圆斑星鲽幼鱼饲料中适宜的维生素C含量

王贞杰; 陈四清; 常青; 刘长琳; 胡建成; 严俊丽; 卢斌

doi:10.3969/j.issn.1006-267x.2016.10.015

动物营养学报 >

2016 , Vol. 28 >Issue 10: 3134 - 3144

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.015

水产营养 Aquaculture Nutrition

圆斑星鲽幼鱼饲料中适宜的维生素C含量

王贞杰 ,
陈四清 ,
常青 ,
刘长琳 ,
胡建成 ,
严俊丽 ,
卢斌

展开

1. 上海海洋大学水产与生命学院, 上海 201306;
2. 农业部海洋渔业资源可持续利用重点开放实验室, 中国水产科学研究院黄海水产研究所, 青岛 266071

收稿日期: 2016-04-24

网络出版日期: 2016-10-17

基金资助

山东省自主创新成果转化专项（2013ZHZX2A0803）；鳌山科技创新计划（2015ASKJ02-03）

收起

Optimal Dietary Vitamin C Content for Juvenile Spotted Halibut (Verasper variegates)

WANG Zhenjie ,
CHEN Siqing ,
CHANG Qing ,
LIU Changlin ,
HU Jiancheng ,
YAN Junli ,
LU Bin

Expand

1. College of Fishers and Life Sciences, Shanghai Ocean University, Shanghai 201306, China;
2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resource, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

Received date: 2016-04-24

Online published: 2016-10-17

Fold

摘要

本试验通过研究饲料中维生素C含量对圆斑星鲽幼鱼生长及消化、吸收、抗氧化、免疫功能的影响，以期得到圆斑星鲽幼鱼饲料中适宜的维生素C含量。试验在（12.5±1.5）℃的水温下进行，选择体重为（38.0±0.8）g的健康圆斑星鲽幼鱼，随机分为7组（每组3个重复，每个重复30尾），分别投喂维生素C含量为10.2、249.1、402.8、616.2、769.5、909.4和1 177.8 mg/kg的试验饲料。试验期为8周。结果表明：616.2 mg/kg维生素C组的增重率、饲料效率、摄食率均为最高，其增重率显著高于10.2和909.4 mg/kg维生素C组（P<0.05），饲料效率显著高于其他各组（P<0.05），摄食率显著高于除1 177.8 mg/kg维生素C组外的其他各组（P<0.05）。达到组织肝脏和肌肉最大维生素C积累量的饲料维生素C含量为769.5 mg/kg。616.2和769.5 mg/kg维生素C组的血清中丙二醛含量显著低于除402.8 mg/kg维生素C组外的其他各组（P<0.05）；402.8 mg/kg维生素C组的血清中过氧化氢酶和溶菌酶活力最高，显著高于其他各组（P<0.05）；402.8、616.2、769.4 mg/kg维生素C组血清中超氧化物歧化酶活力显著高于其他各组（P<0.05）。饲料维生素C含量为402.8、616.2或769.4 mg/kg时，胃和肠道蛋白酶、淀粉酶、脂肪酶均处在较高水平。饲料中维生素C含量在249.1~616.2 mg/kg时可提高肠道皱襞高度，增加肠壁厚度，促进肠道黏膜生长。结果显示：饲料中维生素C含量在402.8~616.2 mg/kg时能有效满足圆斑星鲽幼鱼的生长需求，并可提高抗氧化能力、非特异性免疫力和消化吸收能力。

关键词： 圆斑星鲽; 维生素C; 生长; 免疫; 抗氧化

本文引用格式

王贞杰 , 陈四清 , 常青 , 刘长琳 , 胡建成 , 严俊丽 , 卢斌 . 圆斑星鲽幼鱼饲料中适宜的维生素C含量[J]. 动物营养学报, 2016 , 28(10) : 3134 -3144 . DOI: 10.3969/j.issn.1006-267x.2016.10.015

