研究简报 Short Communications

含钒蛋黄粉对大鼠生长、氧化应激状态及其相关基因表达的影响

  • 崔仁勇 ,
  • 王建萍 ,
  • 张克英 ,
  • 丁雪梅 ,
  • 曾秋凤 ,
  • 白世平 ,
  • 罗玉衡
展开
  • 四川农业大学动物营养所, 教育部抗病营养重点实验室, 雅安 625014

收稿日期: 2016-01-07

  网络出版日期: 2016-06-27

基金资助

四川省科技支撑项目(2011NZ0073,2014NZ0002);科技部科技支撑项目(2014BAD13B04);四川省教育厅项目(13ZB0290)

Effects of Egg Yolk Powder Including Vanadium on Growth, Oxidative Stress Status and Its Related Gene Expression of Wistar Rats

  • CUI Renyong ,
  • WANG Jianping ,
  • ZHANG Keying ,
  • DING Xuemei ,
  • ZENG Qiufeng ,
  • BAI Shiping ,
  • LUO Yuheng
Expand
  • Key Laboratory of Animal Disease-Resistance of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2016-01-07

  Online published: 2016-06-27

摘要

钒是一种重金属,过量的摄入会造成蛋鸡氧化应激,降低鸡蛋品质并残留到鸡蛋中,影响鸡蛋的安全。本研究主要通过给大鼠饲喂含钒的蛋黄粉,考察其对大鼠生长性能、氧化应激状态及其相关基因表达的影响,为评价钒(有机钒)的生物安全性提供依据。选用27只4周龄雌性Wistar大鼠单笼饲养,设3个处理,每个处理9只,分别在饲粮中添加600 g/kg采食3种钒水平(0、5和10 mg/kg)饲粮的蛋鸡所产鸡蛋制备的蛋黄粉。经实测,3种饲粮分别含钒0.107、0.137和0.164 mg/kg。试验期35 d。结果表明:3个处理大鼠生长性能、器官指数,血浆甘油三酯、丙二醛(MDA)、尿素氮含量及谷丙转氨酶(ALT)、谷草转氨酶(AST)活性,肝脏、肾脏超氧化物歧化酶活性、MDA含量、总抗氧化能力,肝脏ALT、AST活性、谷胱甘肽含量和谷胱甘肽巯基转移酶活性,以及肝脏、肾脏组织结构、肾脏钒残留量均无显著差异(P>0.05)。与处理1相比,处理2、3大鼠肝脏醌氧化还原酶1(NQO1)活性显著下降(P<0.05),NQO1和核因子E2相关因子2 mRNA相对表达量也显著下降(P<0.05)。结果提示:大鼠饲粮中添加600 mg/kg不同钒含量的蛋黄粉对大鼠生长性能和机体氧化还原状态的影响无显著差异,但含钒0.137、0.164 mg/kg可以降低NQO1酶活性并下调抗氧化应答相关基因的表达。

本文引用格式

崔仁勇 , 王建萍 , 张克英 , 丁雪梅 , 曾秋凤 , 白世平 , 罗玉衡 . 含钒蛋黄粉对大鼠生长、氧化应激状态及其相关基因表达的影响[J]. 动物营养学报, 2016 , 28(6) : 1956 -1964 . DOI: 10.3969/j.issn.1006-267x.2016.06.039

Abstract

As a kind of heavy metal, excessive intake of vanadium can cause oxidative stress to laying hens, reduce egg quality and make residue in eggs, which affects the safety of eggs. The objective of this study was to explore the effects of feeding egg yolk powder including vanadium to Wistar rats on their growth, oxidative stress status and its related gene expression, and to evaluate the biosecurity of organic vanadium. A total of 27 female Wistar rats (4 weeks old) were allocated to 3 treatments with 9 rats each, and fed three kinds of diets supplemented with 600 g/kg egg yolk powders which were prepared with eggs from laying hens fed diets containing 0, 5 and 10 mg/kg vanadium, respectively. The three diets actually contained 0.107, 0.137 and 0.164 mg/kg vanadium. The experiment lasted for 35 d. The results showed that there were no significant differences on growth performance, organ indices, contents of plasma triglyceride, malonaldehyde (MDA) and urea nitrogen, activities of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST), liver and kidney superoxide dismutase (SOD) activity, MDA content, and total antioxidant capacity (T-AOC), activities of liver ALT and AST, liver glutathione content, and liver glutathione S-transferase activity, as well as histomorphology of liver and kidney and vanadium residual in kidney of rats among 3 treatments (P>0.05). However, compared with treatment 1, liver quinone oxidoreductase 1 (NQO1) activity of rats in treatments 2 and 3 was significantly decreased (P<0.05), and the relative expression levels of liver NQO1 and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA were significantly decreased (P<0.05), too. The results indicate that the dietary supplementation of 600 mg/kg egg yolk powders with different vanadium content has no significantly different effects on growth performance and oxidation-reduction state of Wistar rats. However, the NQO1 activity, mRNA expression of NQO1 and Nrf2 are down-regulated as the vanadium content increasing (0.137 and 0.164 mg/kg).

