水产营养 Aquaculture Nutrition

中性植酸酶部分替代磷酸二氢钙对异育银鲫生长性能、体成分及磷利用率的影响

  • 曾晓霭 ,
  • 徐树德 ,
  • 陈清华 ,
  • 轩岩俊 ,
  • 赵青海
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  • 1. 湖南农业大学动物科学技术学院, 长沙 410128;
    2. 广东溢多利生物科技股份有限公司, 珠海 519060

收稿日期: 2015-12-17

  网络出版日期: 2016-06-27

基金资助

"十二五"农村领域国家科技计划课题——新型饲料用酶制剂创制与应用及安全高效饲料添加剂创制与应用(2013BAD10B00);湖南农业大学技术开发合作项目——酶制剂应用效果评估技术体系的研究(13118)

Effects of Partial Replacing Monocalcium Phosphate with Neutral Phytase on Growth Performance, Body Composition and Phosphorus Utilization of Carassius auratus gibelio

  • ZENG Xiaoai ,
  • XU Shude ,
  • CHEN Qinghua ,
  • XUAN Yanjun ,
  • ZHAO Qinghai
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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Guangdong VTR Co., Ltd., Zhuhai 519060, China

Received date: 2015-12-17

  Online published: 2016-06-27

摘要

本试验旨在研究中性植酸酶部分替代磷酸二氢钙对异育银鲫生长性能、体成分、血清生化指标及磷利用率的影响。试验分为7组,分别为D1组(正对照组,饲料中添加1.5%磷酸二氢钙)、D2组(负对照1组,饲料中添加1.0%磷酸二氢钙)、D3组(饲料中添加1.0%磷酸二氢钙+400 U/kg中性植酸酶)、D4组(饲料中添加1.0%磷酸二氢钙+800 U/kg中性植酸酶)、D5组(负对照2组,饲料中添加0.5%磷酸二氢钙)、D6组(饲料中添加0.5%磷酸二氢钙+400 U/kg中性植酸酶)和D7组(饲料中添加0.5%磷酸二氢钙+800 U/kg中性植酸酶),每组3个重复,每个重复25尾鱼[初始均重(23.39±0.10) g],开展为期8周的生长试验。结果表明:异育银鲫摄食试验饲料8周后,D5组的生长性能最差,其增重率、特定生长率、摄食量均显著低于其余各组(P<0.05),饲料系数则显著高于其余各组(P<0.05)。D2和D6组的增重率和特定生长率显著低于D1组(P<0.05),而饲料系数则显著高于D1组(P<0.05)。D3、D4和D7组的各项生长性能指标与D1组均没有显著差异(P>0.05)。异育银鲫的全鱼水分、粗蛋白质、粗灰分和磷含量以及血清胆固醇含量和碱性磷酸酶活性在各组间均无显著差异(P>0.05)。除D5组外,其余各组间血清中磷和甘油三酯含量无显著差异(P>0.05)。在相同的磷酸二氢钙添加量下,添加中性植酸酶组磷沉积率和磷表观消化率显著高于未添加中性植酸酶组(P<0.05),而磷排放量则相反。由此可知,饲料中添加400和800 U/kg中性植酸酶可以分别减少0.5%和1.0%的磷酸二氢钙添加量而不影响异育银鲫的生长性能、体成分和血清生化指标等。在异育银鲫饲料中,中性植酸酶部分替代磷酸二氢钙可以提高饲料磷利用率、降低磷排放量,从而带来经济效益和生态效益。

本文引用格式

曾晓霭 , 徐树德 , 陈清华 , 轩岩俊 , 赵青海 . 中性植酸酶部分替代磷酸二氢钙对异育银鲫生长性能、体成分及磷利用率的影响[J]. 动物营养学报, 2016 , 28(6) : 1710 -1719 . DOI: 10.3969/j.issn.1006-267x.2016.06.011

