研究简报 Short Communications

黄芪多糖对种公鸡不同组织miR-16表达的影响及其功能预测分析

  • 任小春 ,
  • 李玉龙 ,
  • 武圣儒 ,
  • 雷新宇 ,
  • 郭伟 ,
  • 党燕娜 ,
  • 龚月生 ,
  • 杨小军
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  • 西北农林科技大学动物科技学院, 杨凌 712100

收稿日期: 2015-12-22

  网络出版日期: 2016-06-27

基金资助

国家自然科学基金项目(31272464);教育部新世纪优秀人才项目(NCET-12-0476);陕西省农业公关计划(2014K01-18-02,2015NY149);陕西省科技统筹创新工程计划项目(2015KTCQ02-19)

Effects and Bioinformatic Analysis of Astragalus Polysaccharide on miR-16 Expression in Different Tissue of Breeder Cocks

  • REN Xiaochun ,
  • LI Yulong ,
  • WU Shengru ,
  • LEI Xinyu ,
  • GUO Wei ,
  • DANG Yanna ,
  • GONG Yuesheng ,
  • YANG Xiaojun
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  • College of Animal Science and Technology, Northwest A & F University, Yanling 712100, China

Received date: 2015-12-22

  Online published: 2016-06-27

摘要

本试验研究黄芪多糖(APS)对种公鸡不同组织中miR-16表达的影响,结合miR-16预测靶基因的GO和KEGG功能富集性分析,旨在探讨APS对不同组织功能的潜在调控作用。选取1日龄科宝500父母代肉种鸡64只,随机分为对照组和APS组,每组4个重复,每个重复8只鸡。对照组饲喂玉米-豆粕型基础饲粮,APS组在基础饲粮中添加10 g/kg APS,试验期40周。结果表明:1)相比于对照组,APS组可以提高种公鸡的采精量和有效精子数,降低精子畸形率,但差异不显著(P>0.05)。2)miR-16具有组织差异性,APS可显著或极显著上调肝脏、脾脏、十二指肠黏膜、回肠黏膜miR-16的表达(P<0.05或P<0.01),显著降低睾丸miR-16的表达(P<0.05)。3)对miR-16预测靶基因进行GO富集分析,表明预测靶基因在跨膜运输、泛素依赖的蛋白质降解、胞内蛋白运输、糖酵解等物质代谢相关生物过程显著或极显著富集(P<0.05或P<0.01)。KEGG富集分析表明,miR-16靶基因在黏着斑、胰岛素信号通路、糖酵解/糖异生等与机体物质代谢、细胞增殖分化相关通路显著富集(P<0.05),在Toll样受体信号通路、自然杀伤细胞介导的细胞毒性等免疫相关通路也有富集。由此可见,APS上调种公鸡脾脏、肝脏、十二指肠黏膜、回肠黏膜组织的miR-16表达,下调睾丸miR-16表达;miR-16预测靶基因与物质代谢、免疫调控密切相关。APS通过调控种公鸡不同组织miR-16表达,影响机体物质代谢和免疫调控功能。

本文引用格式

任小春 , 李玉龙 , 武圣儒 , 雷新宇 , 郭伟 , 党燕娜 , 龚月生 , 杨小军 . 黄芪多糖对种公鸡不同组织miR-16表达的影响及其功能预测分析[J]. 动物营养学报, 2016 , 28(6) : 1887 -1898 . DOI: 10.3969/j.issn.1006-267x.2016.06.032

Abstract

This experiment was conducted to investigate the effects of Astragalus polysaccharides (APS) on miR-16 expression in different tissues of breeder cocks, combining with the functional enrichment analysis of GO and KEGG pathway of miR-16 predicted targets, and to explore the potential regulation role of APS in different tissues via miR-16. A total of 64 one-day-old Cobb 500 breeder cocks were randomly divided into control group and APS group with 4 replicates per group and 8 breeder cocks per replicate. Cocks in the control group were fed a corn-soybean meal based diet, and the others in APS group were fed the basal diet supplemented with 10 g/kg APS. The experiment lasted for 40 weeks. The results showed as follows: 1) compared with the control group, dietary supplemented with APS improved the semen volume and number of effective sperm, decreased the percentage of abnormal sperm (P>0.05). 2) miR-16 differentially expressed in different tissues; APS ssignificantly increased the miR-16 expression in liver, spleen, duodenum mucosa and ileum mucosa (P<0.05 or P<0.01), and down-regulated miR-16 expression in testis (P<0.05). 3) GO enrichment analysis of miR-16 predicted that targets were showed to be enriched for the metabolic functions in transmembrane transport, ubiquitin-dependent protein catabolic process, intracellular protein transport, glycolytic process (P<0.05 or P<0.01). KEGG pathway showed that the target genes were enriched in focal adhesion, insulin signaling pathway, glycolysis/gluconeogenesis associated with cell proliferation, differentiation and metabolism (P<0.05). And the target genes were also enriched in toll-like receptors signaling pathways and natural killer cell mediated cytotoxicity related to immune pathways. In conclusion, APS up-regulates the expression of miR-16 in liver, spleen, duodenum mucosa, ileum mucosa, while down-regulates that in testis of breeder cocks. miR-16 predicted targets collect with metabolism and immune regulation, it showes that APS regulated the miR-16 expression on different tissues of breeder cocks to affect the metabolism and immune regulation functions.

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