饲料营养 Feed science and technology

爱拔益加肉仔鸡对胍基乙酸的耐受性评价

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  • 1. 中国农业大学动物营养学国家重点实验室, 农业部饲料安全与生物学效价重点实验室, 北京 100193;
    2. 北京君德同创农牧科技股份有限公司, 北京 100085
张德福(1976-),男,山东菏泽人,博士,研究方向为动物营养与饲料科学。E-mail:zhangdefu@gendone.com

收稿日期: 2016-07-18

  网络出版日期: 2017-02-17

基金资助

北京市科技计划项目资助(Z151100001215001)

Evaluation on Tolerance of Arbor Acres Broilers to Guanidinoacetic Acid

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  • 1. Ministry of Agriculture Feed Efficacy and Safety Evaluation Center, State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, China;
    2. Beijing Gendone Agricultural Technology Co., Ltd., Beijing 100085, China

Received date: 2016-07-18

  Online published: 2017-02-17

摘要

本试验旨在研究饲粮添加胍基乙酸(GAA)对爱拔益加(AA)肉仔鸡生长性能、血液学指标、脏器指数、组织同型半胱氨酸含量以及组织形态的影响,以系统评价AA肉仔鸡对GAA的耐受性。试验选用540只1日龄AA肉仔鸡公雏,随机分为5个组,每组6个重复,每个重复18只鸡。5组试鸡分别饲喂在基础饲粮中添加0、800、1 600、4 000和8 000 mg/kg GAA的饲粮。试验期42 d,分为前期(1~21日龄)和后期(22~42日龄)2个阶段。结果表明:与对照组相比,饲粮添加800~4 000 mg/kg GAA显著提高了肉仔鸡前期、后期和全期平均日增重(P<0.05),显著降低其后期和全期料重比(P<0.05),但对各期平均日采食量均无显著影响(P>0.05)。饲粮添加8 000 mg/kg GAA组的平均日增重、平均日采食量和料重比与对照组相比差异不显著(P>0.05)。饲粮添加800~8 000 mg/kg GAA对21、42日龄肉仔鸡血常规指标、血清生化指标、脏器指数和组织同型半胱氨酸含量均无显著影响(P>0.05)。饲粮添加4 000、8 000 mg/kg GAA对肉仔鸡肝脏和肾脏组织形态无不良影响。由此可见,肉仔鸡饲粮中添加GAA对其生长性能、血液学指标、脏器指数、组织同型半胱氨酸含量和组织形态无不良影响,肉仔鸡可耐受8 000 mg/kg GAA。

本文引用格式

张德福, 李军涛, 田耀耀, 杨立彬, 张丽英 . 爱拔益加肉仔鸡对胍基乙酸的耐受性评价[J]. 动物营养学报, 2017 , 29(2) : 456 -464 . DOI: 10.3969/j.issn.1006-267x.2017.02.013

Abstract

This experiment was conducted to study the effects of guanidinoacetic acid (GAA) supplementation on growth performance, haematological indice, organ indices, tissue homocysteine contents and histomorphology, and to systematically evaluate the tolerance of Arbor Acres (AA) broilers to GAA. A total of 540 one-day-old AA male broilers were randomly divided into five groups with six replicates and eighteen broilers per replicate. Broilers were fed the same basal diet supplemented with 0 (control), 800, 1 600, 4 000 and 8 000 mg/kg GAA for 42 days, respectively. The experimental period consisted of earlier stage (aged from 1 to 21 days) and later stage (aged from 22 to 42 days). The results showed that compared with control group, GAA supplementation at 800~4 000 mg/kg significantly increased average daily gain of broilers during earlier, later and overall periods (P<0.05), and significantly decreased the ratio of feed to gain during later and overall periods (P<0.05). However, average daily feed intake had no significant difference among groups during either period (P>0.05). GAA supplementation at 8 000 mg/kg did not significantly affect average daily gain, average daily feed intake, and the ratio of feed to gain (P>0.05). Dietary GAA supplementation had no significant effects on blood routine indices and serum biochemical parameters, organ indices, and tissue homocysteine content of broilers aged of 21 and 42 days (P>0.05). GAA supplementation at 4 000 and 8 000 mg/kg had no adverse effects on liver and kidney morphology of broilers. In conclusion, dietary GAA supplementation has no adverse effects on growth performance, haematological indices, organ indices, tissue homocysteine content and histomorphology of broilers. Broilers have a good tolerance to GAA at the level of 8 000 mg/kg.

参考文献

[1] WYSS M,KADDURAH-DAOUK R.Creatine and creatinine metabolism[J].Physiological Reviews,2000,80(3):1107-1213.

[2] MICHIELS J,MAERTENS L,BUYSE J,et al.Supplementation of guanidinoacetic acid to broiler diets:effects on performance,carcass characteristics,meat quality,and energy metabolism[J].Poultry Science,2012,91(2):402-412.  

[3] LEMME A,RINGEL J,STERK A,et al.Supplemental guanidino acetic acid affects energy metabolism of broilers[C]//Proceedings 16th European symposium on poultry nutrition.Strasbourg:[s.n.],2007:339-342.

