动物营养学报 >

2016 , Vol. 28 >Issue 6: 1823 - 1831

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.06.025

研究简报 Short Communications

沙葱总黄酮对肉羊抗氧化能力的影响

  • 木其尔 ,
  • 敖长金 ,
  • 萨茹丽 ,
  • 王特日格乐 ,
  • 陈仁伟 ,
  • 特木其乐 ,
  • 王翠芳
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  • 内蒙古农业大学动物科学学院, 呼和浩特 010018

收稿日期: 2016-03-24

  网络出版日期: 2016-06-27

基金资助

国家自然科学基金地区科学基金项目(31260558,31160474);"十二五"国家科技支撑计划(2013BDA10B04)

收起

Effects of Flavonoids from Allium mongolicum Regel on Antioxidant Capacity of Meat Sheep

  • MU Qier ,
  • AO Changjin ,
  • SA Ruli ,
  • WANG Terigele ,
  • CHEN Renwei ,
  • TE Muqile ,
  • WANG Cuifang
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  • College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2016-03-24

  Online published: 2016-06-27

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摘要

本试验旨在研究基础饲粮中添加沙葱总黄酮对肉羊体内总抗氧化能力(T-AOC)、总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活力以及丙二醛(MDA)含量的影响,以阐明沙葱总黄酮对肉羊的抗氧化作用,并确定沙葱总黄酮在肉羊饲粮中的最适添加量。试验选用60只6月龄左右、体重(39.9±3.2) kg的小尾寒羊为试验动物,按照出生月龄和体重相近的原则,随机分为4组,每组15只羊。对照组饲喂基础饲粮,3个试验组分别在基础饲粮中添加11、22、33 mg/kg的沙葱总黄酮。预试期为15 d,正试期为60 d,试验期第0、15、30、45、60天时空腹颈静脉采血,分离血清,试验期结束后每组分别随机选取3只羊,进行屠宰,取肝脏和脾脏样品,分别测定血清及组织抗氧化指标。结果发现:1)饲粮添加11~33 mg/kg沙葱总黄酮可显著提高血清(第45天开始)及肝脏T-AOC(P<0.05),而对脾脏T-AOC无显著影响(P>0.05)。2)饲粮添加11~33 mg/kg沙葱总黄酮对血清、肝脏及脾脏T-SOD活力,血清CAT活力,血清、脾脏GSH-PX活力有一定的提高,其中33 mg/kg沙葱总黄酮的作用最强,血清中第30天后效果明显;而对肝脏、脾脏CAT活力,肝脏GSH-PX活力均无显著影响(P>0.05)。3)饲粮添加11~33 mg/kg沙葱总黄酮可降低血清、肝脏MDA含量,血清MDA含量第15天后效果明显,对脾脏MDA含量无显著影响(P>0.05)。结果提示,饲粮添加11~33 mg/kg沙葱总黄酮可显著提高肉羊体内抗氧化指标,并其效果具有时间和剂量依赖性,饲喂30 d后沙葱总黄酮开始发挥其体内抗氧化能力。

关键词： 沙葱; 黄酮; 肉羊; 抗氧化能力

本文引用格式

木其尔 , 敖长金 , 萨茹丽 , 王特日格乐 , 陈仁伟 , 特木其乐 , 王翠芳 . 沙葱总黄酮对肉羊抗氧化能力的影响[J]. 动物营养学报, 2016 , 28(6) : 1823 -1831 . DOI: 10.3969/j.issn.1006-267x.2016.06.025

Abstract

This experiment was conducted to investigate the effects of dietary supplementation of flavonoids from Allium mongolicum Regel on total antioxidant capacity (T-AOC), the activities of total superoxide dismutase (T-SOD), catalase (CAT) and glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) content of meat sheep to determine the optimal level. Sixty 6-month-old meat sheep with similar body weight [(39.9±3.2) kg] were randomly assigned to 4 groups with 15 sheep per group. Control group was fed a basal diet, and the 3 experimental groups were fed the basal diet supplemented with 11, 22 and 33 mg/kg flavonoids from Allium mongolicum Regel, respectively. Preliminary feeding period was 15 d, and the experimental period was 60 d. Blood was collected at days 0, 15, 30, 45, 60 via jugular venipuncture, and serum was harvested. Three sheep were randomly selected from each of the four groups for slaughter after experiment, and samples of liver and spleen were collected to determine the antioxidant indices. The results showed as follows: 1) dietary supplementation of 11 to 33 mg/kg flavoniods from Allium mongolicum Regel significantly increased T-AOC in serum (from day 45) and liver (P<0.05), but had no significant effect on that in spleen (P>0.05). 2)Dietary supplementation of 11 to 33 mg/kg flavoniods from Allium mongolicum Regel had positive effect on the activities of T-SOD in serum, liver and spleen, CAT in serum, and GSH-PX in serum and spleen; the supplemental level of 33 mg/kg showed the highest effects, and the effects became obvious after day 30 in serum; however, it had no significant effects on CAT in liver and spleen, and GSH-PX in liver (P>0.05). 3) Dietary supplementation of 11 to 33 mg/kg flavoniods from Allium mongolicum Regel could decrease the content of MDA in serum and liver, and the effects became obvious after day 15 in serum, however, it had no significant effects on the content of MDA in spleen (P>0.05). The results indicate that dietary supplementation of 11 to 33 mg/kg flavoniods from Allium mongolicum Regel can significantly improve antioxidant capacity of meat sheep, and the antioxidant capacity is in time-dependent and dose-dependent manners. Flavoniods from Allium mongolicum Regel begin to play its role after fed for 30 d.

Key words： Allium mongolicum Regel; flavoniods; meat sheep; antioxidant capacity

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