本试验旨在研究葡萄糖氧化酶（GOD）对生长獭兔营养物质利用、盲肠菌群结构及肠道消化酶活性的影响。选取40日龄生长獭兔150只，随机分为5组，每组设5个重复，每个重复6只（公母各占1/2）。对照组饲喂基础饲粮，4个试验组饲喂在基础饲粮的基础上分别添加0.1%、0.2%、0.3%和0.4% GOD的试验饲粮。预试期5 d，正试期30 d。结果表明：1）能量的表观消化率和表观消化能以对照组最低，0.4%组最高；各试验组粗纤维表观消化率较对照组有不同程度提高，其中0.4%组提高16.10%，差异显著（P<0.05）。2）0.4%组可消化氮、氮沉积量、氮利用率和氮生物利用率分别较对照组提高12.39%、17.48%、15.68%和12.78%，且均差异显著（P<0.05）。3）饲粮中添加GOD能够在一定程度上降低盲肠pH。与对照组相比，0.4%组盲肠乳酸菌和双歧杆菌数量显著提高（P<0.05），大肠杆菌数量显著降低（P<0.05）；0.3%组盲肠双歧杆菌数量显著提高（P<0.05）。4）0.4%组十二指肠部位胰蛋白酶活性显著高于对照组（P<0.05），空肠部位乳糖酶活性极显著高于对照组（P<0.01）。综合分析认为，饲粮中添加GOD能够改善饲粮中营养物质利用和獭兔肠道菌群结构，提高肠道消化酶活性，建议添加量为0.4%（60 U/kg）。

This paper was aimed to study the effects of glucose oxidase (GOD) on nutrient utilization, cecal microflora structure and intestinal digestive enzyme activities of growing Rex rabbits. A total of 150 growing Rex rabbits with the age of 40 days were randomly assigned to 5 groups with 5 replicates per group and 6 rabbits per replicate (half male and half female). The rabbits in control group were fed a basal diet, while the others in 4 experimental groups were fed the basal diet supplemented with 0.1%, 0.2%, 0.3% and 0.4% GOD, respectively. The trial lasted for 5 days for adaptation and 30 days for test. The results showed as follows:1) the apparent digestibility of energy and apparent digestible energy reached to the minimum in the control group, while reached to the maximum in the 0.4% group. Compared with the control group, crude fibre apparent digestibility of experimental groups were elevated more or less, and 0.4% group was significantly increased by 16.10% (P<0.05). 2) Compared with the control group, the digestible nitrogen, nitrogen retention, nitrogen utilization and nitrogen bioavailability of 0.4% group were significantly increased by 12.39%, 17.48%, 15.68% and 12.78%, respectively (P<0.05). 3) GOD addition could decrease the pH in caecum to a certain degree. Compared with the control group, the amounts of Lactobacilli and Bifidobacterium in caecum of 0.4% group were significantly increased (P<0.05), while the amount of Escherichia coli was significantly reduced (P<0.05); moreover, the amount of Bifidobacterium in caecum of 0.3% group was significantly increased (P<0.05). 4) Compared with the control group, the trypsin activity in duodenum of 0.4% group was significantly increased (P<0.05), and the lactase activity in jejunum was significantly increased (P<0.01). By synthesize analyses, diets with GOD can improve the intestinal microflora structure, digestive enzyme activities and nutrient utilization of Rex rabbits. Addition of 0.4% (60 U/kg) GOD is recommended.