动物营养学报 >

2016 , Vol. 28 >Issue 5: 1450 - 1461

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.05.021

饲料营养 Feed Science and Technology

饲粮添加壳寡糖和干酪乳杆菌对肉鸡生长性能、肌肉品质及抗氧化性能的影响

  • 李阳 ,
  • 常文环 ,
  • 张姝 ,
  • 郑爱娟 ,
  • 刘国华 ,
  • 蔡辉益 ,
  • 刘伟
展开
  • 农业部饲料生物技术重点开放实验室, 中国农业科学院饲料研究所, 北京 100081

收稿日期: 2015-12-10

  网络出版日期: 2016-05-24

基金资助

中国农业科学院科技创新工程(ASTIP)

收起

Effects of Dietary Chitosan Oligosaccharide and Lactobacillus casei on Growth Performance, Meat Quality and Antioxidant Function of Broilers

  • LI Yang ,
  • CHANG Wenhuan ,
  • ZHANG Shu ,
  • ZHENG Aijuan ,
  • LIU Guohua ,
  • CAI Huiyi ,
  • LIU Wei
Expand
  • Key Laboratory of Feed Biotechnology of Agricultural Ministry, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2015-12-10

  Online published: 2016-05-24

Fold

摘要

本试验旨在研究饲粮中添加壳寡糖与干酪乳杆菌对肉鸡生长性能、肌肉品质及抗氧化性能的影响。选用1日龄爱拔益加(AA)健康肉公鸡240只,随机分为4组,每组6个重复,每个重复10只。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加120 mg/kg壳寡糖、2×106 CFU/g干酪乳杆菌、120 mg/kg壳寡糖+2×106 CFU/g干酪乳杆菌。试验期为42 d。结果表明:1)与对照组相比,单独添加壳寡糖或壳寡糖与干酪乳杆菌共同添加可显著提高肉鸡平均日增重、胸肌和腿肌红度(a*)值,肌肉脂肪和肌苷酸含量以及胸肌单不饱和脂肪酸和多不饱和脂肪酸含量(P<0.05),显著降低胸肌饱和脂肪酸含量、腿肌黄度(b*)值(P<0.05)。2)饲粮中单独添加干酪乳杆菌显著提高腿肌脂肪及胸肌单不饱和脂肪酸含量(P<0.05),显著降低胸肌饱和脂肪酸含量(P<0.05)。3)饲粮中单独添加壳寡糖、干酪乳杆菌或二者共同添加均可显著降低血浆、胸肌和腿肌丙二醛含量(P<0.05),显著提高血浆、胸肌和腿肌总超氧化物歧化酶活性及总抗氧化能力(P<0.05),显著降低血浆肌酸激酶的活性(P<0.05)。综合分析表明,饲粮中添加壳寡糖、干酪乳杆菌或二者共同添加可提高肉鸡的生长性能和抗氧化性能,改善肌肉品质,而单独添加120 mg/kg壳寡糖效果最佳。

关键词： 壳寡糖; 干酪乳杆菌; 肉鸡; 生长性能; 肌肉品质; 抗氧化性能

本文引用格式

李阳 , 常文环 , 张姝 , 郑爱娟 , 刘国华 , 蔡辉益 , 刘伟 . 饲粮添加壳寡糖和干酪乳杆菌对肉鸡生长性能、肌肉品质及抗氧化性能的影响[J]. 动物营养学报, 2016 , 28(5) : 1450 -1461 . DOI: 10.3969/j.issn.1006-267x.2016.05.021

Abstract

This study was conducted to investigate the effects of dietary chitosan oligosaccharide (COS) and Lactobacillus casei on growth performance, meat quality and antioxidant function of broilers. A total of 2401-day-old healthy Arbor Acres male broilers were randomly allotted to 4 groups with 6 replicates per group and 10 birds per replicate. The four diets included a basal diet and 3 experimental diets supplemented with 120 mg/kg COS, 2×106 CFU/g Lactobacillus casei, 120 mg/kg COS+2×106 CFU/g Lactobacillus casei, respectively. The trial lasted for 42 d. The results showed as follows:1) compared with the control group, dietary supplementation with COS or COS and Lactobacillus casei significantly increased average daily gain, redness (a*) value of breast and thigh muscle, contents of intramuscular fat and inosinic acid, and contents of monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) in breast muscle of broilers (P<0.05), significantly decreased saturated fatty acid (SFA) content in breast muscle and yellowness (b*) value of thigh muscle (P<0.05). 2) Supplementation with Lactobacillus casei in diet significantly increased intramuscular fat content in thigh muscle and MUFA content in breast muscle while decreased SFA content in breast muscle (P<0.05). 3) Dietary supplementation with COS, Lactobacillus casei or two together significantly decreased malondialdehyde (MDA) content in plasma, breast and thigh muscle (P<0.05), significantly increased the total superoxide dismutase (T-SOD) activity and total antioxidant capacity (T-AOC) in plasma, breast and thigh muscle (P<0.05), meanwhile decreased the creatine kinase (CK) activity in plasma (P<0.05). The results indicate that dietary supplementation with COS, Lactobacillus casei or both together can increase growth performance and antioxidant function and promote meat quality of broilers, and 120 mg/kg COS supplementation gets the best effect.

