变温条件下不同投喂水平对施氏鲟幼鱼消化酶、代谢酶和抗氧化酶活性的影响

赵志刚; 李晋南; 徐奇友; 罗亮; 王常安; 王连生

doi:10.3969/j.issn.1006-267x.2017.01.015

动物营养学报 >

2017 , Vol. 29 >Issue 1: 127 - 133

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.01.015

水产营养

变温条件下不同投喂水平对施氏鲟幼鱼消化酶、代谢酶和抗氧化酶活性的影响

赵志刚 ,
李晋南 ,
徐奇友 ,
罗亮 ,
王常安 ,
王连生

展开

中国水产科学研究院黑龙江水产研究所, 哈尔滨 150070

收稿日期: 2016-06-27

网络出版日期: 2017-01-16

基金资助

国家自然科学基金项目（31302204）；黑龙江省自然基金项目（QC2015041）

收起

Effects of Different Ration Levels in Temperature Fluctuation on Digestive Enzyme, Metabolic Enzyme and Antioxidase Activities of Juvenile Amur Sturgeon (Acipenser schrenckii Brandt)

ZHAO Zhigang ,
LI Jinnan ,
XU Qiyou ,
LUO Liang ,
WANG Chang ,
WANG Liansheng

Expand

Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China

Received date: 2016-06-27

Online published: 2017-01-16

Fold

摘要

本试验旨在研究变温条件下不同投喂水平对施氏鲟幼鱼消化酶、代谢酶和抗氧化酶活性的影响。试验水温设恒温[（22.0±0.1）℃]和变温[（22±2）℃]2种模式，并根据投喂水平分为6组，分别为恒温100%饱食组（HW-100组）、变温100%饱食组（BW-100组）、变温90%饱食组（BW-90组）、变温80%饱食组（BW-80组）、变温70%饱食组（BW-70组）、变温60%饱食组（BW-60组）。将平均体重为（34.9±0.8）g的施氏鲟幼鱼随机分为6组，每组4个重复，每个重复15尾鱼，养殖周期为42 d。结果表明：变温条件下，在60%~80%范围内，随着投喂水平的降低，胃及瓣肠蛋白酶活性均显著升高（P<0.05）。与HW-100组相比，BW-60组与BW-70组的胃和瓣肠蛋白酶活性均显著升高（P<0.05）。变温条件下，十二指肠和瓣肠淀粉酶活性及瓣肠脂肪酶活性均随投喂水平的降低呈先升高后下降的趋势。BW-80组十二指肠淀粉酶活性显著高于HW-100组及BW-100组（P<0.05），BW-80组和BW-90组瓣肠淀粉酶活性显著高于其他各组（P<0.05）。所有变温投喂组瓣肠脂肪酶活性均显著低于HW-100组（P<0.05）。除BW-70组外，各变温投喂组血清丙氨酸转氨酶（ALT）活性均显著高于HW-100组（P<0.05）。与HW-100组相比，BW-60组、BW-70组血清超氧化物歧化酶（SOD）活性显著降低（P<0.05）。在变温条件下，在60%~90%范围内，鱼体血清总抗氧化能力（T-AOC）和黄嘌呤氧化酶（XOD）活性均随着投喂水平的降低整体呈先下降后上升的趋势，且均在投喂水平为80%时降至最低值。与HW-100组相比，BW-60组、BW-70组及BW-100组血清T-AOC显著升高（P<0.05），且BW-60组和BW-90组血清XOD活性显著升高（P<0.05）。综上所述，在周期性变温条件下，施氏鲟幼鱼可通过提高蛋白酶活性，即提高对饲料蛋白质消化效率的方式来补偿其对饲料蛋白质摄入的不足。与恒温100%饱食相比，变温条件下各投喂水平均可提高施氏鲟幼鱼血清ALT活性。

关键词： 变温; 投喂水平; 施氏鲟幼鱼; 消化酶; 代谢酶; 抗氧化酶

本文引用格式

赵志刚 , 李晋南 , 徐奇友 , 罗亮 , 王常安 , 王连生 . 变温条件下不同投喂水平对施氏鲟幼鱼消化酶、代谢酶和抗氧化酶活性的影响[J]. 动物营养学报, 2017 , 29(1) : 127 -133 . DOI: 10.3969/j.issn.1006-267x.2017.01.015