Abstract

An 8-week feeding trial was conducted to study the effects of partial replacing monocalcium phosphate (MCP) with neutral phytase on growth performance, body composition, serum biochemical parameters and phosphorus utilization of Carassius auratus gibelio. Seven groups were designed in this experiment, there were D1 (the positive control group, 1.5% MCP), D2 (the negative control group 1, 1.0% MCP), D3 (1.0% MCP+400 U/kg neutral phytase), D4 (1.0% MCP+800 U/kg neutral phytase), D5 (the negative control group 2, 0.5% MCP), D6 (0.5% MCP+400 U/kg neutral phytase) and D7 groups (0.5% MCP+800 U/kg neutral phytase). Each group had 3 replicates and each replicate had 25 fish with the initial average body weight of (23.39±0.10) g. The results showed as follows: after the 8-week feeding trial, the D5 group showed the worst growth performance, and the weight gain rate, specific growth rate and feed intake in D5 group were significantly lower than those in the D1 group (P<0.05), while the feed conversion ratio was significantly higher than those in the other groups (P<0.05). The weight gain rate and specific growth rate in D2 and D6 groups were significantly lower than those in D1 group (P<0.05), while the feed conversion ratio was significantly higher than that in the D1 group (P<0.05). All growth performance indices in D3, D4 and D7 groups had no significant differences compared with D1 group (P>0.05). The moisture, crude protein, ash and phosphorus contents of whole body, and serum cholesterol content and alkaline phosphatase activity had no significant differences among the all groups (P>0.05). Serum phosphorus and triglyceride contents, in addition to the D5 group, showed no significant difference between the rest groups (P>0.05). At the same supplemental level of MCP, the phosphorus retention ratio and apparent digestibility in neutral phytase supplementation groups were significantly higher than those in no neutral phytase supplementation groups (P<0.05), while the phosphorus discharge was opposite (P<0.05). The present study suggests that when the diet is supplemented 400 and 800 U/kg of phytase, the MCP level can be reduced by 0.5% and 1.0%, respectively, without affecting the growth performance, body composition and serum biochemical parameters of Carassius auratus gibelio. Neutral phytase partial replacing MCP in Carassius auratus gibelio diet can improve the utilization of phosphorus and reduce the phosphorus discharge, thus brings economical and ecological benefits.

