动物营养学报 >

2016 , Vol. 28 >Issue 11: 3634 - 3641

DOI: https://doi.org/10.3969/j.issn.1006-267x.2016.11.033

研究简报 Short Communications

饲粮粗蛋白质水平对猪胃肠道钙敏感受体基因表达、胃肠激素分泌及胃功能性酶活性的影响

  • 县怡涵 ,
  • 赵秀英 ,
  • 丁立人 ,
  • 孟祥龙 ,
  • 王超 ,
  • 朱伟云 ,
  • 杭苏琴
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  • 南京农业大学消化道微生物研究室, 南京 210095

收稿日期: 2016-05-12

  网络出版日期: 2016-11-18

基金资助

国家973项目（2013CB127301）资助

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Effects of Dietary Crude Protein Level on Calcium Sensing Receptor Gene Expression of Gastrointestinal Tract, Gastrointestinal Hormone Secretion and the Activities of Gastric Function Enzymes of Pigs

  • XIAN Yihan ,
  • ZHAO Xiuying ,
  • DING Liren ,
  • MENG Xianglong ,
  • WANG Chao ,
  • ZHU Weiyun ,
  • HANG Suqin
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  • Laboratory of Gastrointestinal Microbiology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2016-05-12

  Online published: 2016-11-18

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摘要

本试验旨在研究不同饲粮粗蛋白质水平对猪胃肠道钙敏感受体（CaSR）基因表达、胃肠激素分泌及胃功能性酶（H+-K+-ATP酶、胃蛋白酶）活性的影响，探讨小肠CaSR基因表达量与血清胃肠激素浓度以及胃CaSR基因表达量与H+-K+-ATP酶和胃蛋白酶活性的关系。选择35日龄、初始体重为（9.57±0.64）kg的“杜×长×大”杂交断奶仔猪18头，随机分为3组，分别为NP组（NRC标准粗蛋白质水平组）、MP组（较标准蛋白质水平组降低3%粗蛋白质水平）和LP组（较标准粗蛋白质水平组降低6%粗蛋白质水平），每组6个重复，每个重复1头仔猪。根据猪不同生长阶段的营养需要，分别于仔猪阶段（35~80日龄）饲喂粗蛋白质水平为20%（NP组）、17%（MP组）和14%（LP组）的饲粮，生长猪阶段（81~110日龄）饲喂粗蛋白质水平为18%（NP组）、15%（MP组）和12%（LP组）的饲粮，肥育猪阶段（111~160日龄）饲喂粗蛋白质水平为16%（NP组）、13%（MP组）和10%（LP组）的饲粮，平衡饲粮的赖氨酸（Lys）、蛋氨酸（Met）、苏氨酸（Thr）和色氨酸（Trp）水平，试验期125 d。试验结束后采集前腔静脉血液，屠宰全部试验猪后取胃食糜、胃、十二指肠、空肠和回肠组织及黏膜，测定血清胃肠激素浓度、胃食糜中胃蛋白酶和胃黏膜中H+-K+-ATP酶活性以及胃肠道各段组织中CaSR基因表达量。结果表明：1）LP组猪胃CaSR基因表达量显著高于NP和MP组（P<0.05），MP和LP组十二指肠及空肠CaSR基因表达量均显著低于NP组（P<0.05），而各组回肠CaSR基因表达量无显著差异（P>0.05）。2）与NP组相比，MP和LP组猪血清酪酪肽（PYY）和葡萄糖促胰岛素肽（GIP）浓度显著降低（P<0.05），MP组血清胆囊收缩素（CCK）浓度显著升高（P<0.05），LP组胃黏膜中H+-K+-ATP酶与胃食糜中胃蛋白酶活性显著升高（P<0.05）。3）胃CaSR基因表达量与胃黏膜中H+-K+-ATP酶和胃食糜中胃蛋白酶活性均呈显著正相关（P<0.05）；十二指肠CaSR基因表达量与血清GIP浓度呈显著正相关（P<0.05），与血清PYY浓度呈显著正相关趋势（0.05≤P < 0.10）；空肠CaSR基因表达量与血清GIP和PYY浓度均呈显著正相关（P<0.05）；回肠CaSR基因表达量与血清PYY浓度呈显著正相关趋势（0.05≤P < 0.10）。综上所述，饲粮粗蛋白质水平降低影响猪胃肠道CaSR基因的表达，从而影响胃功能性酶活性及胃肠激素分泌。

