动物营养学报 >

2017 , Vol. 29 >Issue 2: 426 - 435

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.02.009

反刍动物营养 Ruminant nutrition

饲粮营养限制对羔羊肠道组织形态以及血清胰岛素样生长因子-1和胰高血糖素样-2浓度的影响

展开
  • 1. 中国农业科学院饲料研究所, 农业部饲料生物技术重点开放实验室, 北京 100081;
    2. 南京农业大学, 南京 210000
祁敏丽(1990-),女,河北保定人,硕士研究生,从事幼畜生理与营养研究。E-mail:minliqi@yeah.net

收稿日期: 2016-07-01

  网络出版日期: 2017-02-17

基金资助

公益性科研专项”南方地区幼龄草食畜禽饲养技术研究（201303143）；国家肉羊产业技术体系建设专项资金（CARS-39）

收起

Effects of Dietary Nutrition Restrictions on Intestinal Morphology and Serum Insulin-Like Growth Factor-1 and Glucagon-Like Peptide-2 Concentrations of Lambs

Expand
  • 1. Feed Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of the Ministry of Agriculture, Beijing 100081, China;
    2. Nanjing Agricultural University, Nanjing 210000, China

Received date: 2016-07-01

  Online published: 2017-02-17

Fold

摘要

本试验旨在研究饲粮营养限制对羔羊肠道组织形态及血清中胰岛素样生长因子-1（IGF-1）和胰高血糖素样-2（GLP-2）浓度的影响。选取64只17日龄湖羊羔羊随机分为4组，饲喂不同营养水平的代乳品和开食料，分别为对照（CON）组、20%蛋白质限制（PR）组、20%能量限制（ER）组、20%蛋白质+20%能量限制（BR）组，每组4个重复，每重复4只羔羊，公母各占1/2。于羔羊20日龄、40日龄和60日龄采集血清用于测定IGF-1和GLP-2的浓度，40日龄和60日龄时每组分别选取4只羔羊进行屠宰，屠宰后取十二指肠、空肠和回肠组织观察小肠组织形态结构。结果表明：1）PR组、ER组、BR组40日龄小肠重量显著低于CON组（P<0.05），到60日龄仅ER组羔羊小肠重显著低于CON组（P<0.05），小肠重量降低主要由于空肠重量的降低。2）PR组、ER组、BR组40日龄十二指肠、空肠绒毛高度及60日龄十二指肠绒毛高度显著低于CON组（P<0.05），ER组、BR组60日龄空肠绒毛高度显著低于CON组（P<0.05）。3）BR组40日龄十二指肠绒毛高度/隐窝深度显著低于CON组（P<0.05）。4）ER组60日龄血清GLP-2浓度显著低于其他3组（P<0.05），4组间血清IGF-1浓度无显著差异（P>0.05）。综上所述，饲粮营养限制抑制了羔羊十二指肠、空肠组织形态发育，同时能量限制可降低血清GLP-2的浓度。

关键词： 羔羊; 营养限制; 组织形态; 胰岛素样生长因子-1; 胰高血糖素样-2

本文引用格式

祁敏丽, 刁其玉, 马铁伟, 柴建民, 王波, 崔凯, 王杰, 张乃锋 . 饲粮营养限制对羔羊肠道组织形态以及血清胰岛素样生长因子-1和胰高血糖素样-2浓度的影响[J]. 动物营养学报, 2017 , 29(2) : 426 -435 . DOI: 10.3969/j.issn.1006-267x.2017.02.009

Abstract

The aim of this study was to assess the effects of dietary nutrition restrictions on intestinal morphology and serum insulin-like growth factor-1 (IGF-1) and glucagon-like peptide-2 (GLP-2) concentrations of lambs. Sixty-four 17-day-old Hu lambs were randomly divided into four groups with four replicates per group and four lambs per replicate (half male and half female), including control (CON) group, 20% of protein restriction (PR group), 20% of energy restriction (ER group), and 20% of energy+20% of protein restriction (BR group). Serum sample was collected at 20, 40 and 60 days of age to determine serum concentrations of GLP-2 and IGF-1. Four lambs of each group were slaughtered at 40 and 60 days of age, respectively, to observer morphological structure of duodenum, jejunum and ileum. The results showed as follows:1) the weight of small intestine was significantly reduced in PR, ER and BR groups compared with CON group at 40 days of age (P<0.05), and was significantly reduced only in ER group compared with CON group at 60 days of age(P<0.05). 2) Villus height of duodenum and jejunum at 40 days of age, and of duodenum at 60 days of age in PR, ER and BR groups was significantly lower than that of CON group (P<0.05), villus height of jejunum at 60 days of age in ER and BR groups was significantly lower than that of CON group (P<0.05). 3) Villus height/crypt depth (V/C) of duodenum at 40 days of age in BR group was significantly reduced compared with CON group (P<0.05). 4) Serum concentration of GLP-2 was significantly reduced in ER group compared with the other groups at 60 days of age (P<0.05), but serum IGF-1 concentration was not significantly differed in 4 groups (P>0.05). In conclusion, dietary nutrition restrictions reduce morphological development of duodenum and jejunum, and energy restriction reduces serum concentration of GLP-2 in lambs.

