动物营养学报 >

2017 , Vol. 29 >Issue 1: 118 - 126

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.01.014

反刍与草食动物营养

12月龄伊犁马对维生素B1和维生素B2的需要量

  • 陈俊宏 ,
  • 何雪曼 ,
  • 邓海峰 ,
  • 李晓斌 ,
  • 刘振 ,
  • 马军 ,
  • 甘建荣 ,
  • 唐雪梅 ,
  • 杨开伦
展开
  • 新疆农业大学新疆肉乳用草食动物营养重点实验室, 乌鲁木齐 830052

收稿日期: 2016-07-12

  网络出版日期: 2017-01-16

基金资助

“十二五”农村领域国家科技计划课题（2012BAD45B02）

收起

Requirements of Vitamin B1 and Vitamin B2 of 12-Month-Old Yili Horses

  • CHEN Junhong ,
  • HE Xueman ,
  • DENG Haifeng ,
  • LI Xiaobin ,
  • LIU Zhen ,
  • MA Jun ,
  • GAN Jianrong ,
  • TANG Xuemei ,
  • YANG Kailun
Expand
  • Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2016-07-12

  Online published: 2017-01-16

Fold

摘要

本试验旨在研究维生素B1、维生素B2不同摄入量对12月龄伊犁马血液、尿液相关指标的影响，以得到12月龄伊犁马对维生素B1、维生素B2的需要量。试验选取12月龄±5日龄、平均体重（245.28±18.36）kg的伊犁马公马35匹，随机分为5组，每组7匹，分别为试验Ⅰ组、试验Ⅱ组、试验Ⅲ组、试验Ⅳ组、试验Ⅴ组，马匹饲喂相同的基础饲粮，并分别添加0、16、32、48和64 mg/（匹·d）的维生素B1和0、10、20、30和40 mg/（匹·d）的维生素B2，进行为期20 d的饲养试验。试验Ⅰ组、试验Ⅱ组、试验Ⅲ组、试验Ⅳ组、试验Ⅴ组实际摄入维生素B1量分别为19.00、33.49、48.27、62.96、77.53 mg/（匹·d），实际摄入维生素B2量分别为21.95、31.68、41.77、51.53、61.26 mg/（匹·d）。结果表明：随着实际摄入维生素B1量的增加，伊犁马血浆维生素B1含量、红细胞转酮酶活性（E-TKA）、尿液维生素B1排出量逐渐升高，焦磷酸硫胺素（TPP）效应逐渐降低。试验Ⅰ组血浆维生素B1含量显著低于试验Ⅲ组（P<0.05），极显著低于试验Ⅳ组、试验Ⅴ组（P<0.01）；试验Ⅰ组E-TKA极显著低于试验Ⅲ组、试验Ⅳ组和试验Ⅴ组（P<0.01）；试验Ⅰ组TPP效应显著高于试验Ⅲ组（P<0.05），极显著高于试验Ⅳ组、试验Ⅴ组（P<0.01）；试验Ⅰ组尿液维生素B1排出量极显著低于试验Ⅲ组、试验Ⅳ组、试验Ⅴ组（P<0.01）。随着实际摄入维生素B2量的增加，伊犁马血浆维生素B2含量出现波动变化，红细胞谷胱甘肽还原酶活性系数（E-GRAC）逐渐降低，尿液维生素B2排出量逐渐增加。试验Ⅰ组血浆维生素B2含量显著低于试验Ⅲ组、试验Ⅳ组（P<0.05），极显著低于试验Ⅱ组、试验Ⅴ组（P<0.01）；试验Ⅰ组E-GRAC极显著高于试验Ⅱ组、试验Ⅲ组、试验Ⅳ组、试验Ⅴ组（P<0.01）；试验Ⅰ组尿液维生素B2排出量极显著低于试验Ⅲ组、试验Ⅳ组、试验Ⅴ组（P<0.01）。综合评价各指标，得到12月龄伊犁马对维生素B1的需要量为48.27 mg/（匹·d），对维生素B2的需要量为31.68 mg/（匹·d）。

关键词： 伊犁马; 维生素B1; 维生素B2; 需要量

本文引用格式

陈俊宏 , 何雪曼 , 邓海峰 , 李晓斌 , 刘振 , 马军 , 甘建荣 , 唐雪梅 , 杨开伦 . 12月龄伊犁马对维生素B1和维生素B2的需要量[J]. 动物营养学报, 2017 , 29(1) : 118 -126 . DOI: 10.3969/j.issn.1006-267x.2017.01.014

