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不同品种猪在不同生理阶段骨骼肌葡萄糖转运载体和胰岛素受体表达的发育性变化

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  • 华南农业大学动物科学学院, 广州 510642
刘文佐(1991-),男,湖北仙桃人,硕士研究生,从事动物营养与饲料科学研究。E-mail:lwz698631@sina.com

收稿日期: 2016-07-28

  网络出版日期: 2017-02-17

基金资助

国家重点基础研究发展计划项目(2012CB124701);广东省科技计划项目(2016A020210104);广州市科技计划项目(201510010258)

Ontogenetic Regulation of Glucose Transporter and Insulin Receptor mRNA Expression in Skeletal Muscle of Different Kinds of Pigs at Different Physiological Stages

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  • College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2016-07-28

  Online published: 2017-02-17

摘要

本试验旨在研究不同品种猪在不同生理阶段骨骼肌葡萄糖转运载体(GLUT)1、GLUT4和胰岛素受体(IR)mRNA表达的发育规律。试验以1、7、30、60、90和150日龄蓝塘和长白两品种猪背最长肌、半膜肌和半腱肌组织样品cDNA为模板,采用实时荧光半定量PCR的方法,检测猪GLUT1GLUT4 mRNA在不同骨骼肌中的表达模式。结果显示:1)1~150日龄蓝塘和长白猪背最长肌、半膜肌和半腱肌GLUT1 mRNA表达存在相似的发育变化规律,在1日龄时相对表达丰度最高。2)1~150日龄蓝塘和长白猪半膜肌和半腱肌GLUT4 mRNA相对表达丰度随日龄呈波形式变化,变化速度和程度存在品种差异。1日龄和150日龄时蓝塘猪背最长肌GLUT4 mRNA相对表达丰度低于同日龄长白猪(P>0.05),而在其余日龄时高于同日龄长白猪(P>0.05)。3)蓝塘和长白猪背最长肌、半膜肌和半腱肌IR mRNA相对表达丰度在1和30日龄时表现出较高水平,而在7、60、90和150日龄时无显著差异(P>0.05);同日龄蓝塘和长白猪之间IR mRNA相对表达丰度无显著性差异(P>0.05)。结果表明:猪骨骼肌GLUT1 mRNA的表达在不同生理阶段存在差异,猪骨骼肌GLUT4 mRNA的表达在不同品种和生理阶段存在显著差异,且其与IR mRNA的表达相关性较弱,无明显规律。

本文引用格式

刘文佐, 左建军, 代发文, 叶慧, 曹庆云, 周响艳, 邹仕庚, 黄志毅, 职爱民, 冯定远 . 不同品种猪在不同生理阶段骨骼肌葡萄糖转运载体和胰岛素受体表达的发育性变化[J]. 动物营养学报, 2017 , 29(2) : 605 -612 . DOI: 10.3969/j.issn.1006-267x.2017.02.029

Abstract

This experiment was conducted to determine the ontogenetic regulation of glucose transporter (GLUT) 1, GLUT4 and insulin receptor (IR) mRNA expression in skeletal muscle of different kinds of pigs at different physiological stages. Lantang (LT) and Landrace (LD) pigs at the age of 1, 7, 30, 60, 90 and 150 days were selected to determine the expression patterns of GLUT1 and GLUT4 at different skeletal muscle tissues including longissimus dorsi muscles (LM), semimembranosus muscles (SM) and semitendinosus muscles (SD) by the method of real-time semiquantitative PCR for cDNA templates. The results showed as follows:1) LT and LD pigs exhibited similar development of mRNA expressions of GLUT1 in the LM, SM and SD from 1 to 150 days of age with the highest level at the age of 1 day. 2) LT and LD pigs exhibited waveform variations of mRNA relative expression abundance of GLUT4 from 1 to 150 days of age with breed differences in the speed and extend of variations. At the age of 1 and 150 days, the mRNA relative expression abundance of GLUT4 in the LM of LT pig was lower than that of LD pig with the same age (P>0.05), while was higher than that of LD pig with the same age of the rest days (P>0.05). 3) LT and LD pigs exhibited higher level of the mRNA relative expression abundance of IR in the LM, SM and SD at the age of 1 and 30 days, while there were not significant difference among 7, 60, 90 and 150 days of age (P>0.05). There was no significant difference in mRNA relative expression abundance of IR between LT and LD pigs with the same ages (P>0.05). The results suggest that GLUT1 mRNA expression in skeletal muscles of pigs can be regulated by physiological stages, while GLUT4 mRNA expression is not only differentially regulated by breeds but also differentially distributed in physiological stages, and the expression level of GLUT4 is weakly related to the IR mRNA expression and shows no apparent rules.

参考文献

[1] JOOST H G,BELL G I,BEST J D,et al.Nomenclature of the GLUT/SLC2A family of sugar/polyol transport facilitators[J].American Journal of Physiology:Endocrinology and Metabolism,2002,282(4):E974-E976.

[2] HALL J R,SHORT C E, DRIEDZIC W R.Sequence of Atlantic cod (Gadus morhua) GLUT4,GLUT2 and GPDH:developmental stage expression,tissue expression and relationship to starvation-induced changes in blood glucose[J].Journal of Experimental Biology,2006,209:4490-4502.

[3] KHAN J M,WINGERTZAHN M A,TEICHBERG S,et al.Development of the intestinal SGLT1 transporter in rats[J].Molecular Genetics and Metabolism,2000,69(3):233-239.  

[4] BARFULL A,GARRIGA C,MITJANS M,et al.Ontogenetic expression and regulation of Na+-D-glucose cotransporter in jejunum of domestic chicken[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2002,282(3):G559-G564.