Key words： lamb; nutrition restriction; morphology; insulin-like growth factor-1; glucagon-like peptide-2

参考文献

[1] WEBSTER A J F.Energy costs of digestion and metabolism in the gut[M]//RUCKEBUSCH Y,THIVEND P. Digestive physiology and metabolism in ruminants.Netherlands:Springer,1980.

[2] SUN Z H,HE Z X,ZHANG Q L,et al.Effects of protein and/or energy restriction for six weeks on antioxidation capacity of plasma and gastrointestinal epithelial tissues of weaned kids[J].Livestock Science,2012,149(3):232-241.  

[3] MEYER A M,REED J J,NEVILLE T L,et al.Effects of nutritional plane and selenium supply during gestation on visceral organ mass and indices of intestinal growth and vascularity in primiparous ewes at parturition and during early lactation[J].Journal of Animal Science,2012,90(8):2733-2749.  

[4] REED J J,WARD M A,VONNAHME K A,et al.Effects of selenium supply and dietary restriction on maternal and fetal body weight,visceral organ mass and cellularity estimates,and jejunal vascularity in pregnant ewe lambs[J].Journal of Animal Science,2007,85(10):2721-2733.  

[5] TAYLOR-EDWARDS C C,BURRIN D G,MATTHEWS J C,et al.Expression of mRNA for proglucagon and glucagon-like peptide-2(GLP-2) receptor in the ruminant gastrointestinal tract and the influence of energy intake[J].Domestic Animal Endocrinology,2010,39(3):181-193.  

[6] TAYLOR-EDWARDS C C,BURRIN D G,HOLST J J,et al.Glucagon-like peptide-2(GLP-2) increases small intestinal blood flow and mucosal growth in ruminating calves[J].Journal of Dairy Science,2011,94(2):888-898.  

[7] YUNUSOVA R D,NEVILLE T L,VONNAHME K A,et al.Impacts of maternal selenium supply and nutritional plane on visceral tissues and intestinal biology in 180-day-old offspring in sheep[J].Journal of Animal Science,2013,91(5):2229-2242.  

[8] 杨诗兴,彭大惠,张文远,等.湖羊能量与蛋白质需要量的研究[J].中国农业科学,1988,21(2):73-80.

[9] 屠焰,刁其玉,岳喜新.一种0-3月龄羔羊的代乳品及其制备方法:中国,102894218A[P].2013-01-30.

[10] 王桂秋.营养水平对羔羊物质消化的影响及羔羊早期断奶时间的研究[D].硕士学位论文.北京:中国农业科学院,2005.

[11] 中华人民共和国农业部.NY/T 816-2004肉羊饲养标准[S].北京:中国农业出版社,2004.

[12] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.

[13] CANT J P,MCBRIDE B W,CROOM W J.The regulation of intestinal metabolism and its impact on whole animal energetics[J].Journal of Animal Science,1996,74(10):2541-2553.  

[14] LI D B,LIU X G,ZHANG C Z,et al.Effects of nutrient restriction followed by realimentation on growth,visceral organ mass,cellularity,and jejunal morphology in lambs[J].Livestock Science,2015,173:24-31.

[15] NELSON D L,COX M M.Lehninger:principles of biochemistry[M].4th ed.New York:W.H.Freeman,2005.

[16] MILLER B G,NEWBY T J,STOKES C R,et al.The importance of dietary antigen in the cause of postweaning diarrhea in pigs[J].American Journal of Veterinary Research,1984,45(9):1730-1773.

[17] EARLY R J,MAHGOUB O,LU C D.Energy and protein utilization for maintenance and growth in Omani ram lambs in hot climates.Ⅱ.Composition of tissue growth and nitrogen metabolism[J].Journal of Agricultural Science,2001,136(6):461-470.

[18] 王波,柴建民,王海超,等.蛋白质水平对湖羊双胞胎公羔生长发育及肉品质的影响[J].动物营养学报,2015,27(9):2724-2735.