关键词： 猪; 粗蛋白质水平; 钙敏感受体; 胃肠激素; H+-K+-ATP酶; 胃蛋白酶

本文引用格式

县怡涵 , 赵秀英 , 丁立人 , 孟祥龙 , 王超 , 朱伟云 , 杭苏琴 . 饲粮粗蛋白质水平对猪胃肠道钙敏感受体基因表达、胃肠激素分泌及胃功能性酶活性的影响[J]. 动物营养学报, 2016 , 28(11) : 3634 -3641 . DOI: 10.3969/j.issn.1006-267x.2016.11.033

Abstract

The experiment was conducted to investigate the effects of different dietary crude protein (CP) levels on calcium sensing receptor (CaSR) gene expression of gastrointestinal tract, gastrointestinal hormone secretion and the activities of gastric function enzymes (pepsin and H+-K+-ATPase) of pigs, and to explore the relationships between CaSR gene expression level of small intestine and the concentration of gastrointestinal hormone in serum, CaSR gene expression level of stomach and the activities of H+-K+-ATPase and pepsin, respectively. Eighteen 35-day-old "Duroc×Landrace×Large White" crossed weaned pigs with initial body weight of (9.57±0.64) kg were randomly assigned into 3 group with 6 replicates per group and 1 pig per replicate, including NP group (NRC standard CP level group), MP group (decreased 3% CP level compared to the standard CP level group) and LP group (decreased 6% CP level compared to the standard CP level group). According to the nutrient needs of pigs in different growth periods, pigs were fed diets contained 20% (NP group), 17% (MP group) and 14% (LP group) CP levels in weaned pigs period (35 to 80 days of age), respectively; diets contained 18% (NP group), 15% (MP group) and 12% (LP group) CP levels in growing pigs period (81 to 110 days of age), respectively; diets contained 16% (NP group), 13% (MP group) and 10% (LP group) CP levels in finishing pigs period (111 to 160 days of age), respectively. The levels of dietary lysine (Lys), methionine (Met), threonine (Thr) and tryptophan (Trp) were balanced. The experimental period lasted for 125 days. Precaval vein blood were collected by the end of the experiment, then all pigs were slaughtered, and gastric chyme, stomach, duodenum, jejunum and ileum tissues and mucosa were collected, to determination of the concentration of gastrointestinal hormones in serum, the activities of pepsin of gastric chyme and H+-K+-ATPase of gastric mucosa and CaSR gene expression level of each tissues of the gastrointestinal tract. The results showed as follows:1) CaSR gene expression level of stomach of pigs in LP group was significantly higher than that in NP and MP groups (P<0.05), and CaSR gene expression level of duodenum and jejunum in MP and LP groups was significantly lower than that in NP group (P<0.05), but no significant difference was found in CaSR gene expression level of ileum among all groups (P>0.05). 2) Compared with NP group, the concentrations of peptide tyrosine tyrosine (PYY) and glucose insulinotropic peptide (GIP) in serum of pigs in MP and LP groups were significantly decreased (P<0.05), the concentration of cholecystokinin (CCK) in serum in MP group was significantly increased (P<0.05), and the activities of pepsin of gastric chyme and H+-K+-ATPase of gastric mucosa in LP group were significantly increased (P<0.05). 3) CaSR gene expression level in stomach had a significantly positive correlations with the activities of pepsin of gastric chyme and H+-K+-ATPase of gastric mucosa (P<0.05), CaSR gene expression level in duodenum had a significantly positive correlation with the concentration of GIP in serum (P<0.05), and had a positive correlation tendency with the concentration of PYY in serum (0.05≤P < 0.10), CaSR gene expression level in jejunum had a significantly positive correlations with the concentrations of GIP and PYY in serum (P<0.05), and CaSR gene expression level in ileum had a positive correlation tendency with the concentration of PYY in serum (0.05≤P < 0.10). In conclusion, reduction of dietary CP level affects CaSR gene expression of gastrointestinal tract, the activities of gastric function enzymes and gastrointestinal hormone secretion.

Key words： pigs; crude protein level; calcium sensing receptor; gastrointestinal hormone; H+-K+-ATPase; pepsin

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