繁殖母羊的氧化应激和氧化损伤研究

余思佳, 施东辉, 朱勇, 徐建雄, 徐维娜, 张京

doi:10.3969/j.issn.1006-267x.2017.03.011

动物营养学报 >

2017 , Vol. 29 >Issue 3: 814 - 823

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.011

反刍与草食动物营养 Ruminant and herbivore nutrition

繁殖母羊的氧化应激和氧化损伤研究

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1. 上海交通大学农业与生物学院, 上海市兽医生物技术重点实验室, 上海 200240;
2. 上海市崇明县动物疫病预防控制中心, 上海 202150

余思佳(1992-),女,福建龙岩人,硕士研究生,研究方向为动物营养与饲料科学。E-mail:scarlettyu@yeah.net

收稿日期: 2016-09-02

网络出版日期: 2017-03-09

基金资助

公益性行业（农业）科研专项（201303143-06-01）

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A Research in Oxidative Stress and Damage of Breeding Goats

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1. Shanghai Key Laboratory of Veterinary Bio-Tech, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China;
2. Shanghai Chongming Animal Disease Prevention and Control Center, Shanghai 202150, China

Received date: 2016-09-02

Online published: 2017-03-09

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摘要

本试验旨在探究不同生殖阶段、胎次繁殖母羊的氧化应激及氧化损伤状态。试验1选取体重、体况相近的2~3胎空怀期、妊娠期（30、60、120 d）、分娩及哺乳期（21 d）的崇明白山羊各5只；试验2选取后备、1胎、2~3胎、4~5胎、6胎及以上的崇明白山羊母羊各5只。分别采集血样，测定血清自由基代谢指标[皮质醇、过氧化氢（H₂O₂）含量]、抗氧化指标[总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-Px）和超氧化物歧化酶（SOD）活性]、氧化损伤指标[丙二醛（MDA）、8-异前列腺素-F2α（8-ISO-PGF2α）、8-羟基脱氧鸟苷（8-OHDG）、蛋白质羰基化（PC）含量]。结果表明：试验1，从空怀期至哺乳期，血清皮质醇、H₂O₂含量呈先上升后降低的趋势，在分娩时达最高值，较空怀期显著提高（P<0.05）；血清T-AOC、SOD活性逐渐增强，且哺乳期21 d较空怀期显著提高（P<0.05）；血清MDA、8-ISO-PGF2α、PC含量先增加后趋于平稳，且在分娩时达最高，较空怀期显著提高（P<0.05），血清8-OHDG含量逐渐增加，哺乳期21 d达到最高，较空怀期显著提高（P<0.05）。试验2，随着母羊胎次的增加，血清中皮质醇含量无显著变化（P>0.05），4~5胎、6胎及以上血清H₂O₂含量显著高于其他胎次（P<0.05）；血清T-AOC无显著变化（P>0.05），血清SOD及GSH-Px活性逐级递减，6胎及以上较后备母羊下降显著（P<0.05）；血清MDA、8-ISO-PGF2α、8-OHDG和PC含量逐渐增加，6胎及以上显著高于其他胎次（P<0.05）。综上所述，伴随着妊娠的进行，母羊体内的自由基代谢逐渐增强，与此同时，体内的抗氧化水平也相应提高以维持氧化-抗氧化体系平衡，但在妊娠后期至哺乳期激增的活性氧自由基破坏了自由基稳衡体系，导致氧化应激和氧化损伤；随着母羊胎次的增加，抗氧化能力逐渐减弱，高胎次母羊氧化损伤严重。

关键词： 繁殖母羊; 妊娠; 胎次; 氧化应激

本文引用格式

余思佳, 施东辉, 朱勇, 徐建雄, 徐维娜, 张京 . 繁殖母羊的氧化应激和氧化损伤研究[J]. 动物营养学报, 2017 , 29(3) : 814 -823 . DOI: 10.3969/j.issn.1006-267x.2017.03.011

Abstract

This study was conducted to investigate the oxidative stress and damage status of breeding goats at different breeding stages and different parities. In test 1, Chongming white goats with similar body weight and physical condition at 2 to 3 parities at different breeding stages were used, and the stages were as follows:non-pregnant stage, pregnant stage (30, 60 and 120 d), the parturition day and lactation stage (21 d), and five from each were used; in test 2, Chongming white goats at different parities were used, and the parities were nulliparity,1 parity, 2 to 3 parities, 4 to 5 parities and 6 and above parities, and five from each were used. Blood sample was collected to analysis serum free radical metabolism indexes[cortisol and hydrogen peroxide (H₂O₂) contents], antioxidant indexes[total antioxidant capacity (T-AOC), and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD)] and oxidative damage indexes[maleic dialdehyde (MDA), 8-iso prostaglandin (8-ISO-PGF2α), 8-hydroxydeoxyguanosine (8-OHDG), and protein carbonyl (PC) contents]. The results showed as follows:in test 1, from non-pregnant stage to lactation stage, serum contents of cortisol and H₂O₂ were firstly increased and then decreased, and reached the highest values on the parturition day, which were significantly higher than those at non-pregnant stage (P<0.05); serum T-AOC and SOD activity were gradually increased, and lactation stage (21 d) was significantly higher than non-pregnant stage (P<0.05); serum contents of MDA, 8-ISO-PGF2α and PC were firstly increased and then tended to be stable, and reached the highest values on the parturition day, which were significantly higher than those at non-pregnant stage (P<0.05); serum 8-OHDG content was gradually increased, and reached the highest value at lactation stage (21 d), which was significantly higher than that at non-pregnant stage (P<0.05). In test 2, with the increase of parities, there was no significant changes of serum cortisol content (P>0.05), and serum H₂O₂ content at 4 to 5 parities and 6 and above parities was significantly higher than that at the other parities (P<0.05); there was no significant change of serum T-AOC (P<0.05), but serum activities of SOD and GSH-Px were gradually decreased, and 6 and above parities were significantly decreased compared with nulliparity (P<0.05); serum contents of MDA, 8-ISO-PGF2α, 8-OHDG and PC were gradually increased, and 6 and above parities were significantly higher than parities (P<0.05). In conclusion, with the development of pregnancy, free radical metabolism of goats is gradually enhanced, meanwhile, antioxidant capacity is also improved to keep the oxidant-antioxidant system balance; but due to the proliferation of reactive oxygen species from late-pregnant stage to lactation stage, the balance is broken, and strong oxidative damage is caused. with the increase of parities, the antioxidant capacity of goats get weak, high parities goats suffer strong oxidative damage.

