动物营养学报 >

2017 , Vol. 29 >Issue 2: 620 - 627

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.02.031

研究简报 Short communications

茶皂素对奶牛瘤胃原虫区系的影响

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  • 1. 北京农学院动物科学技术学院, 奶牛营养学北京市重点实验室, 北京 102206;
    2. 中国农业科学院畜牧研究所, 北京 100193
赵士萍(1990-),女,云南腾冲人,硕士研究生,研究方向为反刍动物营养。E-mail:1243830639@qq.com

收稿日期: 2016-08-01

  网络出版日期: 2017-02-17

基金资助

“十二五”国家科技支撑项目（25012BAD14B09）；“十三五”国家重大科技专项（2016YFDO70020，2016YFDO700205）；北京市农业局北京市现代农业产业技术体系奶牛创新团队

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Effects of Tea Saponin on Rumen Protozoa Flora of Dairy Cows

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  • 1. Key Laboratory for Dairy Cow Nutrition of Beijing, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2016-08-01

  Online published: 2017-02-17

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摘要

本研究旨在研究茶皂素对奶牛瘤胃原虫区系的影响。选取12头健康荷斯坦奶牛[体重（550±30）kg，产奶量35 kg/（头·d），胎次2~4]，随机分为4组，每组3头，均饲喂基础饲粮，分别于晨饲前灌服0（对照）、20、30、40 g/（头·d）的茶皂素。试验期49 d，其中预试期14 d，正试期35 d，正试期内每7 d于晨饲前1 h用口腔采样器采集瘤胃液。采用聚丙烯酰氨凝胶电泳（DGGE）结合18S rDNA序列分析技术研究瘤胃原虫区系的变化。结果表明：1）添加茶皂素可以选择性地抑制瘤胃中原虫的生长。2）与对照组相比，30、40 g/（头·d）茶皂素组的丰富度指数和香农多样性指数都显著降低（P<0.05），优势度指数显著增加（P<0.05），但是均一性指数无显著变化（P>0.05）；茶皂素显著减少了瘤胃中前庭亚纲内毛目的原虫数量。综上所述，饲粮中添加30、40 g/（头·d）茶皂素均可抑制奶牛瘤胃原虫的生长，并且降低瘤胃原虫的多样性。

关键词： 奶牛; 原虫区系; 茶皂素

本文引用格式

赵士萍, 严淑红, 蒋琦晖, 常肖肖, 王炳, 方洛云, 蒋林树, 熊本海 . 茶皂素对奶牛瘤胃原虫区系的影响[J]. 动物营养学报, 2017 , 29(2) : 620 -627 . DOI: 10.3969/j.issn.1006-267x.2017.02.031

Abstract

The objective of this study was to research the effects of tea saponin on rumen protozoa flora of dairy cows. Twelve healthy Holstein dairy cows[body weight was (550±30) kg; milk yield was 35 kg/(head·d); 2 to 4 parities] were randomly allocated to one of four groups with 3 cows per group. Tea saponin[0 (control), 20, 30 and 40 g/(head·d)] was perfused to cows before morning feeding. The experiment lasted for 49 d with 14 d of adaptation period and 35 d of formal test period. Rumen fluid was collected by mouth sampler at 1 h before morning feeding every 7 d during formal test period. Denaturing gradient gel electrophoresis (DGGE) combined with 18S rDNA sequence analysis technique was used for rumen protozoa flora analysis. The results showed as follows:1) tea saponin could selectively inhibit protozoa growth. 2) Compared with control group, richness index and Shannon index in 30 and 40 g/(head·d) tea saponin groups were significantly reduced (P<0.05), and dominance index was significantly increased (P<0.05), but evenness index was not significantly different among groups (P>0.05); tea saponin could significantly reduce rumen Vestibuliferia Entodiniomorphida protozoa number. In conclusion, dietary supplementation of 30 and 40 g/(head·d) tea saponin can inhibit protozoa growth in rumen of dairy cows, and reduce the diversity of rumen protozoa.

Key words： dairy cow; protozoa flora; tea saponin

参考文献

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