罗茜 , 陈伟 , 郑春田 , 林映才 , 王胜林 , 阮栋 , 王爽 , 夏伟光 , 邱月琴 , 熊云霞 . 家禽卵泡颗粒细胞体外培养方法研究进展[J]. 动物营养学报, 2016 , 28(9) : 2681 -2686 . DOI: 10.3969/j.issn.1006-267x.2016.09.003

[1] 林金杏.局部性促生长因子对鸡卵泡发育的调控及其机理的研究[D].博士学位论文.杭州:浙江大学,2011.
[2] 张昊,陈芳,梁振华,等.家禽卵泡闭锁研究进展[J].湖北畜牧兽医,2014,35(10):61-65.
[3] 黄华云,李春苗,黎寿丰,等.家禽卵泡发育研究进展[C]//中国畜牧兽医学会家禽学分会第九次代表会议暨第十六次全国家禽学术讨论会论文集.扬州:中国畜牧兽医学会,2013.
[4] YOON J D,JEON Y,CAI L,et al.Effects of coculture with cumulus-derived somatic cells on in vitro maturation of porcine oocytes[J].Theriogenology,2015,83(2):294-305.  
[5] FUKUI Y,ONO H.Effects of sera,hormones and granulosa cells added to culture medium for in-vitro maturation,fertilization,cleavage and development of bovine oocytes[J].Journal of Reproduction and Fertility,1989,86(2):501-506.  
[6] 金艳梅.鸡等级前卵泡颗粒细胞发育的激素和营养调控及机理研究[D].博士学位论文.杭州:浙江大学,2007.
[7] 王星,罗光彬,刘永,等.哺乳动物颗粒细胞凋亡研究进展[J].安徽农业科学,2007,35(12):3554-3555.
[8] 李彩萍.生殖激素对体外培养的水牛卵泡颗粒细胞凋亡的影响研究[D].硕士学位论文.南宁:广西大学,2006.
[9] GILBERT A B,EVANS A J,PERRY M M,et al.A method for separating the granulosa cells,the basal lamina and the theca of the preovulatory ovarian follicle of the domestic fowl (Gallus domesticus)[J].Journal of Reproduction and Fertility,1977,50(1):179-181.  
[10] ASEM E K,ZAKAR T,BIELLIER H V,et al.Progesterone production in granulosa cells of the domestic fowl:effects of incubation media,pH,cell density and some other factors[J].Domestic Animal Endocrinology,1984,1(3):235-249.  
[11] HAMMOND R W,TODD H,HERTELENDY F.Effects of mammalian gonadotropins on progesterone release and cyclic nucleotide production by isolated avian granulosa cells[J].General and Comparative Endocrinology,1980,41(4):467-476.  
[12] ASEM E K,FENG S L,STINGLEY-SALAZAR S R,et al.Basal lamina of avian ovarian follicle:influence on morphology of granulosa cells in-vitro[J].Comparative Biochemistry and Physiology Part C: Pharmacology Toxicology and Endocrinology,2000,125(2):189-201.  
[13] VANMONTFORT D,ROMBAUTS L,DECUYPERE E,et al.Source of immunoreactive inhibin in the chicken ovary[J].Biology of Reproduction,1992,47(6):977-983.  
[14] WEN R,HU S Q,XIAO Q H,et al.Leptin exerts proliferative and anti-apoptotic effects on goose granulosa cells through the PI3K/Akt/mTOR signaling pathway[J].The Journal of Steroid Biochemistry and Molecular Biology,2015,149:70-79.
[15] WOJTUSIK J,JOHNSON P A.Vitamin D regulates anti-Mullerian hormone expression in granulosa cells of the hen[J].Biology of Reproduction,2012,86(3):91.
[16] XIE Y L,PAN Y E,CHANG C J,et al.Palmitic acid in chicken granulosa cell death-lipotoxic mechanisms mediate reproductive inefficacy of broiler breeder hens[J].Theriogenology,2012,78(9):1917-1928.  
[17] ASEM E K,QIN W,RANE S G.Effect of basal lamina of ovarian follicle on T-and L-type Ca²⁺ currents in differentiated granulosa cells[J].American Journal of Physiology-Endocrinology and Metabolism,2002,282(1):E184-E196.
[18] WALTHER N,EINSPANIER A,JANSEN M.Expression of granulosa cell-specific genes and induction of apoptosis in conditionally immortalized granulosa cell lines established from H-2Kb-tsA58 transgenic mice[J].Biology of Reproduction,1999,60(5):1078-1086.  
[19] CHIANG M,STRONG J A,ASEM E K.Bovine serum albumin enhances calcium currents in chicken granulosa cells[J].Molecular and Cellular Endocrinology,1993,94(1):27-36.  
[20] PICTON H M,CAMPBELL B K,HUNTER M G.Maintenance of oestradiol production and expression of cytochrome P450 aromatase enzyme mRNA in long-term serum-free cultures of pig granulosa cells[J].Journal of Reproduction and Fertility,1999,115(1):67-77.  
[21] 丁海雷.鸡精原干细胞和支持细胞的分离培养与鉴定[D].硕士学位论文.扬州:扬州大学,2008.
[22] 叶婧,丁婷,杜小芳,等.不同分离方法对人卵巢颗粒细胞体外培养的影响[J].中国现代医学杂志,2013,23(17):1-5.
[23] 邓红惠,耿果霞,贺亚媚,等.猪三种不同直径卵泡中颗粒细胞的比较研究[J].家畜生态学报,2014,35(12):58-62.
[24] RANGEL P L,RODRÍGUEZ A,GUTIERREZ C G.Testosterone directly induces progesterone production and interacts with physiological concentrations of LH to increase granulosa cell progesterone production in laying hens (Gallus domesticus)[J].Animal Reproduction Science,2007,102(1/2):56-65.
[25] 郭小虎,张宏馨,陈辉,等.维生素E对蛋种鸡卵巢等级前颗粒细胞增殖的影响[J].中国家禽,2013,35(4):10-13.
[26] 熊建文,肖化,张镇西.MTT法和CCK-8法检测细胞活性之测试条件比较[J].激光生物学报,2007,16(5):559-562.
[27] TIAN Y,SHEN W,LAI Z W,et al.Isolation and identification of ovarian theca-interstitial cells and granulose cells of immature female mice[J].Cell Biology International,2015,39(5):584-590.  
[28] 杨永梅,陈娟,陈洋,等.哺乳动物卵泡颗粒细胞体外培养研究概况[J].中国畜牧兽医,2011,38(3):135-138.