谷氨酸对脂多糖刺激断奶仔猪肠道能量代谢的影响

秦琴; 王秀英; 吴欢听; 朱惠玲; 刘玉兰

doi:10.3969/j.issn.1006-267x.2016.11.031

动物营养学报 >

2016 , Vol. 28 >Issue 11: 3618 - 3625

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.11.031

研究简报 Short Communications

谷氨酸对脂多糖刺激断奶仔猪肠道能量代谢的影响

秦琴 ,
王秀英 ,
吴欢听 ,
朱惠玲 ,
刘玉兰

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武汉轻工大学动物营养与饲料科学湖北省重点实验室, 武汉 430023

收稿日期: 2016-05-03

网络出版日期: 2016-11-18

基金资助

湖北省教育厅优秀中青年科技创新团队项目（T201508）

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Effects of Glutamate on Intestinal Energy Metabolism in the Lipopolysaccharide-Challenged Weaned Piglets

QIN Qin ,
WANG Xiuying ,
WU Huanting ,
ZHU Huiling ,
LIU Yulan

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Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2016-05-03

Online published: 2016-11-18

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摘要

本试验旨在研究谷氨酸（Glu）对脂多糖（LPS）刺激断奶仔猪肠道能量代谢的影响。选择24头断奶仔猪分为4个组，分别为对照组、LPS组、LPS+1.0% Glu组和LPS+2.0% Glu组，每组6个重复，每个重复1头猪。于试验第28天，试验组猪注射100 μg/kg BW LPS，对照组注射等量的生理盐水，4 h后屠宰，取肠道样品待测。结果表明：1）与对照组相比，LPS刺激导致断奶仔猪空肠三磷酸腺苷（ATP）、腺苷酸池（TAN）含量和能荷（EC）显著降低（P<0.05），一磷酸腺苷（AMP）/ATP值显著升高（P<0.05）；与LPS组相比，LPS+2.0% Glu组显著提高了空肠ATP、二磷酸腺苷（ADP）和TAN含量（P<0.05）。2）与对照组相比，LPS刺激导致断奶仔猪回肠柠檬酸合成酶和α-酮戊二酸脱氢酶系活性极显著降低（P<0.01），空肠α-酮戊二酸脱氢酶系活性有降低趋势（P=0.092）；与LPS组相比，除LPS+1.0% Glu组回肠柠檬酸合成酶活性显著降低（P<0.05）外，Glu对空肠和回肠三羧酸循环关键酶活性无显著影响（P>0.05）。3）与对照组相比，LPS刺激导致空肠过氧化物酶体增殖物激活受体γ辅激活因子1α（PGC1α）及回肠沉默信号调控因子1（Sirt1）和PGC1α的mRNA表达量极显著降低（P<0.01）；与LPS组相比，LPS+2.0% Glu组有提高空肠PGC1α（P=0.067）和回肠Sirt1（P=0.053）mRNA表达量的趋势，LPS+1.0% Glu组有提高回肠Sirt1 mRNA表达量的趋势（P=0.070）。由此可见，Glu可以改善LPS刺激导致的肠道能量损耗状态。

关键词： 谷氨酸; 脂多糖; 断奶仔猪; 肠道; 能量代谢

本文引用格式

秦琴 , 王秀英 , 吴欢听 , 朱惠玲 , 刘玉兰 . 谷氨酸对脂多糖刺激断奶仔猪肠道能量代谢的影响[J]. 动物营养学报, 2016 , 28(11) : 3618 -3625 . DOI: 10.3969/j.issn.1006-267x.2016.11.031

Abstract

This study was aimed to investigate the effects of glutamate (Glu) on intestinal energy metabolism in the lipopolysaccharide (LPS)-challenged weaned piglets. Twenty-four weaned pigs were assigned to four groups as control group, LPS group, LPS+1.0% Glu group and LPS+2.0% Glu group with 6 replicates each and 1 pig in per replicate. On the 28^th day of the trial, the piglets in the experimental groups were injected with 100 μg/kg BW LPS, and the piglets in the control group were injected with the same amount of 0.9% NaCl solution. At 4 h post-injection, pigs were slaughtered and intestinal samples were collected for further analysis. The results showed as follows:1) LPS challenge significantly decreased ATP and total adenine nucleotide (TAN) contents and energy charge (P<0.05), but significantly increased the ratio of AMP to ATP (P<0.05) in jejunum compared with the control group; LPS+2.0% Glu group significantly increased the contents of ATP, ADP and TAN (P<0.05) in jejunum compared with LPS group. 2) Compared with the control group, LPS challenge had a tendency to decrease the alpha-oxoglutarate dehydrogenase complex activity (P=0.092) in jejunum and extremely significantly decreased the activities of citrate synthase and alpha-oxoglutarate dehydrogenase complex (P<0.01) in ileum; compared with LPS group, Glu had no significant effects on the activities of tricarboxylic acid cycle key enzymes (P>0.05) in jejunum and ileum, except for the citrate synthase activity in ileum was significantly reduced in LPS+1.0% Glu group (P<0.05). 3) LPS challenge extremely significantly decreased the mRNA expressions of peroxisome proliferator-activated receptor gamma coactivator-1α (PGC1α) in jejunum and silent information regulator 1 (Sirt1) and PGC1α in ileum (P<0.01) compared with the control group; compared with LPS group, LPS+2.0% Glu group had a tendency to increase the mRNA expressions of PGC1α in jejunum (P=0.067) and Sirt1 in ileum (P=0.053), while LPS+1.0% Glu group had a tendency to increase the Sirt1 mRNA expression in ileum (P=0.070). These results indicate that dietary supplementation of Glu can improve energy loss status in LPS injured intestine.

Key words： glutamate; lipopolysaccharide; weaned piglets; intestine; energy metabolism

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