沙葱黄酮对肉羊外周血淋巴细胞转化率和凋亡率的影响

木其尔, 王贵, 敖长金, 王特日格乐, 陈仁伟, 萨茹丽

doi:10.3969/j.issn.1006-267x.2017.03.017

动物营养学报 >

2017 , Vol. 29 >Issue 3: 866 - 873

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.017

饲料营养 Feed science and technology

沙葱黄酮对肉羊外周血淋巴细胞转化率和凋亡率的影响

展开

1. 内蒙古农业大学动物科学学院, 呼和浩特 010018;
2. 河套学院农学系, 临河 015000

木其尔(1989-),女,蒙古族,内蒙古赤峰人,博士研究生,从事动物营养与畜产品品质研究。E-mail:hamuqier1989@126.com

收稿日期: 2016-09-13

网络出版日期: 2017-03-09

基金资助

国家自然科学基金地区科学基金项目（31260558，31160474）；“十二五”国家科技支撑计划（2013BDA10B04）

收起

Effects of Flavonoids from Allium mongolicum Regel on Transformation Ratio and Apoptosis Ratio in Peripheral Blood Lymphocytes of Meat Sheep

Expand

1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Department of Agronomy, Hetao College, Linhe 015000, China

Received date: 2016-09-13

Online published: 2017-03-09

Fold

摘要

本试验旨在研究饲粮中添加沙葱黄酮对肉羊外周血淋巴细胞转化率和凋亡率的影响。选用60只6月龄左右、体重（39.9±3.2）kg的小尾寒羊为试验动物，按照出生月龄和体重相近的原则，随机分为4组，每组15只羊。对照组饲喂基础饲粮，3个试验组分别在基础饲粮中添加11、22、33 mg/kg的沙葱总黄酮。预试期为15 d，正试期为60 d。正试期第60天空腹颈静脉采血，采用噻唑蓝（MTT）法测定肉羊外周血淋巴细胞转化率，采用流式细胞仪测定细胞周期、细胞凋亡率。结果表明：1）饲粮添加11、22、33 mg/kg沙葱黄酮可提高肉羊外周血淋巴细胞转化率，其中33 mg/kg组显著高于对照组（P<0.05）；2）与对照组相比，饲粮添加33 mg/kg沙葱黄酮能够显著提高S期和G2/M期细胞比例（P<0.05）；3）与对照组和11 mg/kg组相比，22和33 mg/kg组外周血淋巴细胞凋亡率显著提高（P<0.05）。由此可见，饲粮中添加11~33 mg/kg沙葱黄酮可促进肉羊外周血淋巴细胞转化，并使细胞向DNA合成期转化，促进细胞分裂，对细胞凋亡有显著促进作用，以添加33 mg/kg沙葱黄酮的效果最佳。

关键词： 沙葱黄酮; 肉羊; 外周血淋巴细胞; 转化率; 细胞周期; 细胞凋亡

本文引用格式

木其尔, 王贵, 敖长金, 王特日格乐, 陈仁伟, 萨茹丽 . 沙葱黄酮对肉羊外周血淋巴细胞转化率和凋亡率的影响[J]. 动物营养学报, 2017 , 29(3) : 866 -873 . DOI: 10.3969/j.issn.1006-267x.2017.03.017

Abstract

This experiment was conducted to study the effects of flavonoids from Allium mongolicum Regel on transformation ratio and apoptosis ratio in peripheral blood lymphocytes of meat sheep. Sixty 6-month-old meat sheep with similar body weight[(39.9±3.2) kg] were randomly assigned to 4 groups with 15 sheep per group. Control group was fed a basal diet, and the 3 experimental groups were fed the basal diet supplemented with 11, 22 and 33 mg/kg flavonoids from Allium mongolicum Regel, respectively. Preliminary feeding period was 15 d, and the experimental period was 60 d. Blood was collected on the 60th day of experimental period before feeding via jugular venipuncture. Transformation ratio of lymphocytes was determined by thiazolyl blue method, and cell cycle and cell apoptosis ratio were determined using flow cytometry. The results showed as follows:1) dietary supplementation of 11, 22 and 33 mg/kg flavoniods from Allium mongolicum Regel could increase transformation ratio in peripheral blood lymphocytes, and 33 mg/kg group was significantly higher than control group (P<0.05); 2) compared with control group, dietary supplementation of 33 mg/kg flavoniods from Allium mongolicum Regel could significantly increase cell percentage in S phase and G2/M phase (P<0.05); 3) compared with control group and 11 mg/kg group, dietary supplementation of 22 and 33 mg/kg flavoniods from Allium mongolicum Regel could significantly increase apoptosis ratio in peripheral blood lymphocytes (P<0.05). In conclusion, dietary supplementation of 11 to 33 mg/kg flavonoids from Allium mongolicum Regel can promote transformation of peripheral blood lymphocytes of meat sheep, stimulate cell transformation to DNA synthesis period, promote cell mitosis, and had significant promotion effect on cell apoptosis, especially for the supplementation of 33 mg/kg flavonoids from Allium mongolicum Regel, which had the best effect.

Key words： Allium mongolicum Regel flavoniods; meat sheep; peripheral blood lymphocytes; transformation ratio; cell cycle; cell apoptosis

参考文献

[1] 陈山,满良,金山.蒙古高原民族植物学[J].植物科学进展,2000(3):245-251.

[2] 陈山.中国草地饲用植物资源[M].沈阳:辽宁民族出版社,1994:431.