Abstract

The aim of this study was to investigate the effects of different ration levels in temperature fluctuation on digestive enzyme, metabolic enzyme and antioxidase activities of juvenile Amur sturgeon (Acipenser schrenckii Brandt). Two water temperatures were designed, one was constant temperature[(22.0±0.1)℃] and another was variable temperature[(22±2)℃]. Six groups according to different ration levels were arranged, and they were constant temperature 100% satiation group (HW-100 group), variable temperature 100% satiation group (BW-100 group), variable temperature 90% satiation group (BW-90 group), variable temperature 80% satiation group (BW-80 group), variable temperature 70% satiation group (BW-70 group) and variable temperature 60% satiation group (BW-60 group). Juvenile Amur sturgeon with the average body weight of (34.9±0.8) g were randomly divided into 6 groups with 4 replicates per group and 15 fish per replicate. The experiment lasted for 42 days. The results showed as follows:under the condition of temperature fluctuation, during 60% to 80% of ration levels, the protease activity of stomach and valvula intestine was significantly increased with ration level decreasing (P<0.05). The protease activity of stomach and valvula intestine in BW-60 group and BW-70 group was significantly higher than that in HW-100 group (P<0.05). Under the condition of temperature fluctuation, the amylase activity of duodenum and valvula intestine and the lipase activity of valvula intestine were firstly increased and then declined with ration level decreasing. The amylase activity of duodenum in BW-80 group was significantly higher than that in HW-100 group and BW-100 group (P<0.05), and the amylase activity of valvula intestine in BW-80 group and BW-90 group was significantly higher than that in other groups (P<0.05). The lipase activity of valvula intestine in all variable temperature groups was significantly lower than that in HW-100 group (P<0.05). Except BW-70 group, the serum alanine aminotransferase (ALT) activity in variable temperature groups was significantly higher than that in HW-100 group (P<0.05). Compared with HW-100 group, the serum superoxide dismutase (SOD) activity in BW-60 group and BW-70 group was significantly declined (P<0.05). Under the condition of temperature fluctuation, the serum total antioxidant capacity (T-AOC) and xanthine oxidase (XOD) activity were firstly declined and then rose with ration level decreased from 90% to 60%, and the lowest values of them appeared at 80% ration level. Compared with HW-100 group, the serum T-AOC in BW-60 group, BW-70 group and BW-100 group was significantly increased (P<0.05), and the serum XOD activity in BW-60 group and BW-90 group was significantly increased (P<0.05). In conclusion, under the condition of periodic temperature fluctuation, juvenile Amur sturgeon can compensate the shortage of feed protein by increasing the activity of protease, namely increasing feed protein digestion efficiency. All ration level in the temperature fluctuation condition can increase the serum ALT activity of juvenile Amur sturgeon compare with constant temperature 100% satiation.

Key words： variable temperature; ration level; juvenile Amur sturgeon (Acipenser schrenckii Brandt); digestive enzyme; metabolic enzyme; antioxidase

参考文献

[1] 黎军胜,李建林,吴婷婷.饲料成分与环境温度对奥尼罗非鱼消化酶活性的影响[J].中国水产科学,2004,11(6):585-588.
[2] ABUCAY J S,MAIR G C,SKIBINSKI D O F,et al.Environmental sex determination:the effect of temperature and salinity on sex ratio in Oreochromis niloticus L.[J].Aquaculture,1999,173(1/2/3/4):219-234.
[3] AZAZA M S,DHRAÏEF M N,KRAÏEM M M.Effects of water temperature on growth and sex ratio of juvenile Nile Tilapia Oreochromis niloticus (Linnaeus) reared in geothermal waters in southern Tunisia[J].Journal of Thermal Biology,2008,33(2):98-105.

[4] BARAS E,JACOBS B,MÉLARD C.Effect of water temperature on survival,growth and phenotypic sex of mixed (XX-XY) progenies of Nile tilapia Oreochromis niloticus[J].Aquaculture,2001,192(2/3/4):187-199.
[5] 袁伦强,谢小军,曹振东,等.温度对瓦氏黄颡鱼仔鱼发育的影响[J].西南师范大学学报,2005,30(2):312-315.
[6] 黄厚见.摄食水平、氨氮胁迫对梭鱼幼鱼生长的影响及其毒理效应研究[D].硕士学位论文.上海:上海海洋大学,2012.
[7] JOBLING M.Gastrointestinal overload-a problem with formulated feeds?[J].Aquaculture,1986,51(3/4):257-263.
[8] JENSEN A J.Growth of young migratory brown trout Salmo trutta correlated with water temperature in Norwegian rivers[J].Journal of Animal Ecology,1990,59(2):603-614.

