动物营养学报 >

2016 , Vol. 28 >Issue 5: 1558 - 1565

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.05.033

研究简报 Short Communications

苜草素对蛋鸡胆固醇代谢的影响及其基因调控机制

  • 李宁 ,
  • 刘鑫 ,
  • 曲正祥 ,
  • 刘通 ,
  • 杜宇 ,
  • 高玉鹏 ,
  • 闵育娜
展开
  • 西北农林科技大学动物科技学院, 杨凌 712100

收稿日期: 2015-11-19

  网络出版日期: 2016-05-24

基金资助

现代农业产业技术体系(CARS-41-K13);科技部农业科技成果转化项目(2013GB2G000468);陕西省科技资源统筹项目(2016KTCL02~18)

收起

Effects and Gene Regulatory Mechanism of Polysavone on Cholesterol Metabolism of Laying Hens

  • LI Ning ,
  • LIU Xin ,
  • QU Zhengxiang ,
  • LIU Tong ,
  • DU Yu ,
  • GAO Yupeng ,
  • MIN Yuna
Expand
  • College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China

Received date: 2015-11-19

  Online published: 2016-05-24

Fold

摘要

本试验旨在研究饲粮中添加不同水平苜草素对蛋鸡胆固醇代谢的影响,并探讨其基因表达调控机制。选取体重和产蛋率相近的26周龄尼克蛋鸡540只,随机分为5组,每组6个重复,每个重复18只。对照组饲喂基础饲粮,试验组分别在基础饲粮中添加300、600、900、1200 mg/kg的苜草素。预试期7 d,正试期70 d。结果表明:1)试验第35天和第70天,900 mg/kg苜草素组的蛋黄胆固醇含量显著低于对照组(P<0.05)。2)与对照组相比,900 mg/kg苜草素组显著升高了蛋鸡血清中高密度脂蛋白胆固醇(HDL-C)含量和高密度脂蛋白/低密度脂蛋白(HDL/LDL)(P<0.05),各组蛋鸡血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)含量差异均不显著(P>0.05)。3)与对照组相比,600、900、1200 mg/kg苜草素组显著降低了蛋鸡肝脏中3-羟基-3-甲基戊二酸单酰辅酶A还原酶(HMGCR)mRNA表达量(P<0.05),900和1200 mg/kg苜草素组显著升高了蛋鸡肝脏组织中胆固醇7α-羟化酶(CYP7A1)mRNA表达量(P<0.05)。各组蛋鸡肝脏中固醇结合蛋白元件-2(SERBP-2)和卵巢中卵黄蛋白原受体(OVR)mRNA表达量无显著差异(P>0.05)。综上,饲粮中添加苜草素降低了蛋黄胆固醇和全蛋胆固醇含量,其调控机制可能是通过抑制蛋鸡胆固醇的内源性合成和促进胆固醇向胆汁酸的转化排泄2种途径来实现。本试验推荐26~35周龄蛋鸡苜草素添加水平为900 mg/kg。

关键词： 苜草素; 饲养; 蛋鸡; 胆固醇代谢; 基因表达

本文引用格式

李宁 , 刘鑫 , 曲正祥 , 刘通 , 杜宇 , 高玉鹏 , 闵育娜 . 苜草素对蛋鸡胆固醇代谢的影响及其基因调控机制[J]. 动物营养学报, 2016 , 28(5) : 1558 -1565 . DOI: 10.3969/j.issn.1006-267x.2016.05.033