Key words： chitosan oligosaccharide; Lactobacillus casei; broiler; growth performance; meat quality; antioxidant function

参考文献

[1] SWIATKIEWICZ S,SWIATKIEWICZ M,ARCZEWSKA-WLOSEK A,et al.Chitosan and its oligosaccharide derivatives (chito-oligosaccharides) as feed supplements in poultry and swine nutrition[J].Journal of Animal Physiology and Animal Nutrition,2015,99(1):1-12.  
[2] 王红卫,孙敏敏,孟晓,等.不同分子质量壳寡糖对蛋鸡生产性能、肠道微生物及脾脏白细胞介素-2和肿瘤坏死因子-α基因表达的影响[J].动物营养学报,2013,25(11):2660-2667.
[3] 李晓晶.壳寡糖对肉仔鸡促生长、免疫调节和抗氧化作用的研究[D].硕士学位论文.北京:中国农业大学,2007.
[4] 刘含亮,孙敏敏,王红卫,等.壳寡糖对虹鳟生长性能、血清生化指标及非特异性免疫功能的影响[J].动物营养学报,2012,24(3):479-486.
[5] 马建爽,常文环,张姝,等.甜菜碱对肉鸡生长性能、脂质代谢及肌肉风味品质的影响[J].动物营养学报,2015,27(1):185-195.
[6] 黄鑫玮,杨莎莎,刘毅,等.壳寡糖对幼建鲤生长性能、脂肪代谢、非特异性免疫功能和肠道健康的影响[J].动物营养学报,2015,27(7):2106-2114.
[7] LIU P,PIAO X S,KIM S W,et al.Effects of chito-oligosaccharide supplementation on the growth performance,nutrient digestibility,intestinal morphology,and fecal shedding of Escherichia coli and Lactobacillus in weaning pigs[J].Journal of Animal Science,2008,86(10):2609-2618.  
[8] ZHOU T X,CHO J H,KIM I H.Effects of supplementation of chito-oligosaccharide on the growth performance,nutrient digestibility,blood characteristics and appearance of diarrhea in weanling pigs[J].Livestock Science,2012,144(3):263-268.  
[9] CHEN Y J,KIM I H,CHO J H,et al.Effects of chitooligosaccharide supplementation on growth performance,nutrient digestibility,blood characteristics and immune responses after lipopolysaccharide challenge in weanling pigs[J].Livestock Science,2009,124(1/2/3):255-260.
[10] TANG Z R,YIN L Y,NYACHOTI C M,et al.Effect of dietary supplementation of chitosan and galacto-mannan-oligosaccharide on serum parameters and the insulin-like growth factor-Ⅰ mRNA expression in early-weaned piglets[J].Domestic Animal Endocrinology,2005,28(4):430-441.  
[11] WANG J P,YOO J S,KIM H J,et al.Nutrient digestibility,blood profiles and fecal microbiota are influenced by chitooligosaccharide supplementation of growing pigs[J].Livestock Science,2009,125(2/3):298-303.
[12] 马现永,蒋宗勇,王志林,等.乳杆菌仔猪肠道黏膜免疫系统的调控及其分子机制[J].动物营养学报,2015,27(8):2317-2322.
[13] 杨艳,潘宝海,孙笑非.植物乳杆菌的功能及在动物生产中的应用[J].饲料研究,2013(2):36-37.
[14] WILLIS W L,ISIKHUEMHEN O S,IBRAHIM S A.Performance assessment of broiler chickens given mushroom extract alone or in combination with probiotics[J].Poultry Science,2007,86(9):1856-1860.  
[15] 县怡涵,赵秀英,李晨博,等.植物乳杆菌和干酪乳杆菌对仔猪生长性能、器官指数及小肠形态的影响[J].动物营养学报,2015,27(12):3805-3811.
[16] LEE H W,PARK Y S,JUNG J S,et al.Chitosan oligosaccharides,dp 2-8,have prebiotic effect on the Bifidobacterium bifidium and Lactobacillus sp.[J].Anaerobe,2002,8(6):319-324.  