Abstract

This experiment was conducted to study the effects of dietary vitamin C content on growth and the functions of digestion, absorption, antioxidant and immune of juvenile spotted halibut (Verasper variegates), in order to get the optimal dietary vitamin C content for juvenile spotted halibut. Water temperature was maintained at (12.5±1.5)℃ in this experiment. Juvenile spotted halibut with the body weight of (38.0±0.8) g were randomly assigned into 7 groups with 3 replicates per group and 30 fish per replicate, and fed 7 experimental diets with the vitamin C content were 10.2, 249.1, 402.8, 616.2, 769.5, 909.4 and 1 177.8 mg/kg, respectively. The experiment lasted for 8 weeks. The results showed as follows:the highest weight gain rate, feed efficiency ratio and feeding rate were found in 616.2 mg/kg vitamin C group. The weight gain rate of 616.2 mg/kg vitamin C group was significantly higher than that of 10.2 and 909.4 mg/kg vitamin C groups (P<0.05), the feed efficiency ratio of 616.2 mg/kg vitamin C group was significantly higher than that of other groups (P<0.05), and the feeding rate of 616.2 mg/kg vitamin C group was significantly higher than that of other groups except 1 177.8 mg/kg vitamin C group (P<0.05). The fish had the maximal liver and muscle vitamin C accumulation when dietary vitamin C content was 769.5 mg/kg. Serum malondialdehyde content of 616.2 and 769.5 mg/kg vitamin C groups was significantly lower than that of other groups except 402.8 mg/kg vitamin C group (P<0.05). The activities of serum catalase and lysozyme in 402.8 mg/kg vitamin C group had the highest values, which were significantly higher than those of other groups (P<0.05). Serum superoxide dismutase activity of 402.8, 616.2 and 769.4 mg/kg vitamin C groups was significantly higher than that of other groups (P<0.05). Protease, amylase and lipase activities in stomach and intestine were at a high level when dietary vitamin C content was 402.8, 616.2 or 769.4 mg/kg. Dietary vitamin C content within the scope of 249.1 to 616.2 mg/kg could promote the growth of the intestinal mucosa and increase the fold height and intestinal wall thickness. These results suggest that dietary vitamin C content within the scope of 402.8 to 616.2 mg/kg can effectively meet the need of growth, and improve the antioxidant capacity, non-specific immunity, digestion and absorption ability of juvenile spotted halibut.

Key words： juvenile spotted halibut (Verasper variegates); vitamin C; growth; immunity; antioxidant

参考文献

[1] 宋学宏,蔡春芳,潘新法,等.用生长和非特异性免疫力评定异育银鲫维生素C需要量[J].水产学报,2002,26(4):351-356.
[2] 沈同,王镜岩.生物化学[M].2版.北京:高等教育出版社,1995:365-367.
[3] 万金娟,刘波,戈贤平,等.日粮中不同水平维生素C对团头鲂幼鱼免疫力的影响[J].水生生物学报,2014,38(1):10-18.
[4] 周立斌,张伟,王安利,等.饲料维生素C对花鲈(Lateolabrax japonicus)幼鱼生长和免疫的影响[J].海洋与湖沼,2008,39(6):671-677.
[5] EO J,LEE K J.Effect of dietary ascorbic acid on growth and non-specific immune responses of tiger puffer,Takifugu rubripes[J].Fish & Shellfish Immunology,2008,25(5):611-616.

[6] 谢全森,李俊伟,杨振才.鲆鲽鱼类维生素及微量元素营养需求研究进展[J].现代农业科学,2008,15(12):5-7.
[7] 周歧存,刘永坚,麦康森,等.维生素C对点带石斑鱼(Epinephelus coioides)生长及组织中维生素C积累量的影响[J].海洋与湖沼,2005,35(2):152-158.
[8] 张道波,李爱杰,王如才.牙鲆对维生素C需求量的研究[J].饲料工业,2002,23(5):37-38.
[9] AI Q H,MAI K S,ZHANG C X,et al.Effects of dietary vitamin C on growth and immune response of Japanese seabass,Lateolabrax japonicus[J].Aquaculture,2004,242(1/2/3/4):489-500.
[10] 谢一荣,吴锐全,谢骏,等.维生素C对大口黑鲈生长与非特异性免疫的影响[J].南方水产,2006,2(3):40-45.
[11] 陈建明,叶金云,潘茜,等.饲料中添加维生素C对翘嘴鲌鱼种生长及组织中抗坏血酸含量的影响[J].中国水产科学,2007,14(1):106-112.
[12] AL-AMOUDI M M,EL-NAKKADI A M N,EL-NOUMAN B M.Evaluation of optimum dietary requirement of vitamin C for the growth of Oreochromis spilurus fingerlings in water from the Red Sea[J].Aquaculture,1992,105(2):165-173.