Key words： breeding goat; pregnancy; parity; oxidative stress

参考文献

[1] MYATT L,CUI X L.Oxidative stress in the placenta[J].Histochemistry and Cell Biology,2004,122(4):369-382.

[2] MIN J,PARK B,KIM Y J,et al.Effect of oxidative stress on birth sizes:consideration of window from Mid pregnancy to delivery[J].Placenta,2009,30(5):418-423.

[3] MORRIS J M,GOPAUL N K,ENDRESEN M J R,et al.Circulating markers of oxidative stress are raised in normal pregnancy and pre-eclampsia[J].BJOG:An International Journal of Obstetrics and Gynaecology,1998,105(11):1195-1199.

[4] LITTLE R E,GLADEN B C.Levels of lipid peroxides in uncomplicated pregnancy:a review of the literature[J].Reproductive Toxicology,1999,13(5):347-352.

[5] 吴永霞,董国忠,贾亚伟.氧化应激对奶牛的危害及其防治[J].中国饲料,2011(4):32-35.

[6] KIM Y J,HONG Y C,LEE K H,et al.Oxidative stress in pregnant women and birth weight reduction[J].Reproductive Toxicology,2005,19(4):487-492.

[7] MIN J,PARK H,PARK B,et al.Paraoxonase gene polymorphism and vitamin levels during pregnancy:relationship with maternal oxidative stress and neonatal birthweights[J].Reproductive Toxicology,2006,22(3):418-424.

[8] 黄成竹,于倩楠,陶林林,等.氧化应激对断奶仔猪的影响以及氨基酸对氧化应激调控作用的研究[J].养猪,2013(1):49-51.

[9] 李永义,段绪东,赵娇,等.茶多酚对氧化应激仔猪生长性能和免疫功能的影响[J].中国畜牧杂志,2011,47(15):53-57.

[10] CELI P,DI TRANA A,CLAPS S.Effects of plane of nutrition on oxidative stress in goats during the peripartum period[J].The Veterinary Journal,2010,184(1):95-99.

[11] BROWN L,REGNAULT T,ROZANCE P,et al.Pregnancy and feto-placental growth:macronutrients[M]//SYMONDS M E,RAMSAY M M.Maternal-fetal nutrition during pregnancy and lactation.Cambridge:Cambridge University Press,2010.

[12] MUTLU B,BAS A Y,AKSOY N,et al.The effect of maternal number of births on oxidative and antioxidative systems in cord blood[J].The Journal of Maternal-Fetal & Neonatal Medicine,2012,25(6):802-805.

[13] 李桂玲.产程中静脉补充能量的效果研究[J].中国医药科学,2011,1(10):79,93.

[14] STADTMAN E R,LEVINE R L.Free radical-mediated oxidation of free amino acids and amino acid residues in proteins[J].Amino Acids,2003,25(3/4):207-218.

[15] MILLER J K,BRZEZINSKA-SLEBODZINSKA E,MADSEN F C.Oxidative stress,antioxidants,and animal function[J].Journal of Dairy Science,1993,76(9):2812-2823.

[16] 陈小连,孙婷婷,徐建雄.微生物源性抗氧化剂对大鼠抗氧化及损伤修复的作用[J].中国饲料,2010(22):11-15.

[17] HUNG T H,LO L M,CHIU T H,et al.A longitudinal study of oxidative stress and antioxidant status in women with uncomplicated pregnancies throughout gestation[J].Reproductive Sciences,2010,17(4):401-409.

[18] 徐又先.氧化应激在妊娠方面的研究进展[J].中国优生与遗传杂志,2012,20(12):7-9.

[19] JUNTUNEN K,KIRKINEN P,KAUPPILA A.The clinical outcome in pregnancies of grand grand multiparous women[J].Acta Obstetricia et Gynecologica Scandinavica,1997,76(8):755-759.

[20] GOLALIZADEH F,SHOBEIRI F,RANJBAR A,et al.Maternal parity and blood oxidative stress in mother and neonate[J].Biotechnology and Health Sciences,2016,3(1):e34165.

[21] ZIOMKIEWICZ A,SANCILIO A,GALBARCZYK A,et al.Evidence for the cost of reproduction in humans:high lifetime reproductive effort is associated with greater oxidative stress in post-menopausal women[J].PLoS One,2016,11(1):e0145753.

[22] FINKEL T,HOLBROOK N J.Oxidants,oxidative stress and the biology of ageing[J].Nature,2000,408(6809):239-247.

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