研究简报

凝结芽孢杆菌对肉鸡生长性能、免疫器官指数、血清生化指标及肠道菌群的影响

  • 赵娜 ,
  • 申杰 ,
  • 魏金涛 ,
  • 张巍 ,
  • 陈芳 ,
  • 杨雪海 ,
  • 郭万正 ,
  • 杜金平 ,
  • 黄少文
展开
  • 1. 湖北省农业科学院畜牧兽医研究所, 武汉 430064;
    2. 动物胚胎工程及分子育种湖北省重点实验室, 武汉 430064

收稿日期: 2016-07-21

  网络出版日期: 2017-01-16

基金资助

国家肉鸡产业技术体系(CARS-42-Z16);湖北省科技创新团队项目(2016-620-000-001-028)

Effects of Bacillus coagulans on Growth Performance, Immune Organ Indexes, Serum Biochemical Indexes and Intestinal Flora of Broilers

  • ZHAO Na ,
  • SHEN Jie ,
  • WEI Jintao ,
  • ZHANG Wei ,
  • CHEN Fang ,
  • YANG Xuehai ,
  • GUO Wanzheng ,
  • DU Jinping ,
  • HUANG Shaowen
Expand
  • 1. Institute of Animal Science and Veterinary Medicine, Hubei Academy of Agricultural Sciences, Wuhan 430064, China;
    2. Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Wuhan 430064, China

Received date: 2016-07-21

  Online published: 2017-01-16

摘要

本试验旨在研究凝结芽孢杆菌对肉鸡生长性能、免疫器官指数、血清生化指标及肠道菌群的影响。选择健康的1日龄AV500肉鸡10 000只,随机分成2组,每组5个重复,每个重复1 000只鸡。对照组饲喂基础饲粮,试验组饲喂基础饲粮+300 mg/kg凝结芽孢杆菌制剂。试验期6周。结果表明:1)1~21日龄、22~42日龄试验组肉鸡死淘率显著低于对照组(P<0.05),分别下降了61.7%、52.42%;22~42日龄,试验组肉鸡的平均日增重、平均日采食量均显著高于对照组(P<0.05)。2)21日龄试验组肉鸡的脾脏指数、胸腺指数、法氏囊指数均显著高于对照组(P<0.05),而肠道指数却显著低于对照组(P<0.05)。42日龄试验组肉鸡的脾脏指数、胸腺指数均显著高于对照组(P<0.05)。3)21和42日龄试验组肉鸡血清中的丙氨酸氨基转移酶、碱性磷酸酶活性及总蛋白、球蛋白含量均显著高于对照组(P<0.05)。4)21和42日龄试验组肉鸡盲肠、回肠食糜中凝结芽孢杆菌数量均显著高于对照组(P<0.05),大肠杆菌数量均显著低于对照组(P<0.05)。由此可见,饲粮中添加凝结芽孢杆菌可抑制肉鸡肠道中大肠杆菌的生长,提高免疫器官指数,降低死淘率。

本文引用格式

赵娜 , 申杰 , 魏金涛 , 张巍 , 陈芳 , 杨雪海 , 郭万正 , 杜金平 , 黄少文 . 凝结芽孢杆菌对肉鸡生长性能、免疫器官指数、血清生化指标及肠道菌群的影响[J]. 动物营养学报, 2017 , 29(1) : 249 -256 . DOI: 10.3969/j.issn.1006-267x.2017.01.028

Abstract

This study was conducted to evaluate the effects of Bacillus coagulans on growth performance, immune organ indexes, serum biochemical indexes and intestinal flora of broilers. A total of 10 000 one-day-old AV500 broilers were randomly divided into two groups with five replicates per group, and each replicate contained 1 000 broilers. Broilers in the control group were fed a basal diet, and the others in the test group were fed the basal diet+300 mg/kg Bacillus coagulans. The experiment lasted for 6 weeks. The results showed as follows:1) the mortality of broilers of test group was significantly lower than that of control group at 1 to 21 days of age and 22 to 42 days of age (P<0.05), and decreased by 61.7% and 52.42%, respectively; the average daily gain and average daily feed intake of broilers of test group were significantly higher than those of control group at 22 to 42 days of age (P<0.05). 2) The spleen index, thymus index and bursa of Fabricius index of broilers of test group were significantly higher than those of control group at 21 days of age (P<0.05), while the intestinal index was significantly lower than those of control group (P<0.05). The spleen index and thymus index of broilers of test group were significantly higher than those of control group at 42 days of age (P<0.05). 3) The activities of alanine aminotransferase, alkaline phosphatase and the contents of total protein, globulin in serum of broilers of test group were significantly higher than that of control group at 21 and 42 days of age (P<0.05). 4) The number of Bacillus coagulans in appendix and ileum digesta of broilers of test group was significantly higher than that of control group at 21 and 42 days of age (P<0.05), while the E. coli number was significantly lower than that of control group (P<0.05). In conclusion, dietary added with Bacillus coagulans can inhibit the growth of E. coli in the intestine, improve the immune organ indexes and reduce the mortality of broilers.

