动物营养学报 >

2016 , Vol. 28 >Issue 7: 2292 - 2299

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.07.037

研究简报 Short Communications

饲料锌含量对方格星虫稚虫生长性能、体成分、体腔液中锌含量及碱性磷酸酶活性的影响

  • 许明珠 ,
  • 张琴 ,
  • 童潼 ,
  • 董兰芳 ,
  • 杨家林 ,
  • 蒋艳 ,
  • 黄国强
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  • 广西壮族自治区海洋研究所, 广西海洋生物技术重点实验室, 北海 536000

收稿日期: 2016-01-12

  网络出版日期: 2016-07-28

基金资助

国家自然科学基金项目（31402304）；广西科学研究与技术开发计划（桂科转13129024，桂科合1346011-12，桂科合14125008-2-20）；广西自然科学基金项目（2015GXNSFBB139005）；广西科学院基本科研业务费（15YJ22HYS15）

收起

Effects of Dietary Zinc Content on Growth Performance, Body Composition, Coelomic Fluid Zinc Content and Alkaline Phosphatase Activity of Juvenile Peanut Worm, Sipunculus nudus Linnaeus

  • XU Mingzhu ,
  • ZHANG Qin ,
  • TONG Tong ,
  • DONG Lanfang ,
  • YANG Jialin ,
  • JIANG Yan ,
  • HUANG Guoqiang
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  • Guangxi Institute of Oceanology, Key Laboratory of Marine Biotechnology of Guangxi, Beihai 536000, China

Received date: 2016-01-12

  Online published: 2016-07-28

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摘要

以蛋氨酸锌为锌源，在等氮等能的基础饲料中添加不同水平的锌，配制实际锌含量分别为9.3、31.7、49.9、90.1、168.6和326.5 mg/kg的6种试验饲料，饲喂平均体重为（14.54±0.10） mg的方格星虫稚虫8周，用以研究饲料锌含量对方格星虫稚虫生长、体成分、体腔液中锌含量及碱性磷酸酶活性的影响。每种试验饲料设3个重复，每个重复饲喂400尾方格星虫稚虫。结果表明：饲料锌含量对方格星虫的成活率、增重率和特定生长率均有显著影响（P<0.05），其中增重率和特定生长率均在饲料锌含量为49.9 mg/kg时达到最高值，并显著高于饲料锌含量最低（9.3 mg/kg）和最高（326.5 mg/kg）的组（P<0.05）。饲料锌含量对虫体粗脂肪和粗灰分含量有显著影响（P<0.05），虫体粗脂肪含量随着饲料锌含量的提高呈先增后降的趋势，而粗灰分含量的趋势则相反。饲料锌含量对虫体水分和粗蛋白质含量没有显著影响（P>0.05）。饲料锌含量对虫体体腔液锌含量和碱性磷酸酶活性均有显著影响（P<0.05）。虫体体腔液锌含量随着饲料锌含量的提高而不断上升，在饲料锌含量为326.5 mg/kg时达到最高值；而虫体体腔液中碱性磷酸酶活性则随着饲料锌含量的提高先上升后趋于稳定，当饲料锌含量为49.9 mg/kg时达到最高值。以增重率为评价指标，通过回归分析得出方格星虫稚虫饲料中适宜锌含量为41.93 mg/kg。

关键词： 方格星虫稚虫; 锌; 生长性能; 体成分; 组织锌含量; 碱性磷酸酶活性

本文引用格式

许明珠 , 张琴 , 童潼 , 董兰芳 , 杨家林 , 蒋艳 , 黄国强 . 饲料锌含量对方格星虫稚虫生长性能、体成分、体腔液中锌含量及碱性磷酸酶活性的影响[J]. 动物营养学报, 2016 , 28(7) : 2292 -2299 . DOI: 10.3969/j.issn.1006-267x.2016.07.037

Abstract

This trial was conducted to investigate the effects of dietary zinc content on growth performance, body composition, coelomic fluid zinc content and alkaline phosphatase (AKP) activity of juvenile peanut worm, Sipunculus nudus Linnaeus (S. nudus). Juvenile peanut worm with the average body weight of (14.54±0.10) mg were fed six isonitrogenous and isoenergetic microparticle diets with different supplemental levels of zinc (zinc methionine as zinc source) for 8 weeks, and the measured zinc contents in diets were 9.3, 31.7, 49.9, 90.1, 168.6 and 326.5 mg/kg, respectively. Each diet had three replicates and each replicate had 400 juvenile peanut worm. The results showed that the dietary zinc content had significant effects on weight gain rate (WGR), specific growth rate (SGR) and survival ratio (SR) of S. nudus (P<0.05). When the dietary zinc content was 49.9 mg/kg, WGR and SGR produced the maximum value, and significantly higher than those in the lowest (9.3 mg/kg) and the highest (326.5 mg/kg) zinc content groups (P<0.05). Dietary zinc content had significant effects on body ether extract and ash contents (P<0.05). With the increase of dietary zinc content, body ether extract content was firstly increased and then decreased, while body ash content showed the opposite trend. No significant differences were observed in body moisture and crude protein contents of S. nudus among all groups (P>0.05). Dietary zinc content significantly affected coelomic fluid zinc content and AKP activity of S. nudus (P<0.05). With the increase of dietary zinc content, the coelomic fluid zinc content of S. nudus was sustained rise, when the dietary zinc content was 326.5 mg/kg, it produced the maximum value. Moreover, the coelomic fluid AKP activity was firstly increased and then tended to be stable, when the dietary zinc content was 49.9 mg/kg, it produced the maximum value. With WGR as the evaluating index, the suitable dietary zinc content of juvenile peanut worm is 41.93 mg/kg by regression analysis.

Key words： juvenile peanut worm; zinc; growth performance; body composition; coelomic fluid zinc content; AKP activity

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