[5] 王修启,谭会泽,束刚,等.不同基因型肉鸡十二指肠SGLT1和GLUT2 mRNA表达的发育性变化[J].农业生物技术学报,2006,14(3):334-340.

[6] TURK E,KERNER C J,LOSTAO M P,et al.Membrane topology of the human Na+/glucose cotransporter SGLT1[J].Journal of Biological Chemistry,1996,271(4):1925-1934.  

[7] DYER J,WOOD I S,PALEJWALA A,et al.Expression of monosaccharide transporters in intestine of diabetic humans[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2002,282(2):G241-G248.

[8] WRIGHT E M.The intestinal Na+/glucose cotransporter[J].Annual Review of Physiology,1993,55(1):575-589.  

[9] HODIN R A,CHAMBERLAIN S M,MENG S.Pattern of rat intestinal brush-border enzyme gene expression changes with epithelial growth state[J].American Journal of Physiology,1995,269(2):C385-C391.

[10] HODIN R A,GRAHAM J R,MENG S,et al.Temporal pattern of rat small intestinal gene expression with refeeding[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,1994,266(1):G83-G89.

[11] GARRIGA C,ROVIRA N,MORETÓ M,et al.Expression of Na+-D-glucose cotransporter in brush-border membrane of the chicken intestine[J].American Journal of Physiology Regulatory,Integrative and Comparative Physiology,1999,276(2):R627-R631.

[12] 王修启,张兆敏,张磊,等.日粮添加木聚糖酶对肉鸡小肠葡萄糖吸收及其转运载体基因表达影响[J].农业生物技术学报,2005,13(4):497-502.

[13] 王修启,谭会泽,束刚,等.鸡肠道SGLT1和GLUT2 mRNA表达的组织特异性研究[J].畜牧兽医学报,2006,37(1):12-17.

[14] HUMPHREY B D,STEPHENSEN C B,CALVERT C C,et al.Glucose and cationic amino acid transporter expression in growing chickens (Gallus gallus domesticus)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2004,138(4):515-525.  

[15] SKLAN D.Fat and carbohydrate use in posthatch chicks[J].Poultry Science,2003,82(1):117-122.  

[16] DOS SANTOS J M,BENITE-RIBEIRO S A,QUEIROZ G,et al.The effect of age on glucose uptake and GLUT1 and GLUT4 expression in rat skeletal muscle[J].Cell Biochemistry and Function,2012,30(3):191-197.  

[17] BANDA M,BOMMINENI A,THOMAS R A,et al.Evaluation and validation of housekeeping genes in response to ionizing radiation and chemical exposure for normalizing RNA expression in real-time PCR[J].Mutation Research/Genetic Toxicology and Environmental Mutagenesis,2008,649(1/2):126-134.

[18] GASTER M,HANDBERG A,BECK-NIELSEN H,et al.Glucose transporter expression in human skeletal muscle fibers[J].American Journal of Physiology:Endocrinology and Metabolism,2000,279(3):E529-E538.

[19] SWEAZEA K L,BRAUN E J.Glucose transporter expression in English sparrows (Passer domesticus)[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2006,144(3):263-270.  

[20] DUEHLMEIER R,SAMMET K,WIDDEL A,et al.Distribution patterns of the glucose transporters GLUT4 and GLUT1 in skeletal muscles of rats (Rattus norvegicus),pigs (Sus scrofa),cows (Bos taurus),adult goats,goat kids (Capra hircus),and camels (Camelus dromedarius)[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2007,146(2):274-282.  

[21] GARDAN D,GONDRET F,LOUVEAU I.Lipid metabolism and secretory function of porcine intramuscular adipocytes compared with subcutaneous and perirenal adipocytes[J].American Journal of Physiology:Endocrinology and Metabolism,2006,291(2):E372-E380.

[22] HOLMAN G D,SANDOVAL I V.Moving the insulin-regulated glucose transporter GLUT4 into and out of storage[J].Trends in Cell Biology,2001,11(4):173-179.  

[23] HE J,THAMOTHARAN M,DEVASKAR S U.Insulin-induced translocation of facilitative glucose transporters in fetal/neonatal rat skeletal muscle[J].American Journal of Physiology:Endocrinology and Metabolism,2003,284(1):R1138-R1146.

[24] HOU J C,PESSIN J E.Ins (endocytosis) and outs (exocytosis) of GLUT4 trafficking[J].Current Opinion in Cell Biology,2007,19(4):466-473.  

[25] FILHO H C M,MCKEEVER K H,GORDON M E,et al.Novel findings regarding GLUT-4 expression in adipose tissue and muscle in horses-a preliminary report[J].The Veterinary Journal,2007,174(3):565-569.  

[26] OTANI K,HAN D H,FORD E L,et al.Calpain system regulates muscle mass and glucose transporter GLUT4 turnover[J].Journal of Biological Chemistry,2004,279(20):20915-20920.  

[27] SILVA J L T,GIANNOCCO G,FURUYA D T,et al.NF-κB,MEF2A,MEF2D and HIF1-a involvement on insulin- and contraction-induced regulation of GLUT4 gene expression in soleus muscle[J].Molecular and Cellular Endocrinology,2005,240(1/2):82-93.

[28] SERAPHIM P M,NUNES M T,GIANNOCCO G,et al.Age related obesity-induced shortening of GLUT4 mRNA poly(A) tail length in rat gastrocnemius skeletal muscle[J].Molecular and Cellular Endocrinology,2007,276(1/2):80-87.
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