研究简报 Short Communications

颈静脉灌注精氨酸对泌乳中期奶牛血清生化和免疫指标的影响

  • 丁洛阳 ,
  • 梁祥焕 ,
  • 王梦芝 ,
  • 张军 ,
  • 王洪荣 ,
  • 周刚 ,
  • 张鑫 ,
  • 徐巧云
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  • 1. 扬州大学动物科学与技术学院, 扬州 225009;
    2. 扬州大学实验农牧场, 扬州 225009

收稿日期: 2015-12-30

  网络出版日期: 2016-06-27

基金资助

江苏省自然科学基金基础研究项目(BK20141270,BK20151312);江苏省高校研究生创新工程项目(KYLX14_1358);江苏省优势学科(PAPD)

Effects of Arginine Infusion through Jugular Vein on Serum Biochemical and Immune Indices of Dairy Cows at Mid-Lactation

  • DING Luoyang ,
  • LIANG Xianghuan ,
  • WANG Mengzhi ,
  • ZHANG Jun ,
  • WANG Hongrong ,
  • ZHOU Gang ,
  • ZHANG Xin ,
  • XU Qiaoyun
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  • 1. College of Animal and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Experimental Farm of Yangzhou University, Yangzhou 225009, China

Received date: 2015-12-30

  Online published: 2016-06-27

摘要

本文旨在研究颈静脉灌注精氨酸对泌乳中期奶牛血清生化和免疫指标的影响。采用3×3复式拉丁方试验设计,选择6头体重、胎次、泌乳期、泌乳量和体况基本一致的荷斯坦奶牛为试验动物,随机分为3组(每组2头),分别为酪蛋白组(对照组)、精氨酸组(灌注37.66 g/d)、丙氨酸组(与精氨酸组等氮,灌注77.24 g/d);每个试验期为22 d(7 d灌注+15 d间隔),在每个试验灌注期的最后1 d晨饲前采血,测定血清生化和免疫指标。结果表明:精氨酸组的奶牛血清中总蛋白、免疫球蛋白G(IgG)、肿瘤坏死因子α(TNF-α)浓度要显著高于其他2组(P<0.05),白球比、白细胞介素1(IL-1)浓度显著高于丙氨酸组(P<0.05),但是牛奶中体细胞数显著低于其他2组(P<0.05),而血清中谷丙转氨酶、谷草转氨酶活性,免疫球蛋白A(IgA)、免疫球蛋白E(IgE)、白细胞介素6(IL-6)浓度在组间无显著差异(P>0.05)。综上,灌注精氨酸能够通过提高血清总蛋白、IgG及IL-1、TNF-α的浓度,从而一定程度上增强泌乳奶牛机体的免疫力和胎儿的被动免疫力。

本文引用格式

丁洛阳 , 梁祥焕 , 王梦芝 , 张军 , 王洪荣 , 周刚 , 张鑫 , 徐巧云 . 颈静脉灌注精氨酸对泌乳中期奶牛血清生化和免疫指标的影响[J]. 动物营养学报, 2016 , 28(6) : 1899 -1906 . DOI: 10.3969/j.issn.1006-267x.2016.06.033

Abstract

This study was conducted to investigate the effects of arginine infusion through jugular vein on serum biochemical and immune indices of dairy cows at mid-lactation. A 3×3 Latin square trail was used. Six healthy lactating cows at similar lactation stages with similar body weight, parities, days in milk, milk yield and body condition were selected and divided into 3 groups (2 for each group), which were casein group (control group), arginine group (infused 37.66 g/d), and alanine group (equal nitrogen to arginine group, infused 77.24 g/d); three periods of test were carried out, and each period lasted for 22 d (7-day infusion period plus 15-day interval period). Blood was collected before morning feeding at the last day of infusion period to detect serum biochemical and immune indices. The results showed as follows: the concentrations of total protein, immunoglobulin G (IgG), tumor necrosis factor α (TNF-α) in serum in arginine group were significantly higher than those in other groups (P<0.05), the concentrations of interleukin 1 (IL-1) and the ratio of albumin to globulin in arginine group were significantly higher than those in alanine group (P<0.05), and the number of somatic cells in milk in arginine group was significantly lower than that in other 2 groups (P<0.05), but no significant differences were found in the activities of gluamic-pyruvic transaminase and glutamic oxalacetic transaminase, and the concentrations of immunoglobulin A (IgA), immunoglobulin E (IgE) and interleukin 6 (IL-6) among groups (P>0.05). In conclusion, arginine infusion can promote immunity performance of lactating cows and the passive immunity of fetus in some degree by increasing serum total protein, IgG, IL-1 and TNF-α concentrations.