[19] CATON J S,REED J J,AITKEN R P,et al.Effects of maternal nutrition and stage of gestation on body weight,visceral organ mass,and indices of jejunal cellularity,proliferation,and vascularity in pregnant ewe lambs[J].Journal of Animal Science,2008,87(1):222-235.  

[20] AZIM A,KHAN A G,ANJUM M I,et al.Effect of milk replacer and early weaning diets on growth performance of buffalo calves during weaning period[J].Pakistan Veterinary Journal,2011,31(1):23-26.

[21] SUN Z H,HE Z X,ZHANG Q L,et al.Effects of energy and protein restriction,followed by nutritional recovery on morphological development of the gastrointestinal tract of weaned kids[J].Journal of Animal Science,2013,91(9):4336-4344.  

[22] ZITNAN R,VOIGT J, KUHLA S,et al.Morphology of small intestinal mucosa and intestinal weight change with metabolic type of cattle[J].Veterinarni Medicina,2008,53(10):525-532.

[23] WANG Y J,HOLLIGAN S,SALIM H,et al.Effect of dietary crude protein level on visceral organ mass,cellularity,and the protein expression of ATP synthase,Na+/K+ATPase,proliferating cell nuclear antigen and ubiquitin in feedlot steers[J].Canadian Journal of Animal Science,2009,89(4):493-501.  

[24] MCLEOD K R,BALDWIN R L.Effects of diet forage:concentrate ratio and metabolizable energy intake on visceral organ growth and in vitro oxidative capacity of gut tissues in sheep[J].Journal of Animal Science,2000,78(3):760-770.  

[25] MONTANHOLI Y,FONTOURA A,SWANSON K,et al.Small intestine histomorphometry of beef cattle with divergent feed efficiency[J].Acta Veterinaria Scandinavica,2013,55(1):9.

[26] 闫云峰,杨华,杨永林,等.日粮不同蛋白质水平对绵羊IGF-1和GH分泌及基因表达的影响[J].畜牧兽医学报,2015,46(1):85-95.

[27] PELL J M,SAUNDERS J C,GILMOUR R S.Differential regulation of transcription initiation from insulin-like growth factor-Ⅰ (IGF-Ⅰ) leader exons and of tissue IGF-I expression in response to changed growth hormone and nutritional status in sheep[J].Endocrinology,1993,132(4):1797-1807.

[28] 张庆丽.早期能量与蛋白限制饲养对1月龄断奶羔羊胃肠道发育的影响[D].硕士学位论文.杨凌:西北农林科技大学,2010.

[29] LU J,ZHAO H,XU J,et al.Elevated cyclin D1 expression is governed by plasma IGF-1 through Ras/Raf/MEK/ERK pathway in rumen epithelium of goats supplying a high metabolizable energy diet[J].Journal of Animal Physiology and Animal Nutrition,2013,97(6):1170-1178.  

[30] BURRIN B,STOLL B, JIANG R H,et al.Minimal enteral nutrient requirements for intestinal growth in neonatal piglets:how much is enough?[J].American Journal of Clinical Nutrition,2000,71(6):1603-1610.

[31] BURRIN D,GUAN X F,STOLL B,et al.Glucagon-like peptide 2:a key link between nutrition and intestinal adaptation in neonates?[J].The Journal of Nutrition,2003,133(11):3712-3716.

[32] 王纯刚.鱼粉与丁酸钠对断奶仔猪生长、肠道发育和胰高血糖素样肽-2的影响[D].硕士学位论文.雅安:四川农业大学,2009.

[33] GÓRKA P,KOWALSKI Z M,PIETRZAK P,et al.Effect of method of delivery of sodium butyrate on rumen development in newborn calves[J].Journal of Dairy Science,2011,94(11):5578-5588.  

[34] 吴杰,齐珂珂,徐子伟,等.猪胰高血糖素样肽-2(pGLP-2)微球的制备及其对结肠炎小鼠肠道损伤修复的研究[J].农业生物技术学报,2014,22(2):150-157.

[35] JIN L,REYNOLDS L P,REDMER D A,et al.Effects of dietary fiber on intestinal growth,cell proliferation,and morphology in growing pigs[J].Journal of Animal Science,1994,72(9):2270-2278.
Options
文章导航

/

〈 〉

    联系我们

  • 地址：北京市海淀区圆明园西路2号
    　　　中国农业大学动科动医大楼153室
  • 邮编：100193
  • 电话：010－62817823
  • 网址：http://www.chinajan.com
  • E-mail：cjan@chinajan.com
京公网安备 11010802041926号    京ICP备17050989号-2