Abstract

In order to get the vitamin B1 and vitamin B2 requirements of Yili horses, the effects of different intakes of vitamin B1 and vitamin B2 on blood and urinary related indices of 12-month-old Yili horses were studied. Thirty-five healthy male Yili horses with the age of 12 months±5 days and the average body weight of (245.28±18.36) kg were randomly divided into 5 groups (trial groups Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ), and each group had 7 horses. The horses in trail groups Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ were fed the same basal diet added 0, 16, 32, 48 and 64 mg/(horse·d) of vitamin B1 and 0, 10, 20, 30 and 40 mg/(horse·d) of vitamin B2 for 20 days, respectively. The practical intakes of vitamin B1 and vitamin B2 in trail groups Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ were 19.00, 33.49, 48.27, 62.96, 77.53 mg/(horse·d) and 21.95, 31.68, 41.77, 51.53, 61.26 mg/(horse·d), respectively. The results showed as follows:with the practical intake of vitamin B1 increasing, plasma vitamin B1 content, erythrocyte transketolase activity (E-TKA) and urinary vitamin B1 output increased gradually, while the thiamin pyrophosphate effect (TPP-effect) decreased gradually. The plasma vitamin B1 content in trail group Ⅰ was significantly lower than that in trail group Ⅲ (P<0.05), and extremely significantly lower than that in trail groups Ⅳ and Ⅴ (P<0.01). The E-TKA in trail group Ⅰ was extremely significantly lower than that in trail groups Ⅲ, Ⅳ and Ⅴ (P<0.01). The TPP-effect in trail group Ⅰ was significantly higher than that in trail group Ⅲ (P<0.05), and extremely significantly higher than that in trail groups Ⅳ and Ⅴ (P<0.01). The urinary vitamin B1 output in trail group Ⅰ was extremely significantly lower than that in trail groups Ⅲ, Ⅳ and Ⅴ (P<0.01). With the practical intake of vitamin B2 increasing, the plasma vitamin B2 content showed a fluctuating change, the erythrocyte glutathione reductase activity coefficient (E-GRAC) decreased gradually, and the urinary vitamin B1 output increased gradually. The plasma vitamin B2 content in trail group Ⅰ was significantly lower than that in trail groups Ⅲ and Ⅳ (P<0.05), and extremely significantly lower than that in trail group Ⅴ (P<0.01). The E-GRAC in trail group Ⅰ was extremely significantly higher than that in trail groups Ⅱ, Ⅲ,Ⅳ and Ⅴ (P<0.01). The urinary vitamin B2 output in trail group Ⅰ was extremely significantly lower than that in trail groups Ⅲ, Ⅳ and Ⅴ (P<0.01). Base on the results of this study, the optimal requirements of vitamin B1 and vitamin B2 are 48.27 and 31.68 mg/(horse·d) for 12-month-old Yili horses, respectively.

