动物营养学报 >

2017 , Vol. 29 >Issue 3: 906 - 915

DOI: https://doi.org/10.3969/j.issn.1006-267x.2017.03.022

研究简报 Short communications

饲粮n-6/n-3多不饱和脂肪酸比值对冬毛期北极狐生长性能及肝脏脂肪酸代谢相关蛋白基因表达的影响

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  • 中国农业科学院特产研究所, 吉林省特种经济动物分子生物学省部共建实验室, 长春 130112
钟伟(1980-),女,吉林永吉人,副研究员,博士研究生,从事特种经济动物营养代谢研究。E-mail:zhongwei8015@163.com

收稿日期: 2016-08-11

  网络出版日期: 2017-03-09

基金资助

吉林省自然基金科学项目（20140101033JC）；中国农业科学院创新工程项目

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Effects of Dietary n-6/n-3 Polyunsaturated Fatty Acids Ratio on Growth Performance and Related Protein Gene Expression of Liver Fatty Acid Metabolism of Arctic Foxes During the Winter Fur-Growing Period

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  • State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Science, Chinese Academy of Agriculture Sciences, Changchun 130112, China

Received date: 2016-08-11

  Online published: 2017-03-09

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摘要

本试验旨在研究饲粮n-6/n-3多不饱和脂肪酸（PUFA）比值对冬毛期北极狐生长性能、肝脏脂肪酸组成及肝脏型脂肪酸结合蛋白（L-FABP）和脂肪酸转运蛋白（FATP）基因表达的影响。试验选取48只157日龄、平均体重为（5 658±47）g的健康雄性北极狐，随机分成4组，每组12个重复，每个重复1只。Ⅰ组饲粮中添加12.00%鱼油和2.00%豆油，n-6/n-3 PUFA比值为3.00；Ⅱ组饲粮中添加9.38%玉米油和4.62%豆油，n-6/n-3 PUFA比值为18.03；Ⅲ组饲粮中添加12.00%玉米油和2.00%豆油，n-6/n-3 PUFA比值为40.83；Ⅳ组饲粮中添加1.50%鱼油和12.50%玉米油，n-6/n-3 PUFA比值为136.36。各组饲粮除油脂组成和配比不同外，其他原料一致。预试期7 d，正试期40 d。结果表明：1）饲粮n-6/n-3 PUFA比值对冬毛期北极狐的平均日增重（ADG）、平均日采食量（ADFI）和料重比（F/G）有极显著影响（P<0.01）。Ⅰ和Ⅳ组的ADG极显著高于Ⅱ和Ⅲ组（P<0.01），Ⅰ、Ⅱ和Ⅳ组的ADFI极显著高于Ⅲ组（P<0.01），Ⅳ组的F/G极显著低于Ⅱ和Ⅲ组（P<0.01）。2）饲粮n-6/n-3 PUFA比值对冬毛期北极狐肝脏单不饱和脂肪酸（MUFA）、PUFA、n-3 PUFA和n-6 PUFA的含量有显著或极显著影响（P<0.05或P<0.01），对肝脏饱和脂肪酸（SFA）含量无显著影响（P>0.05）。Ⅰ和Ⅳ组肝脏n-3 PUFA含量极显著高于Ⅱ和Ⅲ组（P<0.01），Ⅱ和Ⅲ组肝脏n-6 PUFA含量极显著高于Ⅰ和Ⅳ组（P<0.01）。3）饲粮n-6/n-3 PUFA比值对冬毛期北极狐肝脏L-FABP mRNA相对表达量无显著影响（P>0.05），但极显著影响肝脏FATP mRNA相对表达量（P<0.01）。Ⅰ和Ⅳ组肝脏FATP mRNA相对表达量极显著高于Ⅱ和Ⅲ组（P<0.01）。由此可见，饲粮添加1.50%鱼油与12.50%玉米油的混合油脂，即饲粮n-6/n-3 PUFA比值为136.36时，上调了肝脏中FATP基因的表达，增加了肝脏长链脂肪酸的转运及利用效率，促进了冬毛期北极狐的生长。

关键词： n-6/n-3多不饱和脂肪酸比值; 北极狐; 肝脏; 脂肪酸; 肝脏型脂肪酸结合蛋白; 脂肪酸转运蛋白

本文引用格式

钟伟, 张婷, 罗靖, 岳志刚, 刘学庆, 樊燕燕, 孙皓然, 孙旭阳, 李光玉 . 饲粮n-6/n-3多不饱和脂肪酸比值对冬毛期北极狐生长性能及肝脏脂肪酸代谢相关蛋白基因表达的影响[J]. 动物营养学报, 2017 , 29(3) : 906 -915 . DOI: 10.3969/j.issn.1006-267x.2017.03.022