参考文献

[1] NRC.Nutrient requirements of fish[S].Washington,D.C.:National Academy Press,1993.
[2] JACKSON L S,LI M H,ROBINSON E H.Use of microbial phytase in channel catfish Ictalurus punctatus diets to improve utilization of phytate phosphorus[J].Journal of the World Aquaculture Society,1996,27(3):309-313.  
[3] KUMAR V,SINHA A K,MAKKAR H P S,et al.Phytate and phytase in fish nutrition[J].Animal Physiology and Animal Nutrition,2012,96(3):335-364.  
[4] LIEBERT F,PORTZ L.Nutrient utilization of Nile tilapia Oreochromis niloticus fed plant based low phosphorus diets supplemented with graded levels of different sources of microbial phytase[J].Aquaculture,2005,248(1/2/3/4):111-119.
[5] LI M H,ROBINSON E H.Microbial phytase can replace inorganic phosphorus supplements in channel catfish Ictalurus punctatus diets[J].Journal of the World Aquaculture Society,1997,28(4):402-406.  
[6] ROBINSONE H,LI M H,MANNING B B.Comparison of microbial phytase and dicalcium phosphate for growth and bone mineralization of pond-raised channel catfish,Ictalurus punctatus[J].Journal of Applied Aquaculture,2002,12(3):81-88.  
[7] PORTZ L,LIEBERT F.Growth,nutrient utilization and parameters of mineral metabolism in Nile tilapia Oreochromis niloticus (Linneaus,1758) fed plant-based diets with graded levels of microbial phytase[J].Journal of Animal Physiology and Animal Nutrition,2004,88(9/10):311-320.
[8] PHROMKUNTHONG W,GABAUDAN J.Used of microbial phytase to replace inorganic phosphorus in sex-reversed red tilapia:1. Dose response[J].Songklanakarin Journal of Science and Technology,2006,28(4):731-743.
[9] XUE M,CUI Y B.Effect of several feeding stimulants on diet preference by juvenile gibel carp (Carassius auratus gibelio),fed diets with or without partial replacement of fish meal by meat and bone meal[J].Aquaculture,2001,198(3/4):281-292.
[10] ZOU Z,CUI Y,GUI J,et al.Growth and feed utilization in two strains of gibel carp,Carassius auratus gibelio:paternal effects in a gynogenetic fish[J].Journal of Applied Ichthyology,2001,17(2):54-58.  
[11] 仇明,王爱民,吕林兰,等.中性植酸酶对异育银鲫鱼种生长的影响及其在肠道中酶活分布状况[J].粮食与饲料工业,2010(2):38-41.
[12] CARLSSON N G,BERGMAN E L,SKOGLUND E,et al.Rapid analysis of inositol phosphates[J].Journal of Agricultural and Food Chemistry,2001,49(4):1695-1701.  
[13] AOAC.Official methods of analysis of AOAC international[S].16th ed.Arlington,VA:AOAC,1995.
[14] LAINING A,ISHIKAWA M,KOSHIO S,et al.Dietary inorganic phosphorus or microbial phytase supplementation improves growth,nutrient utilization and phosphorus mineralization of juvenile red sea bream,Pagrus major,fed soybean-based diets[J].Aquaculture Nutrition,2012,18(5):502-511.  
[15] CAO L,WANG W M,YANG C T,et al.Application of microbial phytase in fish feed[J].Enzyme and Microbial Technology,2007,40(4):497-507.  
[16] 余丰年,王道尊.植酸酶对异育银鲫生长及饲料中磷利用率的影响[J].中国水产科学,2000,7(2):106-109.
[17] HALVER J E,HARDY R W.Fish nutrition[M].3rd ed.Amsterdam:Academic Press,2002.
[18] YOO G Y,WANG X J,CHOI S,et al.Dietary microbial phytase increased the phosphorus digestibility in juvenile Korean rockfish Sebastes schlegeli fed diets containing soybean meal[J].Aquaculture,2005,243(1/2/3/4):315-332.
[19] LIU L W,SU J M,LUO Y L.Effect of partial replacement of dietary monocalcium phosphate with neutral phytase on growth performance and phosphorus digestibility in gibel carp,Carassius auratus gibelio (Bloch)[J].Aquaculture Research,2012,43(9):1404-1413.  
[20] BAEVERFJORD G,ASGARD T,SHEARER K D.Development and detection of phosphorus deficiency in Atlantic salmon,Salmo salar L.,parr and post-smolts[J].Aquaculture Nutrition,1998,4(1):1-11.  
[21] YE C X,LIU Y J,TIAN L X,et al.Effect of dietary calcium and phosphorus on growth,feed efficiency,mineral content and body composition of juvenile grouper,Epinephelus coioides[J].Aquaculture,2006,255(1/2/3/4):263-271.
[22] SKONBERG D I,YOGEV L,HARDY R W,et al.Metabolic response to dietary phosphorus intake in rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,1997,157(1/2):11-24.
[23] 王秀云,何振平,刘艳芳.植酸酶在水产养殖中的应用(综述)[J].河北科技师范学院学报,2008,22(2):71-75.
[24] 郑涛,潘庆,李桂峰,等.无鱼粉饲料中添加磷和植酸酶对奥尼罗非鱼生长性能及体成分的影响[J].中国水产科学,2006,13(1):112-118.
[25] 李萌,王桂芹.植酸酶对黄金鲫钙磷代谢的影响[J].中国饲料,2015(8):34-38.
[26] SUGIURA S H,RABOY V,YOUNG K A,et al.Availability of phosphorus and trace elements in low-phytate varieties of barley and corn for rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,1999,170(3/4):285-296.
[27] SAJJADI M,CARTER C G.Effect of phytic acid and phytase on feed intake,growth,digestibility and trypsin activity in Atlantic salmon (Salmo salar,L.)[J].Aquaculture Nutrition,2004,10(2):135-142.  
[28] 徐树德,王树启,游翠红,等.中性植酸酶替代磷酸二氢钙对黑鲷生长和磷利用的影响[J].中国水产科学,2014,21(3):522-530.
[29] 杨雨虹,郭庆,祖立闯,等.植酸酶对鲤鱼生长及磷利用率的影响[J].淡水渔业,2006,36(5):20-23.
[30] LANARI D,D'AGARO E,TURRI C.Use of nonlinear regression to evaluate the effects of phytase enzyme treatment of plant protein diets for rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,1998,161(1/2/3/4):345-356.
[31] FORSTER I,HIGGS D A,DOSANJH B S,et al.Potential for dietary phytase to improve the nutritive value of canola protein concentrate and decrease phosphorus output in rainbow trout (Oncorhynchus mykiss) held in 11℃ fresh water[J].Aquaculture,1999,179(1/2/3/4):109-125.
[32] VIELMA J,RUOHONEN K,PEISKER M.Dephytinization of two soy proteins increases phosphorus and protein utilization by rainbow trout,Oncorhynchus mykiss[J].Aquaculture,2002,204(1/2):145-156.
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