Key words： Yili horses; vitamin B1; vitamin B2; requirement

参考文献

[1] 孙远明,余群力.食品营养学[M].北京:中国农业大学出版社,2002:95-100.
[2] MANZETTI S,ZHANG J,VAN DER SPOEL D.Thiamin function,metabolism,uptake,and transport[J].Biochemistry,2014,53(5):821-835.  
[3] MAKARCHIKOV A F,LAKAYE B,GULYAI I E,et al.Thiamine triphosphate and thiamine triphosphatase activities:from bacteria to mammals[J].Cellular and Molecular Life Sciences CMLS,2003,60(7):1477-1488.  
[4] MORIYAMA Y.Riboflavin transporter is finally identified[J].Journal of Biochemistry,2011,150(4):341-343.  
[5] EVANS E T R,EVANS W C,ROBERTS H E,et al.The treatment of a naturally occurring outbreak of bracken staggers in ponies[J].British Veterinary Journal,1951,107:399-411.
[6] CARROLL F D,GROSS H,HOWELL C E.The synthesis of B vitamins in the horse[J].Journal of Animal Science,1949,8(2):290-299.  
[7] TALLAKSEN C M E,BØHMER T,BELL H.Concomitant determination of thiamin and its phosphate esters in human blood and serum by high-performance liquid chromatography[J].Journal of Chromatography B:Biomedical Sciences and Applications,1991,564(1):127-136.  
[8] ROSER R L,ANDRIST A H,HARRINGTON W H,et al.Determination of urinary thiamine by high-pressure liquid chromatography utilizing the thiochrome fluorescent method[J].Journal of Chromatography B:Biomedical Sciences and Applications,1978,146(1):43-53.  
[9] 陈福华.饲料检验化验员手册[M].长沙:湖南科学技术出版社,2013:202-205.
[10] TAKEUCHI T,NISHINO K,ITOKAWA Y.Improved determination of transketolase activity in erythrocytes[J].Clinical Chemistry,1984,30(5):658-661.
[11] CAPO-CHICHI C D,GUÉANT J L,FEILLET F,et al.Analysis of riboflavin and riboflavin cofactor levels in plasma by high-performance liquid chromatography[J].Journal of Chromatography B:Biomedical Sciences and Applications,2000,739(1):219-224.  
[12] SHIBATA K,SUGITA C,SANO M,et al.Urinary excretion of B-group vitamins reflects the nutritional status of B-group vitamins in rats[J].Journal of Nutritional Science,2013,2:e12.
[13] 李丽花.硫胺素、核黄素和烟酰胺对肥胖大鼠能量代谢和空间学习记忆能力的影响[D].硕士学论文.青岛:青岛大学,2014.
[14] CYMBALUK N F,FRETZ P B,LOEW F M.Amprolium-induced thiamine deficiency in horses:clinical features[J].American Journal of Veterinary Research,1978,39(2):255-261.
[15] 潘晓花,王梦芝,付聪,等.饲粮精粗比和添加硫胺素对奶牛体外瘤胃发酵参数及菌群结构的影响[J].动物营养学报,2013,25(1):88-99.
[16] TALLAKSEN C M E,BØHMER T,BELL H.Blood and serum thiamin and thiamin phosphate esters concentrations in patients with alcohol dependence syndrome before and after thiamin treatment[J].Alcoholism:Clinical and Experimental Research,1992,16(2):320-325.  
[17] REINKEN L,STOLLEY H,DROESE W.Biochemical assessment of thiamine nutrition in childhood[J].European Journal of Pediatrics,1979,131(4):229-235.  
[18] ZIPORIN Z Z,NUNES W T,POWELL R C,et al.Thiamine requirement in the adult human as measured by urinary excretion of thiamine metabolites[J].The Journal of Nutrition,1965,85(3):297-304.
[19] HÜNSCHE K,WOLF P,LANGE G,et al.Investigations on adverse effects of feeding dried marsh horsetail (Equisetum palustre) to ruminants and horses[J].Proceedings of the Nutrition Society Physiol,2010,19(2):132-139.
[20] LONSDALE D.Red cell transketolase studies in a private practice specializing in nutritional correction[J].Journal of the American College of Nutrition,1988,7(1):61-67.  
[21] FUKUWATARI T,YOSHIDA E,TAKAHASHI K,et al.Effect of fasting on the urinary excretion of water-soluble vitamins in humans and rats[J].Journal of Nutritional Science and Vitaminology,2010,56(1):19-26.  
[22] MC DOWELL L R.Riboflavin[M]//MCDOWELL L R,CUNHA T J.Vitamins in animal nutrition:comparative aspects to human[M].Florida:Academic Press,1989:183-209.
[23] EUSSEN S J P M,VOLLSET S E,HUSTAD S,et al.Plasma vitamins B2,B6,and B12,and related genetic variants as predictors of colorectal cancer risk[J].Cancer Epidemiology Biomarkers & Prevention,2010,19(10):2549-2561.  
[24] HUSTAD S,MCKINLEY M C,MCNULTY H,et al.Riboflavin,flavin mononucleotide,and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation[J].Clinical Chemistry,2002,48(9):1571-1577.
[25] 陈金文,马振凯,顾景范.肉用仔鸡核黄素需要量的研究[J].营养学报,1990,12(4):349-354.
[26] BATES C J,PRENTICE A M,PAUL A A,et al.Riboflavin status in Gambian pregnant and lactating women and its implications for Recommended Dietary Allowances[J].The American Journal of Clinical Nutrition,1981,34(5):928-935.
[27] 郭长江,韦京豫,杨继军,等.我军重度劳动部队推荐的核黄素摄入量[J].解放军预防医学杂志,2010,28(4):238-241.
[28] LO C S.Riboflavin status of adolescent southern Chinese:riboflavin saturation studies[J].Human Nutrition Clinical Nutrition,1985,39(4):297-301.
[29] MADIGAN S M,TRACEY F,MCNULTY H,et al.Riboflavin and vitamin B6 intakes and status and biochemical response to riboflavin supplementation in free-living elderly people[J].The American Journal of Clinical Nutrition,1998,68(2):389-295.
[30] PRASAD A P,LAKSHMI A V,BAMJI M S.Interpretation of erythrocyte glutathione reductase activation test values for assessing riboflavin status[J].European Journal of Clinical Nutrition,1992,46(10):753-758.
[31] NRC.Nutrient requirements of horses[S].6th ed.WashingtonDC:National Academies Press,2007:296-299.
[32] FERRARIS R P,DIAMOND J.Crypt-villus site of glucose transporter induction by dietary carbohydrate in mouse intestine[J].The American Journal of Physiology,1992,262(61):1069-1073.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2