Abstract

This experiment was conducted to study the effects of dietary n-6/n-3 polyunsaturated fatty acids (PUFA) ratio on growth performance, liver fatty acid composition and liver-type fatty acid binding protein (L-FABP) and fatty acid transport protein (FATP) gene expression of Arctic foxes during the winter fur-growing period. Forty-eight 157-day-old male Arctic foxes with the average body weight of (5 658±47) g were randomly divided into 4 groups with 12 replicates per group and 1 fox per replicate, and they were fed experimental diets containing 12.00% fish oil and 2.00% soybean oil (group Ⅰ), 9.38% corn oil and 4.62% soybean oil (group Ⅱ), 12.00% corn oil and 2.00% soybean oil (group Ⅲ), 1.50% fish oil and 12.50% corn oil (group Ⅳ) with the n-6/n-3 PUFA ratios were 3.00, 18.03, 40.83 and 136.36, respectively. The oil composition and proportion of diets among groups were different, but the other ingredients were consistent. The experiment was 7 days for adaption and 40 days for trial period. The results showed as follows:1) dietary n-6/n-3 PUFA ratio extremely significantly affected average daily gain (ADG), average daily feed intake (ADFI) and the ratio of feed to gain (F/G) of Arctic foxes during the winter fur-growing period (P<0.01). The ADG in groups Ⅰ and Ⅳ was extremely significantly higher than that in groups Ⅱ and Ⅲ (P<0.01), the ADFI in groups Ⅰ, Ⅱ and Ⅳ was extremely significantly higher than that in group Ⅲ (P<0.01), and the F/G in group Ⅳ was extremely significantly lower than that in groups Ⅱ and Ⅲ (P<0.01). 2) Dietary n-6/n-3 PUFA ratio extremely significantly or significantly affected liver monounsaturated fatty acids (MUFA), PUFA, n-6 PUFA and n-3 PUFA contents of Arctic foxes during the winter fur-growing period (P<0.01 or P<0.05), but had no significant difference in liver saturated fatty acids (SFA) content (P>0.05). The liver n-3 PUFA content in groups Ⅰ and Ⅳ was extremely significantly higher than that in groups Ⅱ and Ⅲ (P<0.01), while the liver n-6 PUFA content in groups Ⅱ and Ⅲ was extremely significantly higher than that in groups Ⅰ and Ⅳ (P<0.01). 3) Dietary n-6/n-3 PUFA ratio did not significantly affect liver L-FABP mRNA expression level of Arctic foxes during the winter fur-growing period (P>0.05), but extremely significantly affected liver FATP mRNA expression level (P<0.01). The FATP mRNA expression level in groups Ⅰ and Ⅳ was extremely significantly higher than that in groups Ⅱ and Ⅲ (P<0.01). In conclusion, when the ratio of n-6/n-3 PUFA is 136.36 (adding mixed oils of 1.50% fish oil+12.50% corn oil in the diets), it raises the expression of liver FATP gene, promotes the transportation and utilization efficiency of fatty acids, and improves the growth of Arctic foxes during the winter fur-growing period.

Key words： n-6/n-3 PUFA ratio; Arctic fox; liver; fatty acids; liver-type fatty acid binding protein; fatty acid transport protein

参考文献

[1] 喻礼怀.饲粮脂肪酸ω-6/ω-3对鹅脂肪代谢影响及其分子机制的研究[D].博士学位论文.扬州:扬州大学,2012:7-21.

[2] 高巧仙,宋代军,靳露.饲粮n-6/n-3多不饱和脂肪酸比例对畜禽健康和产品品质的影响[J].动物营养学报,2013,25(7):1429-1436.

[3] LARSSON S C,KUMLIN M,INGELMAN-SUNDBERG M,et al.Dietary long-chain n-3 fatty acids for the prevention of cancer:a review of potential mechanisms[J].The American Journal of Clinical Nutrition,2004,79(6):935-945.

[4] 王远孝,张莉莉,王恬.不同油脂配比对黄羽肉鸡生产性能、屠宰性能和器官指数的影响[J].粮食与饲料工业,2010(2):42-45,52.

[5] SANZ M,LOPEZ-BOTE C J,MENOYO D,et al.Abdominal fat deposition and fatty acid synthesis are lower and β-oxidation is higher in broiler chickens fed diets containing unsaturated rather than saturated fat[J].The Journal Nutrition,2000,130(12):3034-3037.

[6] 于会民,李德发,管武太,等.不同脂肪对肉鸡营养素沉积、体组成和血清代谢物的影响[J].畜牧兽医学报,1998,29(4):304-314.

[7] 周萌,曹俊明,梁海鸥,等.饲料n-3/n-6脂肪酸比值对军曹鱼生长及鱼体组织脂肪酸组成的影响[J].广东农业科学,2006(12):77-81.

