and 80 mg/kg pantothenic acid, respectively. The experiment lasted for 12 weeks. The results showed as follows:1) with the dietary pantothenic acid supplemented level increasing, the ATGL mRNA expression showed a firstly decreased and then increased trend and the ACSL1 mRNA expression showed a firstly increased and then decreased trend. Derived from the regression equation, the ATGL mRNA expression was the lowest when dietary supplemented with 13.86 mg/kg pantothenic acid, and the ACSL1 mRNA expression was the highest when dietary supplemented with 22.07 mg/kg pantothenic acid. 2) Compared with the control group, dietary supplemented with 10 to 20 mg/kg pantothenic acid significantly improved the body weight and average daily gain (P<0.01), and significantly reduced the feed to gain (P<0.01). 3) The ATGL mRNA expression with percentage of breast muscle, percentage of leg muscle, dressed percentage, percentage of half-eviscerated yield and percentage of eviscerated yield was negatively correlated; the ACSL1 mRNA expression with percentage of breast muscle, percentage of leg muscle, dressed percentage, percentage of half-eviscerated yield and percentage of eviscerated yield was positively correlated; and two genes mRNA expression with percentage of abdominal were negatively correlated. 4) The ACSL1 mRNA expression was significantly correlated with red value and water loss (P<0.05). 5) The ATGL mRNA expression was positively correlated with the serum lipid metabolism indicators, and the ACSL1 mRNA expression was negatively correlated with serum lipid metabolism indicators. In conclusion, the ATGL and ACSL1 mRNA expression of geese with body growth speed, slaughter performance and lipid metabolism shows a synchronous reverse regulation mechanism; from ACSL1 mRNA and ATGL mRNA advantage analysis of expression, the dietary appropriate pantothenic acid supplemental level of geese aged from 5 to 16 weeks is 13.86 to 22.07 mg/kg.

Key words： pantothenic acid; ATGL; ACSL1; gene regulation; growth and development; lipid metabolism

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