动物营养学报 >

2016 , Vol. 28 >Issue 7: 2300 - 2308

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.07.038

研究简报 Short Communications

不同生长时期虎斑乌贼内壳营养成分含量分析比较

  • 江茂旺 ,
  • 蒋霞敏 ,
  • 梁晶晶 ,
  • 王鹏帅 ,
  • 阮鹏 ,
  • 韩庆喜
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  • 宁波大学海洋学院, 宁波 315211

收稿日期: 2016-01-18

  网络出版日期: 2016-07-28

基金资助

国家自然科学基金项目（41206114）；宁波市农业科技专项（2014C11001）

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Comparative Analysis: Nutritional Component Contents in Cuttlebone of Sepia pharaonis at Different Growth Stages

  • JIANG Maowang ,
  • JIANG Xiamin ,
  • LIANG Jingjing ,
  • WANG Pengshuai ,
  • RUAN Peng ,
  • HAN Qingxi
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  • School of Marine Sciences, Ningbo University, Ningbo 315211, China

Received date: 2016-01-18

  Online published: 2016-07-28

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摘要

通过探究不同生长时期虎斑乌贼内壳的营养成分及矿物元素含量变化，为其开发利用提供理论依据。采用国家标准方法对不同生长时期（60、90、120、150日龄）虎斑乌贼内壳营养成分（水分、粗灰分、粗蛋白质、粗脂肪、多糖、氨基酸、脂肪酸）及矿物元素[5种常量元素——钠（Na）、钾（K）、镁（Mg）、铝（Al）、钙（Ca）和8种微量元素——铜（Cu）、铁（Fe）、锌（Zn）、硒（Se）、锰（Mn）、镉（Cd）、砷（As）、锶（Sr）]的含量进行测定。结果表明：不同生长时期时，粗灰分均是虎斑乌贼内壳的主要成分，含量为89.98%～92.06%，其含量随着生长不断增加，表现为150日龄时显著高于其他日龄时（P<0.05）；粗蛋白质的含量为2.82%～3.11%，其含量随着生长不断增加，表现为150日龄时显著高于60日龄时（P<0.05）；水分含量为3.93%～6.21%，其含量随着生长不断降低，表现为60日龄时显著高于其他日龄时（P<0.05）；相对于其他营养成分，虎斑乌贼内壳中粗脂肪、多糖含量较少，分别为0.41%～0.47%、0.34%～0.38%。不同生长时期的虎斑乌贼内壳中均检测出17种氨基酸，其中7种必需氨基酸（EAA）。不同生长时期的虎斑乌贼内壳中总氨基酸（TAA）、EAA和酸性氨基酸（AAA）含量分别为2.79%～2.93%、0.87%～0.98%和0.69%～0.87%，其中AAA含量随着生长不断增加，表现为150日龄时显著高于其他日龄时（P<0.05）。各生长时期虎斑乌贼内壳中均以谷氨酸（Glu）含量（0.31%～0.43%）最高，组氨酸（His）含量（0.03%～0.07%）最低。不同生长时期的虎斑乌贼内壳中均检测出7种脂肪酸，包括3种饱和脂肪酸（SFA）、1种单不饱和脂肪酸（MUFA）和3种多不饱和脂肪酸（PUFA）；在脂肪酸组分中以C22： 6n-3、C16： 0和C18： 0为主，其中C22： 6n-3含量（35.68%～37.62%）最高。生长时期对常量元素Na、Mg、Al和Ca及微量元素Cu、Zn和Sr含量有显著影响（P<0.05），尤其是Ca、Mg、Zn含量，随着生长不断增加；重金属元素Cd、As含量很低，分别为0.001～0.003 mg/g和0.012～0.030 mg/g。综上所述，虎斑乌贼内壳营养丰富，Ca、Mg、Zn含量高，且随着生长不断增加，具有良好的开发应用前景。

关键词： 虎斑乌贼; 内壳; 不同生长时期; 营养成分; 矿物元素

本文引用格式

江茂旺 , 蒋霞敏 , 梁晶晶 , 王鹏帅 , 阮鹏 , 韩庆喜 . 不同生长时期虎斑乌贼内壳营养成分含量分析比较[J]. 动物营养学报, 2016 , 28(7) : 2300 -2308 . DOI: 10.3969/j.issn.1006-267x.2016.07.038