[8] ROUVINEN K.Dietary effects of omega-3 polyunsaturated fatty acids on body fat composition and health status of farm-raised blue and silver foxes[J].Acta Agriculturae Scandinavica,1991,41(4):401-414.  

[9] 张婷,罗婧,钟伟,等.饲粮脂肪水平对冬毛期银狐能量代谢、血清生化指标、肝脏脂肪酸组成及肝脏型脂肪酸结合蛋白基因表达的影响[J].动物营养学报,2016,28(2):618-626.

[10] KÄKELÄ R,PÖLÖNEN I,MIETTINEN M,et al.Effects of different fat supplements on growth and hepatic lipids and fatty acids in male mink[J].Acta Agriculturae Scandinavica,Section A:Animal Science,2001,51(4):217-223.  

[11] ROUVINEN K,KIISKINEN T.Influence of dietary fat source on the body fat composition of mink (Mustela vison) and blue fox (Alopex lagopus)[J].Acta Agriculturae Scandinavica,1989,39(3):279-288.  

[12] VRICHIERI G V,OGATA R T,ZIMMERMAN A W,et al.Fatty acid binding proteins from different tissues show distinct patterns of fatty acid interactions[J].Biochemistry,2000,39(24):7197-7204.  

[13] RICHIERI G V,OGATA R T,KLEINFELD A M.Equilibrium constants for the binding of fatty acids with fatty acid-binding proteins from adipocyte,intestine,heart,and liver measured with the fluorescent probe ADIFAB[J].The Journal of Biological Chemistry,1994,269(30):23918-23930.

[14] 耿业业.育成期蓝狐脂肪消化代谢规律的研究[D].博士学位论文.北京:中国农业科学院,2011:28-29.

[15] FEDIAF.Nutritional guidelines for complete and complementary pet food for cats and dogs[S].Bruxelles:European Pet Food Industry Federation,2011,8:14.

[16] 夏中生.饲粮中不同油脂对生长鸡组织脂质含量及其脂肪酸组成的影响[J].广西农业大学学报,1998,17(4):323-332.

[17] FRITSCHE K L,CASSITY N A,HUANG S C.Effect of dietary fat source on antibody production and lymphocyte proliferation in chickens[J].Poultry Science,1991,70(3):611-617.  

[18] 郭志有.多不饱和脂肪酸配比调控荣昌仔猪免疫应激的研究[D].硕士学位论文.重庆:西南大学,2011:25-41.

[19] 安文俊.日粮中添加不同配比油脂对肉鸡生产性能、肉品质及脂肪代谢影响的研究[D].硕士学位论文.南京:南京农业大学,2010:33-47.

[20] 左磊,李藏兰,赖长华.不同n-6/n-3多不饱和脂肪酸比值对断奶仔猪生长性能和免疫反应的影响[J].中国畜牧杂志,2010,46(23):48-50.

[21] 沈曼曼.ω-6、ω-3多不饱和脂肪酸及其比值对畜禽影响的研究进展[J].广东饲料,2012,21(12):32-35.

[22] SHANG X G,WANG F L,LI D F,et al.Effect of dietary conjugated linoleic acid on the fatty acid composition of egg yolk,plasma and liver as well as hepatic stearoyl-coenzyme A desaturase activity and gene expression in laying hens[J].Poultry Science,2005,84(12):1886-1892.  

[23] GATLIN L A,SEE M T,HANSEN J A.The effects of dietary fat sources,levels,and feeding intervals on pork fatty acid composition[J].Journal of Animal Science,2002,80(6):1606-1615.  

[24] GUDBJARNASON S,OSKARSDOTTIR G.Modification of fatty acid composition of rat heart lipids by feeding cod liver oil[J].Biochimica et Biophysica Acta:Lipids and Lipid Metabolism,1977,487(1):10-15.  

[25] KINSELLA J E.Seafoods and fish oils in human health and disease[M].New York:Marcel Dekker,1987:317.

[26] MATZINGER D,DEGEN L,DREWE J,et al.The role of long chain fatty acids in regulating food intake and cholecystokinin release in humans[J].Gut,2000,46(5):688-694.

[27] HIRSCH D,STAHL A,LODISH H F.A family of fatty acid transporters conserved from mycobacterium to man[J].Proceedings of the National Academy of Sciences of the United States of America,1998,95(15):8625-8629.  

[28] STORCH J,THUMSER A E A.The fatty acid transport function of fatty acid-binding proteins[J].Biochimica et Biophysica Acta:Molecular and Cell Biology of Lipids,2000,1486(1):28-44.  

[31] LOWE J B,SACCHETTINI J C,LAPOSATA M,et al.Expression of rat intestinal fatty acid-binding protein in Escherichia coli.Purification and comparison of ligand binding characteristics with that of Escherichia coli-derived rat liver fatty acid-binding protein[J].The Journal of Biological Chemistry,1987,262(12):5931-5937.
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