参考文献

[1] SCHWARZ K,MILNE D B.Growth effects of vanadium in the rat[J].Science,1971,174(4007):426-428.  
[2] HAMEL F G,DUCKWORTH W C.The relationship between insulin and vanadium metabolism in insulin target tissues[J].Molecular and Cellular Biochemistry,1995,153(1/2):95-102.
[3] ADACHI A,ASAI K,KOYAMA Y,et al.Subacute toxicity of vanadium in rats[J].Japanese Journal of Toxicology and Environmental Health,1997,43(1):27.
[4] LIU J,CUI H M,LIU X D,et al.Dietary high vanadium causes oxidative damage-induced renal and hepatic toxicity in broilers[J].Biological Trace Element Research,2012,145(2):189-200.  
[5] DOMINGO J L,GOMEZ M,SANCHEZ D J,et al.Toxicology of vanadium compounds in diabetic rats:the action of chelating agents on vanadium accumulation[J].Molecular and Cellular Biochemistry,1995,153(1):233-240.
[6] UEBERSCHÄR K H,VOGT H,MATTHES S.Der einfluss von vanadium-zäsatzen zum broiler-und legehennenfutter auf die leistungender der tiere und auf rückstandsgehalte in den geweben und eiern[J].Archiv fur Geflugelkunde,1985,49:4-8.
[7] IMURA H,SHIMADA A,NAOTA M,et al.Vanadium toxicity in mice:possible impairment of lipid metabolism and mucosal epithelial cell necrosis in the small intestine[J].Toxicologic Pathology,2013,41(6):842-856.  
[8] REEVES P G,NIELSEN F H,FAHEY G C,Jr.,et al.AIN-93 purified diets for laboratory rodents:final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet[J].The Journal of Nutrition,1993,123(11):1939-1951.
[9] 梁立娜,李中阳,陈登云,等.ICP-MS技术测定生物样品中重金属的前处理方法研究[J].环境化学,2003,22(1):99-100.
[10] 梁彦秋,潘伟,铁梅,等.ICP-MS测定动物肝脏中铅、镉和砷[J].光谱实验室,2006,23(2):234-237.
[11] HABEOS I G,ZIROS P G,CHARTOUMPEKIS D,et al.Simvastatin activates Keap1/Nrf2 signaling in rat liver[J].Journal of Molecular Medicine,2008,86(11):1279-1285.  
[12] YURYEV A.PCR primer design[M].Totowa,New Jersey:Humama Press,2007.
[13] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J].Methods,2001,25(4):402-408.  
[14] 魏磊磊.钒营养研究进展[J].中国饲料,2004(9):8-10.
[15] DANIEL E P,LILLIE R D.Experimental vanadium poisoning in the white rat[J].Public Health Reports,1938,53(19):765-777.  
[16] LUCKEY T D,VENUGOPAL B,HUTCHESON D.Heavy metal toxicity,safety,and hormonology[M].New York:Academic Press,1975.
[17] ANKE M.Vanadium:an element both essential and toxic to plants,animals and humans[J].Anales de la Real Academia Farmacia,2005,70(4):961-999.
[18] FAULKNER T G.Vanadium:toxicology and biological significance[M].London:Elsevier,1964.
[19] DOMINGO J L,LLOBET J M,TOMAS J M,et al.Short-term toxicity studies of vanadium in rats[J].Journal of Applied Toxicology,1985,5(6):418-421.  
[20] 孙素玲,周源,刘森,等.偏钒酸铵对小鼠血清和肝脏转氨酶活力的影响[J].生态毒理学报,2011,6(4):445-448.
[21] CANO-GUTIÉRREZ G,ACEVEDO-NAVA S,SANTAMARÍA A,et al.Hepatic megalocytosis due to vanadium inhalation:participation of oxidative stress[J].Toxicology and Industrial Health,2012,28(4):353-360.  
[22] KENNEDY G C,MITRA J.Body weight and food intake as initiating factors for puberty in the rat[J].The Journal of Physiology,1963,166(2):408-418.  
[23] ADACHI A,ASAI K,KOYAMA Y,et al.Subacute vanadium toxicity in rats[J].Journal of Health Science,2000,46(6):503-508.  
文章导航

/