Abstract

This study was conducted to study the changes of nutritional component contents in cuttlebone of Sepia pharaonis at different growth stages, in order to provide a theoretical basis for development and utilization of Sepia pharaonis. The contents of nutritional components (including moisture, ash, crude protein, crude fat, polysaccharide, amino acids, fatty acids) and minerals [including 5 kinds of major elements-natrium (Na), kalium (K), magnesium (Mg), aluminium (Al), calcium (Ca), and 8 kinds of trace elements-copper (Cu), iron (Fe), zinc (Zn), selenium (Se), manganese (Mn), chromium (Cd), arsenic (As), strontium (Sr)] in cuttlebone of Sepia pharaonis at different growth stages (growing ages were 60, 90, 120 and 150 d, respectively) were determined using national standard nutritional methods. The results showed as follows: among different growth stages, the ash was the main component in cuttlebone of Sepia pharaonis and the content of ash was 89.98% to 92.06%, which was increased with the growth and 150 days of age was significantly higher than other days of age (P<0.05); crude protein content was 2.82% to 3.11%, which was increased with the growth and 150 days of age was significantly higher than 60 days of age (P<0.05); the content of moisture was 3.93% to 6.21%, which was decreased with the growth and 60 days of age was significantly higher than other days of age (P<0.05); the contents of crude fat and polysaccharide were less compared with other components, which were 0.41% to 0.47% and 0.34% to 0.38%, respectively. A total of 17 amino acids were identified in cuttlebone of Sepia pharaonis among different growth stages, including 7 kinds of essential amino acids (EAA). The contents of total amino acid (TAA), EAA and acidic amino acids (AAA) were 2.79% to 2.93%, 0.87% to 0.98% and 0.56% to 0.78%, respectively; the content of AAA was increased with the growth, and 150 days of age was significantly higher than other days of age (P<0.05). The content of glutamate (Glu) was the highest (0.31% to 0.43%) and the content of histidine (His) was the lowest (0.03% to 0.07%) in cuttlebone of Sepia pharaonis among different growth stages. A total of 7 fatty acids were identified, including 3 saturated fatty acids (SFA), 1 monounsaturated fatty acids (MUFA) and 3 polyunsaturated fatty acids (PUFA). C22: 6n-3, C16: 0 and C18: 0 were the main components of fatty acids, among which C22: 6n-3 had the highest value (35.68% to 37.62%). The contents of major elements-Na, Mg, Al and Ca, and trace elements-Cu, Zn and Sr were significantly affected by the growth stage (P<0.05), especially the contents of Ca, Mg and Zn, which were increased with the growth; the contents of heavy metal elements-Cd and As were very low, which were 0.001 to 0.003 mg/g and 0.012 to 0.030 mg/g, respectively. It is concluded that the cuttlebone of Sepia pharaonis has a high nutritional value, Ca, Mg and Zn contents are rich and increase with the growth. Therefore, it has a good spectacle of opening up and application.

Key words： Sepia pharaonis; cuttlebone; different growth stages; nutritional components; mineral elements

