亚急性瘤胃酸中毒奶山羊瘤胃上皮形态结构和通透性的变化

程萌; 杨淑青; 高民; 谢天宇; 孙燕勇; 胡红莲

doi:10.3969/j.issn.1006-267x.2016.10.035

动物营养学报 >

2016 , Vol. 28 >Issue 10: 3311 - 3319

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.10.035

研究简报 Short Communications

亚急性瘤胃酸中毒奶山羊瘤胃上皮形态结构和通透性的变化

程萌 ,
杨淑青 ,
高民 ,
谢天宇 ,
孙燕勇 ,
胡红莲

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1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
2. 江苏蒙彼利生物科技有限公司, 常州 213100;
3. 内蒙古农牧业科学院动物营养与饲料研究所, 呼和浩特 010031;
4. 内蒙古圣牧高科牧业有限公司, 呼和浩特 010018

收稿日期: 2016-04-12

网络出版日期: 2016-10-17

基金资助

国家自然科学基金（31472124）；现代农业（奶牛）产业技术体系建设专项资金（CARS-37）

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Effects of Subacute Ruminal Acidosis on Ruminal Epithelial Morphology and Permeability in Dairy Goats

CHENG Meng ,
YANG Shuqing ,
GAO Min ,
XIE Tianyu ,
SUN Yanyong ,
HU Honglian

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1. College of Animal Sciences, Inner Mongolia Agricultural University, Huhhot 010018, China;
2. Jiangsu Mengbili Biological Technology Co., Ltd., Changzhou 213100, China;
3. Institute of Animal Nutrition and Feed, Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;
4. Inner Mongolia Holy Priest High-Tech Animal Husbandry Co., Ltd., Hohhot 010018, China

Received date: 2016-04-12

Online published: 2016-10-17

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摘要

本试验旨在研究亚急性瘤胃酸中毒（SARA）对瘤胃上皮形态结构和通透性的影响。选用体况良好、体重相近的泌乳期萨能奶山羊9只，随机分为3组（对照组、SARA组、恢复组，n=3），对照组饲喂基础饲粮[非纤维性碳水化合物与中性洗涤纤维比（NFC/NDF）=1.40]，SARA组和恢复组先后饲喂NFC/NDF为1.40、1.79、2.31、3.23的4种试验饲粮诱导SARA发生，每种饲喂15 d，恢复组奶山羊待SARA诱导成功后自由采食青干草30 d。对照组奶山羊分别在饲养30、60（与SARA组3只同时）和90 d（与恢复组3只同时）各屠宰1只。采集瘤胃腹囊部上皮组织用于石蜡切片、透射电子显微镜观察及尤斯灌流系统（Ussing chamber）研究。结果表明：1）组织切片结果显示，瘤胃上皮角质层厚度SARA组显著高于对照组和恢复组（P<0.05），恢复组显著低于对照组（P<0.05）；颗粒层厚度对照组显著高于SARA组和恢复组（P<0.05），但SARA组和恢复组之间无显著差异（P>0.05）；棘突层厚度3组之间无显著差异（P>0.05）；上皮总厚度恢复组显著低于对照组和SARA组（P<0.05），但对照组与SARA组之间无显著差异（P>0.05）。透射电子显微镜结果显示，SARA组瘤胃上皮紧密连接被破坏，细胞间隙增大，棘状层细胞线粒体出现降解并出现空泡。2）与对照组相比，SARA组和恢复组瘤胃上皮短路电流（Isc）、组织导电性（Gt）和辣根过氧化物酶（HRP）流速显著升高（P<0.05），跨膜电位差（PD）显著降低（P<0.05）。综合得出，SARA破坏了奶山羊瘤胃上皮形态结构的完整性，使瘤胃上皮通透性增加，导致瘤胃上皮屏障功能长期受损。

关键词： 亚急性瘤胃酸中毒; 瘤胃上皮; 形态结构; 超微结构; 通透性

本文引用格式

程萌 , 杨淑青 , 高民 , 谢天宇 , 孙燕勇 , 胡红莲 . 亚急性瘤胃酸中毒奶山羊瘤胃上皮形态结构和通透性的变化[J]. 动物营养学报, 2016 , 28(10) : 3311 -3319 . DOI: 10.3969/j.issn.1006-267x.2016.10.035

Abstract

The objective of this experiment was to study the effects of subacute ruminal acidosis (SARA) on ruminal epithelial morphology and permeability in dairy goats. Nine healthy lactating Saanen dairy goats with similar body weight were randomly divided into three groups (control group, SARA group and recovery group, n=3). Dairy goats in control group were fed a basal diet[non-fibre carbohydrates/neutral detergent fibre (none fiber carbon NFC/NDF)=1.40], those in SARA group and recovery group were fed experimental diets with different NFC/NDFs (1.40, 1.79, 2.31 and 3.23 in order, each for 15 days), which gradually induced SARA, and those in recovery group were ad libitum fed green hay for 30 days after SARA. One of 3 goats in control group were slaughtered on days 30, 60 (together with 3 goats in SARA group) and 90 d of feeding (together with 3 goats in recovery group), respectively. Samples of the ruminal epithelium from ventral sac were collected to make paraffin section, observe under transmission electron microscope and do Ussing chamber analysis. The results showed as follows:1) the results of histological slice revealed that the thickness of ruminal epithelial stratum corneum in SARA group was significantly higher than that in control and recovery groups (P<0.05), and recovery group was significantly lower than control group (P<0.05); the thickness of granulosum stratum in control group was significantly higher than that in SARA and recovery groups (P<0.05), but there was no significant difference between SARA group and recovery group (P>0.05); the thickness of spinosum stratum in three groups had no significant difference (P>0.05); the total thickness of the rumen epithelium in recovery group was significantly lower than that in control and SARA group (P<0.05), but there was no significant difference between control and SARA groups (P>0.05). The results of transmission electron microscope showed rumen epithelial tight junction structure in SARA group was destroyed and intercellular space was increased,and spinosum stratum cell mitochondria was degraded and showed vacuoles. 2) Compared with control group, the short-circuit, tissue conductance and the flux rate of horseradish peroxidase of rumen epithelium in SARA and recovery groups were significantly increased (P<0.05), and potential difference was significantly decreased (P<0.05). In conclusion, SARA impairs the integrity of ruminal epithelial morphological structure in dairy goats, and causes an increase in ruminal epithelial permeability, which results in rumen epithelial barrier function long-term damage.

Key words： subacute ruminal acidosis; rumen epithelium; morphology; ultramicrostructure; permeability

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