参考文献

[1] 刘虎传,张敏红,姜海龙.益生肠球菌的研究进展[J].动物营养学报,2011,23(12):2090-2096.
[2] DAS S,SEN R.Kinetic modeling of sporulation and product formation in stationary phase by Bacillus coagulans RK-02 vis-à-vis other Bacilli[J].Bioresource Technology,2011,102(20):9659-9667.  
[3] HYRONIMUS B,LE MARREC C,HADJ SASSI A,et al.Acid and bile tolerance of spore-forming lactic acid bacteria[J].International Journal of Food Microbiology,2000,61(2/3):193-197.
[4] 曹要玲,张弘,程敏婷,等.乳酸芽孢杆菌对动物生理环境和抗生素耐受性的试验研究[J].广东饲料,2011(9):19-20.
[5] 孙娜新,董玉兰,张根伟,等.鸡源凝结芽孢杆菌的分离鉴定及生物学特性研究[J].饲料研究,2014(5):60-63.
[6] 王标诗,李汴生,黄娟,等.超高静压协同中温对凝结芽孢杆菌芽孢灭活动力学规律的研究[J].微生物学通报,2008,35(4):633-638.
[7] 李雅丽,秦艳,周绪霞,等.6株芽孢杆菌的生物学特性比较研究[J].中国畜牧兽医,2011,38(4):62-66.
[8] ADAMI A,CAVAZZONI V.Occurrence of selected bacterial groups in the faeces of piglets fed with Bacillus coagulans as probiotic[J].Journal of Basic Microbiology,1999,39(1):3-9.  
[9] ZHOU X,WANG Y,GU Q,et al.Effect of dietary probiotic,Bacillus coagulans,on growth performance,chemical composition,and meat quality of Guangxi yellow chicken[J].Poultry Science,2010,89(3):588-593.  
[10] 周映华,吴胜莲,胡新旭,等.不同芽孢杆菌对断奶仔猪生产性能的影响[J].饲料工业,2012,33(3):21-23.
[11] 杨立华,赵述淼,冷一非,等.一株凝结芽孢杆菌产芽孢条件的研究[J].中国酿造,2010(5):96-98.
[12] INOOKA S,KIMURA M.The Effect of Bacillus natto in feed on the sheep red blood cell antibody response in chickens[J].Avian Diseases,1983,27(4):1086-1089.  
[13] HECK J X,FLÔRES S H,HERTZ P F,et al.Optimization of cellulase-free xylanase activity produced by Bacillus coagulans BL69 in solid-state cultivation[J].Process Biochemistry,2005,40(1):107-112.  
[14] 董惠钧,姜俊云,郑立军,等.新型微生态益生菌凝结芽孢杆菌研究进展[J].食品科学,2010,31(1):292-294.
[15] ARA K,MEGURO S,HASE T,et al.Effect of spore-bearing lactic acid-forming bacteria (Bacillus coagulans SANK 70258) administration on the intestinal environment,defecation frequency,fecal characteristics and dermal characteristics in humans and rats[J].Microbial Ecology in Health and Disease,2002,14(1):4-13.
[16] GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science,2010,129(1/2/3):95-103.
[17] MOOKIAH S,SIEO C C,RAMASAMY K,et al.Effects of dietary prebiotics,probiotic and synbiotics on performance,caecal bacterial populations and caecal fermentation concentrations of broiler chickens[J].Journal of the Science of Food and Agriculture,2014,94(2):341-348.  
[18] SEN S,INGALE S L,KIM T W,et al.Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance,nutrient retention,caecal microbiology and small intestinal morphology[J].Research in Veterinary Science,2012,93(1):264-268.  
[19] 曾丽莉,谢丽曲,陈婉如,等.凝结芽孢杆菌对樱桃谷肉鸭生长性能及生化指标的影响[J].福建农业学报,2015,30(5):435-441.
[20] HUNG A T,LIN S Y,YANG T Y,et al.Effects of Bacillus coagulans ATCC 7050 on growth performance,intestinal morphology,and microflora composition in broiler chickens[J].Animal Production Science,2012,52(9):874-879.  
[21] 宫秀燕,韦明,蒋秋斐,等.凝结芽孢杆菌对肠炎沙门氏菌感染肉鸡生产性能和抗氧化功能的影响[J].中国畜牧杂志,2015,51(17):74-79,98.
[22] 王政,张大伟,齐长海,等.凝结芽孢杆菌对白羽肉鸡生产性能及部分肠道微生物相关指标的影响[J].中国饲料,2016(7):31-34.
[23] 崔治中,崔保安.兽医免疫学[M].北京:中国农业出版社,2004:13-20.