参考文献

[1] COOK M E.Triennial growth symposium:a review of science leading to host-targeted antibody strategies for preventing growth depression due to microbial colonization[J].Journal of Animal Science,2011,89(7):1981-1990.  
[2] 段晓燕,柏辛喆,李东清.畜禽营养对免疫应激的影响[J].畜牧兽医杂志,2014,33(2):29-31,41.
[3] LI P,YIN Y L,LI D F,et al.Amino acids and immune function[J].British Journal of Nutrition,2007,98(2):237-252.  
[4] WU G Y,MEININGER C J,KNABE D A,et al.Arginine nutrition in development,health and disease[J].Current Opinion in Clinical Nutrition and Metabolic Care,2000,3(1):59-66.  
[5] 刘凤菊,呙于明,王磊.1-3周龄雌性肉仔鸡精氨酸需要量[J].动物营养学报,2011,23(4):571-577.
[6] ZHOU Q C,ZENG W P,WANG H L,et al.Dietary arginine requirement of juvenile yellow grouper Epinephelus awoara[J].Aquaculture,2012,350-353:175-182.
[7] 曾雯娉.凡纳滨对虾幼虾对赖氨酸、蛋氨酸、精氨酸和苯丙氨酸需要量的研究[D].硕士学位论文.湛江:广东海洋大学,2012:24-30.
[8] KETTELER M,IKEGAYA N,BREES D K,et al.L-arginine metabolism in immune-mediated glomerulonephritis in the rat[J].American Journal of Kidney Diseases,1996,28(6):878-887.  
[9] JAHANIAN R.Immunological responses as affected by dietary protein and arginine concentrations in starting broiler chicks[J].Poultry Science,2009,88(9):1818-1824.  
[10] ZHAO H Y,ZHAO H Y,WANG Y,et al.Retracted article:randomized clinical trial of arginine-supplemented enteral nutrition versus standard enteral nutrition in patients undergoing gastric cancer surgery[J].Journal of Cancer Research and Clinical Oncology,2013,139(9):1465-1470.  
[11] 曾静.L-精氨酸对热应激小鼠免疫功能的影响及其可能机制[D].硕士学位论文.广州:中国人民解放军第一军医大学,2003:33-40.
[12] 张灿菲.精氨酸对急性热应激鸡肠道黏膜免疫的影响[D].硕士学位论文.武汉:华中农业大学,2008:22-29.
[13] ZHU W,JIANG W,WU L Y.Dietary L-arginine supplement alleviates hepatic heat stress and improves feed conversion ratio of Pekin ducks exposed to high environmental temperature[J].Journal of Animal Physiology and Animal Nutrition,2014,98(6):1124-1131.  
[14] WANG M Z,XU B L,WANG H R,et al.Effects of arginine concentration on the in vitro expression of casein and mTOR pathway related genes in mammary epithelial cells from dairy cattle[J].PLoS One,2014,9(5):e95985.
[15] SODA K,DOBASHI Y,KANO Y,et al.Polyamine-rich food decrease age-associated pathology and mortality in aged mice[J].Experimental Gerontology,2009,44(11):727-732.  
[16] FANG Y Z,YANG S,WU G Y.Free radicals,antioxidants,and nutrition[J].Nutrition,2002,18(10):872-879.  
[17] BOGDAN C.Nitric oxide and the immune response[J].Nature Immunology,2001,2(10):907-916.  
[18] EFRON D,BARBUL A.Role of arginine in immunonutrition[J].Journal of Gastroenterology,2000,35(Suppl.12):20-23.
[19] WIESINGER H.Arginine metabolism and the synthesis of nitric oxide in the nervous system[J].Progress in Neurobiology,2001,64(4):365-391.  
[20] LAMAS B,VERGNAUD-GAUDUCHON J,GONCALVES-MENDES N,et al.Altered functions of natural killer cells in response to L-arginine availability[J].Cellular Immunology,2012,280(2):182-190.  
[21] 廖明,辛朝安,张春红,等.家禽免疫学基础知识[J].中国兽医杂志,1996,22(1):49-51.
[22] 张和平,郭军.乳中特异性免疫球蛋白(IgG乳抗体)的开发及应用[J].乳业科学与技术,2004(3):106-110.
[23] 杨晓宇.莎能奶山羊初乳理化性质及其免疫球蛋白(IgG)的研究[D].博士学位论文.西安:陕西师范大学,2006:8-10.
[24] SVANBORG C.Bacterial adherence and mucosal immunity[M]//OGRA P L,LAMM M E,MCGHEE J R,et al.Handbook of mucosal immunology.New York:Academic Press,1994:71-78.
[25] CHEN D W,FEI Z W,ZHANG Y C,et al.Role of enteral immunonutrition in patients with gastric carcinoma undergoing major surgery[J].Asian Journal of Surgery,2005,28(2):121-124.  