[17] 郭元晟.乳酸杆菌对肉鸡生产性能、免疫机能及肠道菌群的影响[D].博士学位论文.呼和浩特:内蒙古农业大学,2011.
[18] 徐基利,许丽.不同乳酸菌及其添加水平对肉仔鸡生长性能、免疫机能和肠道结构的影响[J].动物营养学报,2011,23(11):1976-1983.
[19] 王秀武,林欣,张丽,等.壳寡糖对肉仔鸡生产性能、小肠组织结构和肌组织矿物质元素含量的影响[J].中国粮油学报,2005,20(2):83-88.
[20] HERICH R,RÉVAJOVÁ V M,BOMBA A,et al.The effect of Lactobacillus paracasei and Raftilose P95 upon the non-specific immune response of piglets[J].Food and Agricultural Immunology,2002,14(3):171-179.  
[21] HUANG R L,YIN Y L,WU G Y,et al.Effect of dietary oligochitosan supplementation on ileal digestibility of nutrients and performance in broilers[J].Poultry Science,2005,84(9):1383-1388.  
[22] LI X J,PIAO X S,KIM S W,et al.Effects of chito-oligosaccharide supplementation on performance,nutrient digestibility,and serum composition in broiler chickens[J].Poultry Science,2007,86(6):1107-1114.  
[23] SAFALAOH A C L.Body weight gain,dressing percentage,abdominal fat and serum cholesterol of broilers supplemented with a microbial preparation[J].African Journal of Food Agriculture Nutrition and Development,2006,6(1):1-10.
[24] KALAVATHY R,ABDULLAH N,JALALUDIN S,et al.Effects of Lactobacillus cultures on growth performance,abdominal fat deposition,serum lipids and weight of organs of broiler chickens[J].British Poultry Science,2003,44(1):139-144.  
[25] 徐后国,艾庆辉,麦康森,等.饲料中添加枯草芽孢杆菌和壳寡糖对大黄鱼幼鱼血清免疫指标的影响[J].中国海洋大学学报,2011,41(7/8):42-47.
[26] 徐俊光.壳寡糖对植物病原真菌的抑菌活性及其机理的初步研究[D].博士学位论文.大连:中国科学院研究生院,2007.
[27] 孙建广,张石蕊,谯仕彦,等.发酵乳酸杆菌对生长肥育猪生长性能和肉品质的影响[J].动物营养学报,2010,22(1):132-138.
[28] 李菊,张日俊.益生素对肉仔鸡生长性能、屠体性状及肉品质的影响[J].动物营养学报,2007,19(4):372-378.
[29] ZHOU T X,CHEN Y J,YOO J S,et al.Effects of chitooligosaccharide supplementation on performance,blood characteristics,relative organ weight,and meat quality in broiler chickens[J].Poultry Science,2009,88(3):593-600.  
[30] 崔焕先.肉鸡肌内脂肪形成的分子调控网络及相关基因研究[D].博士学位论文.北京:中国农业科学院,2011.
[31] 刘羽,冯琳,陈岗富,等.饲料中添加乳酸杆菌对幼建鲤生长性能和消化吸收功能的影响[J].动物营养学报,2011,23(8):1386-1393.
[32] 胡忠泽,王立克,周正奎,等.杜仲对鸡肉品质的影响及作用机理探讨[J].动物营养学报,2006,18(1):49-54.
[33] BRENNER R R.Factors influencing fatty acid chain elongation and desaturation[M]//VERGROESEN A J,CRAWFORD M.The Role of Fats in Human Nutrition.London:Academic Press,1989:45-79.
[34] 黄风华,郑新民,张元珍,等.诊断超声波照射对大鼠睾丸总抗氧化能力、丙二醛及脂质过氧化物酶水平和生殖细胞凋亡的影响[J].武汉大学学报:医学版,2007,28(1):85-88.
[35] 龙次民,谢春艳,吴信,等.妊娠后期母猪饲粮中添加壳寡糖对新生仔猪抗氧化能力的影响[J].动物营养学报,2015,27(4):1207-1213.
[36] 张江巍,曹郁生.乳酸菌抗氧化活性的研究进展[J].中国乳品工业,2005,33(1):34-37.
[37] 李维,孙开济,孙玉丽,等.乳酸菌缓解肠道氧化应激研究进展[J].动物营养学报,2016,28(1):9-14.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2