不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响

祝倩, 马思聪, 姬玉娇, 印遇龙, 孔祥峰

doi:10.3969/j.issn.1006-267x.2017.02.030

动物营养学报 >

2017 , Vol. 29 >Issue 2: 613 - 619

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.02.030

研究简报 Short communications

不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响

展开

1. 中国科学院亚热带农业生态研究所, 亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术研究中心, 农业部中南动物营养与饲料科学观测实验站, 长沙 410125;
2. 中国科学院环江喀斯特农业生态实验站, 香猪研究中心, 环江 547100

祝倩(1992-),女,河南周口人,硕士研究生,动物营养与饲料科学专业。E-mail:walanzhuqian@163.com

收稿日期: 2016-08-01

网络出版日期: 2017-02-17

基金资助

国家自然科学基金面上项目（31270044，31572421）；中科院“西部之光”人才培养计划重点项目

收起

Effects of Different Nitrogen/Energy Diets on Expression of Genes Related to Umbilical Vessel Development of Pregnant Huanjiang Mini-Pigs

Expand

1. Key Laboratory of Agro-Ecological Processes in Subtropical Region, Hunan Provincial Engineering Research Center of Healthy Livestock, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
2. Research Center of Mini-Pig, Huanjiang Observation and Research Station for Karst Ecosystems, Chinese Academy of Sciences, Huanjiang 547100, China

Received date: 2016-08-01

Online published: 2017-02-17

Fold

摘要

本试验旨在研究不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响。选用首次妊娠的环江香猪48头，根据体重随机分为2组，每组8个重复（栏），每个重复3头猪。配种后分别饲喂高氮能比饲粮[消化能（DE）为14.73 MJ/kg，粗蛋白质（CP）含量为13.11%，氮能比为0.89]和低氮能比饲粮（DE为12.24 MJ/kg，CP含量为9.77%，氮能比为0.80）。分别于妊娠第45、75和110天每栏取1头母猪，处死屠宰，收集每窝中最大体重、平均体重和最小体重胎猪对应的脐带，利用实时荧光定量PCR方法检测血管发育相关基因——成纤维细胞生长因子（FGF）、血管内皮生长因子（VEGF）、血管内皮生长因子受体2（VEGFR2）和内皮型一氧化氮合酶（eNOS）的表达量。结果表明：与低氮能比饲粮组相比，高氮能比饲粮显著上调了脐带血管发育相关基因的表达量（P<0.05），包括妊娠45 d最大体重胎猪的VEGF和VEGFR2表达量，平均体重胎猪的VEGF表达量；妊娠75 d最大体重胎猪的VEGF、VEGFR2和eNOS表达量，平均体重胎猪的VEGF和eNOS表达量，最小体重胎猪的VEGFR2表达量；妊娠110 d最大体重胎猪的FGF、VEGFR2和eNOS表达量，平均体重胎猪的FGF和VEGFR2表达量，最小体重胎猪的VEGF和eNOS表达量。不同妊娠期相同标准体重、同一妊娠期不同标准体重胎猪的脐带血管发育相关基因的表达量均存在差异。由此可见，高氮能比饲粮可能由于其蛋白质和氨基酸水平较高而显著上调了妊娠环江香猪脐带血管发育相关基因的表达量，不同妊娠期或同一妊娠期不同标准体重胎猪的脐带血管发育相关基因的表达量也存在差异。

关键词： 环江香猪; 妊娠; 饲粮氮能比; 脐带; 血管发育

本文引用格式

祝倩, 马思聪, 姬玉娇, 印遇龙, 孔祥峰 . 不同氮能比饲粮对妊娠环江香猪脐带血管发育相关基因表达的影响[J]. 动物营养学报, 2017 , 29(2) : 613 -619 . DOI: 10.3969/j.issn.1006-267x.2017.02.030

Abstract

This study was conducted to investigate the effects of different nitrogen/energy diets on expression of genes related to umbilical vessel development of pregnant Huanjiang mini-pigs. A total of 48 primiparous Huanjiang mini-pigs were randomly assigned into two groups according to the body weight (BW), and reared in eight replicates (pens) with three mini-pigs per replicate. After service, the animals were fed with high nitrogen/energy diet (digestible energy was 14.73 MJ/kg, crude protein content was 13.11%, nitrogen/energy was 0.89) and low nitrogen/energy diet (digestible energy was 12.24 MJ/kg, crude protein content was 9.77%, nitrogen/energy was 0.80), respectively. At 45, 75 and 110 d of gestation, one pig was randomly selected and sacrificed from each pen. The umbilical cord tissues were collected from three fetuses with maximal BW, average BW and minimal BW per litter. The expression levels of fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor 2 (VEGFR2) and endothelial nitric oxide synthase (eNOS), which related to umbilical vessel development, were detected by RT-qPCR technique. The results showed that compared with the low nitrogen/energy diet group, the high nitrogen/energy diet significantly increased the expression levels of genes related to umbilical vessel development (P<0.05), including VEGF and VEGFR2 genes expression levels of fetus at 45 d of gestation with maximal BW; VEGF gene expression level of fetus at 45 d of gestation with average BW; VEGF, VEGFR2 and eNOS genes expression levels of fetus at 75 d of gestation with maximal BW; VEGF and eNOS genes expression levels of fetus at 75 d of gestation with average BW, VEGFR2 gene expression level of fetus at 75 d of gestation with minimal BW; FGF, VEGFR2 and eNOS genes expression levels of fetus at 110 d of gestation with maximal BW, FGF and VEGFR2 genes expression levels of fetus at 110 d of gestation with average BW, and VEGF and eNOS genes expression levels of fetus at 110 d of gestation with minimal BW. The expression levels of umbilical vessel genes of fetuses in different gestation stages with the same standard BW were different, as well as of fetuses with different standard BW in a same gestation stage. These findings suggest that the high nitrogen/energy diet containing higher levels of protein and amino acids can significantly increase the expression levels of genes related to the umbilical vessel development in pregnant Huanjiang mini-pigs, and the tested gene expression levels are different during different gestation stages, as well as among the fetuses with different standard body weights during a same gestation stage.

