不同无机磷水平饲粮添加植酸酶对断奶仔猪骨骼钙磷代谢的影响

王德海; 杨维仁; 郭宝林; 张崇玉; 郭文文; 王志恒; 张天荣; 赵丽芳

doi:10.3969/j.issn.1006-267x.2016.06.028

动物营养学报 >

2016 , Vol. 28 >Issue 6: 1850 - 1858

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.06.028

研究简报 Short Communications

不同无机磷水平饲粮添加植酸酶对断奶仔猪骨骼钙磷代谢的影响

王德海 ,
杨维仁 ,
郭宝林 ,
张崇玉 ,
郭文文 ,
王志恒 ,
张天荣 ,
赵丽芳

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1. 山东农业大学动物科技学院, 泰安 271081;
2. 北京昕大洋科技发展有限公司, 北京 100081;
3. 临沂新程金锣牧业有限公司, 临沂 276402

收稿日期: 2015-12-16

网络出版日期: 2016-06-27

基金资助

山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-05)

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Effects of Different Inorganic Phosphorus Levels on Bone Calcium and Phosphorus Metabolism of Weaned Piglets Fed Phytase Supplementation Diets

WANG Dehai ,
YANG Weiren ,
GUO Baolin ,
ZHANG Chongyu ,
GUO Wenwen ,
WANG Zhiheng ,
ZHANG Tianrong ,
ZHAO Lifang

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1. College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China;
2. Beijing Smistyle Sci. & Tech. Development Co., Ltd., Beijing 100081, China;
3. Linyi Xincheng Jinluo Animal Husbandry Co., Ltd., Linyi 276402, China

Received date: 2015-12-16

Online published: 2016-06-27

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摘要

本试验旨在研究不同无机磷水平饲粮添加植酸酶对断奶仔猪骨骼钙磷代谢的影响。试验选用192头初重(9.12±0.29) kg、健康状况良好的"杜×长×大"三元杂交断奶仔猪,随机分为6组,每组4个重复,每个重复8头。对照组饲喂基础饲粮(磷为6.00 g/kg);试验组在基础饲粮中添加750 U/kg的植酸酶,无机磷添加量分别为对照组的100%、75%、50%、25%和0(钙磷比为1.17~1.79)。试验期35 d。在加植酸酶的条件下,降低无机磷的添加量,胫骨和跖骨的钙磷及灰分含量以及肋骨磷含量降低且呈线性和二次相关(P<0.05);血清成纤维细胞生长因子(FGF23)含量以及骨合成生化指标骨钙蛋白(BGP)、护骨素(OPG)含量和骨特异性碱性磷酸酶(BAP)活性降低且呈线性和二次相关(P<0.05);血清降钙素(CT)含量,骨吸收生化指标Ⅰ型胶原C端肽(CTX-Ⅰ)、骨桥蛋白(OPN)含量,抗酒石酸酸性磷酸酶(TRACP-5b)以及1,25-二羟维生素D₃[l,25-(OH)₂D₃]活性升高且呈线性和二次相关(P<0.05)。无机磷水平为75%的加植酸酶组与对照组相比,骨钙磷及灰分含量、骨合成生化指标、骨吸收生化指标差异不显著(P>0.05);无机磷水平为50%的加植酸酶组与对照组相比,骨灰分含量、骨合成生化指标差异不显著(P>0.05),骨吸收生化指标显著升高(P<0.05)。与对照组相比,上述2组血清l,25-(OH)₂D₃活性、CT含量显著升高(P<0.05),FGF23含量显著降低(P<0.05)。综上所述,在加植酸酶的条件下,胫骨和跖骨的钙磷和灰分含量以及肋骨磷含量与无机磷的添加量呈线性和二次相关;骨代谢激素及骨代谢生化指标能准确反映骨代谢状况,且比常规指标更敏感。综合各项指标,在断奶仔猪饲粮中添加植酸酶可以替代25%(0.52 g/kg)的无机磷添加量。

关键词： 断奶仔猪; 磷; 植酸酶; 骨骼; 骨代谢生化指标

本文引用格式

王德海 , 杨维仁 , 郭宝林 , 张崇玉 , 郭文文 , 王志恒 , 张天荣 , 赵丽芳 . 不同无机磷水平饲粮添加植酸酶对断奶仔猪骨骼钙磷代谢的影响[J]. 动物营养学报, 2016 , 28(6) : 1850 -1858 . DOI: 10.3969/j.issn.1006-267x.2016.06.028

Abstract

The objective of this study was to estimate the influence of different inorganic phosphorus level diets supplemented with phytase on bone calcium and phosphorus metabolism of weaned piglets. A total of 192 healthy post-weaned piglets (Duroc×Landrace×Largewhite) with an average body weight of (9.12±0.29) kg were randomly divided into 6 groups with 4 replicates in each group, and 8 piglets per replicate. Control group was fed a basal diet (phosphorus content was 6.00 g/kg), experimental groups were fed diets supplemented with 750 U/kg phytase, and inorganic phosphorus addition level was 100%, 75%, 50%, 25% and 0 (calcium-phosphorus ratio was 1.17 to 1.79) of the control group, respectively. The experiment lasted for 35 d. The results showed that with phytase supplementation, the dietary inorganic phosphorus level reduction resulted in a linear and quadratic decrease in contents of tibia and metatarsal bone calcium, phosphorus and ash and the rib bone phosphorus content (P<0.05), and got the same effects on contents of serum fibroblast growth factors (FGF23), biochemical indices of bone synthesis such as bone gla protein (BGP) and osteoprotegerin (OPG) contents, and bone-specific alkaline phosphatase (BAP) activity (P<0.05); it also resulted in a linear and quadratic increase in serum calcitonin (CT) content, biochemical indices of bone resorption such as contents of C-terminal telopeptide of typeⅠcollagen (CTX-Ⅰ) and osteopontin (OPN), and tartrate-resistant acid phosphatase (TRAP5b) activity, as well as 1,25-dihydroxyvitamin D [l,25-(OH)₂D₃] activity (P<0.05). Compared with control group, 75% inorganic phosphorus level + phytase group had no significant difference on contents of bone calcium, phosphorus and ash, and biochemical indices of bone synthesis and resorption (P>0.05); 50% inorganic phosphorus level+phytase group had no significant difference on bone ash content and biochemical indices of bone synthesis (P>0.05), but got significantly higher biochemical indices of bone resorption (P<0.05). Meanwhile, these two groups above both had significantly higher serum l,25-(OH)₂D₃ activity and CT content (P<0.05), but a significantly lower FGF23 content compared with control group (P<0.05). In summary, with phytase supplementation in diets, contents of calcium, phosphorus and ash in tibias and metatarsus and phosphorus in ribs are linear and quadratic correlated with inorganic phosphorus addition level. Hormones and biochemical indices of bone metabolism can accurately reflect the situation of bone metabolism, and are more sensitive than conventional indicators. Dietary phytase supplementation for weaned piglets can replace 25% (0.52 g/kg) inorganic phosphorus.

Key words： weaned piglets; phosphorus; phytase; bone; bone metabolism biochemical indices

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