Abstract

The experiment was carried out to evaluate the effects of dietary different polysavone supplemental level on cholesterol metabolism of laying hens, and discussed its regulation mechanism of gene expression. A total of 540 twenty-six-week-old Nick laying hens were randomly divided into 5 groups with 6 replicates per group and 18 birds per replicate. Laying hens in control group were fed with a basal diet, and other were fed with the basal diets supplemented with 300, 600, 900 and 1200 mg/kg polysavone, respectively. The pre-test lasted for 7 days, and the experiment lasted for 70 days. The results showed as follows:1) on day 35 and day 70, the yolk cholesterol content in 900 mg/kg polysavone group was significantly lower than that in control group (P<0.05). 2) Compared with the control group, the high density lipoprotein-cholesterol (HDL-C) content and high density lipoprotein to low density lipoprotein ratio (HDL/LDL) in 900 mg/kg polysavone group significantly increased (P<0.05). There were no significant differences in the contents of total cholesterol (TC), triglyceride (TG) and low density lipoprotein-cholesterol (LDL-C) in serum among all groups (P>0.05). 3) Compared with the control group, the mRNA expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) in liver of laying hens in 600, 900 and 1200 mg/kg polysavone groups significantly decreased (P<0.05), and the mRNA expression of cholesterol 7α-hydroxylase (CYP7A1) in liver of laying hens in 600 and 900 mg/kg polysavone groups significantly increased (P<0.05). There were no significant differences in the mRNA expression of sterol regulatory element-binding protein 2(SREBP-2) in liver and occyte vitellogenesis receptor (OVR) in ovarian of laying hens among all groups (P>0.05). Our present study indicates that dietary supplemented with polysavone can reduce the whole egg and egg yolk cholesterol content. The possible mechanism of cholesterol lowering effects can result from reducing endogenous synthesis and more cholesterol excretion. The suitable dietary polysavone supplemented level of laying hens aged from 26 to 35 weeks is 900 mg/kg.

Key words： polysavone; feeding; laying hens; cholesterol metabolism; gene expression

