螺旋藻对仔猪生长性能、免疫性能及粪便菌群的影响

盛清凯, 刘雪, 韩红, 杨赵军, 同海妮, 孙利芹, 朱昌雄

doi:10.3969/j.issn.1006-267x.2017.03.014

动物营养学报 >

2017 , Vol. 29 >Issue 3: 843 - 849

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.014

饲料营养 Feed science and technology

螺旋藻对仔猪生长性能、免疫性能及粪便菌群的影响

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1. 山东省农业科学院畜牧兽医研究所, 山东省畜禽疫病防治与繁育重点实验室, 济南 250100;
2. 中国农业科学院农业环境与可持续发展研究所, 北京 100081;
3. 山东美事达农牧科技有限公司, 济阳 264005;
4. 烟台大学生命科学学院, 烟台 264005

盛清凯(1971-),男,山东德州人,研究员,博士,主要从事动物营养与饲料科学研究。E-mail:qksheng@163.com

收稿日期: 2016-09-21

网络出版日期: 2017-03-09

基金资助

环保部水体污染控制与治理科技重大专项（2013ZX07103006-007）；山东省农业重大应用技术创新项目（鲁财农指[2016]36号）

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Effects of Spirulina on Growth Performance, Immune Function and Fecal Flora of Piglets

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1. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Animal Disease Control and Breeding, Jinan 250100, China;
2. Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
3. Shandong Meishida Animal Husbandry Science and Technology Co. Ltd., Jiyang 264055, China;
4. College of Life Sciences, Yantai University, Yantai 264005, China

Received date: 2016-09-21

Online published: 2017-03-09

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摘要

为开拓螺旋藻饲料资源，本试验研究了螺旋藻单独及其与含有枯草芽孢杆菌和乳酸菌的益生菌联用对仔猪生长性能、免疫性能以及粪便菌群的影响。按2×2析因试验设计，将80头平均体重为（8.85±0.20）kg、口蹄疫（FMD）母源抗体为阴性的断奶仔猪随机均分为对照组、螺旋藻组（添加0.5%的螺旋藻）、益生菌组（添加0.5 kg/t的益生菌）以及藻菌组（添加0.5%的螺旋藻与0.5 kg/t的益生菌），每组5个重复，每个重复4头猪，饲粮以粉料形式饲喂。试验期28 d。第8日免疫猪O型及A型FMD疫苗，第7日及第28日采集血清及新鲜粪便。结果显示：1）螺旋藻显著提高了仔猪平均日增重（P<0.05），显著降低了料重比（P<0.05）。2）第7日和第28日螺旋藻对O型和A型FMD抗体滴度皆无显著影响（P>0.05）。3）第28日螺旋藻极显著提高了仔猪血清免疫球蛋白G（IgG）、免疫球蛋白M（IgM）及补体3含量（P<0.01），对血清补体4含量无显著影响（P>0.05）。4）第7日和第28日螺旋藻对仔猪粪便中乳酸菌、大肠杆菌数量无显著影响（P>0.05）。除第28日IgG、IgM和补体3含量外，螺旋藻与益生菌无互作（P>0.05）。结果表明，FMD疫苗免疫期间饲粮中添加0.5%的螺旋藻可以促进仔猪的生长，与益生菌配伍能够提高仔猪的体液免疫性能。

关键词： 螺旋藻; 益生菌; 仔猪; 口蹄疫; 互作

本文引用格式

盛清凯, 刘雪, 韩红, 杨赵军, 同海妮, 孙利芹, 朱昌雄 . 螺旋藻对仔猪生长性能、免疫性能及粪便菌群的影响[J]. 动物营养学报, 2017 , 29(3) : 843 -849 . DOI: 10.3969/j.issn.1006-267x.2017.03.014

Abstract

The effects of Spirulina alone or combined with probiotics (Bacillus subtilis and Lactobacilli) on growth performance, immune function and fecal flora of piglets were investigated as means to explore Spirulina feed resource. According to a 2×2 factorial design, eighty weaning piglets with the average body weight of (8.85±0.20) kg and negative foot-and-mouth disease (FMD) maternal antibody were assigned to control group, Spirulina group (plus 0.5% Spirulina), probiotics group (plus 0.5 kg/t probiotics) and Spirulina probiotics group (plus 0.5% Spirulina and 0.5 kg/t probiotics) randomly. Each group included 5 pens with 4 pigs in each pen. Piglets were fed powder feed and the whole test lasted for 28 days. On the 8th day, all piglets were vaccinated with O-type and A-type FMD vaccine, and on the 7th and 28th days, serum and fresh feces were collected. The results showed as follows:1) Spirulina significantly increased average daily gain and decreased the ratio of feed to gain of piglets (P<0.05). 2) On the 7th and 28th days, Spirulina did not influence O-type and A-type FMD antibody titers (P>0.05). 3) On the 28th day, Spirulina extremely significantly increased the serum contents of immunoglobulin (Ig) G, IgM and complement 3 (C3) (P<0.01), and did not affect the serum complement 4 content (P>0.05) of piglets. 4)On the 7th and 28th days, Spirulina did not affect Lactobacilli and E. coli amounts in feces (P>0.05). Interactions about serum IgG, IgM and C3 contents between the Spirulina and probiotics were significant on the 28th day (P<0.05), and the others were not (P>0.05). It is concluded that additional 0.5% Spirulina in the diet can promote growth and synergistically improve the humoral immune function combined with probiotics during the FMD vaccination of piglets.

Key words： Spirulina; probiotics; piglets; foot and mouth disease; interaction

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