[13] OGUNTIBEJU O O.The biochemical,physiological and therapeutic roles of ascorbic acid[J].African Journal of Biotechnology,2008,7(25):4700-4705.
[14] DABROWSKI K,EL-FIKY N,KÖCK G,et al.Requirement and utilization of ascorbic acid and ascorbic sulfate in juvenile rainbow trout[J].Aquaculture,1990,91(3/4):317-337.
[15] 文杰.维生素E和维生素C生理功能的相互关系[J].国外畜牧科技,1995,22(1):6-9.
[16] 陈昌生,王淑红,纪德华,等.氨氮对九孔鲍过氧化氢酶和超氧化物歧化酶活力的影响[J].上海水产大学学报,2001,10(3):218-222.
[17] 周显青,李胜利,王晓辉,等.维生素C多聚磷酸酯对小鼠肝脏脂质过氧化物和抗氧化物酶的影响[J].动物学报,2004,50(3):370-374.
[18] 明建华,谢骏,徐跑,等.大黄素、维生素C及其配伍对团头鲂生长、生理生化指标、抗病原感染以及两种HSP70s mRNA表达的影响[J].水产学报,2010,34(9):1447-1459.
[19] 杨先乐.鱼类免疫学研究的进展[J].水产学报,1989,13(3):271-284.
[20] 刘树青,江晓路,牟海津,等.免疫多糖对中国对虾血清溶菌酶、磷酸酶和过氧化物酶的作用[J].海洋与湖沼,1999,30(3):278-283.
[21] 周歧存,丁燏,郑石轩,等.维生素C对凡纳滨对虾生长及抗病力的影响[J].水生生物学报,2004,28(6):592-598.
[22] 常青,梁萌青,王印庚,等.饲料维生素C对大菱鲆非特异性免疫力的影响[J].海洋水产研究,2005,26(5):22-26.
[23] 叶元土,林仕梅,罗莉,等.黄颡鱼消化能力与营养价值的研究[J].大连水产学院报,1997,12(2):23-30.
[24] 文泽平.泛酸对幼建鲤消化吸收功能和免疫功能的影响[D].硕士学位论文.雅安:四川农业大学,2008:1-80.
[25] 向阳.烟酸对幼建鲤消化能力和免疫功能的影响[D].硕士学位论文.雅安:四川农业大学,2008:1-71.
[26] 周景祥,余涛,黄权,等.鲤鱼、黄颡鱼和大眼鰤鲈消化酶活力的比较研究[J].吉林农业大学学报,2001,23(1):94-96,120.
[27] FARHANGI M,CARTER C G.Growth,physiological and immunological responses of rainbow trout (Oncorhynchus mykiss) to different dietary inclusion levels of dehulled lupin (Lupinus angustifolius)[J].Aquaculture Research,2001,32(S1):329-340.
[28] 章世元,徐建超,张杰,等.新型发酵豆粕对断奶仔猪生长性能及胃肠道发育的影响[J].畜牧与兽医,2009,41(7):20-23.
[29] UDA K,TSUJIKAWA T,FUJIYAMA Y,et al.Rapid absorption of luminal polyamines in a rat small intestine ex vivo model[J].Journal of Gastroenterology and Hepatology,2003,18(5):554-559.

[30] 申顺立.维生素C缺乏对幼建鲤器官发育和组织结构变化的影响[D].硕士学位论文.雅安:四川农业大学,2003:140.
[31] 池磊.不同来源的维生素C对幼建鲤消化、免疫和抗氧化功能影响的比较研究[D].硕士学位论文.雅安:四川农业大学,2009:33-40.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们