Key words： Huanjiang mini-pigs; pregnancy; dietary nitrogen/energy ratio; umbilical cord; blood vessel development

参考文献

[1] 胡建红,岳国璋,杜芳,等.营养调控对母猪繁殖性能的影响[J].畜牧兽医杂志,2011,30(6):43-44.

[2] SFERRUZZI-PERRI A N,CAMN E J.The programming power of the placenta[J].Frontiers in Physiology,2016,7:33.

[3] FISHER S J.Why is placentation abnormal in preeclampsia?[J].American Journal of Obstetrics & Gynecology,2015,213(Suppl.4):S115-S122.

[4] WU S P,XU X W,LI C C,et al.Six placenta permeability-related genes:molecular characterization and expression analysis in pigs[J].Animal,2009,3(3):408-414.

[5] BOLEHOVSAKÁ P,SEHNAL B,DRIÁK D,et al.Changes in placental angiogenesis and their correlation with foetal intrauterine restriction[J].Ceska Gynekologie,2015,80(2):144-150.

[6] 孔祥峰,伍国耀,印遇龙.猪宫内生长迟缓及其防治研究进展[J].畜牧与兽医,2009,41(10):96-101.

[7] 孔祥峰,印遇龙,伍国耀.猪功能性氨基酸营养研究进展[J].动物营养学报,2009,21(1):1-7.

[8] 刘俊锋,吴琛,孔祥峰,等.精氨酸对妊娠环江香猪胎儿生长发育的影响[J].中国农业科学,2011,44(5):1040-1045.

[9] HE J T,DONG L,XU W,et al.Dietary tributyrin supplementation attenuates insulin resistance and abnormal lipid metabolism in suckling piglets with intrauterine growth retardation[J].PLoS One,2015,10(8):e0136848.

[10] XU W,BAI K W,HE J T,et al.Leucine improves growth performance of intrauterine growth retardation piglets by modifying gene and protein expression related to protein synthesis[J].Nutrition,2016,32(1):114-121.

[11] 石彩霞,王海荣.营养基因组学在动物营养与饲料科学研究中的应用[J].饲料研究,2014(1):13-15,22.

[12] 祝倩,姬玉娇,李华伟,等.高、低营养水平饲粮对妊娠环江香猪繁殖性能、体成分和血浆生化参数的影响[J].动物营养学报,2016,28(5):1534-1540.

[13] 张莲英,苏家联,韦朝阳,等.环江香猪和巴马香猪生产情况调查[J].养殖与饲料,2011(10):5-7.

[14] JOHNSTON L,TROTTIER N.Nutritional methods to improve sow productivity examined[J].Feedstuffs,1999,25:12-17.

[15] HALDER J B,ZHAO X,SOKER S,et al.Differential expression of VEGF isoforms and VEGF₁₆₄-specific receptor neuropilin-1 in the mouse uterus suggests a role for VEGF164 in vascular permeability and angiogenesis during implantation[J].Genesis,2006,26(3):213-224.

[16] 许英蕾,孙建义.成纤维细胞生长因子与其受体的研究进展[J].药物生物技术,2004,11(3):194-198.

[17] 严宏祥,黄小燕,徐来仁.妊娠母猪的精氨酸营养与胎儿发育[J].饲料广角,2010(7):26-27,31.

[18] 谭兵兵,王乾兴,谭晓珊,等.胎盘绒毛血管生成状况及VEGF、MMP-2和MMP-9表达与早期自然流产的关系[J].生殖与避孕,2006,26(8):477-482.

[19] SHIBUYA M.Vascular endothelial growth factor and its receptor system:physiological functions in angiogenesis and pathological roles in various diseases[J].Journal of Biochemistry,2013,153(1):13-19.

[20] 高成,闫小毅,卓巍,等.胚胎发育中血管新生的研究[J].中国细胞生物学学报,2015,37(5):720-727.

[21] CHEN F,WANG T J,FEGN C P,et al.Proteome differences in placenta and endometrium between normal and intrauterine growth restricted pig fetuses[J].PLoS One,2015,10(11):e0142396.

[22] DEMIR R,SEVAL Y,HUPPERTZ B.Vasculogenesis and angiogenesis in the early human placenta[J].Acta Histochemica,2007,109(4):257-265.

[23] LU Y,XIONG Y,HUO Y Q,et al.Grb-2-associated binder 1(Gab1) regulates postnatal ischemic and VEGF-induced angiogenesis through the protein kinase A-endothelial NOS pathway[J].Proceedings of the National Academy of Sciences of the United States of America,2011,108(7):2957-2962.

[24] 范辉,胡雅兵,王小红,等.丹红注射液对重症急性胰腺炎大鼠胰腺组织ET-1、eNOS和iNOS基因表达的影响[J].世界华人消化杂志,2009,17(27):2784-2790.

[25] VALDÉS G,CORTHORN J.The angiogenic and vasodilatory utero-placental network[J].Placenta,2011,32(Suppl. 2):S170-S175.

[26] WU X,YIN Y L,LIU Y Q,et al.Effect of dietary arginine and N-carbamoylglutamate supplementation on reproduction and gene expression of eNOS,VEGFA and PlGF1 in placenta in late pregnancy of sows[J].Animal Reproduction Science,2012,132(3/4):187-192.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们