参考文献

[1] 高学敏.中药学[M].北京:中国中医药出版社,2002:582.
[2] 陶凯,毛天球,陈富林,等.一种新型羟基磷灰石材料的制备及其细胞相容性探讨[J].生物医学工程学杂志,2006,23(4):887-890.
[3] 周蔚,吴海涛,李采.海螵蛸作为组织工程支架材料的实验研究[J].复旦学报:医学版,2007,34(3):438-441.
[4] 刘源.海螵蛸/骨形态发生蛋白复合人工骨成骨及再血管化的初步研究[D].硕士学位论文.大连:大连医科大学,2012.
[5] AKSAKAL B,DEMIREL M.Synthesis and fabrication of novel cuttlefish (Sepia officinalis) backbone biografts for biomedical applications [J].Ceramics International,2015,41(3):4531-4537.  
[6] CHUNG M T,WANG C H.Age validation of the growth lamellae in the cuttlebone from cultured Sepia pharaonis at different stages[J].Journal of Experimental Marine Biology and Ecology,2013,447:132-137.
[7] 唐丽娟,刘玮炜,王丽,等.不同方法提取海螵蛸多糖及结构表征[J].中成药,2011,33(9):1625-1628.
[8] 唐丽娟,刘玮炜,史大华,等.海螵蛸提取物对脲酶活性的抑制作用初探[J].中华中医药杂志,2012,27(2):331-333.
[9] RAMASAMY P,SUBHAPRADHA N,SHANMUGAM V,et al.Extraction,characterization and antioxidant property of chitosan from cuttlebone Sepia kobiensis (Hoyle 1885) [J].International Journal of Biological Macromolecules,2014,64:202-212.
[10] 顾青青,安叡,张艺竹,等.不同产地海螵蛸中核苷类成分测定[J].中成药,2015,37(5):1016-1021.
[11] SHUSHIZADEH M R,POUR E M,ZARE A,et al.Persian gulf β-chitin extraction from Sepia pharaonis sp.Cuttlebone and preparation of its derivatives[J].Bioactive Carbohydrates and Dietary Fibre,2015,6(2):133-142.  
[12] LEE Y H,CHANG Y C,YAN H Y,et al.Early visual experience of background contrast affects the expression of NMDA-like glutamate receptors in the optic lobe of cuttlefish,Sepia pharaonis[J].Journal of Experimental Marine Biology and Ecology,2013,447:86-92.
[13] HOQUE M S,BENJAKUL S,PRODPRAN T.Effects of hydrogen peroxide and Fenton's reagent on the properties of film from cuttlefish (Sepia pharaonis) skin gelatin[J].Food Chemistry,2011,128(4):878-888.  
[14] 陈道海,郑亚龙.虎斑乌贼(Sepia pharaonis)繁殖行为谱分析[J].海洋与湖沼,2013,44(4):931-936.
[15] 陈道海,王雁,梁汉青,等.虎斑乌贼(Sepia pharaonis)胚胎发育及孵化历期观察[J].海洋与湖沼,2012,43(2):394-400.
[16] 黄建盛,陈刚,张健东,等.野生虎斑乌贼(Sepia pharaonis)肌肉主要营养成分分析及评价[J].营养学报,2014,36(5):502-504.
[17] 高晓兰,蒋霞敏,乐可鑫,等.野生虎斑乌贼不同组织营养成分分析及评价[J].动物营养学报,2014,26(12):3858-3867.
[18] 蒋霞敏,彭瑞冰,唐锋,等.虎斑乌贼规模化人工繁育技术研究[C]//2013年全国海水养殖学术研讨会论文集.绍兴:中国水产学会,浙江省水产学会,2013:46-47.
[19] 赵云涛,李琨,黄燕,等.海螵蛸入药部分和废弃部分8种无机元素分析[J].微量元素与健康研究,2003,20(2):35-37.
[20] 杨振萍,边清泉.火焰原子吸收光谱法测定海螵蛸中15种微量元素含量[J].食品科学,2010,31(6):190-192.
[21] 李兰,吴启南.海螵蛸的化学成分研究[J].现代中药研究与实践,2009,23(2):52-54.
[22] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.3-2003 食品中水分的测定[S].北京:中国标准出版社,2004:25-29.
[23] 中华人民共和国卫生部.GB 5009.4-2010食品中灰分的测定[S].北京:中国标准出版社,2010:1-2.
[24] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.5-2003食品中蛋白质的测定[S].北京:中国标准出版社,2004:37-41.
[25] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.6-2003食品中脂肪的测定[S].北京:中国标准出版社,2004:45-46.
[26] 郭金龙,陈有君,孙国琴,等.苯酚-硫酸法测定杏鲍菇多糖方法的研究[J].食品科学,2008,29(12):555-558.
[27] 孙磊,乔善义,赵毅民.黑骨藤多糖含量测定方法研究[J].中国中药杂志,2009,34(10):1241-1244.
[28] 张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社,1999:11-12.
[29] 中华人民共和国卫生部,中国国家标准化管理委员会.GB/T 5009.124-2003食品中氨基酸的测定[S].北京:中国标准出版社,2004:115-119.
[30] 姚林杰,叶元土,蔡春芳,等.饲料蛋白质脂肪比与不同生长阶段团头鲂全鱼蛋白质、脂肪含量及肌肉氨基酸、脂肪酸组成的关系[J].动物营养学报,2014,26(8):2184-2196.
[31] DAUPHIN Y.The organic matrix of coleoid cephalopod shells:molecular weights and isoelectric properties of the soluble matrix in relation to biomineralization processes[J].Marine Biology,1996,125(3):525-529.
[32] 肖述.乌贼海螵蛸形成机理研究[D].硕士学位论文.青岛:中国海洋大学,2003.
[33] 赵中杰,江佩芬,李昂.海螵蛸中碳酸钙、微量元素和氨基酸的测定[J].中国中药杂志,1990,15(1):41-43.
[34] KEITH J,STOCKWELL S,BALL D,et al.Comparative analysis of macromolecules in mollusc shells[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1993,105(3/4):487-496.
[35] WEINERS S,HOOD L.Soluble protein of the organic matrix of mollusk shells:a potential template for shell formation[J].Science,1975,190(4218):987-989.  
[36] KAPLAN D L.Mollusc shell structures:novel design strategies for synthetic materials[J].Current Opinion in Solid State and Materials Science,1998,3(3):232-236.  
[37] MANN S.Molecular recognition in biomineralization[J].Nature,1988,332(10):119-124.
[38] 刘建军,陈卫红.中华猕猴桃微量元素测定分析[J].广东微量元素科学,2001,8(4):64-65.
[39] 夏静芬,钱国英,陈亮,等.珍珠粉和贝壳粉的化学成分和结构特征分析[J].化学研究与应用,2010,22(11):1467-1471.
[40] 孙会玲,陈庆国,刘梅,等.天然材料贻贝壳的应用研究[J].安徽农业科学,2014,42(13):4069-4071,4101.
[41] The Commission of the European Communities.(EC) No 1881/2006 setting maximum levels for certain contaminants in foodstuffs[S].[S.l.]:Official Journal of the European Union,2006:L364/5-L364/24.
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