[24] 吕景旭,王苏宁,黄秋实,等.肉仔鸡饲料中添加益生素的效果[J].中国饲料,1998(10):21-22.
[25] 关静姝,董经勇,张江.益生菌和抗菌肽复合物对肉鸡生产性能及免疫性能的影响[J].饲料博览,2016(3):19-22.
[26] RIVAS A L,FABRICANT J.Indications of immunodepression in chickens infected with various strains of Marek's disease virus[J].Avian Disease,1988,32(1):1-8.  
[27] 周淑芹,孙文志,魏树龙.酵母培养物对肉仔鸡生长性能和免疫机能的影响研究[J].饲料博览,2003(6):1-3.
[28] 何明清,程安春.动物微生态学[M].2版.成都:四川科学技术出版社,2004:201-208.
[29] 赵树平,包维臣,高鹏飞,等.凝结芽孢杆菌的特性及研究进展[J].家畜生态学报,2014,35(2):6-10,20.
[30] 罗毅,戴晋军.芽孢杆菌的作用原理及在蛋鸡中的应用[J].饲料研究,2009(4):37-38.
[31] FOSSATI P,ERGIS A M,ALLILAIRE J F.Executive functioning in unipolar depression:a review[J].L'Encephale,2002,28(2):97-107.
[32] 陈彤,杨小燕,祁保民.猪增生性肠炎消化系统酶活性的变化[J].安徽农业大学学报,2011,38(2):267-270.
[33] 肖英平,洪奇华,刘秀婷,等.谷氨酰胺对断奶仔猪生长性能、营养物质表观消化率、空肠碱性磷酸酶活性及与肠道健康相关因子基因表达的影响[J].动物营养学报,2012,24(8):1438-1446.
[34] 林丽花,柯芙容,詹湉湉,等.凝结芽孢杆菌对黄羽肉鸡生产性能、血清生化指标及抗氧化功能的影响[J].动物营养学报,2014,26(12):3806-3813.
[35] ŘEHULKA J,MINA ŘIK B.Effect of lecithin on the haematological and condition indices of the rainbow trout Oncorhynchus mykiss (Walbaum)[J].Aquaculture Research,2003,34(8):617-627.  
[36] 宋凯,单安山,李建平.不同配伍酶制剂添加于小麦日粮中对肉仔鸡生长和血液生化指标的影响[J].动物营养学报,2004,16(4):25-29.
[37] 张维娜,夏飞,谢骏,等.凝结芽孢杆菌JSSW-07对异育银鲫生长及免疫功能的影响[J].江苏农业学报,2015,31(3):630-637.
[38] 孙焕林,刘艳丰,王品,等.凝结芽孢杆菌对黄羽肉鸡生长性能、肠道功能及肉品质的影响[J].石河子大学学报:自然科学版,2014,32(3):307-312.
[39] 唐建中.芽孢杆菌抑菌耐药和耐热性能及其对黄羽肉鸡生产性能影响的研究[D].硕士学位论文.长沙:湖南农业大学,2010.
[40] HOLZAPFEL W H,BOTHA S J.Physiology of Sporolactobacillus strains isolated from different habitats and the indication of in vitro antagonism against Bacillus species[J].International Journal of Food Microbiology,1988,7(2):161-168.  
[41] LONKAR P,HARNE S D,KALOREY D R,et al.Isolation,in vitro antibacterial activity,bacterial sensitivity and plasmid profile of Lactobacilli[J].Asian-Australasian Journal of Animal Sciences,2005,18(9):1336-1342.  
[42] DONSKEY C J,HOYEN C K,DAS S M,et al.Effect of oral Bacillus coagulans administration on the density of vancomycin-resistant enterococci in the stool of colonized mice[J].Letters in Applied Microbiology,2001,33(1):84-88.  
[43] BONDI M,MESSI P,DANILA I,et al.Biological characteristics of LABLYS98,a Lactobacillus sporogenes for use as a probiotic compound[J].Industrie Alimentari,2000,39:704-710.
[44] 崔云龙,闫述翠,万阜昌.凝结芽胞杆菌TBC 169株对肠道致病菌的抑菌作用[J].中国微生态学杂志,2005,17(5):333-334,338.
[45] LE MARREC C,HYRONIMUS B,BRESSOLLIER P,et al.Biochemical and genetic characterization of coagulin,a new antilisterial bacteriocin in the pediocin family of bacteriocins,produced by Bacillus coagulans I4[J].Applied and Environmental Microbiology,2000,66(12):5213-5220.  
[46] MA K D,MAEDA T,YOU H Y,et al.Open fermentative production of L-lactic acid with high optical purity by thermophilic Bacillus coagulans using excess sludge as nutrient[J].Bioresource Technology,2014,151:28-35.
[47] JIANG T,XU Y B,SUN X C,et al.Kinetic characterization of recombinant Bacillus coagulans FDP-activated L-lactate dehydrogenase expressed in Escherichia coli and its substrate specificity[J].Protein Expression and Purification,2014,95:219-225.
文章导航

/