本试验旨在研究松针多糖对正常状态及脂多糖（LPS）刺激状态下小鼠腹腔巨噬细胞的免疫调节作用。试验采用不同浓度的松针多糖作用于正常的和经LPS刺激的小鼠腹腔巨噬细胞，设空白对照组（加入100 μL RPMI-1640培养基）、阳性对照组（加入100 μL终浓度为5 μg/mL的LPS）、松针多糖组（分别加入100 μL 25、50、100、200 μg/mL的松针多糖）和LPS+松针多糖组（加入与松针多糖组相同浓度的松针多糖和终浓度为5 μg/mL的LPS，液体终体积为200 μL）。噻唑蓝（MTT）比色法检测细胞活力，中性红吞噬试验检测巨噬细胞吞噬能力，Griess法检测一氧化氮（NO）的分泌量，酶联免疫吸附测定（ELISA）法检测巨噬细胞培养上清液中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）的分泌量。结果表明：1）对空白对照组相比，各松针多糖组巨噬细胞相对增殖率均显著或极显著提高（P < 0.05或P < 0.01），50、100和200 μg/mL松针多糖组巨噬细胞中性红吞噬率显著或极显著提高（P < 0.05或P < 0.01），各LPS+松针多糖组巨噬细胞中性红吞噬率均极显著提高（P<0.01），200 μg/mL松针多糖组巨噬细胞NO分泌量极显著提高（P<0.01），50、100和200 μg/mL松针多糖组巨噬细胞TNF-α和IL-1β分泌量显著或极显著提高（P < 0.05或P < 0.01），50、100和200 μg/mL松针多糖组巨噬细胞IL-10分泌量极显著降低（P<0.01）。2）与阳性对照组相比，各松针多糖组巨噬细胞相对增殖率均极显著降低（P<0.01），100和200 μg/mL LPS+松针多糖组巨噬细胞中性红吞噬率极显著升高（P<0.01），50、100和200 μg/mL LPS+松针多糖组巨噬细胞NO分泌量极显著提高（P<0.01），各LPS+松针多糖组巨噬细胞TNF-α分泌量显著或极显著提高（P < 0.05或P < 0.01），50、100和200 μg/mL LPS+松针多糖组巨噬细胞IL-1β分泌量显著或极显著提高（P < 0.05或P < 0.01），100和200 μg/mL LPS+松针多糖组巨噬细胞IL-10分泌量极显著降低（P<0.01）。由此可见，松针多糖通过发挥其促炎作用调节巨噬细胞的免疫功能，进而增强机体抗疾病的能力。

This experiment was conducted to explore the immunoregulation effects of polysaccharides from pine needles on normal and LPS stimulated mouse peritoneal macrophages. Different concentrations of polysaccharides from pine needles were used in normal and LPS stimulated mouse peritoneal macrophages. The blank control group was added 100 μL RPMI-1640 medium, the positive control group was added 100 μL LPS which the final concentration was 5 μg/mL, the polysaccharides from pine needles groups were added 100 μL of 25, 50, 100, 200 μg/mL polysaccharides from pine needles, and the LPS+polysaccharides from pine needles groups were added the mixture of the same concentration as polysaccharides from pine needles groups and LPS solution which the final concentration was 5 μg/mL and the final volume of the liquid was 200 μL. The thiazolyl blue tetrazolium bromide (MTT) assay was used to tested the cell viability, and the macrophages phagocytic ability was tested by neutral red phagocytosis experiments, the secretory volume of nitrous oxide (NO) was measured by Griess reaction, the secretory volumes of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in culture supernatants were measured by enzyme-linked immunosorbent method (ELISA). The results showed that:1) compared with the blank control group, the relative proliferation rate of macrophages was significantly increased in all polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the neutral red phagocytic rate of macrophages was significantly increased in 50, 100 and 200 μg/mL polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the neutral red phagocytic rate of macrophages was significantly increased in all LPS+polysaccharides from pine needles groups (P<0.01), the NO secretory volume of macrophages was significantly increased in 200 μg/mL polysaccharides from pine needles group (P<0.01), the TNF-α and IL-1β secretory volumes of macrophages were significantly increased in 50, 100 and 200 μg/mL polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the IL-10 secretory volume of macrophages was significantly decreased in 50, 100 and 200 μg/mL polysaccharides from pine needles groups (P<0.01). 2) Compared with the positive control group, the relative proliferation rate of macrophages was significantly decreased in all polysaccharides from pine needles groups (P<0.01), the neutral red phagocytic rate of macrophages was significantly increased in 100 and 200 LPS+polysaccharides from pine needles groups (P<0.01), the NO secretory volume of macrophages was significantly increased in 50, 100 and 200 μg/mL LPS+polysaccharides from pine needles group (P<0.01), the TNF-α secretory volume of macrophages was significantly increased in all LPS+polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the IL-1β secretory volume of macrophages was significantly increased in 50, 100 and 200 μg/mL LPS+polysaccharides from pine needles groups (P < 0.05 or P < 0.01), the IL-10 secretory volume of macrophages was significantly decreased in 100 and 200 μg/mL LPS+polysaccharides from pine needles groups (P<0.01). It is concluded that polysaccharides from pine needles can play a role in proinflammatory, thereby regulate the immune function of macrophages, thus enhancing the ability of anti-disease.