[4] DE OLIVEIRA J E,UNI Z,FERKET P R.Important metabolic pathways in poultry embryos prior to hatch[J].World's Poultry Science Journal,2008,64(4):488-499.  

[5] 王连生,张圆圆,单安山.胍基乙酸的体内代谢及在动物生产中的应用[J].中国畜牧兽医,2010,37(6):13-16.

[6] BROSNAN J T,WIJEKOON E P,WARFORD-WOOLGAR L,et al.Creatine synthesis is a major metabolic process in neonatal piglets and has important implications for amino acid metabolism and methyl balance[J].The Journal of Nutrition,2009,139(7):1292-1297.  

[7] BAKER D H.Advances in protein-amino acid nutrition of poultry[J].Amino Acids,2009,37(1):29-41.  

[8] DA SILVA R P,CLOW K,BROSNAN J T,et al.Synthesis of guanidinoacetate and creatine from amino acids by rat pancreas[J].British Journal of Nutrition,2014,111(4):571-577.  

[9] MCGUIRE D M,GROSS M D,VAN PILSUM J F,et al.Repression of rat kidney L-arginine:glycine amidinotransferase synthesis by creatine at a pretranslational level[J].The Journal of Biological Chemistry,1984,259(19):12034-12038.

[10] MCBREAIRTY L E,ROBINSON J L,FURLONG K R,et al.Guanidinoacetate is more effective than creatine at enhancing tissue creatine stores while consequently limiting methionine availability in Yucatan miniature pigs[J].PLoS One,2015,10(6):e0131563.

[11] EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP).Safety and efficacy of guanidinoacetic acid for chickens for fattening,breeder hens and roosters,and pigs[J].EFSA Journal,2016,14(2):4394.

[12] 胡金良,陈宝玉,张德福,等.胍基乙酸在育肥猪饲养中的应用试验[J].广东畜牧兽医科技,2015,40(5):15-17.

[13] LIU Y,LI J L,LI Y J,et al.Effects of dietary supplementation of guanidinoacetic acid and combination of guanidinoacetic acid and betaine on postmortem glycolysis and meat quality of finishing pigs[J].Animal Feed Science and Technology,2015,205:82-89.

[14] MURAKAMI A E,RODRIGUEIRO R J,SANTOS T C,et al.Effects of dietary supplementation of meat-type quail breeders with guanidinoacetic acid on their reproductive parameters and progeny performance[J].Poultry Science,2014,93(9):2237-2244.  

[15] 洑琴,乔丽红,唐志刚,等.胍基乙酸对建鲤生产性能、体成分及肌肉能量代谢关键酶的影响[J].中国粮油学报,2015,30(3):85-89.

[16] TOSSENBERGER J,RADEMACHER M,NEMETH K,et al.Digestibility and metabolism of dietary guanidino acetic acid fed to broilers[J].Poultry Science,2016,95(9):2058-2067.  

[17] WALLIMANN T,WYSS M,BRDICZKA D,et al.Intracellular compartmentation,structure and function of creatine kinase isoenzymes in tissues with high and fluctuating energy demands:the ‘phosphocreatine circuit’ for cellular energy homeostasis[J].Biochemical Journal,1992,281(1):21-40.  

[18] ABUDABOS A M,SALEH F,LEMME A,et al.The relationship between guanidino acetic acid and metabolisable energy level of diets on performance of broiler chickens[J].Italian Journal of Animal Science,2014,13(3):548-556.

[19] DILGER R N,BRYANT-ANGELONI K,PAYNE R L,et al.Dietary guanidino acetic acid is an efficacious replacement for arginine for young chicks[J].Poultry Science,2013,92(1):171-177.  

[20] AYNSLEY-GREEN A,ALBERTI K G M M.In vivo stimulation of insulin secretion by guanidine derivatives in the rat[J].Hormone and Metabolic Research,1974,6(2):115-120.  

[21] EFSA.Safety and efficacy of guanidinoacetic acid as feed additive for chickens for fattening[J].The EFSA Journal,2009,7(3),doi:10.2903/j.efsa.2009.988.

[22] MEADOR C K,KREISBERG R A,FRIDAY J P,Jr,et al.Muscle mass determination by isotopic dilution of creatine-14C[J].Metabolism,1968,17(12):1104-1108.  

[23] 向丽华,陈燕萍,张智,等.24味有毒中药长期毒性实验对大鼠脏器指数的影响[J].中国中医基础医学杂志,2006,12(1):35-36.

[24] STEAD L M,AU K P,JACOBS R L,et al.Methylation demand and homocysteine metabolism:effects of dietary provision of creatine and guanidinoacetate[J].American Journal of Physiology:Endocrinology and Metabolism,2001,281(5):E1095-E1100.

[25] SETOUE M,OHUCHI S,MORITA T,et al.Hyperhomocysteinemia induced by guanidinoacetic acid is effectively suppressed by choline and betaine in rats[J].Bioscience,Biotechnology,and Biochemistry,2008,72(7):1696-1703.  

[26] OSTOJIC S M,NIESS B,STOJANOVIC M,et al.Co-administration of methyl donors along with guanidinoacetic acid reduces the incidence of hyperhomocysteinaemia compared with guanidinoacetic acid administration alone[J].British Journal of Nutrition,2013,110(5):865-870.  
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