[3] 蒋涛,敖长金.沙葱的研究进展[J].现代农业科技,2008(9):10-11,14.

[4] 斯琴巴特尔,刘新民.蒙古韭的营养成分及民族植物学[J].中国草地,2002,24(3):52-54.

[5] 巴俊杰,张春丽,高建萍,等.沙葱营养成分分析[J].内蒙古农业大学学报,2002,23(4):114-115.

[6] 敖长金.沙葱化学成分及其生物学功能研究进展[J].饲料工业,2010,31(18):1-5.

[7] 木其尔,敖长金,萨茹丽,等.沙葱总黄酮对肉羊抗氧化能力的影响[J].动物营养学报,2016,28(6):1823-1831.

[8] 王洪武,贾亮亮,徐媛青,等.淫羊藿总黄酮对环磷酰胺致免疫低下小鼠的免疫调节作用[J].天津医药,2010,38(12):1068-1071.

[9] RANJAN D,SIQUIJOR A,JOHNSTON T D,et al.The effect of curcumin on human B-cell immortalization by Epstein-Barr virus[J].The American Surgeon,1998,64(1):47-51.

[10] LEE S C,CHAN W K,LEE T W,et al.Effect of a prodrug of the green tea polyphenol (-)-epigallocatechin-3-gallate on the growth of androgen-independent prostate cancer in vivo[J].Nutrition and Cancer,2008,60(4):483-491.

[11] 萨茹丽.沙葱黄酮提取工艺优化、结构鉴定及其相关生物活性研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2014.

[12] 赵春艳.沙葱中黄酮类化合物的分离纯化、结构鉴定及其对小鼠免疫抗氧化机能影响的研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2008.

[13] FERENCZI K,BURACK L,POPE M,et al.CD69,HLA-DR and the IL-2R identify persistently activated T cells in psoriasis vulgaris lesional skin:blood and skin comparisons by flow cytometry[J].Journal of Autoimmunity,2000,14(1):63-78.

[14] LECKIE M J,JENKINS G R,KHAN J,et al.Sputum T lymphocytes in asthma,COPD and healthy subjects have the phenotype of activated intraepithelial T cells (CD69⁺ CD103⁺)[J].Thorax,2003,58(1):23-29.

[15] 李林,曾耀英,黄秀艳,等.黄芩苷对小鼠T淋巴细胞体外增殖和细胞周期的影响[J].细胞与分子免疫学杂志,2009,25(1):75-78.

[16] 包玲玲.沙葱黄酮类化合物对绵羊瘤胃发酵、内环境参数及纤维素降解的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2015.

[17] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.

[18] 陈清华,刘祝英,贺建华.牛膝多糖对仔猪淋巴细胞增殖作用和细胞因子分泌量的影响[J].动物营养学报,2008,20(6):712-717.

[19] GEETHA S,RAM M S,SINGH V,et al.Effect of seabuckthorn on sodium nitroprusside-induced cytotoxicity in murine macrophages[J].Biomed & Pharmacother,2002,56(9):463-467.

[20] 刘荣珍,李凤文,苏爱荣,等.沙棘对小鼠免疫调节作用的实验研究[J].中国热带医学,2005,5(9):1950-1951.

[21] 徐玮,王利华,聂宁.茶多酚对幼犬淋巴细胞转化率的影响[J].饲料工业,2009(1):58-59.

[22] 晨阳,杨丽敏,陶格斯,等.蒙药总黄酮对淋巴细胞转化和巨噬细胞吞噬功能的影响[J].山东医药,2010,50(4):45-46.

[23] 王雯婕,陈剑,刘小勇,等.白藜芦醇对视网膜色素上皮细胞增殖的影响[J].中国病理生理杂志,2014,30(10):1839-1844.

[24] 汤明惠,张兴夫,丹妮,等.甘氨酸对奶牛乳腺上皮细胞增殖与凋亡的影响[J].动物营养学报,2014,26(8):2162-2168.

[25] 慕静静,曾耀英,黄秀艳,等.山奈酚对小鼠T淋巴细胞体外活化、增殖和细胞周期的影响[J].细胞与分子免疫学杂志,2009,25(12):1106-1108,1111.

[26] 林长乐,曾耀英,曾祥凤,等.鹰嘴豆芽素A对小鼠T淋巴细胞体外活化增殖和细胞周期的影响[J].暨南大学学报:医学版,2007,28(2):115-119.

[27] OUYANG L,SHI Z,ZHAO S,et al.Programmed cell death pathways in cancer:a review of apoptosis,autophagy and programmed necrosis[J].Cell Proliferation,2012,45(6):487-498.

[28] GUERTLER P,BRAND I C,MEYER H H D,et al.Feeding genetically modified maize (MON810) to dairy cows:comparison of gene expression pattern of markers for apoptosis,inflammation and cell cycle[J].Journal für Verbraucherschutz und Lebensmittelsicherheit,2012,7(3):195-202.

[29] MEDJAKOVIC S,JUNGBAUER A.Red clover isoflavones biochanin A and formononetin are potent ligands of the human aryl hydrocarbon receptor[J].The Journal of Steroid Biochemistry and Molecular Biology,2007,108(1/2):171-177.

[30] 肖凤霞,邓韬,邓少东,等.毛橘红总黄酮对酒精性肝损伤大鼠肝细胞凋亡的影响[J].广东药学院学报,2012,28(3):316-318.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们