[9] BARAS E,PRIGNON C,GOHOUNGO G,et al.Phenotypic sex differentiation of blue tilapia under constant and fluctuating thermal regimes and its adaptive and evolutionary implications[J].Journal of Fish Biology,2000,57(1):210-223.

[10] SIERRA E,DIAZ F,ESPINA S.Energy budget of Ictalurus punctatus exposed to constant and fluctuating temperatures[J].Riv Ita Acquacolt,1999,34(3):71-81.
[11] PILDITCH C A,GRANT J.Effect of temperature fluctuations and food supply on the growth and metabolism of juvenile sea scallops (Placopecten magellanicus)[J].Marine Biology,1999,134(2):235-248.

[12] MIAO S,TU S C.Modeling effect of thermic amplitude on growing Chinese shrimp,Penaeus chinensis (Osbeck)[J].Ecological Modelling,1996,88(1/2/3):93-100.
[13] 田相利,董双林,吴立新,等.恒温和变温下中国对虾生长和能量收支的比较[J].生态学报,2005,25(11):2811-2817.
[14] 董云伟,董双林,张美昭,等.变温对刺参幼参生长、呼吸代谢及生化组成的影响[J].水产学报,2005,29(5):659-665.
[15] 孙大江,曲秋芝,马国军,等.史氏鲟人工繁殖及养殖技术[M].北京:海洋出版社,2000.
[16] BRETT J R.Environmental factors and growth[M]//HOAR W S,RANDALL D J,BRETT J R.Fish Physiology.New York:Academic Press,1979:599-675.
[17] 白海文,张颖,李雪,等.温度对施氏鲟幼鱼摄食、生长和肠道消化酶活性的影响[J].中国水产科学,2012,19(5):799-805.
[18] 宋超,庄平,章龙珍,等.不同温度对西伯利亚鲟幼鱼生长的影响[J].海洋渔业,2014,36(3):239-246.
[19] 麦丽开,刘晓勇,潘鹏,等.水温对施氏鲟、小体鲟和西伯利亚鲟幼鱼生长的影响[J].水产学杂志,2014,27(4):15-22.
[20] 冯广朋,庄平,章龙珍,等.温度对中华鲟幼鱼血液生化指标的影响[J].生态学杂志,2010,29(10):1973-1978.
[21] 田宏杰,庄平,章龙珍,等.水温对施氏鲟幼鱼消化酶活力的影响[J].中国水产科学,2007,14(1):126-131.
[22] 冯广朋,庄平,章龙珍,等.温度对中华鲟幼鱼代谢酶和抗氧化酶活性的影响[J].水生生物学报,2012,36(1):137-142.
[23] HUNG S S O,LUTES P B.Optimum feeding rate of hatchery-produced juvenile white sturgeon (Acipenser transmontanus):at 20℃[J].Aquaculture,1987,65(3/4):307-317.
[24] HUNG S S O,LUTES P B,CONTE F S,et al.Growth and feed efficiency of white sturgeon (Acipenser transmontanus) sub-yearling at different feeding rates[J].Aquaculture,1989,80(1/2):147-153.
[25] FONTAINE P,GARDEUR J N,KESTEMONT P,et al.Influence of feeding level on growth,intraspecific weight variability and sexual growth dimorphism of Eurasian perch Perca fluviatilis L. reared in a recirculation system[J].Aquaculture,1997,157(1/2):1-9.
[26] STOREBAKKEN T,AUSTRENG E.Ration level for salmonids:Ⅰ.Growth,survival,body composition,and feed conversion in Atlantic salmon fry and fingerlings[J].Aquaculture,1987,60(3/4):189-206.
[27] BALLESTRAZZI B,LANARI D,D'AGARO E.Performance,nutrient retention efficiency,total ammonia and reactive phosphorus excretion of growing European sea-bass (Dicentrarchus labrax,L.) as affected by diet processing and feeding level[J].Aquaculture,1998,161(1/2/3/4):55-65.
[28] 刘云,孔伟丽,姜国良,等.2种免疫多糖对刺参组织主要免疫酶活性的影响[J].中国水产科学,2008,15(5):787-793.
[29] ZHANG R Q,CHEN Q X,ZHENG W Z,et al.Inhibition kinetics of green crab (Scylla serrata) alkaline phosphatase activity by dithiothreitol or 2-mercaptoethanol[J].The International Journal of Biochemistry & Cell Biology,2000,32(8):865-872.

[30] GIESEG S P,CUDDIHY S,HILL J V,et al.A comparison of plasma vitamin C and E levels in two Antarctic and two temperate water fish species[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2000,125(3):37l-378.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们