[26] SHANG H F,WANG Y Y,LAI Y N,et al.Effects of arginine supplementation on mucosal immunity in rats with septic peritonitis[J].Clinical Nutrition,2004,23(4):561-569.  
[27] PEREZ-CARBAJAL C,CALDWELL D,FARNELL M,et al.Immune response of broiler chickens fed different levels of arginine and vitamin E to a coccidiosis vaccine and Eimeria challenge[J].Poultry Science,2010,89(9):1870-1877.  
[28] TAN J Z,APPLEGATE T J,LIU S S,et al.Supplemental dietary L-arginine attenuates intestinal mucosal disruption during a coccidial vaccine challenge in broiler chickens[J].British Journal of Nutrition,2014,112(7):1098-1109.  
[29] 杨慧,林登峰,林伯全,等.饲粮添加不同水平L-精氨酸对泌乳母猪生产性能、血清氨基酸浓度和免疫生化指标的影响[J].动物营养学报,2012,24(11):2103-2109.
[30] 杨爱君,阮征,曾庆孝.控制体细胞数在优质生鲜奶生产中的应用[J].中国乳品工业,2005,33(1):53-56.
[31] 唐平.牛奶体细胞快速检测方法的研究[D].硕士学位论文.杭州:浙江大学,2006:2-4.
[32] 刘星达,彭瑛,吴信,等.精氨酸和精氨酸生素对母猪泌乳性能及哺乳仔猪生长性能的影响[J].饲料工业,2011,32(8):14-15.
[33] 李新国.精氨酸在早期断奶仔猪营养中的应用研究[D].硕士学位论文.长沙:湖南农业大学,2010:34-42.
[34] 廖英杰,刘波,任鸣春,等.精氨酸对团头鲂幼鱼生长、血清游离精氨酸和赖氨酸、血液生化及免疫指标的影响[J].中国水产科学,2014,21(3):549-559.
[35] ZHOU Q C,ZENG W P,WANG H L,et al.Dietary arginine requirement of juvenile Pacific white shrimp,Litopenaeus vannamei[J].Aquaculture,2012,364-365:252-258.
[36] 刘玉兰,黄晶晶,范伟,等.L-精氨酸对脂多糖刺激断奶仔猪生产性能、血液生化指标和内脏器官重量的影响[J].动物营养学报,2008,20(2):140-145.
[37] FRANK J W,ESCOBAR J,NGUYEN H V,et al.Oral N-carbamylglutamate supplementation increases protein synthesis in skeletal muscle of piglets[J].The Journal of Nutrition,2007,137(2):315-319.
[38] 杜强.卵形鲳鲹赖氨酸和蛋氨酸需求量及饲料中鱼粉替代的研究[D].硕士学位论文.上海:上海海洋大学,2012.
[39] 潘杰,陈荣军,姚康,等.精氨酸对断奶仔猪生长性能·器官重及血液生化参数的影响[J].安徽农业科学,2009,37(7):2981-2982,2990.
[40] NIJSTEN MW,HACK CE,HELLE M,et al.Interleukin-6 and its relation to the humoral immune response and clinical parameters in burned patients[J].Surgery,1991,109(6):761-767.
[41] AKIRA S,HIRANO T,TAGA T,et al.Biology of multifunctional cytokines:IL 6 and related molecules (IL 1 and TNF)[J].The FASEB Journal,1990,4(11):2860-2867.
[42] KOSS M,PFEIFFER Ⅱ G R,WANG Y,et al.Ezrin/radixin/moesin proteins are phosphorylated by TNF-α and modulate permeability increases in human pulmonary microvascular endothelial cells[J].The Journal of Immunology,2006,176(2):1218-1227.  
[43] ZHANG W D,CHEN Z,LI F,et al.Tumour necrosis factor-α (TNF-α) transgene-expressing dendritic cells (DCs) undergo augmented cellular maturation and induce more robust T-cell activation and anti-tumour immunity than DCs generated in recombinant TNF-α[J].Immunology,2003,108(2):177-188.  
[44] 郭祎玮.精氨酸对肉仔鸡生长性能和免疫功能的影响及其机理研究[D].硕士学位论文.呼和浩特:内蒙古农业大学,2014:31-36.
[45] TAN J Z,LIU S S,GUO Y M,et al.Dietary L-arginine supplementation attenuates lipopolysaccharide-induced inflammatory response in broiler chickens[J].British Journal of Nutrition,2014,111(8):1394-1404.  
[46] LIU Y L,HUANG J J,HOU Y Q,et al.Dietary arginine supplementation alleviates intestinal mucosal disruption induced by Escherichia coli lipopolysaccharide in weaned pigs[J].British Journal of Nutrition,2008,100(3):552-560.  
[47] CHATTERJEE S,PREMACHANDRAN S,BAGEWADIKAR R S,et al.Arginine metabolic pathways determine its therapeutic benefit in experimental heatstroke:role of Th1/Th2 cytokine balance[J].Nitric Oxide,2006,15(4):408-416.  
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