参考文献

[1] SPENCE J D,JENKINS D J A,DAVIGNON J.Egg yolk consumption and carotid plaque[J].Atherosclerosis,2012,1-5.
[2] SPENCE J D,JUDD S,HOWARD V,et al.Effect of dietary cholesterol and egg consumption on mortality and cardiovascular risk in the regards study[J].Stroke,2015,46:A83-A83.
[3] ATTIA Y A,AL-HARTHI M A,KORISH M A,et al.Fatty acid and cholesterol profiles and hypocholesterolemic, atherogenic, and thrombogenic indices of table eggs in the retail market[J].Lipids in Health and Disease,2015,14(1):1.
[4] FERNANDEZ M L.Effects of eggs on plasma lipoproteins in healthy populations[J].Food&Function,2010,1(2):156-160.
[5] CHOI Y,CHANG Y,LEE J E,et al.Egg consumption and coronary artery calcification in asymptomatic men and women[J].Atherosclerosis,2015,241(2):305-312.  
[6] Dietary Guidelines Advisory Committee.Scientific report of the 2015 dietary guidelines advisory committee[C].Washington,D.C.:USDA and US Department of Health and Human Services,2015.
[7] CHOI Y,CHANG Y,LEE J E,et al.Egg consumption and coronary artery calcification in asymptomatic men and women[J].Atherosclerosis,2015,241(2):305-312.  
[8] SHIN J Y,XUN P,NAKAMURA Y,et al.Egg consumption in relation to risk of cardiovascular disease and diabetes:a systematic review and meta-analysis[J].The American Journal of Clinical Nutrition,2013:ajcn-051318.
[9] ZAMPELAS A.Still questioning the association between egg consumption and the risk of cardiovascular diseases[J].Atherosclerosis,2012,224(2):318-319.  
[10] GUO J,LOVEGROVE J A,COCKCROFT J R,et al.Egg consumption and cardiovascular disease events-evidence from the Caerphilly prospective cohort study[J].Proceedings of the Nutrition Society,2015,74(OCE5):E291.
[11] SHIN J Y,XUN P,NAKAMURA Y,et al.Egg consumption in relation to risk of cardiovascular disease and diabetes:a systematic review and meta-analysis[J].The American Journal of Clinical Nutrition,2013,98:146-159.
[12] HOUSTON D K,DING J,LEE J S,et al.Dietary fat and cholesterol and risk of cardiovascular disease in older adults:the health ABC study[J].Nutrition Metabolism and Cardiovascular Diseases,2011,21(6):430-437.  
[13] 石传林,张娜,李有志,等.降低鸡蛋中胆固醇含量的试验研究[J].家禽科学,2015(1):48-50.
[14] 佟建明,萨仁娜,单之玮,等.苜草素对肉仔鸡和仔猪生产性能的影响[J].中国畜牧兽医,2004,31(3):19-21.
[15] 雷祖玉,韩卫涛,冯学勤,等.苜蓿总甙对AA肉仔鸡脂类代谢及生产性能的影响[J].中国饲料,2002,18(9):10.
[16] MING M,GUANHUA L,ZHANHAI Y,et al.Effect of the Lycium barbarum polysaccharides administration on blood lipid metabolism and oxidative stress of mice fed high-fat diet in vivo[J].Food Chemistry,2009,113(4):872-877.  
[17] ØRGAARD A,JENSEN L.The effects of soy isoflavones on obesity[J]. Experimental Biology and Medicine,2008,233(9):1066-1080.  
[18] DONG X F,GAO W W,SU J L,et al.Effects of dietary polysavone (Alfalfa extract) and chlortetracycline supplementation on antioxidation and meat quality in broiler chickens[J].British Poultry Science,2011,52(3):302-309.  
[19] 王长康,刘昊,邵良平.苜草素对蛋鸡生产性能和血液生化指标的影响[J].福建农业学报,2006,3:215-218.
[20] 张丽娜,董晓芳,佟建明,等.苜草素对58-65周龄蛋鸡胆固醇和脂类代谢的影响[J].中国家禽,2010,32(12):27-30.
[21] 张旭,胡艳,蒋桂韬,等.蛋黄中胆固醇含量测定方法的研究[J].饲料博览,2011(12):35-38.
[22] 尹靖东,齐广海,张萍,等.大豆黄酮对鸡蛋胆固醇及其耐氧化性的影响[J].中国农业科学,2004,37(5):756-761.
[23] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Methods,2001,25(4):402-408.  
[24] SPENCE J D,JENKINS D J A,DAVIGNON J.Egg yolk consumption and carotid plaque[J].Atherosclerosis,2012,224(2):469-473.  
[25] 陈冬梅,张克英.鸡蛋胆固醇的沉积机制[J].动物营养学报,2003,15(4):1-6.
[26] SIRTORI C R,LOVATI M R,MANZONI C,et al.Reduction of serum cholesterol by soy proteins:clinical experience and potential molecular mechanisms[J].Nutrition,Metabolism and Cardiovascular Diseases,1998,8:334-340.
[27] MALINOW M R,MCLAUGHLIN P,PAPWORTH L,et al.Effect of alfalfa saponins on intestinal cholesterol absorption in rats.[J].The American Journal of Clinical Nutrition,1977,30(12):2061-2067.
[28] 侯永刚,陈辉,黄仁录,等.日粮添加苜蓿皂苷对蛋鸡生产性能,鸡蛋胆固醇及常规蛋品质的影响[J].中国家禽,2009(8):20-23.
[29] 董晓芳,江勇,高微微,等.苜草素对肉仔鸡免疫,内分泌和脂类代谢的一些指标的影响[J].动物营养学报,2007,19(4):407-410.
[30] 谢泰华.苜草素对蛋鸡生产性能、蛋品质和血脂指标的影响[D].硕士学位论文,福州:福建农林大学,2009.
[31] XU X,HU Y,XIAO W,et al.Effects of fermented Camilla sinensis,Fuzhuan tea,on egg cholesterol and production performance in laying hens[J].Agriculture and Food Science,2012,1:6-10.
[32] WANG J J,PAN T M.Effect of red mold rice supplements on serum and egg yolk cholesterol levels of laying hens[J].Journal of Agricultural and Food Chemistry,2003,51(16):4824-4829.  
[33] JUREVICS H,HOSTETTLER J,BARRETT C,et al.Diurnal and dietary-induced changes in cholesterol synthesis correlate with levels of mRNA for HMG-CoA reductase[J].Journal of Lipid Research,2000,41(7):1048-1053.
[34] ELKIN R G,FREED M B,KIEFT,et al.Alteration of egg yolk cholesterol content and plasma lipoprotein profile following administration of a totally synthetic HMG-CoA reductase inhibitor to laying hens[J].Journal of Agricultural and Food Chemistry,1993,41:1094-1101.
[35] 柳童斐,宋保亮.胆固醇合成途径的负反馈调控机制[J].中国细胞生物学学报,2013,35(4):401-409.
[36] SHI Y H,GUO R,WANG X K,et al.The regulation of alfalfa saponin extract on key genes involved in hepatic cholesterol metabolism in hyperlipidemic rats[J].PLoS One,2014,9(2):e88282.
[37] NIMPF J,SCHNEIDER W J.Receptor-mediated lipoprotein transport in laying hens[J].The Journal of Nutrition,1991,121:1471-1474.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2